• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.205-209
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• 2003

Recently, we introduced a new method for rapid screening of bacterial species- or subspecies-specific DNA probes, named “inverted dot blot hybridization screening method”. We then applied this method to develop species- or strain- specific DNA probes for Prevotella intermedia and Prevotella nigrescens. In those studies, among 96 candidate DNA probes which were screened by the new method, 5 probes were confirmed as being putatively strain-specific : 3 probes for P. nigrescens 9336 (ATCC 33563), one for each p. intermedia ATCC 25611 and one for P. nigrescens G8-9K-3 (ATCC 49046). In the present study, we evaluated by Southern blot analysis a DNA probe Pi29-L, one of the 96 candidate probes described above, whether it is specific for the strain ATCC 25611 off. intermedia. Our data show that the probe Pi29-L is potentially P. intermedia ATCC 25611-specific, which can be useful for the detection and identification of the strain, particularly in maintenance of the strain.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.33 no.12
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• pp.1357-1365
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• 2009

In this study, we enhance the numerical approach of Lee et al.$^{(1)}$ to spherical indentation technique for property evaluation of hyper-elastic rubber. We first determine the friction coefficient between rubber and indenter in a practical viewpoint. We perform finite element numerical simulations for deeper indentation depth. An optimal data acquisition spot is selected, which features sufficiently large strain energy density and negligible frictional effect. We then improve two normalized functions mapping an indentation load vs. deflection curve into a strain energy density vs. first invariant curve, the latter of which in turn gives the Yeoh-model constants. The enhanced spherical indentation approach produces the rubber material properties with an average error of less than 3%.

• Journal of the Earthquake Engineering Society of Korea
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• v.21 no.1
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• pp.59-67
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• 2017

Due to a lack of the hoop action of lateral reinforcements the effective confining force in rectangular sections reduces compared to circular ones. Therefore, the stress-strain model obtained from the experimental data with circular sections overestimates the lateral confinement effect in rectangular sections, which evaluates seismic safety margin of overall structural system excessively. In this study experiments with laterally-confined square sections have been performed and the characteristic values composing stress-strain model have been analyzed. With introduction of section coefficients, in addition, the new unified stress-strain model applicable to square sections as well as circular ones has been proposed.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2006.06a
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• pp.34-39
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• 2006

An automated surface-strain measurement system, named ASIAS, was developed by using the image processing and stereo vision techniques in the previous studies by the corresponding author and his coworkers. This system has been upgraded mainly to improve the accuracy through image enhancement, sub-pixel measurement, surface smoothing, etc., since the first version was released. The present study has still more improved the convenience of users as well as the accuracy of measurement by processing high resolution images 8 mega pixels or more which can be easily obtained from a portable digital steal camera. It is proved that high resolution image processing greatly decreases the measurement error and gives strain data without considerable deterioration of accuracy even when the deformed grids to be measured and the master grids for camera calibration are captured together in the same image, making the whole process of strain measurement much simpler.

• Transactions of Materials Processing
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• v.5 no.4
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• pp.343-352
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• 1996

One of the preferred methods that can be used to verify the results of finite element analysis is to measure surface strains of the deformed part for purpose of direct comparison with simulation results. Instead of using the usual manual method the vision-based measurement method is capable of determining surface geometry and strain from the deformed grid pattern automatically with the help of a computer. To obtain strain distribution over an area, the coordinates of such a surface grid are determined from the multiple video images by applying the photogrammetry principle. Methods to improve the overall accuracy of the vision-based strain measurement system are explored and the possible accuracies that can be attained by such a measurement method are discussed. A major emphasis is placed on the initial grid application method its accuracy and ease of subsequent image processing. Finite element analyses of limiting dome height(LDH) test are carried out and the results of them are compared with exsperimen-tal data.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2002.05a
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• pp.159-162
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• 2002

In this paper the stress-strain curves of bearing steels at hot working conditions are obtained by compression test with a computer controlled servo-hydraulic Gleeble 3800 testing machine and elongations and reductions of area of the bearing steels are obtained by tensile test with a computer controlled servo-hydraulic Gleeble 1500 testing machine. These tests have been focused to obtain the flow stress data and optimal hot forging conditions under various conditions of strain rates and temperatures. The strain rate sensitivity exponent and reduction of area of the materials are evaluated. Experimental results are resented for various conditions of temperatures and strain rates.

• Proceedings of the KSME Conference
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• 2004.04a
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• pp.132-137
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• 2004

In this work, effects of hyper-elastic rubber material properties on the indentation load-deflection curve and subindenter deformation are first examined via [mite element (FE) analyses. An optimal data acquisition spot is selected, which features maximum strain energy density and negligible frictional effect. We then contrive two normalized functions. which map an indentation load vs. deflection curve into a strain energy density vs. first invariant curve. From the strain energy density vs. first invariant curve, we can extract the rubber material properties. This new spherical indentation approach produces the rubber material properties in a manner more effective than the common uniaxial tensile/compression tests. The indentation approach successfully measures the rubber material properties and the corresponding nominal stress.strain curve with an average error less than 3%.

• Structural Engineering and Mechanics
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• v.33 no.3
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• pp.325-337
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• 2009

Structural damage detection, damage localization and severity estimation of jacket platforms, based on calculating modal strain energy is presented in this paper. In the structure, damage often causes a loss of stiffness in some elements, so modal parameters; mode shapes and natural frequencies, in the damaged structure are different from the undamaged state. Geometrical location of damage is detected by computing modal strain energy change ratio (MSECR) for each structural element, which elements with higher MSECR are suspected to be damaged. For each suspected damaged element, by computing cross-modal strain energy (CMSE), damage severity as the stiffness reduction factor -that represented the ratios between the element stiffness changes to the undamaged element stiffness- is estimated. Numerical studies are demonstrated for a three dimensional, single bay, four stories frame of the existing jacket platform, based on the synthetic data that generated from finite element model. It is observed that this method can be used for damage detection of this kind of structures.

• Geomechanics and Engineering
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• v.4 no.1
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• pp.19-37
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• 2012

This paper presents the results of analytical and numerical analyses of the effects of performing a pressuremeter test or driving a pile in clay. The geometry of the problem has been simplified by the assumptions of plane strain and axial symmetry. Pressuremeter testing or installation of driven piles has been modelled as an undrained expansion of a cylindrical cavity. Stresses, pore water pressures, and deformations are found by assuming that the clay behaves like normally consolidated modified Cam clay. Closed-form solutions are obtained which allow the determination of the principal effective stresses and the strains around the cavity. The analysis which indicates that the intermediate principal stress at critical state is not equal to the mean of the other two principal stresses, except when the clay is initially isotropically consolidated, also permits finding the limit expansion and excess pore water pressures by means of the Almansi finite strain approach. Results are compared with published data which were determined using finite element and finite difference methods.

• Mycobiology
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• v.47 no.2
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• pp.173-179
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• 2019

Cladophialophora is characterized by simple hyphomycetes with brown hyphae that give rise to branched chains of pale brown conidia and shows affinities with the Herpotrichiellaceae. A fungal strain belonging to the genus Cladophialophora was isolated from soil in Daegu, Korea. This strain produces numerous greenish to dark black lanose aerial mycelia with hair like structures. It is morphological similar to C. chaetospira, C. inabaensis, and C. multiseptata; however, the conidiophores and conidia sizes of the newly isolated strain (KNU16-032) are clearly different from them. The novelty of the strain was also confirmed based on phylogenetic analysis using the data sets of the internal transcribed spacer region of and the partial sequence of 28S ribosomal DNA region along with the cultural characteristics. Because morphological observations and phylogenetic analysis indicated that the strain is distinct from previously known Cladophialophora species, we propose this species as a new species Cladophialophora lanosa sp. nov., and provide the detailed descriptions in this study.