Mycobiology

The Korean Society of Mycology (한국균학회)
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Cladophialophora lanosa sp. nov., a New Species Isolated from Soil

  • Das, Kallol (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Seung-Yeol (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Jung, Hee-Young (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
  • Received : 2018.11.22
  • Accepted : 2019.04.08
  • Published : 2019.06.01
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Abstract

Cladophialophora is characterized by simple hyphomycetes with brown hyphae that give rise to branched chains of pale brown conidia and shows affinities with the Herpotrichiellaceae. A fungal strain belonging to the genus Cladophialophora was isolated from soil in Daegu, Korea. This strain produces numerous greenish to dark black lanose aerial mycelia with hair like structures. It is morphological similar to C. chaetospira, C. inabaensis, and C. multiseptata; however, the conidiophores and conidia sizes of the newly isolated strain (KNU16-032) are clearly different from them. The novelty of the strain was also confirmed based on phylogenetic analysis using the data sets of the internal transcribed spacer region of and the partial sequence of 28S ribosomal DNA region along with the cultural characteristics. Because morphological observations and phylogenetic analysis indicated that the strain is distinct from previously known Cladophialophora species, we propose this species as a new species Cladophialophora lanosa sp. nov., and provide the detailed descriptions in this study.

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References

  1. de Hoog GS, Queiroz-Telles F, Haase G, et al. Black fungi: clinical and pathogenic approaches. Med Mycology. 2000;38:243-250. https://doi.org/10.1080/mmy.38.s1.243.250
  2. Crous PW, Schubert K, Braun U, et al. Opportunistic, human-pathogenic species in the Herpotrichiellaceae are phenotypically similar to saprobic or phytopathogenic species in the Venturiaceae. Stud Mycol. 2007;58:185-217. https://doi.org/10.3114/sim.2007.58.07
  3. Horre R, de Hoog GS. Primary cerebral infections by melanized fungi: a review. Stud Mycol. 1999;43:176-193.
  4. Badali H, de Hoog GS, Curfs-Breuker I, et al. Use of amplified fragment length polymorphism to identify 42 Cladophialophora strains related to cerebral phaeohyphomycosis with in vitro antifungal susceptibility. J Clin Microbiol. 2010;48:2350-2356. https://doi.org/10.1128/JCM.00653-10
  5. Badali H, Gueidan C, Najafzadeh MJ, et al. Biodiversity of the genus Cladophialophora. Stud Mycol. 2008;61:175-191. https://doi.org/10.3114/sim.2008.61.18
  6. Lastoria C, Cascina A, Bini F, et al. Pulmonary Cladophialophora boppii infection in a lung transplant recipient: case report and literature review. J Heart Lung Transplant. 2009;28:635-637. https://doi.org/10.1016/j.healun.2009.02.014
  7. Eriksson OE, Baral H-O, Currah RS, et al. Outline of Ascomycota-2003. Myconet. 2003;9:1-89.
  8. Abliz P, Fukushima K, Takizawa K, et al. Identification of pathogenic dematiaceous fungi and related taxa based on large subunit ribosomal DNA D1/D2 domain sequence analysis. FEMS Immunol Med Microbiol. 2004;40:41-49. https://doi.org/10.1016/S0928-8244(03)00275-X
  9. Caligiorne RB, Licinio P, Dupont J, et al. Internal transcribed spacer rRNA gene-based phylogenetic reconstruction using algorithms with local and global sequence alignment for black yeasts and their relatives. J Clin Microbiol. 2005;43:2816-2823. https://doi.org/10.1128/JCM.43.6.2816-2823.2005
  10. Muller E, Petrini O, Fisher PJ, et al. Taxonomy and anamorphs of the Herpotrichiellaceae with notes on generic synonymy. Trans Br Mycol Soc. 1987;88:63-74. https://doi.org/10.1016/S0007-1536(87)80186-9
  11. Untereiner WA. Capronia and its anamorphs: exploring the value of morphological and molecular characters in the systematics of the Herpotrichiellaceae. Stud Mycol. 2000;45:141-149.
  12. Obase K, Douhan GW, Matsuda Y, et al. Cladophialophora floridana and Cladophialophora tortuosa, new species isolated from sclerotia of Cenococcum geophilum in forest soils of Florida, USA. Mycoscience. 2016;57:26-34. https://doi.org/10.1016/j.myc.2015.07.005
  13. Gams W, Verkley GJM, Crous PW. CBS course of mycology. 5th ed. Utrecht (Netherlands): Centraalbureau voor Schimmelcultures; 2007.
  14. White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York (NY): Academic Press, Inc.; 1990. p. 315-322.
  15. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol. 1990;172:4238-4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  16. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16:111-120. https://doi.org/10.1007/BF01731581
  17. Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33:1870-1874. https://doi.org/10.1093/molbev/msw054
  18. Usui E, Takashima Y, Narisawa K. Cladophialophora inabaensis sp. nov., a new species among the dark septate endophytes from a secondary forest in Tottori, Japan. Microbes Environ. 2016;31:357-360. https://doi.org/10.1264/jsme2.ME16016
  19. Usuki F, Narisawa K. A mutualistic symbiosis between a dark septate endophytic fungus, Heteroconium chaetospira, and a nonmycorrhizal plant, Chinese cabbage. Mycologia. 2007;99:175-184. https://doi.org/10.1080/15572536.2007.11832577
  20. Trejos A. Cladosporium carrionii n. sp. and the problem of Cladosporia isolated from chromoblastomycosis. Rev Biol Trop. 1954;2:75-112.
  21. Ridley MF. The natural habitat of Cladosporium carrionii, a cause of chromoblastomycosis in man. Aust J Dermatol. 1957;4:23-27. https://doi.org/10.1111/j.1440-0960.1957.tb01525.x
  22. Feng P-Y, de Hoog GS, Najafzadeh MJ, et al. Cladophialophora abundans, a novel species of Chaetothyriales isolated from the natural environment. Mycol Progress. 2014;13:381-391. https://doi.org/10.1007/s11557-013-0924-4
  23. Crous PW, Wingfield MJ, Guarro J, et al. Fungal planet description sheets: 154-213. Persoonia. 2013;31:188-296. https://doi.org/10.3767/003158513X675925

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