Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Identification of Prevotella intermedia ATCC 25611 Using Pi29-L DNA Probe.

Pi29-L DNA 프로브를 이용한 Prevotella intermedia ATCC 25611의 동정

  • 국중기 (조선대학교 치과대학 구강생화학 교실) ;
  • 백동헌 (단국대학교 치과대학 미생물 및 면역학 교실)
  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Recently, we introduced a new method for rapid screening of bacterial species- or subspecies-specific DNA probes, named “inverted dot blot hybridization screening method”. We then applied this method to develop species- or strain- specific DNA probes for Prevotella intermedia and Prevotella nigrescens. In those studies, among 96 candidate DNA probes which were screened by the new method, 5 probes were confirmed as being putatively strain-specific : 3 probes for P. nigrescens 9336 (ATCC 33563), one for each p. intermedia ATCC 25611 and one for P. nigrescens G8-9K-3 (ATCC 49046). In the present study, we evaluated by Southern blot analysis a DNA probe Pi29-L, one of the 96 candidate probes described above, whether it is specific for the strain ATCC 25611 off. intermedia. Our data show that the probe Pi29-L is potentially P. intermedia ATCC 25611-specific, which can be useful for the detection and identification of the strain, particularly in maintenance of the strain.

Keywords

References

  1. J. Periodontol v.68 Putative periodontal pathogens in subgingival plaque of young adults with and without early-onset periodontitis Albandar,J.M.;L.J.Brown;H.Loe
  2. Oral Microbiol. Immunol. v.11 Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions Ashimoto,A.;C.Chen;I.Bakker;J.Slots
  3. J. Korean Acad. Periodontol. v.32 Study on development of DNA probe for identification of Prevotella intermedia G8-9K-3 Bak,J.S.;S.H.Kim;D.K.Kim;J.H.Seong;B.O.Kim;M.K.Kim;J.K.Kook
  4. Oral Microbiol. Immunol. v.6 Use of randomly cloned DNA fragments for the identification of oral spirochetes DiRienzo,J.M.;S.Cornell;H.Boehringer
  5. J. Clin. Periodontol. v.15 The predominant culturable microbiota of active and inactive lesions of destructive periodontal diseases Dzink,J.L.;S.S.Socransky;A.D.Haffajee
  6. J. Clin. Periodontol. v.12 Gram negative species associated with active destructive periodontal lesions Dzink,J.L.;A.C.Tanner;A.D.Haffajee;S.S.Socransky
  7. Int. J. Syst. Bacteriol. v.45 Confirmation of the species Prevotella intermedia and Prevotella nigrescens Frandsen,E.V.;K.Poulsen;M.Kilian
  8. Oral Microbiol. Immunol. v.7 Phenotypic characteristics and DNA relatedness in Prevotella intermedia and similar organisms Fukushima,H.;H.Moroi;J.Inoue;T.Onoe;T.Ezaki;E.Yabuuchi;K.P.Leung;C.B.Walker;W.B.Clark;H.Sagawa
  9. J. Korean Acad. Periodontol. v.32 Study on isolation of Prevetella nigrescens 9336-specific DNA probe using random cloning method Gang,S.W.;S.H.Kim;D.K.Kim;J.H.Seong;B.O.Kim;J.J.Han;J.K.Kook
  10. J. Periodontol. v.65 Characterization of Prevotella intermedia and Prevotella nigrescens isolates from periodontic and endodontic infections Gharbia,S.E.;M.Haapasalo;H.N.Shah;A.Kotiranta;K.Lounatmaa;M.A.Pearce;D.A.Devine
  11. Monoclonal Antibodies Against Bacteria v.I Monoclonal antibodies to characterize the antigenic heterogeneity of Bacteroides intermedius Gmar,R.;C.Wyss;A.J.L.Macario(ed.);E.Conway de Macario(ed.)
  12. J. Periodontol. v.71 A rapid DNA probe method for detection of Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans Komiya,A.;T.Kato;T.Nakagawa;A.Saito;J.Takahashi;S.Yamada;K.Okuda
  13. FEMS Microbiol. Lett. v.219 A new method for rapid screening of bacterial species- or subspecies-specific DNA probes Kook,J.K;M.K.Kim;J.H.Seong;D.K.Kim;B.O.Kim;J.C.Park;K.K.Kim;S.J.Choe;B.M.Min
  14. J. Korean Acad. Dent. Health. v.26 Cloning of DNA probes for Campylobacter rectus ATCC 33238 Kook,J.K.;H.K.Jung;J.H.Seong;J.B.Son;B.O.Kim;D.K.Kim
  15. J. Microbiol. Biotechnol. v.13 Cloning of a potentially strain-specific DNA probe of Prevotella intermedia ATCC 25611 by inverted dot blot hybridization screening method Kook,J.K.;J.J.Hna;H.S.Kim;J.H.Seong;D.K.Kim;D.H.Baek;S.J.Choe
  16. Bergey's Manual of Systematic Bacteriology(2nd ed.) v.1 Identification of Procaryotes Krieg,N.R.;G.Garrity(ed.)
  17. FEMS Immunol. Med. Microbiol. v.6 Pigmented Prevetella species in the periodontally healthy oral cavity Kononen,E.
  18. Appl. Environ. Microbiol. v.53 Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate Lippke,J.A.;M.N.Strzempko;F.F.Raia;S.L.Simon;C.K.French
  19. J. Periodontal. Res. v.22 Microbiology of periodontal disease Moore,W.E.
  20. J. Clin. Perodontol. v.18 The microflora of periodontal sites showing active destructive progression Moore,W.E.;L.H.Moore;R.R.Ranney;R.M.Smibert;J.A.Burmeister;H.A.Schenkein
  21. Oral Microbiol. Immunol. v.11 Distribution and genetic analysis of oral Prevotella intermedia and Prevotella nigrescens Matto,J.;M.Saarela;B. von Troil-Linden;E.Kononen;H. Jousimies-Somer;H.Torkko;S.Alaluusua;S.Asikainen
  22. Infect. Immun. v.56 Serological studies of oral Bacteroides intermedius Nakazawa,F.;J.J.Zambon;H.S.Reynolds;R.J.Genco
  23. Molecular cloning: a laboratory manual(2nd ed.) Sambrook,J.;E.F.Fritsch;T.Maniatis
  24. J. Periodont. Res. v.19 Distribution of certain subgingival microbial species in selected periodontal conditions Savitt,E.D.;S.S.Socransky
  25. Prevotella nigrescens sp. nov. Int. J. Syst. Bacteriol. v.42 Biochemical and chemical studies on strains designated Prevotella intermedia and proposal of a new pigmented species Shah,H.N.;S.E.Gharbia
  26. Clin. Infect. Dis. v.20 no.suppl. 2 Detection of putative periodontal pathogens in subgingival by 16S ribosomal DNA amplification with the polymerase chain reaction Slots,J.;A.Ashimoto;M.J.Flynn;G.Li;C.Chen
  27. J. Appl. Bacteriol. v.53 Deoxyribonucleic acid among strains of Bacteroides melaninogenicus and related species van Steenbergen,T.J.;C.A.Vlaanderen;J. de Graaff
  28. J. Periodontol. v.62 Microflora and bacterial DNA restriction enzyme analysis in young adults with periodontitis van Steenbergen,T.J.;U. Van der Velden;F.Abbas;J.de Graaff