• Title/Summary/Keyword: stock culture

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Prospects and Challenges of Lab-cultured Meat: A Comprehensive Review (실험실에서 제조되는 고기 '배양육'의 전망)

  • Zo, Sun-Mi;Seok, Yong-Joo;Han, Sung Soo;Choi, Soon-Mo
    • Textile Coloration and Finishing
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    • v.34 no.4
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    • pp.302-311
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    • 2022
  • Increase of population, lack of land, and decrease in fertility of soil have caused the limitation of food production across the globe. This leads to developing alternative foods, at the same time, vegan society have been got bigger. They argue for animal's right to life and happiness, dissent from breeding and improving livestock, and proscribe eating meat. Emerging problem concerns their health as many turn to vegan society. Only intaking vegetable protein can cause health problems, leading to the development of novel food replacing meat. The most promising candidate is non-slaughtered cultured meat which can be produced in a laboratory. The cultured meat enables it to keep its texture, nutrient, and taste while also addressing the problems of breeding stock and slaughter. We have discussed the cultured meat industry from the perspective of a vegan society, and the prospects were considered from various aspects of awareness and preference.

A study on the policy of Korean Cultural Industry

  • Su, Shuai;Zhang, Fan
    • The Journal of Economics, Marketing and Management
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    • v.6 no.2
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    • pp.1-4
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    • 2018
  • Purpose - This research, based on the successful experience of Korea, especially of Korea government, which, though promulgating laws and making policies, plays an rather pivotal role in Korea cultural industry development process, in developing cultural industry, explores possibly successful path and pattern that is suitable for China cultural industry. Research design, data, and Methodology - The study conducted a survey on Korea's 2000-2011 year data. After empirically analyzing the data, we believe that cultural industry in Korea and China will maintain its growth momentum. Results - This study shows that China and Korea are both belong to the only cultural circle of Confucianism and Chinese character, therefore, to research the successful experience of Korea government in cultural industry development will do much good to better promoting the optimal development of China cultural industry. China can encourage private flow to take on enterprises. In terms of financing, diversification can be achieved, by the means of cash, land, intangible assets, technology, stock, bond, cultural lottery etc. Conclusions - Besides, to better the degree of the internationalization of the allocation of the cultural resources, China government can, under the condition that Chinese laws permit, encourage foreign capital to invest in Chinese cultural industry field.

Studies on the Contents of the Nutrients and the Nucleotides in Soup Stock During Cooking of Cow's Rumen-Reticulum (소양(Cow's Rumen-reticulum)영양성의 가열조리시 분과 맛성분의 용출에 관한 연구)

  • Lee, Yeon-Sook;Park, Dong-Yeon;Park, Jung-Sook
    • Journal of the Korean Society of Food Culture
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    • v.4 no.3
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    • pp.245-251
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    • 1989
  • To estimate the nutritional values and the optimum cooking method of the Cow's rumen-reticulum (tripe) which is a Korean traditional food, the nutrients of raw tissue of tripes, the changes in total N, ${\alpha}-amino$ N, calcium and phosphorus contents and nucleotides and their related compounds contents in soup stock prepared of tripe tissues according to various boiling time period with saucepot or pressure cooker and various ratios of the water to the tripe (wt/wt) were measured. No significant difference was observed in nutrients contents between rumen (1st stomach) and reticulum (2nd stomach) which contained 83% moisture, 0.4-0.5% ash, 3% fat, 13% protein, 50-56 mg% calcium, 75-76 mg% phosphorus. 75-77 mg% ${\alpha}-amino$ N on fresh weight basis. The results obtained show that the significant loss of nutrients observed after removing epitherial cell layer from tripe, and in aspect of the nutrients contents, the nucletides contents, and the sensory evaluation score in soup stock, the optimum cooking time period of tripe was 8 hours in boiling in saucepot and 1 or 2 hours in cooking in pressure cooker, and the ratio of the water to the tripe (wt/wt) was above ten.

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In vitro Multiplication through Single-Node Culture of Sea-Milkwort (Glaux maritima L.) (갯봄맞이(Glaux maritima L.) 실생의 단마디배양을 통한 기내증식)

  • Bae, Su-Ji;Kang, Beum-Chang;Jeong, Mihye;Kim, Soochong;Kim, Chang Kil;Han, Jeung-Sul
    • Horticultural Science & Technology
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    • v.34 no.3
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    • pp.461-471
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    • 2016
  • This study was conducted to establish an in vitro propagation system for sea-milkwort (Glaux maritima L.), which is an endangered coastal plant species with high horticultural value. Two phenotypes, 'Red type (RT)' and 'Pistachio type (PT)' based on the colors of stem and flower, were obtained from a personal horticulturist in 2009 and used for this study as plant materials. The stock plants showed typical morphologies in flower, capsule, and seed appearances as previously reported. Low temperature treatment at $4^{\circ}C$ for four or more weeks after in vitro sowing maximized seed germination percentage, indicating that imbibition of seed and subsequent low temperature treatment are crucial for its germination. The in vitro seedlings had phenotypic variation, falling into 'RT' and 'PT' classes like the stock plants. Although slight differences depending on genotype and medium were recognized, the fourth or fifth nodes detached from the in vitro seedlings revealed the best multiplication efficacy when estimated on the basis of total number of nodes of newly developed axillary shoots. In addition, the nodes from 'RT' and 'PT' regenerated the most shoots on medium supplemented with $0.5mg{\cdot}L^{-1}$ BA alone and $0.5mg{\cdot}L^{-1}$ BA plus $0.5mg{\cdot}L^{-1}$ IAA, respectively. The node culture-derived plantlets were well acclimatized in a culture room ex vitro and completed the pseudo-annual life cycle coincident with that in the natural salt march habitat with the current cultivation method of applying fresh water-irrigation under an inland environment. This work represents the first report of in vitro propagation of sea-milkwort. Thus, our study will contribute to exo-habitat conservation and natural habitat restoration of this endangered species in addition to development of a horticultural product.

Suppression of Powdery Mildew Using the Water Extract of Xylogone ganodermophthora and Aqueous Potassium Phosphonate Solution on Watermelon under Greenhouse Conditions (Xylogone ganodermophthora 배양체 추출물 및 아인산칼륨 수용액을 이용한 시설수박 흰가루병 발생 억제효과)

  • Kang, Hyo-Jung;Kim, Youngsang;Kim, Taeil;Jeong, Taek Ku;Han, Chong U;Nam, Sang Young;Kim, Ik-Jei
    • Research in Plant Disease
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    • v.21 no.4
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    • pp.309-314
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    • 2015
  • Xylogone ganodermophthora (Xg) is an ascomycetous fungus that causes yellow rot on cultivated Ganoderma lucidum. Previously, we reported in vitro antifungal activities of a Xg culture extract against several watermelon pathogens. In 2014, we conducted greenhouse experiments to evaluate the control efficacy of a water extract of cultured Xg on watermelon powdery mildew (WPM). The test material (stock solution, ca. $4,000{\mu}g/ml$) was prepared by an autoclaved Xg culture in water at a ratio of 800 g of culture per 6 liter of water, and then filtering it through filter paper. Six foliar applications of the solutions (diluted 100- and 1,000-fold) significantly suppressed the formation of conidiophores and conidia. The inhibitory effect of aqueous potassium phosphonate solution on the disease and its phytotoxicity was tested. Phytotoxicity on watermelon plants was observed at concentrations of 1,000 and $2,000{\mu}g/ml$ as irregular brownish spots. The control efficacies against WPM were 91.9% at $2,000{\mu}g/ml$, 64.9% at $1,000{\mu}g/ml$, and 62.2% at $500{\mu}g/ml$.

효율적인 생식선 카이메라 생산을 위한 최적 조건 확립에 관한 비교 연구

  • 김진남;박태섭;송권화;이영목;권혁모;한재용
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2001.11a
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    • pp.71-73
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    • 2001
  • In this study, we could improve transmission efficiency of germline chimeras by transfer of gonadal PGCs (gPGCs) cultured in vitro. Of hatched recipient chicks, 301 chickens (141 males and 160 females) were brought up to sexual maturity and these WLs (KOC) were mated with KOCs for testcross, resulting in 27 germline chimeras (15 males and 12 females) identified by black feather color of their progenies. The production efficiency of germline chimera production of experimental groups was observed (P=0.6831). The average transmission efficiency of proven germline chimeras was 0.6 ∼56.5% (15.0% on average). The transmission efficiency of experimental group which were transferred 10-days cultured gPGCs without Ficoll treatment was highest (49.7%) and that of experimental stock which transferred non-cultured gPGCs with Ficoll treatment was lowest (0.6%). The duration of in vitro culture before transferring was significantly important for the high efficiency of germline transmission. Transferring 10-days cultured gPGCs made the transmission efficiency higher rather than transferring non-cultured and 5-days cultured gPGCs, 50 times and 10 times, respectively (p<0.0001). However, Ficoll treatment for increasing the population ratio of gPGCs negatively affected the transmission efficiency and the effects of sexuality and the reciprocal interaction between treatments showed no significant differences. These findings demonstrated that the crucial factors for improving the germline transmission were the duration of in vitro culture prior to transfer. Thus, we developed the complete system for production of germline chimera using cultured gPGCs with highly improved efficiency and this system would be useful for genetic manipulation and obtaining the transgenic aves.

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Research on the Effect of Gardeniae Fructus on Regulatory T Cell Stimulation (조절 T세포에 미치는 치자(梔子)의 효과)

  • Seo, San;Jung, Hee-Jae;Jung, Sung-Ki
    • The Journal of Internal Korean Medicine
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    • v.31 no.2
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    • pp.189-200
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    • 2010
  • Objectives : Regulatory T cells can reduce inflammation and allergic reactions through their inhibitory functions. Gardeniae Fructus(GF) is a Heat-clearing herb used in traditional Korean medicine, and a wide range of studies on its antiinflammatory effects are being carried out. The authors investigated the effect that Gardeniae Fructus has on regulatory T cells. Methods : The authors screened 14 herbs for their effects on regulatory T cells. 100mg of each herb were separately dissolved in 1ml of sterile saline and the supernatant was harvested after 10 minutes of centrifuge at 15,000 rpm. The supernatant was filtered through a 0.2 ${\mu}m$ syringe filter, and the resulting stock was refrigerated at $4^{\circ}C$. The stock was diluted before testing and used at a final concentration of $0.01{\mu}g/ml$. CD4+CD25+ T cells from healthy BALB/c spleens were used as natural regulatory T cells (nTreg), and CD4+CD25- T cells were used as reactive T cells. CD4+CD25+ and CD4+CD25- T cells were activated with anti-CD3e ($10{\mu}g/m{\ell}$)/anti-CD28 ($1{\mu}g/m{\ell}$) and cultured. IL-10 from supernatant of the culture medium was measured by IL-10 cytokine ELISA. The percentages, cell numbers, phenotype and function of CD4+CD25+ Treg cells were determined by flow cytometry. Results : Gardeniae Fructus was shown to be the most potent herb among the 14 herbs tested for suppressing CD4+CD25- reactive T cell proliferation by stimulating CD4+CD25+ natural regulatory T cells. Gardeniae Fructus induces IL-10 secretion increase by stimulating CD4+CD25+ natural regulatory T cells, and indirectly suppresses CD4+CD25- reactive T cell proliferation through increasing CD25 (IL-2 receptor $\alpha$) expression and thus promoting bonding with IL-2. Gardeniae Fructus did not directly affect CD4+CD25- reactive T cell proliferation. Conclusions : Gardeniae Fructus suppressed reactive T cell proliferation through inducing increases in IL-10 secretion and CD25 (IL-2 receptor $\alpha$) expression.

Estimating the Determinants of Loan Amount of Housing Mortgage : A Panel Data Model Approach (주택 담보 가계 대출액 결정요인 추정에 관한 패널 데이터 모형 연구)

  • Kim, Hee-Cheul;Shin, Hyun-Cheul
    • Journal of the Korea Society of Computer and Information
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    • v.16 no.7
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    • pp.183-190
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    • 2011
  • Loan amount of housing mortgage is composed of various factors. This study paper studies focuses on estimating the determinants of a loan amount of housing mortgage. The region for analysis consist of seven groups, that is, metropolitan city (such as Busan, Daegu, Incheon, Gwangiu, Daejeon, Ulsan.) and Seoul. Analyzing period be formed over a 45 time points(2007. 01.~ 2010. 09). In this paper the dependent variable setting up loan amount of housing mortgage, explanatory(independent) variables are composed of the consumer price index, unemployment rate, average monthly household income per household, expenditure rate of health care, composite stock price index and overdue rate of household loans for commercial bank. In looking at the factors which determine loan amount of housing mortgage, evidence was produced supporting the hypothesis that there is a significant positive relationship between the consumer price index and unemployment rate. The study also produced evidence supporting the view that there is a significant negative relationship between expenditure rate of health care. The study found that average monthly household income per household, expenditure, composite stock price index and overdue rate of household loans for commercial bank were not significant variables. The implications of these findings are discussed for further research.

A Study on Factors Affecting the Usage of the Digital Copyright Exchange in Knowledge Service Convergence Era (지식서비스 융합시대 디지털저작권거래소 이용의도에 영향을 미치는 요인에 관한 연구)

  • Lee, Jung-Jae;Han, Kyeong-Seok
    • Journal of Digital Convergence
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    • v.10 no.6
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    • pp.153-158
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    • 2012
  • Digital contents market has been drastically increasing under Clouding Computing and Smart Phone environment. The Contents industry is going to be more essential portion than manufacture or service industry in the near future. One good example of high value-added contents is the Harry Potter series which exceeds 10 years' net profit of Hundai automobile company. This one example shows us very well that digital contents market as a core-engine for culture industry development is economically influential and attractive as well. This study focuses on activation of Digital Copyright Exchange in knowledge service convergence era. First, it examines other services like stock exchange, electronic documents and authentication certificate etc. which are similar to DCE service. Then it analyzes what factors make copyright industry have interested in the DCE the through technology acceptance model. Based on the results of the analysis, finally, this study evaluates how the DCE contributes to copyright industry overall including fair utilization of works.

A Study on the displacement characteristics of suspension elements for KTX (고속철도차량 현가계요소 변위특성 연구)

  • Hur H.M.;Kwon S.T.;Lee C.W.;Kim H.J.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.378-382
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    • 2005
  • The opening of high speed railway upgraded our land transportation speed limit, causing lots of changes including living and culture and also paving the way for stepping up the railway technology. However, it is also true that we had a limit to adopt the existing railway system structured for 150km/h to the new structure requiring a higher speed of approximate 300km/h due to technological, based on the time and experience. More importantly, heading toward a step of operating such a high speed railway system, it has been practically and quickly proposed that the railway needs high speed railway engineering, maintenance technology of parts of the vehicles to have a stable maintenance foundation and localization of major parts. Therefore, this study was intended to research the actual displacement characteristics in runningg on an actual track for the purpose of developing the protective and maintenance technology of springs and dampers, which are core parts among suspension elements of a high speed railway vehicle. For this, it was researched the actual vehicle test and its interpretation centered on primary spring, which is used for the suspension system of a bogie, body-body dampers and body-bogie yaw damper. Also, to analyze the displacement characteristics of suspension system in the actual conditions of high speed railway vehicles, a vehicle‘s dynamic characteristics was analyzed and interpreted. At the same time, a tester for measuring the actual displacement of such suspension elements was designed and attached to actual vehicles, to measure the displacements that occur in running it on the Seoul-Busan line, one of major lines serviced by KTX. The displacement data gained from the test with actual vehicles was analyzed for its displacement distribution depending on the service sections and frequency, with which the valuable data necessary for any potential breakdown or maintenance in the future could be obtained.

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