• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.2206-2208
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• 2003

This paper is performed to find the effects on body heat by stimulating the skin with the heat of a moxibustion cauterizer and the Artemisia-lotion which cause the extension of the blood vessel. The Artemisia-lotion is made by extracting the vasodilator and antioxidant compounds from the $Artemisia-CH_2Cl_2$ fraction. The moxibustion cauterizer is constructed with a DC power supply and 3 heating electrodes with PTC(Positive Temperature Coefficients) thermistor. The stimulation temperature level of the moxibustion cauterizer is $45^{\circ}C\;-48^{\circ}C$. We gave the subjects the following stimulating conditions; only heat stimulation, only Artemisia -lotion stimulation, and both stimulations. The measurement of body heat was performed with a infrared thermography system . Finally we could estimate the effects of a betterment of circulation of the blood from $Artemisia-CH_2Cl_2$ fraction.

• Fisheries and Aquatic Sciences
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• 제18권3호
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• pp.325-332
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• 2015

An active stimulating method for juvenile fishes to drive escaping from mesh of the codend was examined by shaking canvas in the bottom trawl followed by shrimp beam trawl. Field fishing trials by a bottom trawl were carried out between the Geomoondo and Jejudo in west of South sea, Korea by conver-net methods to examine the effect on the reduction of juvenile fish as a discard catch by generating a shaking movement of the codend using two pieces of asymmetrical semi-circular canvas. The mean period of the shaking motion with the round canvas was 10-15 s, and the range of amplitude as a vertical depth change was up to 0.4-0.6 m when towing speed 3.4-4.3 k't as estimated by peak event analysis. The escape rate of juvenile fish in conver-net by total juvenile bycatch (codend and cover-net) in 14 trials increased from 20% in a steady codend to 34% using a shaking codend in the bottom trawl, while the marketing catch or total bycatch was similar between steady and shaking cod ends. There was no difference in the body size of the fish and species composition between the steady and shaking cod ends. Above results demonstrate a new method for bycatch reduction actually up to 18% using an active stimulating device, although further experiments are needed to increase an effective shaking motion of the codend in amplitude and period for more bycatch reduction.

• BMB Reports
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• 제38권2호
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• pp.253-257
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• 2005

In our previous study, Soamsan, a traditional Oriental medicine, was shown to enhance the induction of antigen-specific immune responses, and it was speculated that the enhancing activity might be closely associated with glycoproteins contained within the medicine. To elucidate this speculation, protein samples from each component, used in the preparation of Soamsan, were obtained and their immune stimulating activities were tested with mouse splenocytes. All the samples markedly enhanced the lymphocyte proliferation and cytokine secretion by the mouse splenocytes. In particular, the enhancement was significantly higher with the protein sample treatments than with those of the original crude sample. Furthermore, the pronase E- and $NaIO_4$-mediated inhibition of splenocyte-stimulation activity of the protein samples clearly supported that glycoproteins are the main class of active ingredients responsible for the lymphocyte stimulating activity of the samples. Consequently, our findings suggest that glycoproteins might have a pivotal role in Soamsan-mediated immune modulation, although the in vivo effect of the glycoproteins should be further elucidated.

• Biomolecules & Therapeutics
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• 제6권1호
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• pp.89-94
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• 1998

CJ50001 is a recombinant human granulocyte colony-stimulating facto, (rHuG-CSF) that stimulates the formation of neutrophils from bone marrow stem cells. It was produced in E. colt and purified through refolding and several processes. We produced CS970125(300) using purified C150001 and additives in order to test the stability of CJ50001. When CS970125(300) was stored at 50'S for more than 1 week, high molecular weight proteins were formed and those proteins were detected by non-reducing SDS-PAGE, gel filtration HPLC, and Western blot. Those proteins showed single band at the same position of CJ50001 in reducing SDS-PAGE. These data indicated that those high molecular weight proteins were the multimers of C150001. In biological assays, iu viro and in viro, the multimers did not have biological activity and inhibitory action to that of CJ 50001. The mutimers did not induce toxicity in mice and rats in acute toxicity test. These results suggest that if Cs970125(300) containing CJ50001 is stored at 5$0^{\circ}C$, CJ50001 will be the multimers that do not have biological activity and inhibitory effect to CJ50001 and do not induce acute toxicity.

• BMB Reports
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• 제39권1호
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• pp.84-90
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• 2006

During our search for macrophage stimulating compounds from medicinal plants, we isolated biopolymers from Acanthopanax sessiliflorus. Isolated fraction AS-5 showed maximum potential, and stimulated lysosonal enzymatic activity by 230% at $300\;{\mu}g/ml$. The nitric oxide (NO) producing ability of AS-5 $100\;{\mu}g/ml$ was $58\;{\mu}M$ when treated with interferon-$\gamma$ and lipopolysaccharide $20\;{\mu}g/ml$. The lymphocyte proliferating effects of isolated biopolymer fractions were also investigated. Highest lymphoproliferative activity (a 2.8-fold enhancement compared to saline treated group was exhibited by AS-3 at $200\;{\mu}g/ml$ followed by AS-5 and AS-6. The AS-3 fraction stimulated only T-lymphocytes and had little or no effect on B-lymphocyte proliferation. Partially methylated alditol acetates were prepared to elucidate the glycosyl linkage-compositions of the AS-3 and AS-5 biopolymers, and were analyzed by GC-MS. The AS-3 and AS-5 biopolymer fractions were found to contain 2,3,4-tri-O-methyl-D-glucitol, 2,3,4-tri-O-methyl-D-galacitol 3,4,6-tri-O-methyl-galacitol, 2-O-methyl-arabinitol and 2,4,6-tri-O-methyl-D-glucitol, 2,3,6-tri-O-methyl-D-galacitol linkages, respectively.

• 한국유기농업학회지
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• 제5권2호
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• pp.93-104
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• 1997

In order to investigate the growth response of grasses to chitosan solution amended soil were studied from the standpoint of estimating the growth stimulating adequate concentrations of chitosan solution amended soil in each grass. Three species in this experiment used were orchardgrass, tall fescue and reed canarygrass. Six different concentrations of chitosan solution amended soil were 0%(control), 0.01%, 0.05%, 0.1%, 0.5% and 1.0%, respectively. The resulte obtained were as follows ; 1. Leaf area(LA), dry weight of leaf(LW), dry weight of shoot(SHW), biological yield(BY), C/f ratio and T/R ratio were significantly different between species. 2. Growth stimulating effect by chitosan solution amended soil were found in plant length(PL) and T/R ratios of grasses. 3. Adequate concentrations of chitosan solution amended soil were different between species. The highest values of yield components and dry weight of plant parts were obtained at 0.01% in orchardgrass, 0,05% in reed canarygrass and 1.0% in tall fescue, respectively. 4. The growth response of grasses to chitosan solution amended soil were different between species. Thus, an increase in leaf area(LA) and dry weight of leaf(LW) by chitosan solution amended soil was mainly contributed to increase in dry weight of shoot(SHW) and biological yield(BY) in orchardgrass. Chitosan solution amended soil also stimulated growth of shoot and increased in biological yield(BY) in tall fescue. In reed canarygrass contributed to increase in C/F ratios. 5. Adequate concentrations of chitosan solution amended soil for an economical benefit of cultivation and dry matter production of grasses were ranged from 0.01% to 0.05% levels.

• Biomolecules & Therapeutics
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• 제10권3호
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• pp.137-141
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• 2002

Taurine, amino acid, chemically known as 2-amino ethane sulphonic acid was discovered more than two hundred years ago from ox bile. it is widely distributed in both mammals and nonmammals. It is found in considerably high amount in hUl11an: a normal adult of 70 kgs contains about 70 grams of taurine. Taurine with this much concentration, is involved in almost all life processes. Its deficiency causes several abnormalities in major organs like brain, eye and heart. Taurine-bone interaction is latest addition to its long list of actions. In bone cells, taurine is also found in high concentration. Taurine is found to help in enhancing the bone tissue formation which is evidenced by increased matrix formation and collagen synthesis. Besides stimulating the bone tissue formation, it also inhibits the bone loss through inhibiting the bone resorption and osteoclast formation. Thus, taurine acts as a double agent. In addition to these two major actions of taurine in bone, it also has beneficial effect in wound healing mld bone repair. Taurine possess radioprotective properties, too. As it is a naturally available molecule, it can be used as a preventive agent. Taurine has a potential to replace bisphosphonates which are currently in use for the inhibition of bone loss but this needs in depth study. As taurine is involved in bone formation and inhibition of bone loss, a detailed study can make it a single marker of bone metabolism. All these taurine-bone interaction is a symbol of their deep involvement but still require further extension to make taurine as a choice for tile sound bone health.

• Korean Journal of Acupuncture
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• 제22권3호
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• pp.53-61
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• 2005

Objectives : This study was designed to investigate the effect of thermal change on face, acupuncturing Yon-gok (KI2). The room temperature adjust $26^{\circ}C$ making use of air-conditioner. Methods : A Clinical study was done on 20 males and females who didn't have any disease. We used IR-5000 to observe the effects of acupuncture at face temperature. Results : Acupuncturing Yon-gok(KI2), the special region on face falls the temperature (n=20) from Avg.=$28.1400{\pm}0.2603^{\circ}C$ to Avg.=$27.4910{\pm}0.2539^{\circ}C$ the special region on chest falls the temperature (n=20) from Avg.=$26.9450{\pm}0.2591^{\circ}C$ to Avg.=$26.8690{\pm}0.2519^{\circ}C$ These effects result at the stimulating 'Hwa' point, one of five-shu-point one in the meridian, Yon-gok (KI2) is one of 'Hwa'. the stimulating 'Hwa' is able to cure fever, headache, and pains. and it has ablility of controlling body temperature, specially, upper region of whole human body. Conclusions : In conclusion. Acupuncturing Yon-gok (KI2) falls the temperature of region on face, Compared with the temperature of region on chest.

• 수산해양기술연구
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• 제50권3호
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• pp.342-350
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• 2014

An active stimulating device, such as a fluttering net or canvas attached to the end of a cod end generating a shaking motion, could help to encourage the escape of juvenile fish positioned passively near the net. Field fishing trials using a shrimp beam trawl were carried out to examine the effect on the reduction of juvenile fish or other discard catch by generating a shaking movement of the cod end using an unbiased cap-like round canvas. The mean period of the shaking motion with the round canvas was ~14 s, and the mean amplitude was 0.4 m as measured by peak event analysis and the global wavelet method. The bycatch of juvenile fish in 14 trials decreased by ~30% and by ~25% using a steady cod end for the total bycatch using a shaking cod end in the shrimp beam trawl, while the marketing catch was similar between steady and shaking cod ends. There was no difference in the body size of the shrimp or fish and species composition between the steady and shaking cod ends. Above results demonstrate a new method for bycatch reduction using an active stimulating device, although more detailed studies are needed.

• 대한약리학회지
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• 제10권2호
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• pp.43-54
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• 1974

The cactus family Cactaceae, numbering about 1,500 species, is a fleshy-stemmed perennial plant which is principally distributed in the south and north America. On the other hand, the cactus plant is presumed to be introduced into Korea in 1912-1945, thereafter it has been cultivated merely in favor of ornamentation with the exception of being occasionally used as medication among laymen. Opuntia elata which belongs to Opuntia genus, the Cactaceae family is one of the cacti being cultivated a great deal in Korea. Cho et. al. reported in 1974 that Opuntia dilenii manifested the cardiac inhibitory effect and oxytocic effect, and its mechanism might be partially due to the direct action. Besides this, there are few reports on the pharmacological research concerning Opuntia genus to be demonstrated in Korea. However, some other cacti have the remarkable pharmacological effects; the active ingredients, mescaline, anhalamine, anhalanine, etc. from Peyote cactus (Lophophora williamsii) belonging to Lophophora genus have the psychotomimetic and sympathomimetic effects, and the cardioactive glycosides from Cactus Grandifolius (Selenicereus grandiflorus) belonging to Cereus genus have the cardioactive and diuretic effects. The authors hereby inquired into this study to find out the propriety of the pharmacological properties of the ethanol extract of Opuntia elata (EX) on the heart, blood pressure, respiration, intestine and uterus in the experimental animals. The results of the experiment were as follows: 1. Administration of EX manifested the cardiac inhibitory effect caused by the negative inotropic action in the isolated heart of frog, and the pretreatment of atropine did not affect the inhibitory effect produced by EX. 2. Administration of EX manifested the transient hypotensive effect in the intact rabbit, and the pretreatment of atropine did not affect the hypotensive effect produced by EX. 3. Administration of the small dose of EX manifested no significant effect, but moderate dose or more the stimulating effect, and the large dose the asphyxia on the respiratory motility in the intact rabbit. 4. Administration of EX manifested the sustained augmentation of contractility in the excised duodenum of rabbit, and the pretreatment of atropine did not affect the stimulating effect produced by EX. 5. Administration of EX manifested the sustained augmentation of contractility in the excised pregnant uterus of rabbit, and the pretreatment of atropine and oxytocin did not affect the oxytocic effect produced by EX, but that of barium chloride more or less stimulated the oxytocic effect produced by EX.