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An Automatic Mobile Cell Counting System for the Analysis of Biological Image (생물학적 영상 분석을 위한 자동 모바일 셀 계수 시스템)

  • Seo, Jaejoon;Chun, Junchul;Lee, Jin-Sung
    • Journal of Internet Computing and Services
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    • v.16 no.1
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    • pp.39-46
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    • 2015
  • This paper presents an automatic method to detect and count the cells from microorganism images based on mobile environments. Cell counting is an important process in the field of biological and pathological image analysis. In the past, cell counting is done manually, which is known as tedious and time consuming process. Moreover, the manual cell counting can lead inconsistent and imprecise results. Therefore, it is necessary to make an automatic method to detect and count cells from biological images to obtain accurate and consistent results. The proposed multi-step cell counting method automatically segments the cells from the image of cultivated microorganism and labels the cells by utilizing topological analysis of the segmented cells. To improve the accuracy of the cell counting, we adopt watershed algorithm in separating agglomerated cells from each other and morphological operation in enhancing the individual cell object from the image. The system is developed by considering the availability in mobile environments. Therefore, the cell images can be obtained by a mobile phone and the processed statistical data of microorganism can be delivered by mobile devices in ubiquitous smart space. From the experiments, by comparing the results between manual and the proposed automatic cell counting we can prove the efficiency of the developed system.

Performance Optimization Strategies for Fully Utilizing Apache Spark (아파치 스파크 활용 극대화를 위한 성능 최적화 기법)

  • Myung, Rohyoung;Yu, Heonchang;Choi, Sukyong
    • KIPS Transactions on Computer and Communication Systems
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    • v.7 no.1
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    • pp.9-18
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    • 2018
  • Enhancing performance of big data analytics in distributed environment has been issued because most of the big data related applications such as machine learning techniques and streaming services generally utilize distributed computing frameworks. Thus, optimizing performance of those applications at Spark has been actively researched. Since optimizing performance of the applications at distributed environment is challenging because it not only needs optimizing the applications themselves but also requires tuning of the distributed system configuration parameters. Although prior researches made a huge effort to improve execution performance, most of them only focused on one of three performance optimization aspect: application design, system tuning, hardware utilization. Thus, they couldn't handle an orchestration of those aspects. In this paper, we deeply analyze and model the application processing procedure of the Spark. Through the analyzed results, we propose performance optimization schemes for each step of the procedure: inner stage and outer stage. We also propose appropriate partitioning mechanism by analyzing relationship between partitioning parallelism and performance of the applications. We applied those three performance optimization schemes to WordCount, Pagerank, and Kmeans which are basic big data analytics and found nearly 50% performance improvement when all of those schemes are applied.

Estimating an Optimal Scale of a Railway Station with Non-Passengers (철도 비승차 이용객을 고려한 역사 시설물별 적정규모 산정방안)

  • Oh, Tae ho;Lee, Seon ha;Kang, Hee up;Insigne, Maria Sharlene L.;Lee, Sang Jae
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.16 no.4
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    • pp.76-91
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    • 2017
  • The Area of a domestic railway station is designed based on the 4-step traffic demand forecasting model with the average daily passenger count as one of its parameter. However, nowadays, due to increasing rate of railway station's function, the non-passengers are increasing. In order to consider those non-passengers who aren't using trains, assumed volume are added to the average daily passenger count of station to estimate the area, but the criteria being applied has no concrete basis. Therefore, this study aimed to recalculate the increasing non-passenger rate based on actual survey data of station users in any type of railway station to obtain the optimum area. Subsequently, the the design area was performed through pedestrian simulation. According to the result of the simulation, it was found that the total space of the exciting railway stations can be reduced up to 45% and will still satisfy the level of service(LOS) requirement.

Clinical Significance of Argyrophilic Nucleolar Organizer Regions(AgNORs) In Squamous Cell Carcinoma of the Lung (편평세포폐암에서 Argyrophilic Nucleolar Organizer Regions(AgNORs)의 임상적의의)

  • Han, Seung-Beom;Jeon, Young-June;Lee, Sang-Sook
    • Tuberculosis and Respiratory Diseases
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    • v.42 no.4
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    • pp.513-521
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    • 1995
  • Background: Nucleolar organizer regions(NORs) are chromosomal segments encoding for ribosomal RNA and associated with argyrophilic nonhistone protein. Ribosomal RNA genes ultimately direct ribosome and protein synthesis, and it has been suggested the numbers of NORs detected in the cell may reflect nuclear and cellular activity. This study was performed to evaluate the applicability of AgNORs to the diagnosis of squamous cell carcinoma of the lung. Method: The one step silver methods(AgNORs) was used to stain NORs in the routinely processed, formalin fixed, paraffin embedded sections of 36 cases of squamous cell carcinoma of the lung obtained by surgical resection of primary tumor. In each specimen, 100 tumor cells and 100 normal cells adjacent to the tumor chosen at random were examined under an oil immersion lens at a magnification of ${\times}1000$. The mean number of AgNORs per nucleus was calculated for each specimen. Results: The mean number of AgNORs per nucleus(mAgNORs) of normal bronchial epithelium and squamous cell carcinoma of the lung was $1.74{\pm}0.25$ and $4.05{\pm}0.80$, respectively. The difference of mAgNOR between normal and tumor tissue was statistically significant(p<0.001). There was no statistical difference among tumors of different stages. The difference of mAgNOR between normal and tumor tissue was statistically significant in each TNM stage(p<0.05). Conclusion: Mean AgNOR count may be used as a useful marker for the differential diagnosis of benignancy and malignancy, and proliferative activity of the cell in squamous cell carcinoma of the lung. But there was no statistical difference in mean AgNOR count among tumors of different surgical stages. Further studies for the application of mAgNORs to the diagnosis of other histologic types and cytologic specimens of the lung cancer are needed.

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The Detection of Irradiated Composite Seasoning Foods by Analyzing Photostimulated Luminescence (PSL), Electron Spin Resonance (ESR) and Thermoluminescence (TL) (PSL, ESR 및 TL 측정에 의한 복합조미식품의 방사선 조사여부 검지)

  • Kwon Joong-Ho;Kim Mi-Yeung;Kim Byeong-Keun;Chung Hyung-Wook;Kim Tae-Cheol;Kim Soo-Jin
    • Food Science and Preservation
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    • v.13 no.1
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    • pp.55-60
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    • 2006
  • Two kinds of composite seasoning products (beef broth powder, polk bone extract powder) were used for a detection trial of gamma irradiation treatment up to 10 kGy by analyzing photostimulated luminescence (PSL), electron spin resonance (ESR) and thermoluminescence(TL). PSL results showed that the photon counts of non-irradiated samples were lower than 700, while those of irradiated samples were higher than 5000, which makes it possible to screen irradiated composite seasoning products at 1 kGy or over from the non-irradiated control. ESR signals measured for both irradiated samples were not irradiation-specific, even though they were dose dependent in the signal intensity. Radiation-induced TL glow curves were found in irradiated beef broth powder and furthernmore, TL ratio $(TL_4/TL_2)$ obtained by a re-irradiation step could verify the detection result of TL1 glow curves, showing ratios lower than 0.05 in the non-irradiated sample and higher than 1.00 in irradiated ones.

A Study on the Classification of Road Type by Mixture Model (혼합모형을 이용한 도로유형분류에 관한 연구)

  • Lim, Sung Han;Heo, Tae Young;Kim, Hyun Suk
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.28 no.6D
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    • pp.759-766
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    • 2008
  • Road classification system is the first step for determining the road function and design standards. Currently, roads are classified by various indices such as road location and function. In this study, we classify road using various traffic indices as well as to identify traffic characteristics for each type of road. To accomplish the objectives, mixture model was applied for classifying road and analyzing traffic characteristics using traffic data that observed at permanent traffic count stations. A total of 8 variables were applied: annual average daily traffic(AADT), $K_{30}$ coefficient, heavy vehicle proportion, day volume proportion, peak hour volume proportion, sunday coefficient, vacation coefficient, and coefficient of variation(COV). A total of 350 permanent traffic count points were categorized into three groups : Group I (Urban road), Group II (Rural road), and Group III (Recreational road). AADT were 30,000 for urban, 16,000 for rural, and 5,000 for recreational road. Group III was typical recreational road showing higher average daily traffic volume during Sunday and vacational periods. Group I showed AM peak and PM peak, while group II and group III did not show AM peak and PM peak.

Microbiological Evaluation in situ of Each Process in Seed Sprouting (새싹채소 생산현장에서 재배공정별 미생물학적 위해 평가)

  • Jun, So-Yun;Kim, Tae-Hun;Kwon, Joong-Ho;Lee, Yeon-Kyung
    • Food Science and Preservation
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    • v.16 no.6
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    • pp.971-976
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    • 2009
  • The consumption of raw sprouts has increased in popularity worldwide because the food is natural and healthy. However, in Korea, nothing is known on the safety standards of sprout producers or changes in the microbial populations of sprouts during sprouting. We evaluated the microbial safety and quality of sprouts during each step in the sprouting process. Bacteriological analysis showed that seeds had a Total Plate Count (TPC) ranging from 3.04 - 5.21 log CFU/g and coliform counts ranging from 1.80 - 3.86 log CFU/g. TPC and coliform counts increased rapidly during the sprouting process to attain values of 6.99 - 8.26 and 3.70 - 7.15 log CFU/g, respectively, regardless of decontamination of seeds with commercial sanitizer. TPC and coliform counts were on high level after sprouts were washed. Escherichia coli was detected in samples of domestic radish sprouts at all stages from seed to storage, rape sprouts in the stages from soaked seed to storage, and red radish sprouts during sprouting, and no sanitizer was used in any of these processes. Untreated red radish sprouts were also positive for Bacillus cereus at all processing steps and Listeria monocytogenes after germination. However, pathogens were not detected at any sprouting stage of seeds treated with sanitizer. It is necessary to carefully control commercial sprouting, and to develop HACCP guidelines applicable to all sprouting processes, commencing at the first step in raw seed production.

Development of u-Health Care System for Dementia Patients (치매환자를 위한 u-Health Care 시스템 개발)

  • Shin, Dong-Min;Shin, Dong-Il;Shin, Dong-Kyoo
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.38C no.12
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    • pp.1106-1113
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    • 2013
  • For patients who have senile mental disorder such as dementia, quantity of excercise and amount of sunlight are important clue for dose and the treatment. Therefore, monitoring health information of daily life is necessary for patients' safety and healthy life. Portable & wearable sensor device and server configuration monitoring data are needed to provide these services for patients. Watch-type device(smart watch) which patients wear and server system are developed in this paper. Smart watch developed includes GPS, accelerometer and illumination sensor, and can obtain real time health information by measuring the position of patients, quantity of exercise and amount of sunlight. Server system includes the sensor data analysis algorithm and web server that doctor and protector can monitor through sensor data acquired from smart watch. The proposed data analysis algorithm acquires quantity of exercise information and detects step count in patients' motion acquired from acceleration sensor and to verify this, the three cases with fast pace, slow pace, and walking pace show 96% of the experimental result. If developed u-Healthcare System for dementia patients is applied, more high-quality medical service can be provided to patients.

Efficient Pipeline Architecture of CABAC in H.264/AVC (H.264/AVC의 효율적인 파이프라인 구조를 적용한 CABAC 하드웨어 설계)

  • Choi, Jin-Ha;Oh, Myung-Seok;Kim, Jae-Seok
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.45 no.7
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    • pp.61-68
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    • 2008
  • In this paper, we propose an efficient hardware architecture and algorithm to increase an encoding process rate and implement a hardware for CABAC (Context Adaptive Binary Arithmetic Coding) which is used with one of the entropy coding ways for the latest video compression technique, H.264/AVC (Advanced Video Coding). CABAC typically provides a better high compression performance maximum 15% compared with CAVLC. However, the complexity of operation of CABAC is significantly higher than the CAVLC. Because of complicated data dependency during the encoding process, the complexity of operation is higher. Therefore, various architectures were proposed to reduce an amount of operation. However, they have still latency on account of complicated data dependency. The proposed architecture has two techniques to implement efficient pipeline architecture. The one is quick calculation of 7, 8th bits used to calculate a probability is the first step in Binary arithmetic coding. The other is one step reduced pipeline arcbitecture when the type of the encoded symbols is MPS. By adopting these two techniques, the required processing time was reduced about 27-29% compared with previous architectures. It is designed in a hardware description language and total logic gate count is 19K using 0.18um standard cell library.

Exploration of Preservation Hurdles in Korean Traditional Side Dishes (한국전통 밑반찬류에 사용된 보존 Hurdle의 발굴)

  • Chung Sun-Kyung;Lyu Eun-Soon;Lee Dong-Sun
    • Food Science and Preservation
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    • v.13 no.2
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    • pp.259-268
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    • 2006
  • We investigated food preservation hurdles used for Korean traditional side dishes. As a first step of the research preparation and cooking recipes of the side dishes were surveyed, which are commonly used in Korean households. As next step, compositional and microbial quality attributes were measured onto the samples collected from the market. Antimicrobial ingredient added in the preparation are reasoned to work as important hurdles based on the scientific principles. Heating processes such as blanching, boiling, braising, hard-boiling and frying help to keep the produce decontamination or water activity adjustment. Measured salt contents of most side dishes were in the range of 1-5% with those of salt-preserved vegetables being higher. pH values were 4.7-6.4 with those of salt-preserved vegetables being lower and those of hard boiled fishes being higher. Soluble solids of braised or hard-boiled dishes were usually above $40^{\circ}Brix$, while those of blanched and seasoned vegetables had lower values. Water activity values were 0.93-0.95 for seasoned vegetables and 0.77-0.88 for hard-boiled or semi dried beans and seafoods. Product with processing steps of braising or blanching showed lower bacterial load of 102-104 cfu/g, while seasoned or salt-preserved vegetables and seafoods had aerobic bacterial count above 106 cfu/g. Korean traditional side dishes were found to apply the appropriate combinations of heating preparation process, water activity and pH adjustment and salting, providing the required preservation properties.