• Title/Summary/Keyword: stem rot.

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First Report of Stem Rot in Statice Caused by Rhizoctonia solani in Korea (Rhizoctonia solani에 의한 스타티스 줄기썩음병)

  • Kang, Mi-Hyung;Cheong, Dong-Chun;Choi, Chang-Hak;Lim, Hoi-Chun;Song, Young-Ju;Noh, Tae-Hwan;Lee, Du-Ku;Kim, Hyung-Moo
    • Research in Plant Disease
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    • v.15 no.1
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    • pp.54-56
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    • 2009
  • Stem rot of perennial statice (Limonium sinuatum) was observed in Un bong, Jeonbuk from 2006 to 2007. Affected plants were randomly distributed in the greenhouses and infection rate was more than 10%. Stem and leaf of statice at soil line were dried and turned brown, initially. As the disease became severe, other stem parts and crowns were turned dark brown and then sunken. The fungal isolates were showed initially white aerial mycelium and turned brown with age. They produced few sclerotia which small, irregularly shaped with pinpoint sized. Mycelia were branched at $90^{\circ}$ angles and multinucleate in one cell. The pathogenicity of causal organism was proved according to Koch's postulates. The causal fungus of stem rot was identified as Rhizoctonia solani based on the cultural and morphological characteristics. This is the first report on stem rot of statice by R. solani in Korea.

Stem Rot of Stachys sieboldii Caused by Sclerotium rolfsii in Korea (Sclerotium rolfsii에 의한 초석잠 흰비단병)

  • Kwon, Jin-Hyeuk;Kang, Dong-Wan;Lee, Seong-Tae;Choi, Ok-Hee;Shen, Shun-Shan
    • Research in Plant Disease
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    • v.17 no.3
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    • pp.399-401
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    • 2011
  • Stem rot of Stachys sieboldii occurred sporadically in the farmer's fields at Shindeung-myeon, Sancheonggun, Gyeongnam province in Korea. The infected leaves and stems are water-soaked, rotted, wilted and finally leads to the death of the whole plants. The sclerotia, 1-3 mm, white to brown, globoid, were formed on lesions and the surface of soil line. The optimum temperature for mycelial growth and sclerotia formation was $30^{\circ}C$ on potato dextrose agar. The typical clamp connections were observed in the hyphae of the fungus. On the basis of mycological characteristics and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report of stem rot on S. sieboldii caused by S. rolfsii in Korea.

Screening and Identification of Antifungal Bacillus sp. #72 against the Pathogenic Stem-end Rot of Kiwi Fruit (참다래 꼭지썩음병을 일으키는 Diaporthe actinidiae을 억제하는 길항성 Bacillus sp. #72의 분리 및 동정)

  • Cho, Jung-Il;Cho, Ja-Yong;Park, Yong-Seo;Yang, Seung-Yul;Heo, Buk-Gu
    • The Korean Journal of Community Living Science
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    • v.18 no.2
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    • pp.241-246
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    • 2007
  • This study was conducted to select and screen for an antifungal bacterial strain showing pathogen inhibitory activity against Diaporthe actinidiae, which causes stem-end rot in kiwi fruit. Four bacterial strains were isolated which strongly inhibit Diaporthe actinidiae from among two hundred and fifty bacterial strains screened from the soil where kiwi fruit were grown. By co-culturing bacterial strain #72 and the pathogen causing the stem-end rot of kiwi fruit, bacterial strain #72 showed 81.0% antifungal activity against Diaporthe actinidiae. Bacterial strain #72 was identified to be from the genus Bacillus sp. based on morphological and biochemical characterization. The bacterialization of culture broth for Bacillus sp. #72 which was sterilized at $121^{\circ}C$ for 15 minutes and than purified by $0.45{\mu}m$ membrane filter showed almost all of the antagonistic activity against Diaporthe actinidiae. We have also confirmed that in vitro treatment of Bacillus sp. #72 cultured in SD+B+P(sugar 5%, soy sauce 3%, beef extract 0.2%, peptone 0.2%) medium efficiently inhibited the growth of Diaporthe actinidiae responsible for stem-end rot in kiwi fruit.

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Stem Rot of English Ivy Caused by Phytophthora cinnamomi (Phytophthora cinnamomi에 의한 아이비줄기썩음병)

  • Kim, Hyung-Moo;Jin, Kyo-Hoon;Ko, Jung-Ae
    • Research in Plant Disease
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    • v.13 no.3
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    • pp.207-210
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    • 2007
  • A stem and root rot disease of English ivy (Hedera helix) was found in the flower market, Jeonju, Chonbuk province, Korea in May 2007. A Phytophthora species was isolated from the diseased part of the plant. Based on the mycological properties and pathogenesis the isolate was identified as Phytophthora cinnamomi. The fungus produce ovoid sporangium which was ovoid to spherical and obpyriform which is noncaducous, non-papillate and averaged $33.8{\times}16{\mu}m$ in dimension. Chlamydospores were abundantly produced on agar media and sized about $19.7{\mu}m$ in diameter. The fungus was heterothallic and A1 mating type. Oospores were measured $26.2{\mu}m$ in size. Optimum temperature for growth of the fungus was 25 to $30^{\circ}C$. Up to our knowledge, this is the first report demonstrating the stem rot on English ivy caused by P. cinnamomi in Korea.

Stem Rot of English Ivy Caused by Phytophthora nicotianae (Phytophthora nicotianae에 의한 아이비줄기썩음병)

  • Kim, Hyung-Moo;Jin, Kyo-Hoon;Ko, Jung-Ae
    • Research in Plant Disease
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    • v.14 no.2
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    • pp.122-126
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    • 2008
  • A stem and leaf rot disease of English ivy(Hedera helix) was found in the flower market, Jeonju, Chonbuk province, Korea in July 2007. A Phytophthora species was isolated from the diseased part of the plant. Based on the mycological properties and pathogenesis the isolate was identified as Phytophthora nicotianae. The fungus produce ovoid sporangium which was ovoid to spherical which is noncaducous, papillate and averaged $35.4{\times}25.2{\mu}m$ in dimension. Chlamydospores were abundantly produced on agar media and sized about 28.5 ${\mu}m$ in diameter. The fungus was heterothallic and A1 mating type. Oospores were measured 23.3 ${\mu}m$ in size. Optimum temperature for growth of the fungus was 25 to $30^{\circ}C$. Up to our knowledge, this is the first report demonstrating the stem rot on English ivy caused by P. nicotianae in Korea.

Stem Rot of Kalanchoe Caused by Phytophthora nicotianae (Phytophthora micotianae 에 의한 칼랑코에 역병)

  • 한경숙;이중섭;지형진
    • Research in Plant Disease
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    • v.7 no.1
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    • pp.8-10
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    • 2001
  • A stem and root rot disease of kalanchoe (Kalanchoe sp.) which is a succulent plant that provides consumers with a durable flowering pot was found in Koyang, Kyounggi province, Korea in May 1998. We found that stems and roots of potted kalanchoe had dark brown spots at the soil level. The causal organism was identified as Pjytophtora nicotianae on the basis of mycological characteristics. The fungus produced markedly papillate, ovoid to spherical sporangia, and abundant chlamydospores. Sporangia were 20∼48$\times$24∼64㎛(avg. 35.0$\times$47.3㎛) in size, and optimum temperature for the mycelial growth of the isolate was 30$\^{C}$. The fungus showed relatively different pathogenicity to 14 kalanchoe cultivars including K. blossfelana cv. Florus. cultivars florsu, calypso, Maya, and Redsing were susceptible to thedisease in root dip inoculation. This is the first report demonstrating the stem rot on kalanchoe caused by P. nicotianae in Korea.

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Effect of Potassium Phosphonate on the Control of Potato Basal Stem Rot(Pythium myriotylum) in Hydroponics (아인산염을 이용한 감자 줄기기부썩음병 방제)

  • Hong, Soon-Yeong;Kang, Hyeong-Sik;Kang, Yong-Kil;Jee, Hyeong-Jin
    • Research in Plant Disease
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    • v.10 no.2
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    • pp.142-144
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    • 2004
  • Effect of potassium phosphonate on control of potato basal stem rot caused by Pythium myriotylum in hydroponics system was evaluated at spring and fall cultivation in Jeju island in 2001. Potassium phosphonate 200 ppm treatment, which was directly supplemented into the nutrient solution, showed 90.5%, and 100 ppm treatment showed 50.5% control value in spring cultivation. Fall cultivation, potassium phosphonate 200 ppm treatment showed 100%, and 100 ppm treatment showed 69.9% control value. Potassium phosphonate 200 ppm treatment, basal stem rot of potato started to develop 10 days ofter inoculation into the hydroponics system showed 8.0% in infection rate. Potassium phosphonate 100 ppm treatment, after 5 days showed 10.0%, and after 10 days showed 20.0% infection rate, but the non-treated was after 5 days showed 56.7%, and after 10 days showed 96.9% infection rate.

Stem Rot of Perilla Caused by Sclerotium rolfsii in Korea (Sclerotium rolfsii에 의한 들깨 흰비단병)

  • Kwon, Jin-Hyeuk;Choi, Si-Lim;Lee, Heung-Su;Shim, Hong-Sik
    • The Korean Journal of Mycology
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    • v.40 no.3
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    • pp.177-178
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    • 2012
  • Stem rot of perilla (Perilla frutescens var. japonica Hara) occurred sporadically in the exhibition field at Gyeongsangnam-do Agricultural Research and Extension Services in Korea. The infected stems are water-soaked, rotted, wilted and finally leads to the death of the whole plants. The sclerotia, 1-3 mm, white to brown, spherical, were formed on lesions and the surface of soil line. The optimum temperature for mycelial growth and sclerotia formation was $30^{\circ}C$ on potato dextrose agar and the hyphal width was 4-9 ${\mu}m$. The typical clamp connections were observed in the hyphae of the fungus. On the basis of mycological characteristics and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report of stem rot on perilla caused by S. rolfsii in Korea.

Occurrence of Stem Canker and Tuber Rot on Yam Caused by Rhizoctonia solani AG 2-2IIIB in Korea (Rhizoctonia solani AG 2-2IIIB에 의한 마 뿌리썩음병의 한국 내 발생)

  • Hong, Sung Kee;Lee, Jae Kook;Lee, Young Kee;Lee, Sang Yeob;Kim, Wan Gyu;Shim, Hong Sik
    • The Korean Journal of Mycology
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    • v.40 no.4
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    • pp.266-270
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    • 2012
  • Stem canker and tuber rot symptoms were observed on yam grown in Andong and Jinju, Korea in 2011. A total of 20 isolates of Rhizoctonia and allied fungi were obtained from the symptomatic plants. Among the isolates, 8 isolates were identified as Rhizoctonia solani and 12 isolates as Ceratobasidium sp. based on rDNA-internal transcribed spacer (ITS) sequence similarity. In the cluster analysis of rDNA-ITS sequences, 7 isolates of R. solani belonged to AG 2-2IIIB and remaining one to AG 1-1A. In addition, among the 12 isolates of Ceratobasidium sp., 7 isolates belonged to AG-Fa, three isolates to AG-A and the other two isolates to AG-Fb and AG-O, respectively. Pathogenicity tests showed that all the R. solani AG 2-2IIIB isolates are pathogenic on stem and tuber of yam but R. solani AG 1-1A and all the Ceratobasidium isolates are non-pathogenic. The results indicate that R. solani AG 2-2IIIB is an important pathogen causing stem canker and tuber rot on yams grown in the study areas. This is the first report of R. solani AG 2-2IIIB causing stem canker and tuber rot of yam in Korea.

First Report of Macrophomina phaseolina Causing Charcoal Rot in Bottle Gourd in Korea

  • Kim, Sang Gyu;Kim, Tae Bok;Lee, Oak Jin
    • The Korean Journal of Mycology
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    • v.49 no.3
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    • pp.399-403
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    • 2021
  • Stem blight symptoms were observed in greenhouse-grown bottle gourds (Lagenaria siceraria) in Wanju, Korea. Infected plants showed leaf chlorosis and wilting; the plants eventually died with the infected leaves remaining attached, and black sclerotia formed on the diseased stem. Based on the morphological characteristics and pathogenicity results and sequence analyses of the internal transcribed spacer, translation elongation factor 1-α, β-tubulin, and calmodulin regions, the isolated fungus was Macrophomina phaseolina. This is the first report of charcoal rot in Lagenaria siceraria caused by Macrophomina phaseolina in Korea.