• 한국발생생물학회지:발생과생식
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• 제21권4호
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• pp.425-434
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• 2017

Polyploidy is occurred by the process of endomitosis or cell fusion and usually represent terminally differentiated stage. Their effects on the developmental process were mainly investigated in the amphibian and fishes, and only observed in some rodents as mammalian model. Recently, we have established tetraploidy somatic cell nuclear transfer-derived human embryonic stem cells (SCNT-hESCs) and examined whether it could be available as a research model for the polyploidy cells existed in the human tissues. Two tetraploid hESC lines were artificially acquired by reintroduction of remained 1st polar body during the establishment of SCNT-hESC using MII oocytes obtained from female donors and dermal fibroblasts (DFB) from a 35-year-old adult male. These tetraploid SCNT-hESC lines (CHA-NT1 and CHA-NT3) were identified by the cytogenetic genotyping (91, XXXY,-6, t[2:6] / 92,XXXY,-12,+20) and have shown of indefinite proliferation, but slow speed when compared to euploid SCNT-hESCs. Using the eight Short Tendem Repeat (STR) markers, it was confirmed that both CHA-NT1 and CHA-NT3 lines contain both nuclear and oocyte donor genotypes. These hESCs expressed pluripotency markers and their embryoid bodies (EB) also expressed markers of the three embryonic germ layers and formed teratoma after transplantation into immune deficient mice. This study showed that tetraploidy does not affect the activities of proliferation and differentiation in SCNT-hESC. Therefore, tetraploid hESC lines established after SCNT procedure could be differentiated into various types of cells and could be an useful model for the study of the polyploidy cells in the tissues.

• 한국환경과학회지
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• 제31권11호
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• pp.941-950
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• 2022

This study was conducted to provide data and stem information to establish a local volume table of Cryptomeria japonica in Jeju Island. Stem analysis was performed on 26 trees by selecting two average trees from each site of the 13 plots of C. japonica stands in 2021 and 2022. During the analysis stage, one outlier tree was rejected, and a total of 260 observations of the specific stem height of 25 trees were used. Of the seven major taper equation models applied for parameter estimation and statistical verification, the Muhairwe 1999 model was found to be the best fit and selected as the optimal model. Stem shape-related estimates were acquired through the selected model, and sectional measurements according to the Smalian formula applied at an interval of 10 cm from the height of the stem were used to develop a volume table. A paired t-test comparison between the C. japonica volume obtained from the present study and those selected from the current yield table by NIFoS(2020), revealed significant differences (p<0.05), highlighting the necessity of a local volume table for C. japonica in Jeju Island.

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2935-2941
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• 2012

Biological response modifiers (BRMs) can alter interactions between the immune system and cancer cells to boost, direct, or restore the body's ability to fight disease. Mice with ethylnitrosourea- (ENU) induced leukemia were here used to monitor the therapeutic efficacy of lipopolysaccaride (LPS), Bacillus Calmette Guerin (BCG) and sheep erythrocytes (SRBC). Flow cytometry based CD34+ positivity analysis, clonogenicity, proliferation and ultrastructure studies using scanning electron microscopy (SEM) of stem cells in ENU induced animals with and without BRMs treatment were performed. BRMs improved the stem-stromal relationship structurally and functionally and might have potential for use as an adjunct in human stem cell therapy.

• 대한음성학회지:말소리
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• 제54호
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• pp.45-61
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• 2005

In this paper, we present a method of generating intonation contour for Korean text-to-speech (TTS) system and a method of synthesizing emotional speech, both based on Soft template mark-up language (Stem-ML), a novel prosody generation model combining mark-up tags and pitch generation in one. The evaluation shows that the intonation contour generated by Stem-ML is better than that by our previous work. It is also found that Stem-ML is a useful tool for generating emotional speech, by controling limited number of tags. Large-size emotional speech database is crucial for more extensive evaluation.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1988년도 한국자동제어학술회의논문집(국제학술편); 한국전력공사연수원, 서울; 21-22 Oct. 1988
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• pp.777-781
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• 1988

A method for weighing fruits without separating them from stem is proposed. The base of stem is fixed and a fruit or a cluster of fruits is forced to vibrate. The approximated vibration model is constructed by the use of Transfer Matrix Method. The natural frequency (w) in this model can be represented as a function of weight elements, and the length and stiffness of branch elements of stem. With this function, only w is possible to measure. However, several small weights whose weights are known are attached to weight elements in various combinations. From these equations, unknown parameters are determined so that the weight of each fruit can be obtained by a non-destructive method.

• 한국산림과학회지
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• 제98권5호
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• pp.576-583
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• 2009

Based on the stem analysis and biomass measurement of 36 trees and 1,576 branches in Pinus sylvestris var. mongolica (Mongolian pine) plantations of Northeast China, this study was conducted to develop estimation model equation for leaf biomass of a single tree and branch, to examine the vertical distribution of leaf biomass in the crown, and to evaluate the proportional ratios of biomass by tree parts, stem, branch, and leaf. The results indicated that DBH and crown length were quite appropriate to estimate leaf biomass. The biomass of single branch was highly correlated with branch collar diameter and relative height of branch in the crown, but not much with stand density, site quality, and tree height. Weibull distribution function would have been appropriate to express vertical distribution of leaf biomass. The shape parameters from 29 sample trees out of 36 were less than 3.6, indicating that vertical distribution of leaf biomass in the crown was displayed by bell-shaped curve, a little inclined toward positive side. Apparent correlationship was obtained between leaf biomass and branch biomass having resulted in linear function equation. The stem biomass occupied around 80% and branch and leaf made up about 20% of total biomass in a single tree. As the level of tree class was increased from class I to class V, the proportion of the stem biomass to total biomass was gradually increased, but that of branch and leaf became decreased.

• The Korean Journal of Physiology and Pharmacology
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• 제21권2호
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• pp.153-160
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• 2017

In this study, we aim to determine the in vivo effect of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs) on neuropathic pain, using three, principal peripheral neuropathic pain models. Four weeks after hUCB-MSC transplantation, we observed significant antinociceptive effect in hUCB-MSC-transplanted rats compared to that in the vehicle-treated control. Spinal cord cells positive for c-fos, CGRP, p-ERK, p-p 38, MMP-9 and MMP 2 were significantly decreased in only CCI model of hUCB-MSCs-grafted rats, while spinal cord cells positive for CGRP, p-ERK and MMP-2 significantly decreased in SNL model of hUCB-MSCs-grafted rats and spinal cord cells positive for CGRP and MMP-2 significantly decreased in SNI model of hUCB-MSCs-grafted rats, compared to the control 4 weeks or 8weeks after transplantation (p<0.05). However, cells positive for TIMP-2, an endogenous tissue inhibitor of MMP-2, were significantly increased in SNL and SNI models of hUCB-MSCs-grafted rats. Taken together, subcutaneous injection of hUCB-MSCs may have an antinociceptive effect via modulation of pain signaling during pain signal processing within the nervous system, especially for CCI model. Thus, subcutaneous administration of hUCB-MSCs might be beneficial for improving those patients suffering from neuropathic pain by decreasing neuropathic pain activation factors, while increasing neuropathic pain inhibition factor.

• 한국동물생명공학회지
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• 제34권3호
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• pp.139-147
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• 2019

Stem cells are progenitor cells that are capable of self-renewal and differentiation into various cells. Especially, pluripotent stem cells (PSCs) have in vivo and in vitro differentiation capacity into three germ layers and can proliferate infinitely. The differentiation ability of PSCs can be applied for regenerative medicine and tissue engineering. In domestic animals, their PSCs have a potential for preclinical therapy as well as the production of transgenic animals and agricultural usage such as cultured meat. Among several domestic animals, a pig is considered as an ideal model for biomedical and agricultural purposes mentioned above. In this reason, studies for pig PSCs including embryonic stem cells (ESCs), embryonic germ cells (EGCs) and induced pluripotent stem cells (iPSCs) have been conducted for decades. Therefore, this review will discuss the history of PSCs derived from various origins and recent progress in pig PSC research field.

• 한국산림과학회지
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• 제112권4호
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• pp.417-425
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• 2023

본 연구는 난대상록수종인 붉가시나무에 대한 수간곡선을 도출하고 이를 이용하여 수간재적표를 작성하기 위함이 그 목적이다. 분석에 사용된 공시목은 전남, 경남, 제주 등에서 수집된 688본이며, 수간곡선 도출을 위하여 적용한 수간곡선 모형은 Max & Burkhart 모델, Kozak 모델 및 Lee 모델 등이다. 3가지 모델 중 우리나라 붉가시나무 수간형태를 가장 잘 설명하는 것은 Kozak 모델인 것으로 분석되었다. 이 모델의 적합도는 0.9583, 편의는 0.0352, 추정치 표준오차의 백분율은 1.1439, 평균절대편차는 0.6751로 각각 나타났다. 붉가시나무의 수간재적표는 수간곡선을 설명하는데 최적인 것으로 나타난 Kozak 모델을 이용하였으며, 재적 계산은 수간고별 직경으로 Smalian 식을 적용시켜 산출하였다. 그리고 이전에 만들어진 붉가시나무 수간재적표 2가지(2007년, 2010년)와 이번에 만든 재적표(2023년) 간을 서로 비교하기 위해 분산분석을 실시하였다. 그 결과 2007년 완도지역에서 만든 재적표가 2010년과 2023년에 만든 재적표 보다 약 2배가 많은 값을 가지는 것으로 나타났다. 이번에 작성된 수간재적표(2023년)는 공시재료의 지역별 수집범위와 공시목이 많을 뿐만 아니라, 충분한 과학적 기반 하에서 만들어졌다. 따라서 전국단위의 공식적인 붉가시나무 수간재적표로 사용될 수 있을 것으로 판단된다.

• Journal of Veterinary Science
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• 제22권1호
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• pp.7.1-7.13
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• 2021

Background: Niemann-Pick disease type C (NPC) is caused by the mutation of NPC genes, which leads to the abnormal accumulation of unesterified cholesterol and glycolipids in lysosomes. This autosomal recessive disease is characterized by liver dysfunction, hepatosplenomegaly, and progressive neurodegeneration. Recently, the application of induced neural stem cells (iNSCs), converted from fibroblasts using specific transcription factors, to repair degenerated lesions has been considered a novel therapy. Objectives: The therapeutic effects on NPC by human iNSCs generated by our research group have not yet been studied in vivo; in this study, we investigate those effects. Methods: We used an NPC mouse model to efficiently evaluate the therapeutic effect of iNSCs, because neurodegeneration progress is rapid in NPC. In addition, application of human iNSCs from NPC patient-derived fibroblasts in an NPC model in vivo can give insight into the clinical usefulness of iNSC treatment. The iNSCs, generated from NPC patientderived fibroblasts using the SOX2 and HMGA2 reprogramming factors, were transplanted by intracerebral injection into NPC mice. Results: Transplantation of iNSCs showed positive results in survival and body weight change in vivo. Additionally, iNSC-treated mice showed improved learning and memory in behavior test results. Furthermore, through magnetic resonance imaging and histopathological assessments, we observed delayed neurodegeneration in NPC mouse brains. Conclusions: iNSCs converted from patient-derived fibroblasts can become another choice of treatment for neurodegenerative diseases such as NPC.