• Mycobiology
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• 제40권1호
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• pp.59-65
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• 2012

Antagonistic microorganisms against $Rhizoctonia$ $solani$ were isolated and their antifungal activities were investigated. Two hundred sixteen bacterial isolates were isolated from various soil samples and 19 isolates were found to antagonize the selected plant pathogenic fungi with varying degrees. Among them, isolate C9 was selected as an antagonistic microorganism with potential for use in further studies. Treatment with the selected isolate C9 resulted in significantly reduced incidence of stem-segment colonization by $R.$ $solani$ AG2-2(IV) in Zoysia grass and enhanced growth of grass. Through its biochemical, physiological, and 16S rDNA characteristics, the selected bacterium was identified as $Bacillus$ $subtilis$ subsp. $subtilis$. Mannitol (1%) and soytone (1%) were found to be the best carbon and nitrogen sources, respectively, for use in antibiotic production. An antibiotic compound, designated as DG4, was separated and purified from ethyl acetate extract of the culture broth of isolate C9. On the basis of spectral data, including proton nuclear magneric resonance ($^1H$ NMR), carbon nuclear magneric resonance ($^{13}C$ NMR), and mass analyses, its chemical structure was established as a stereoisomer of acetylbutanediol. Application of the ethyl acetate extract of isolate C9 to several plant pathogens resulted in dose-dependent inhibition. Treatment with the purified compound (an isomer of acetylbuanediol) resulted in significantly inhibited growth of tested pathogens. The cell free culture supernatant of isolate C9 showed a chitinase effect on chitin medium. Results from the present study demonstrated the significant potential of the purified compound from isolate C9 for use as a biocontrol agent as well as a plant growth promoter with the ability to trigger induced systemic resistance of plants.

• 한국식품영양학회지
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• 제30권6호
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• pp.1191-1198
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• 2017

This study aimed to evaluate the utilization of the top of a low intake of root, through the analysis of the antioxidant activity of the powder of hydroponic-cultured ginseng. Quality characteristics and antioxidant activity were compared and analyzed with Korea's traditional dessert Gaeseong-Juak, which is made of the powder of hydroponic-cultured ginseng's leaf by adjusting the added volume. DPPH radical scavenging activity of the hydroponic-cultured ginseng by the part powder was in the following order: highly stem, leaf and root. It was measured as 67.9%, 42.9%, and 25.9% at the 1 mg/m level. Gaeseong-Juak was prepared by adding hydroponic-cultured ginseng leaf powder at 0%, 0.3%, 0.6%, and 0.9% of the rice powder. As the content of hydroponic-cultured ginseng leaf powder increased, moisture content, L-value, and a-value were significantly decreased, while the b-value was significantly increased. The texture profile analysis of Gaeseong-Juak was not significantly different among the samples. In the sensory test, the sample containing 0.6% hydroponic-cultured ginseng leaf powder achieved good scores. The DPPH radical scavenging activity of Gaeseong-Juak was significantly increased, as the addition level increased, compared to the original. Based on the above results, hydroponic-cultured ginseng leaf was verified to be a possible natural antioxidant. It can increase food's nutritional values and possibilities when made of hydroponic-cultured ginseng, using leaf which is added to the traditional dessert Gaeseong-Juak.

• Journal of Plant Biotechnology
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• 제31권1호
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• pp.37-41
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• 2004

백리향(Thymus quinquecostatus Celak.)의 기내번식 방법을 개발하기 위해 줄기증식에 미치는 싸이토키닌 효과를 조사하였다. 시료식물은 MS배지에 thidiazuron등 5종의 싸이토키닌을 단독 또는 혼합 처리하여 광배양 또는 암배양 조건에서 식물체 증식을 유도하였다. 백리항의 대량증식에 효과적인 cytokinin종류와 농도는 명배양시 줄기수에 있어서 BA 1.0＋TDZ 0.1mg/L (6.50$\pm$1.51) 였고, 신초의 길이는 zeatin 2.0＋TDZ 0.1mg/L(3.27$\pm$0.7)가 최적 조건이었다. 또한 암배양시 줄기수의 경우엔 처리간에 별다른 차이 없이 비슷한 경향을 나타낸 가운데 2-ip 2.0mg/L로 처리하였을 때가 가장 뛰어났고, 줄기의 신장은 zeatin 2.0mg/L일 때가 가장 효과적이었다. 전체적으로 기내에서 대량증식율은 평균 89.58%로 비교적 대부분의 시료에서 식물체가 유도되었다.

• Journal of Plant Biotechnology
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• 제36권2호
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• pp.124-129
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• 2009

In general, the root color of Codonopsis lanceolata is white, but red or blue-colored root is found at a low frequency in nature. Red or blue-colored roots have scarcity value, thus farmers wish to produce colored roots. The factors for determining the color of roots are unclear whether the color is controlled by genetically or simply by environmentally such as soil environment. Using in vitro culture system which is advantageous for setting of the same culture condition, we analyzed the physiological and morphological characteristics and genetic differences among red-, blue- and white lines of C. lanceolata. In the red colored roots, stems of in vitro cultured plantlet were colored in dark red pigment. Histological analysis revealed that the red pigment was accumulated in the outer cortex layer of the stem and determined as anthocyanin. Chlorophyll contents in red root lines were higher than those in white- and blue root lines. Plantlets from red roots were smaller in both shoot length and total leaf area than those from white- and blue roots. Genetic differences among the three different colored C. lanceolata were determined by RAPD (Randomly Amplified Polymorphic DNA) analysis. Each line of colored roots had clear DNA polymorphism. These results indicate that the occurrence of red- and blue colored roots in nature was determined by genetic factors rather than soil enviromental conditions.

• Journal of Plant Biotechnology
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• 제36권2호
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• pp.163-169
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• 2009

Dendropanax morbifera (Araliaceae) is an endemic species in Korea and distributed in the southern part of Korea. The roots and stems of this plant have been used for folk medicine for the treatment of migraine headache, dysmenorrheal, and remove wind dampness and for Vanishes production. Production of adventitious roots in D. morbifera by in vitro cultures could be used as alternatives materials. Leaf, stem, and root segments from D. morbifera seedling were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg/L IBA and 30 g/L sucrose. After 4 weeks of culture, the highest induction of adventitious roots was obtained from the leaf segment. Frequency of adventitious root formation on medium with various kinds of auxins (IAA, NAA, 2,4-D, and IBA) and various concentrations of IBA (0, 0.1, 0.5, 1.0, 3.0, and 5.0 mg/L) was tested. The maximum induction of adventitious root was obtained on medium with 1.0 mg/L IBA. In liquid culture, growth of root was best 1/2MS medium supplemented with 1.0 mg/L IBA and 30 g/L sucrose. Adventitious roots were cultured in 5 L bioreactor containing 1/2 MS medium supplemented with 1.0 mg/L IBA and 30 g/L sucrose and mass-production of adventitious roots was successfully achieved. This study demonstrated for the first time to produce adventitious roots in D. morbifera.

• 원예과학기술지
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• 제30권3호
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• pp.325-328
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• 2012

'My Sun'은 경기도농업기술원에서 2009년도에 육성한 분화용 국화 신품종으로 2005년도에 오렌지 계열 분화용 국화 'Omega Time Orange' 품종을 모본으로 하고 흰색 'Tasman' 품종을 부본으로 교배하여 얻었다. 이 품종은 노란색 꽃잎을 가진 초장이 짧은 홑꽃이다. 2006-2009년까지 춘계 촉성재배와 동계 억제재배를 포함하는 특성검정을 수행하였다. 'My Sun' 품종의 자연개화기는 10월 13일이며, 전조재배와 차광재배로 개화가 조절되어 주년 재배가 가능하다. 화경은 21mm, 착화수는 본당 34.4매이고 꽃잎수는 20.4매이다. 엽색은 녹색(Green Group 137A)이고 초장은 13.3cm이다. 개화소요일수는 춘계에는 단일처리 후 42일 정도이며, 분지수는 겨울철에는 3.4개 정도를 가진다. 이 품종은 흰녹병 저항성이고 대조품종에 비해 기호도가 높은 편이었다.

• Archives of Plastic Surgery
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• 제33권1호
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• pp.1-4
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• 2006

Cryopreserved fibroblast implants represent a major advancement for healing of chronic wounds. Bone marrow stromal cells, which include the mesenchymal stem cells, have a low immunity-assisted rejection and are capable of expanding profoundly in a culture media. Therefore, they have several advantages over fibroblasts in clinical use. The ultimate goal of this study was to compare the wound healing accelerating growth factor secretion of the bone marrow stromal cells with that of the fibroblasts and this pilot study particularly focuses on the growth factor secretion to accelerate wound healing. Bone marrow stromal cells and fibroblasts were isolated from the same patients and grown in culture. At 1, 3, and 5 days post-incubating, secretion of basic fibroblast growth factor(bFGF), vascular endothelial growth factor (VEGF), and transforming growth factor beta(TGF-${\beta}$) were compared. In TGF-${\beta}$ secretion fibroblasts showed 12~21% superior results than bone marrow stromal cells. In contrast, bFGF levels in the bone marrow stromal cells were 47~89% greater than that in fibroblasts. The VEGF levels of the bone marrow stromal cells was 7~12 fold greater than that of the fibroblasts. Our results suggest that the bone marrow stromal cells have great potential for wound healing accelerating growth factor secretion.

• 한국식생활문화학회지
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• 제13권5호
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• pp.519-528
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• 1998

This study was conducted to investigate the changes in lipid components of red pepper under various processing conditions such as drying, exposing to lights, extraction and storage conditions. The material used in this study were Chungyang cultivars which contained the largest amount of capsaicinoids and Dabok cultivars which is most popular among the various cultivars produced in Korea. Total lipid content of Chungyang was 15.7% and those of Dabok, Udeungsang and Hongilpum were about 17%. The neutral lipid content in free lipid of red pepper was $78.5{\sim}80.3%$ Phospholipid and glycolipid content in bound lipid were $53.8{\sim}56.9%$ and $35.3{\sim}38.7%$ respectively. The major fatty acids of lipid were linoleic, palmitic and oleic acid. Linoleic acid was presented mainly in pericarp, seed and placenta, whereas the most of palmitic acid was presented in stem. Lipid content of cut and whole red pepper were decreased $24.7{\sim}28%$ and $18.1{\sim}21.5%$ by drying for 48hrs at $65^{\circ}C$ and $95^{\circ}C$. And lipid content was also decreased $3.5{\sim}3.6%$ when the red pepper powder was exposed to 15,000 lux of incandescent light for 30 days at $40^{\circ}C$ and $4{\sim}4.9%$ to fluorescent light. The lipid was extracted the highest content of $50.5{\sim}51.7%$ by acid solution(pH2) and the lowest content by neutral solution, and the higher the salt concentration, the greater the lipid was extracted. During storage at $4^{\circ}C,\;25^{\circ}C,\;40^{\circ}C$, for 30 days the lipid content was not much changed but linoleic acid content was decreased during storage at the same conditions.

• 한국실내디자인학회논문집
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• 제17권2호
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• pp.3-12
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• 2008

Most of these cultural spaces are Western architecture which is completely unrelated to our traditional style. In Japan's colonial rule of Korea these Western formation flowed in and passed on by them. Therefore before the understanding of modem cultural space built in Japan's colonial rule of Korea, it's essential to study about the background of the western period in that time, architecture and trend of interior. Due to Great Depression and other reasons the economical modernism of architecture raised in this period. In this thesis, based on such background of period, cultural space has been divided into assembly, theatre and exhibition spaces. Those spaces were studied according to its expressional characteristics and brought to following conclusion from the analysis. The cultural spaces were created around 1930 due to an increase of demand when the modernism started to establish. Like other buildings cultural space expressed modernized elevation and space formation with ferroconcrete building. However until Japan's colonial period the cultural areas were not used for public but for Japanese authority class's social gatherings only. Consequently, unlike other buildings the classical elements that could express these characters were more used in cultural spaces especially in theater and exhibition areas. This distinctiveness didn't appear separately but according to type of rooms in one space. Once more, place like assembly space was expressed with modernism, special or recreation rooms where people pursue a comfort were expressed with decorative style. Also a special theater which was used for only one person was generally expressed with western style to represent the stem character such as power.

• 한국환경농학회지
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• 제30권4호
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• pp.382-389
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• 2011

BACKGROUND: Light-emitting diodes (LEDs) with lower electric cost and the specific wavelength have been considering as a novel light source for plant production in greenhouse conditions as well as in a closed culture system. Supplementary lighting for day-length extension was considered as light intensity, light quality, and/or photoperiod control on plant growth and development. Effects of supplementary blue or red LED radiation with lower light intensity on growth of Ageratum (Ageratum houstonianum Mill., cv. Blue Field), African marigold (Tagetes erecta L., cv. Orange Boy), and Salvia (Salvia splendens F. Sello ex Ruem & Schult., cv. Red Vista) were discussed during sunrise and sunset twilight in the experiment. METHODS AND RESULTS: Supplementary lighting by blue and red LEDs for 30 (Treatment B30; R30) or 60 (Treatment B60; R60) min. per day were established in greenhouse conditions. Photosynthetic photon flux for supplementary radiation was kept at $15{\mu}mol\;m^{-2}\;s^{-1}$ on the culture bed. Natural condition without supplementary light was considered as a control. The highest shoot and root dry weights were shown in African marigold exposed by red light for 60 min. per day. Supplementary blue and red lighting regardless of the radiation time significantly stimulated development of lateral branches in African marigold. Stem growth in Ageratum and Salvia seedlings was significantly promoted by red radiation as well as natural light. CONCLUSIONS: Extending of the radiation time at sunrise and sunset twilight using LEDs stimulated reproductive growth of flowering plant species. Different characteristics on growth under supplementary blue or red lighting conditions were also observed in the seedlings during supplementary radiation.