Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Comparison of Bone Marrow Stromal Cells with Fibroblasts in Wound Healing Accelerating Growth Factor Secretion

골수기질세포 및 섬유아세포의 창상치유 촉진 성장인자 분비능 비교

  • Kim, Se-Hyun (Department of Plastic Surgery, College of Medicine, Korea University) ;
  • Han, Seung-Kyu (Department of Plastic Surgery, College of Medicine, Korea University) ;
  • Yoon, Tae-Hwan (Department of Plastic Surgery, College of Medicine, Korea University) ;
  • Kim, Woo-Kyung (Department of Plastic Surgery, College of Medicine, Korea University)
  • 김세현 (고려대학교 의과대학 성형외과학교실) ;
  • 한승규 (고려대학교 의과대학 성형외과학교실) ;
  • 윤태환 (고려대학교 의과대학 성형외과학교실) ;
  • 김우경 (고려대학교 의과대학 성형외과학교실)
  • Received : 2005.08.31
  • Published : 2006.01.10
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Abstract

Cryopreserved fibroblast implants represent a major advancement for healing of chronic wounds. Bone marrow stromal cells, which include the mesenchymal stem cells, have a low immunity-assisted rejection and are capable of expanding profoundly in a culture media. Therefore, they have several advantages over fibroblasts in clinical use. The ultimate goal of this study was to compare the wound healing accelerating growth factor secretion of the bone marrow stromal cells with that of the fibroblasts and this pilot study particularly focuses on the growth factor secretion to accelerate wound healing. Bone marrow stromal cells and fibroblasts were isolated from the same patients and grown in culture. At 1, 3, and 5 days post-incubating, secretion of basic fibroblast growth factor(bFGF), vascular endothelial growth factor (VEGF), and transforming growth factor beta(TGF-${\beta}$) were compared. In TGF-${\beta}$ secretion fibroblasts showed 12~21% superior results than bone marrow stromal cells. In contrast, bFGF levels in the bone marrow stromal cells were 47~89% greater than that in fibroblasts. The VEGF levels of the bone marrow stromal cells was 7~12 fold greater than that of the fibroblasts. Our results suggest that the bone marrow stromal cells have great potential for wound healing accelerating growth factor secretion.

Keywords

Acknowledgement

Supported by : 보건복지부

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