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http://dx.doi.org/10.5010/JPB.2004.31.1.037

Influence of Plant Growth Regulators on Shoot Multiplication of Thymus quinquecostatus Celak.  

Lee, Keum-Young (Laboratory of Forest Biotechnology, Departmnet of Forest Resources, Dongguk University)
Kang, Ho-Duck (Laboratory of Forest Biotechnology, Departmnet of Forest Resources, Dongguk University)
Publication Information
Journal of Plant Biotechnology / v.31, no.1, 2004 , pp. 37-41 More about this Journal
Abstract
In vitro culture system was established to induce multiple shoots of Thymus quinquecostatus Celak. by investigating the effects of cytokinins. Stem explants were cultures on MS medium supplemented with either five drfferent plant growth regulators or their combinations under light or dark condition. The most effective cytokininsource was the combination of BA 1.0mg/L and TDZ 0.1mg/L for producing shoots (6.05$\pm$1.51), zeatin 2.0mg/L and TEZ 0.1mg/L for elongating shoots (3.27$\pm$0.66cm) under the light condition. In addition the most effective cytokinin was 2-ip 2.0mg/L for producing shoots(5.20$\pm$1.81), zeatin 2.0mg/L for elongating shoots(5.64$\pm$1.24cm)under the dark condition. Overall, the average percent for in vitro shooting was greater than 89.58％.
Keywords
Light and dark culture; plant growth regulators; shoot multiplication; Thymus quinquecostatus;
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