• 생명과학회지
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• 제26권2호
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• pp.220-225
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• 2016

본 연구는 치과 병의원 진료실 주변환경과 치과종사자의 휴대전화에서 분리된 coagulase-negative staphylococci (CNS)의 분리율과 항균제 내성양상 및 분자 역학적 특성을 분석하고자 하였다. 2014년 12월부터 2015년 1월까지 부산지역 10개의 치과 병의원에서 총 154개의 샘플을 수집하여 MALDI-TOF분석법을 통해 동정하였다. 항균제 감수성검사는 디스크 확산법을 시행하였고 mupA, mecA 유전자 보유현황 및 SCCmec type은 PCR과 염기 서열분석에 의해 결정하였다. 154개의 샘플 중 10개(6.5%)에서 CNS 균주(Staphylococcus epidermidis 5주, Staphylococcus capitis 2주, Staphylococcus warneri 2주, Staphylococcus haemolyticus 1주) 가 분리되었다. 항균제 감수성검사에서 penicillin 10주, mupirocin 6주, gentamicin 5주, tetracycline 3주 및 cefoxitin과 erythromycin 2주가 내성이었고 clindamycin, ciprofloxacin, teicoplanin, trimethoprim-sulfamethoxazole에 내성인 세균은 없었다. 2개의 CNS균주(S. warneri, S. haemlyticus)에서 mecA 유전자가 검출되었고 1개의 CNS균주(S. epidermidis)에서 mupA가 확인되었다. Methicillin 내성 CNS균주 가운데 1주는 SCCmec I형이었고 1균주는 SCCmec의 유전형이 구분되지 않았다. 본 연구를 통하여 다약제 내성을 보이는 CNS균주가 더 이상 우리나라 치과병의원 환경에서 드물지 않음을 알 수 있었다.

• Journal of Yeungnam Medical Science
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• 제4권2호
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• pp.15-21
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• 1987

• 식품기술
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• 제23권2호
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• pp.224-237
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• 2010

• 한국동물위생학회지
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• 제14권2호
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• pp.143-147
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• 1991

Between June and August 1990, the tonsils of 86 healthy pigs were examined for the presence of staphylococci. All of the pigs examined harboured Staphylococci in the tonsils, the most predominant Staphylococcus species was Staphylococcus aureus(45.3%) followed by Staph hyicus subsp chromogenes (20.9%), Staph hyicus subsp hyicus (16.3%), Staph hominis(4.7%), Staph simulans(2.3%) and Staph xylosus(1.2%), Unidentifiable species were isolated from 3(3.5%) of the 86 tonsils examined. Thirty-nine strains of Staph aureus were subjected to the biotyping scheme of Hajek & Marsalek all the strains were classified as biotype B.

• BMB Reports
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• 제28권5호
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• pp.443-450
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• 1995

Cloned staphylococcal peptidoglycan hydrolase was used in determining the physiological characteristics of peptidoglycan hydrolase. This enzyme hydrolyzed the bacterial cell walls and released the N-terminal alanine, but not the reducing groups. This cloned gene product was localized in the cytoplasm of transformed Escherichia coli. Activity gels indicated the enzyme had an Mr of about 54,000, which was consistent with the deduced Mr from sequencing of the cloned gene. The activity bound to CM-cellulose but not DEAE-cellulose resin, indicating it as a basic protein. Enhanced enzyme activity in a low concentration of cations, and inhibited enzyme activity in a solution with dissolved phospholipids, suggested that the activity and the availability of this basic protein may be regulated between negatively charged and positively charged cellular molecules. The activity against boiled crude cell wall was much greater than against purifed cell wall, suggesting protein associated with crude cell wall may aid in the binding of the peptidoglycan hydrolase The cloned peptidoglycan hydrolase showed positive activity on whole cells of some lysostaphin-resistant coagulase-negative staphylococci. The cloned enzyme may be an alternative for lysostaphin for lysis of staphylococci.

• Journal of Nutrition and Health
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• 제31권1호
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• pp.80-87
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• 1998

Intestinal microflora in 17 breast-fed(BF) and 14 formula-fed (FF) newborn infants at about 1 week after birth were investigated to determine the floral differences of the two lactation groups. The fecal conditions were significantly different between the two groups in that the number of subjects who showed watery feces, and the times of defecation per day, were higher in the BF group. In addition, the mean fecal PH of the BF group was lower than that of the FF group. The dominant species in the BF groups which showed the highest count among the species of microflora was bifidobacteria , while the dominant species in the FF group was streptococci. The frequency of staphylococci and the count of bifidobacteria were significantly higher in the BF group. However, the frquencies of streptococci and Peptococcaceae were higher in the FF group. Through the differences were not significant, the frequency of bvifidobacteria and the count of staphylococci were higher in BF group, whereas the frequencies of bacteriodes, clostridia, enterobacter and the count of clostridia were higher in FF group.

• 한국임상수의학회지
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• 제20권2호
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• pp.166-171
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• 2003

Milk samples were collected from a total of 418 quarters of 214 Holstein cows. Of these, samples which were positive on California Mastitis Test (CMT) and above 200,000 cells/ml by somatic cell counts were subjected to bacteriological examination and antimicrobial susceptibility test. Major pathogens responsible for mastitis included coagulase-negative staphylococci (34.3%), coagulase-positive staphylococci (21.5%), gran-negative rod (coliforms and noncoliforms: 12.6%) and Streptococcus spp. (8.4%). These strains were tested with 13 antimicrobial agents by the Kirby and Bauer standardized disc diffusion method. The isolated pathogens were mostly susceptible to amoxicillin and cephalothin, but were resistant to erythromycin.

• International Journal of Oral Biology
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• 제38권1호
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• pp.1-4
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• 2013

Staphylococcus species are one of prevalent pathogens found in hospitals. Microbes that are a primary cause of nosocomial infection were isolated from a dental and medical environment it may assist the reader to explain what this is and how it differs from the 'dental health care providers and ward health care providers'. To investigate the distribution of staphylococcus species in this environment, we used vitek II to measure drug sensitivity, and further performed biochemical testing. The isolation rate of staphylococcus species from the dental and medical environment was 100% but from dental health care providers and ward health care providers were 44.4% and 33.3%, respectively. In the analyses, staphylococcus species showed resistance to diffusion of cefoxitin and oxacillin discs. These staphylococci may be sufficiently positive for the mecA gene. Our results suggest that staphylococci might be an important cause of nosocomial infection in the dental clinic.

• 한국미생물·생명공학회지
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• 제51권3호
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• pp.293-295
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• 2023

Linezolid, the first oxazolidinone introduced into human clinical use, has become a last resort antibiotic in treatment of serious infections caused by Gram-positive pathogens, including methicillin-resistant staphylococci and vancomycin-resistant enterococci. Although oxazolidinones are strictly prohibited for use in food-producing animals, occurrence of linezolid-resistant staphylococci has recently been reported in livestock farms in Korea. Here, we report the complete genome sequence of an optrA-positive linezolid-resistant Staphylococcus rostri strain PJFA-333 isolated from a pig farm in Korea.

• 한국가축위생학회:학술대회논문집
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• 한국가축위생학회 1996년도 제19차 학술대회 및 정기총회
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• pp.75.3-76
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• 1996