• Journal of Microbiology and Biotechnology
• /
• 제21권7호
• /
• pp.719-727
• /
• 2011

Botulism is a neuroparalytic disease caused by Clostridium botulinum, which produces seven (A-G) antigenically diverse neurotoxins (BoNTs). BoNTs are the most poisonous substances known to humans, with a median lethal dose ($LD_{50}$) of approximately 1 ng/kg of body weight. Owing to their extreme potency and lethality, they have the potential to be used as a bioterrorism agent. The mouse bioassay is the gold standard for the detection of botulinum neurotoxins; however, it requires at least 3-4 days for completion. Attempts have been made to develop an ELISA-based detection system, which is potentially an easier and more rapid method of botulinum neurotoxin detection. The present study was designed using a synthetic gene approach. The synthetic gene encoding the catalytic domain of BoNT serotype B from amino acids 1-450 was constructed with PCR overlapping primers (BoNT/B LC), cloned in a pQE30 UA vector, and expressed in an E. coli M15 host system. Recombinant protein production was optimized at 0.5 mM IPTG final concentration, 4 h post induction, resulting in a maximum yield of recombinant proteins. The immunogenic nature of the recombinant BoNT/B LC protein was evaluated by ELISA. Antibodies were raised in BALB/c mice using various adjuvants. A significant rise in antibody titer (p<0.05) was observed in the Alum group, followed by the Titermax Classic group, Freund's adjuvant, and the Titermax Gold group. These developed high-titer antibodies may prove useful for the detection of botulinum neurotoxins in food and clinical samples.

• 한국철도학회:학술대회논문집
• /
• 한국철도학회 2008년도 추계학술대회 논문집
• /
• pp.380-386
• /
• 2008

The development of a new railway vehicle is under progress through the Next Generation High-Speed Rail Development Project in Korea. Its aim is to develope fundamental technology of the vehicle that can run over 400km/h. The new distributed traction bogie system, 'HEMU'(High-speed Electric Multiple Unit), will be used and is different from that of previously developed high speed railway vehicles. Previous vehicles adopted push-pull type system, which means one traction-car drives rest all of the vehicle. Due to the difference, investigation on dynamic behavior and its safety evaluation are necessary, as a part of verification of the design specification. In the paper, current progresses of researches are presented. And the High-Speed Railway vehicle system is evaluated for a dynamic characteristic simulation. Proper dynamic models including air-suspension system, wheel-rail, bogie and car-body is developed according to the vehicle simulation scenario. The basic platform for the development of dynamic solver is prepared using nodal, modal coordinate system and wheel-rail contact module. Operating scenario is prepared using commercial dynamic analysis program and used for development of dynamic model, which contains many parts such as carbodies, bogies and suspension systems. Furthermore, international safety standard is applied for final verification of the system. Finally, the reliability of the dynamic model will be verified with test results in the further researches. This research will propose a better solution when test results shows a problem in the parts and elements. Finally, the vehicle that has excellent performance will be developed, promoting academic achievement and technical development.

• 농업과학연구
• /
• 제48권1호
• /
• pp.171-178
• /
• 2021

A comparative study was conducted to examine the effects of mixed and separate gender feeding on growth performance of Korean native chickens (KNC) against a white semi-broiler (WSB) and a commercial broiler (CB) over five weeks. 576 chicks were used with eight birds per cage in a randomized complete block design with 18 replicates per breed. For the KNC lines, three groups of male (M), female (F) and mixed-gender (FM) were used. Fresh water and feed were supplied on an ad-libitum basis. Birds were fed a standard starter (d 1 - 22) and grower diet (d 23 - 35). Body weight (BW), feed intake, and shank length (SL) were measured weekly. From the BW and feed consumed data, the average daily feed intake (ADFI), average daily gain (ADG), and feed conversion ratio (FCR) were calculated. The commercial broiler showed higher performance (p < 0.05) for all the indices measured for the entire period. The CB group consumed more feed and were more feed-efficient thus grew faster. This group was followed by the white semi-broiler and the KNC, in order. An intra-breed comparison for KNCs revealed that the males showed better growth performance with longer SL (p < 0.05) compared to the female and mixed-gender groups. The results showed that other than the breed type, mixed and separate gender feeding impacted on the growth performance of the two lines of Korean native chickens. Males for both lines of KNC generally performed better for the parameters measured, as determined by a greater BW and reduced FCR.

• 대한토목학회논문집
• /
• 제43권5호
• /
• pp.535-542
• /
• 2023

교량의 내진성능을 파악하기 위해서는 대상 교량에 대한 내진성능평가 및 수치해석을 통해 검토하는 것이 일반적이다. 내진성능평가 및 수치해석을 통한 검토는 특정 대상교량에 대한 분석 방법으로 전국 교량을 관리하는 각각의 관리주체에서 이를 수행하기에는 많은 문제가 발생할 수 있다. 따라서 본 연구에서는 다양한 형식 및 특성을 지니고 있는 공용 교량에 대한 내진성능을 추정할 수 있도록 연구를 수행하였다. 내진성능 추정은 지진위험도평가 방법을 적용하였으며, 교량의 형식 및 제원에 대한 지진취약도 곡선을 산정하고 국내 내진설계기준을 적용하여 공용 중인 교량의 내진성능을 추정하였다. 또한 이를 플랫폼에 탑재하여 사용자가 국내 교량에 대한 내진성능 추정을 간편하게 검토할 수 있도록 서비스 항목을 구축하였다.

• 한국환경보건학회지
• /
• 제48권1호
• /
• pp.44-51
• /
• 2022

Background: Air pollution is increasing together with industrialization and urbanization. In order to reduce air pollution, public transportation is recommended rather than private cars, and the number of passengers using public transportation is increasing accordingly. This study observes the concentration of indoor pollutants in city buses over time. Through this means, we intend to suggest a plan to manage the indoor air quality in city buses. Objectives: The concentration of indoor pollution in public transportation was investigated from April 2021 to January 2022. Based on this, we evaluated the exposure to indoor pollutants. Methods: Six city bus lines in an industrial city were selected for the research, and indoor pollution was measured through IoT (Internet of Things)-based sensor-type measuring devices. The concentration of pollutants was measured every minute, and statistical data were constructed based on the measurement results. Results: In all the city buses studied, the average concentration of pollutants were below the guidelines. However, some measurement results showed cases of exceeding the guidelines. As a result of the analysis by time zone, there were more cases in which pollutants exceeded the standard value during rush hour compared to at other times. A risk assessment for PM₁₀ was performed by evaluating the excess mortality risk from exposure and the risk from inhalation exposure. Conclusions: All measured indoor pollutants in the city buses did not exceed the guidelines. Also, the risk assessment results were found to be within the level of safety. However, if a city bus is used for a long time, there is a possibility that there may be an impact on the human body due to inhalation exposure, so additional management is required.

• Archives of Plastic Surgery
• /
• 제49권3호
• /
• pp.346-351
• /
• 2022

Background Postmastectomy breast reconstruction (PMR) increases patient satisfaction, quality of life, and psychosocial well-being. There is scarce data regarding the safety of PMR in chronic anticoagulated patients. Perioperative complications can reduce patient satisfaction; therefore, it is important to elucidate the safety of PMR in these patients. Methods A retrospective case-control study of patients who underwent PMR with implants and were on chronic anticoagulation was performed at our institution. Inclusion criteria were women ≥ 18 years old. Exclusion criteria included autologous reconstructions, lumpectomy, and oncoplastic procedures. Two controls for every one patient on anticoagulation were matched by age, body mass index, radiotherapy, smoking history, type of reconstruction, time of reconstruction, and laterality. Results From 2009 to 2020, 37 breasts (20 patients) underwent PMR with implant-based reconstruction and were on chronic anticoagulation. A total of 74 breasts (40 patients) who had similar demographic characteristics to the cases were defined as the control group. Mean age for the case group was 53.6 years (standard deviation [SD] = 16.1), mean body mass index was 28.6 kg/m² (SD = 5.1), and 2.7% of breasts had radiotherapy before reconstruction and 5.4% after reconstruction. Nine patients were on long-term warfarin, six on apixaban, three on rivaroxaban, one on low-molecular-weight heparin, and one on dabigatran. The indications for anticoagulation were prior thromboembolic events in 50%. Anticoagulated patients had a higher risk of capsular contracture (10.8% vs. 0%, p = 0.005). There were no differences regarding incidence of hematoma (2.7% vs. 1.4%, p = 0.63), thromboembolism (5% vs. 0%, p = 0.16), reconstructive-related complications, or length of hospitalization (1.6 days [SD = 24.2] vs. 1.4 days [SD = 24.2], p = 0.85). Conclusion Postmastectomy implant-based breast reconstruction can be safely performed in patients on chronic anticoagulation with appropriate perioperative management of anticoagulation. This information can be useful for preoperative counseling on these patients.

• 한국환경농학회지
• /
• 제34권2호
• /
• pp.105-110
• /
• 2015

BACKGROUND: Soy isoflavones, structurally similar to endogenous estrogens, may affect human body through both hormonally mediated and non-hormonally related mechanisms. Heat processing could change chemical compositions. The effects of different thermal processes, boiling and HTHP(high temperature and high pressure) on the composition of isoflavone compounds and total amount of domestic soybeans were investigated in this study. METHOD AND RESULTS: Three different kinds of soybean samples were collected from RDA-Genebank. The samples were extracted using methanol, distilled water, and formic acid based solvent. Also the same solvents were used for mobile phase in UPLC/ToF/MS. All of the isoflavone compounds were analyzed based on the aglycone type of external standard for quantification. The standard calibration curve presented linearity with the correlation coefficient R2 > 0.98, analysed from 1 to 50 ppm concentration. The total isoflavone contents does not change by treatment within the same breed. While "boiling" and "HTHP" processes tend to increase the contents of aglycone and ${beta}$-glucosides, "fresh" soybeans retained the high concentration of malonylglucosides. CONCLUSION: These results have to be considered while developing an effective functional food, from the health while point of view using soybeans.

• 한국의류학회지
• /
• 제13권2호
• /
• pp.155-165
• /
• 1989

The purpose of this study was to investigate on the sleeve form variation according to arm movements for elderly women. This study was experimented using the Plaster gypsum. The subjects who were elderly women were divided into three groups according to Rorher Index. Arm movements were five type ($0^{\circ},\;45^{\circ},\;90^{\circ},\;135^{\circ},\;180^{\circ}$) to each the vertical motion in front and in side. The statistical analyses used in this study were Mean, Standard Deviation, Spearman's correlation coefficients, paired T-test, Kruskal-wallis one-way ANOVA and Duncan's multiple range test. The results were as follows: 1) Increasing the movements, the width of the sleeve increased and the height of the sleeve cap decreased but girth of the sleeve cap was not consistant. 2) When varied the rates of the three aspects of the sleeve, the height of the sleeve cap showed the largest variation rate. 3) There was a negative correlation between the height and the width of the sleeve. On the other hand, there was a positive correlation between the sleeve width and the sleeve girth. 4) There were the vertical motion differences between in front and in side on the three aspects of sleeve. 5) The sleeve width and the sleeve girth were different according to somatotypes but the height of the sleeve cap was not different. 6) According to the variation rates of the body surfaces in length of standard lines, the form of sleeve was changed more in the front than in the back. Maximum variation rate showed in the areas of $D_1-D_3,\;E_1-E_3$ in the horizontal lines and $A-E_2$ in vertical lines. 7) According to the comparative drawing of the heights of sleeve cap and sleeve girth, the sleeve basic pattern $S_1(A.H/4)$ was more suitable for the direction $0^{\circ}\;and\;45^{\circ}$. And the basic pattern S_6(A.H/5)$ was more suitable for the direction $90^{\circ}$, the pattern $S_7(A.H/6)$ suitable for the direction $135^{\circ}$, and the pattern $S_8(A.H/8)$ was more suitable for the direction $180^{\circ}$.

• 의료법학
• /
• 제19권1호
• /
• pp.3-20
• /
• 2018

약침행위는 한방의료에서 행하여져 오던 침구요법과 약물요법을 결합한 요법으로 최근에 특히 교통사고 환자를 중심으로 한방의료에서 행하여져 오고 있다. 하지만 약침행위에 사용되는 약제가 무엇인지도 분명하지 않으며 약침행위가 어떠한 효능을 가지고 있으며 어떠한 부작용의 위험이 있는지에 대해서도 거의 알려진 바가 없다. 우리나라에서는 양방의료와 한방의료를 구분하여 규율하고 있으며 양자의 규율방식 또한 상이하다. 특히 새로운 의료기술이 개발된 경우 엄격한 임상시험절차를 거쳐 정당한 의료행위로 허가되는 양방의료의 경우와는 달리 한방의료는 그와 같은 절차가 존재하지 아니한다. 한편 <의료법>상 무면허 의료행위는 의료면허가 없는 자가 의료행위를 하는 경우 뿐만 아니라 의료면허가 있는 의료인이 자신의 면허영역 이외의 의료행위를 하는 경우도 포함하고 있다. 따라서 양방의료와 한방의료의 경계가 무엇인가에 대한 논의는 매우 중요한 논의 중 하나이다. 의료행위가 면허에 의하여 허가되며 보호받는 이유 중 가장 큰 것은 의료행위 자체가 가지고 있는 인간의 생명 신체에 대한 침해 가능성 때문이다. 새로운 의료행위가 출현한 경우, 그것이 양방의료의 영역이건 한방의료의 영역이건 정당화의 근거로 인간의 생명 신체에 대한 위험성을 항상 고려하여야 한다. 현재 이루어지는 약침행위는 아직 그러한 검증을 거치지 아니한 것으로 판단된다.

• 생명과학회지
• /
• 제16권6호
• /
• pp.960-963
• /
• 2006

본 연구는 비슷한 강도의 규칙적인 수영과 트레드밀 훈련이 흰쥐의 체중, 지방량, 혈중 triglyceride, insulin, 및 랩틴 호르몬 변화에 미치는 영향을 살펴보고 운동이 비만에 미치는 영향을 과학적으로 분석하고자 하였다. 본 연구의 결과로 규칙적인 훈련은 체중과 지방량을 감소시킬 뿐만이 아니라 혈중 triglyceride, insulin, 및 랩틴 호르몬을 감소시킴으로 비만 예방에 큰 효과가 있는 것으로 알려졌으며, 같은 기간의 비슷한 강도의 운동에서 수영 훈련 보다는 트레드밀 위에서 달리는 운동이 비만 예방에 더 큰 효과가 있는 것으로 나타났다. 추후 서로 다른 운동을 부여하여 운동생리학적인 변화를 비교할 뿐만이 아니라 이러한 원인을 규명하는 기전적인 연구가 진행되어져야 한다고 본다.