• 대한한의학방제학회지
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• 제18권1호
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• pp.95-103
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• 2010

Objectives : To develop and validate HPLC-PDA methods for simultaneous determination of seven constituents in Samsoeum(SSE). Methods : Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array(PDA) detection at 254 and 280 nm, were used for quantification of the seven marker components of SSE. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Results : Calibration curves were acquired with $r^2$>0.9997, and the relative standard deviation (RSD) values (%) for intra- and inter-day precision were less than 3.0%. The recovery rate of each compound was in the range of 100.07-112.65%, with an RSD less than 4.0%. The contents of seven compounds in SSE were 1.24-10.53 mg/g. Conclusions : The established method will be helpful to improve quality control of SSE.

• BMB Reports
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• 제51권12호
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• pp.609-610
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• 2018

Glycosylation is one form of protein modification and plays a key role in protein stability, function, signaling regulation and even cancer. NleB and SseK are bacterial effector proteins and possess glycosyltransferase activity, even though they have different substrate preferences. NleB/SseKs transfer the GlcNAc sugar to an arginine residue of host proteins, leading to reduced $NF-{\kappa}B-dependent$ responses. By combining X-ray crystallography, NMR, molecular dynamics, enzyme kinetic assays and in vivo experiments, we demonstrated that a conserved HEN (His-Glu-Asn) motif in the active site plays a key role in enzyme catalysis and virulence. The lid-domain regulates the opening and closing of the active site and the HLH domain determines the substrate specificity. Our findings provide evidence for the enzymatic mechanism by which arginine can be glycosylated by SseK/NleB enzymes.

• 한국수산과학회지
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• 제52권1호
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• pp.81-86
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• 2019

The common hallmark of several neurodegenerative disorders, including Alzheimer's disease (AD), is the presence of chronic neuroinflammation, which contributes to the loss of neuronal structure and function. This study investigated the effects of an ethanolic extract of Sargassum serratifolium (SSE) in a lipopolysaccharides (LPS)-induced murine neuroinflammation model. Mice were administered SSE (100 mg/kg body weight) or vehicle for 5 days by oral gavage, and then treated with LPS or saline by intraperitoneal injection. Thereafter, the brain tissues were collected, and the expression of pro-inflammatory cytokines was analyzed by quantitative real-time RT-PCR. There was a marked increase in the spleen weight index in the LPS-treated groups, which indicated the induction of acute systemic inflammation. Based on significant increases in the levels of IL-1 and IL-6 expression, the induction of neuroinflammation was also evident in the cortex and hippocampus of the LPS-treated groups. The overall expression of IL-1 and IL-6 was decreased slightly by SSE administration, compared with the LPS group, and a marked change in IL-1 was observed in the cortex of the SSE-treated (SSE/LPS) group. These results suggest that SSE has potential as an anti-neuroinflammatory nutraceutical.

• 대한정형도수물리치료학회지
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• 제25권1호
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• pp.9-19
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• 2019

Background: This study was planned to assess and compare the effectiveness of cervical region stabilization exercise and strengthening exercise on pain and range of motion in patient with chronic neck pain. Methods: Fifty-one patient with chronic neck pain were randomly allocated into strengthening and stabilization exercise (SSE) group (n=27) and Strengthening exercise (SE) group (n=24). The program was carried for 12 sessions, 3 days/week in 4weeks. Pain intensity with visual analog scale (VAS), cervical range of motion, deep cervical flexor strengthening were evaluated before and after the intervention. Results: After the program, pain intensity decreased in group SE and SSE were found (p<.05). Range of motion and deep cervical muscle strength increased in group SE and SSE were found(p<.05). The intergroup comparison showed that significant difference in range of motion and deep cervical muscle strength (p<.05). Conclusions: The study suggest that both SSE program and SE program might be a useful treatment for patients with chronic neck pain. However SSE program might be superior in improving cervical range of motion and deep cervical flexor strength compare to SE program.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2006년도 추계학술발표대회
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• pp.89-92
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• 2006

본 논문은 SIFT(Scale Invariant Feature Transform)알고리즘의 실시간처리 응용프로그램 작성기법을 기술하고 있는데, 단일 프로세서에서 병렬처리 기능을 지원하도록 설계된 SSE 명령어 집합을 사용하여 가우시안 convolution을 구현하고 있다. SIFT알고리즘의 Scale-space를 생성하는 과정에 수행되는 가우시안 Convolution은 연산시간이 과도하게 요구된다.[1] 2D의 가우시안 필터가 영상을 구성하는 모든 셀과 1:1로 연산을 수행하므로 이 연산의 소요시간은 영상의 가로, 세로 길이 그리고 필터의 크기에 비례하여 결정된다. 이 논문에서 제안하는 방법은 연산을 위해 CPU 내부로 한번 읽어 들인 픽셀자료에 대해 가능한 모든 연산을 SSE 명령어 집합을 사용하여 수행함으로써 병렬 연산에 의한 연산시간 절감과 메모리 접근 최소화를 통한 입출력시간 절감을 통해 전체 연산시간을 단축 하였다.

• 한국식품영양과학회지
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• 제37권4호
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• pp.490-496
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• 2008

꽈배기 모자반 추출물을 0.01%, 0.1%, 1% 농도로 첨가하여 이들이 모닝빵의 저장성과 품질에 미치는 영향에 대하여 알아보았다. 9일간 저장하며 일반 세균수와 곰팡이수를 측정한 결과 저장기간 전반에 걸쳐 첨가 농도가 증가할수록 미생물의 생육이 억제되었으며, 수분함량의 변화에 있어서는 꽈배기 모자반 추출물 처리구가 약 $5{\sim}6%$ 정도의 변화를 보인 반면 무처리구는 13% 정도의 큰 변화 폭을 보였다. 색도의 경우에는 추출물의 첨가농도가 높을수록 명도와 황색도는 감소하는 경향을 나타내었으며, 적색도는 이와 반대로 첨가 농도에 의존하여 증가하는 것으로 나타났다. 관능평가에서는 전체적인 호감도에서 0.01%와 0.1% 첨가구가 무처리구에 비하여 높은 점수를 받았으나 1% 첨가구에서는 가장 낮은 점수를 받았다. 이상의 결과를 종합해 볼 때 꽈배기 모자반 추출물을 0.1%정도 첨가할 경우 빵의 저장성, 품질 증진 및 관능 개선 효과가 있을 것으로 사료된다.

• 한국과학교육학회지
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• 제39권3호
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• pp.321-335
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• 2019

본 연구는 학생 경험이 과학 자기효능감 향상에 어떻게 영향을 주었는지를 탐색하여, 이를 바탕으로 과학 자기효능감 향상 과정에 대한 구조적 모형을 제안하였다. 본 연구에는 자연계열 고등학생 25명이 연구에 참여하였다. 근거이론에 의한 면담 자료의 코딩을 통해 과학 자기효능감과 관련된 10개의 범주를 도출하고 패러다임 분석을 통해 과학 자기효능감 향상 과정 모형을 개발하였다. 연구 결과, '과학 학습에 대한 기대감'을 가진 학생들이 자연계열을 선택하여 과학을 학습하는 과정에서 '과학 학습에 대한 개인 역량'과 '외부에서의 영향'의 맥락 작용을 통해 과학 자기효능감의 향상이 이루어지며, '내재적 불안 요인'과 '외재적 요인'의 중재적 조건을 받으며, 도입, 향상, 조절, 결과의 4단계를 순환하는 것을 알 수 있었다. 이때 중심현상은 '과학 자기효능감 향상'이다. 본 연구 결과는 교육현장에서 학생의 과학 자기효능감에 영향을 주는 교수전략 개발에 필요한 기초 정보를 제공한다.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.440-447
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• 2003

Up until today, the key to contouring has been resumed in these two alternatives, either limiting the adipocyte storing capacity by modulating lipogenesis, or by stimulating lipolysis to eliminate adipocyte lipid content. Another interesting way could be the regulation of adipocyte differentiation. In this work, we have evaluated the effect of a brown algal extract of Sphacelaria scoparia (SSE) on the differentiation of pre-adipocytes into adipocytes. A pre-adipocyte line (3T3-L 1) was used. The differentiation was evaluated by the measure of produced lipids thanks to red oil coloration and spectrophotometry, and also by the expression of adipocyte differentiation markers: enzymes such as fatty acid synthase (FAS) and stearoyl CoA desaturase (SCD), or membrane proteins such as glucose transporters (GLUT -4) and fatty acid transporters (FAT) expressed on the surface of human adipocytes. These genes are under control of two transcription factors: CAAT-enhancer binding protein (c/EBP alpha) and sterol response element binding protein (SREBP1). All these markers were analysed at different stages of differentiation by RT -PCR. Sphacelaria extract (SSE) inhibits pre-adipocytes differentiating into adipocytes following a dose-dependant relation, using a kinetics similar to retinoic acid. It decreases the expression of mRNA specific to FAS, FAT, GLUT -4, SCD1, c/EBP alpha and SREBP1. Moreover, SSE regulated on collagen 1 and collagen 4 expression. A stimulation of collagen 1 was also measured in human skin fibroblasts. Thus, SSE performs as a genuine differentiation inhibitor and not only as a lipogenesis inhibitor, and could be used in slimming products.

• 한국직업건강간호학회지
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• 제6권2호
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• pp.156-167
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• 1997

In Korea, based on the Revised Law of Occupational Safety and Health a new entity of institution was set up in 1990 to provide occupational health services to SSE in which three sorts of personnel as a team have to be involved. These institutions, in charge of scores to hundreds of workplace area-wide, have been providing occupational health services without payment from employers or employees, and government reimburses through the Occupational Injury Prevention Insurance since 1993. As a service provider, a team is composed of doctors, nurses and industrial hygienists. Undergraduate and postgraduate educations for the SSE occupational health are not specified and the question on the performance of the personnel has been raised. This study was designed to analyze the facilitating factors of and barriers to the performance and its improvement of these personnel. In 1997, the survey was conducted with all 58 institutions. Structured questionnaires were mailed to 200 personnel who were providing the occupational health service for SSE. The response rate was 51.7% for doctors, 58.6% for nurses, and 60.3% for industrial hygienist, respectively. Results are as follows : 1) There is a guideline for occupational service mandated by the government. Under the guideline, the minimum frequency of visiting workplace is assured with six times of doctors, 17 times for nurse and industrial hygienist in a year. There are one doctor for every 200 factories, one nurse and one hygienist for every 100 factories. 2) All respondents have basic qualification for occupational health service. About 16.7%. of doctors are certified in industrial medicine or preventive medicine, and 64.7% of industrial hygienists had first grade certification. Totally 66.7% of personnel have been involved in occupational health for more than one year. 3) As a support system for the performance improvement, 66.3% respond that they have been provided with educational materials, advice related to industrial environment and guidance of MSDS from Korea Industrial Safety Corporation. Most respondents indicate the lack of concern of employers and employees as a main barrier to the improvement of the service. Also they are in the need of the training opportunity more focused on SSE. The Governments policy for SSE is a principal facilitating factor. Training program focused on SSE situation, manpower, technical support, etc. are areas to be improved to have a better occupational health service for SSE in Korea.

• 한국전산구조공학회논문집
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• 제24권3호
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• pp.289-294
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• 2011

본 논문에서는 유한요소모델을 사용하여 원자력 발전용 해수여과장치에 대한 동적 내진해석을 수행하였다. 장치의 검증을 위해서 운전기준지진(Operating Basis Earthquake, OBE)과 안전정지지진(Safe Shutdown Earthquake, SSE)이 설계하중으로 작용하였을 때 부재에 미치는 영향을 평가하였다. 해석대상은 유한요소법을 사용하여 수학적 모델링을 완성하였고, 층응답스펙트럼(Floor Response Spectrum, FRS)에 따른 지진하중과 사하중 등을 적용하여 해석을 수행하였다. 해석된 해수여과장치의 최대변위는 OBE 조건에서 2.5mm이고, SSE 조건에서 최대변위는 4.6mm이다. 최대응력은 OBE 조건에서 24MPa, SSE 조건에서 44MPa이며, 이 값은 재료의 항복강도의 각각 18%, 27% 수준이다. 이에 따라 지진하중 조건에 따른 해수여과장치의 구조적 안전성이 제시되었다.