• 제목/요약/키워드: spot detection

검색결과 265건 처리시간 0.027초

Multiplex RT-PCR Assay for the Detection of Apple stem grooving virus and Apple chlorotic leaf spot virus in Infected Korean Apple Cultivars

  • Park, Hong-Lyeol;Yoon, Jae-Seung;Kim, Hyun-Ran;Baek, Kwang-Hee
    • The Plant Pathology Journal
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    • 제22권2호
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    • pp.168-173
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    • 2006
  • To develop the diagnostic method for the viral infection in apple, the partial genes corresponding to the N-terminal region of RNA polymerase of Apple stem grooving virus (ASGV) and coat protein of Apple chlorotic leaf spot virus (ACLSV) were characterized from the infected apple cultivars in Korea. Based on the nucleotide sequences of the characterized partial genes, the virus gene-specific primers were designed for the detection of ASGV and ACLSV infected in species of Malus. The RT-PCR using the primers for the genes of ASGV and ACLSV successfully gave rise to 404 and 566 bp DNA fragments, respectively. Using those viral gene-specific primers, the multiplex RT-PCR assays were also established to diagnose the mixed infection by ASGV and ACLSV simultaneously. Furthermore, the control primers, which have to be included for the RT-PCR as an internal control, were designed using the nucleotide sequence of the gene encoding elongation factor $1{\alpha}(EF1{\alpha})$. This multiplex RT-PCR including the control primers provides more reliable, rapid and sensitive assay for the detection of ASGV and ACLSV infected in Korean apple cultivars.

카메라 기반의 측후방 차량 검출 및 추적 방법 (A Method for Rear-side Vehicle Detection and Tracking with Vision System)

  • 백승환;김흥섭;부광석
    • 한국정밀공학회지
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    • 제31권3호
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    • pp.233-241
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    • 2014
  • This paper contributes to development of a new method for detecting rear-side vehicles and estimating the positions for blind spot region or providing the lane change information by using vision systems. Because the real image acquired during car driving has a lot of information including the target vehicle and background image as well as the noises such as lighting and shading, it is hard to extract only the target vehicle against the background image with satisfied robustness. In this paper, the target vehicle has been detected by repetitive image processing such as sobel and morphological operations and a Kalman filter has been also designed to cancel the background image and prevent the misreading of the target image. The proposed method can get faster image processing and more robustness rather than the previous researches. Various experiments were performed on the highway driving situations to evaluate the performance of the proposed algorithm.

저항용접에서의 용접성 평가에 관한 연구 (A Study on the Evaluation of Weldability in Spot Welding)

  • 홍민성;김종민
    • 한국공작기계학회:학술대회논문집
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    • 한국공작기계학회 2003년도 추계학술대회
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    • pp.301-306
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    • 2003
  • Spot welding is frequently used for industrial purpose, such as automobile and aerospace industries and household appliances due to its high performance. In these day, robotization and systemization of welding process made it possible to produce more precise or smaller electric parts. And when it comes to welding of steel sheet, the size of nugget must be getting smaller. Therefore, welding conditions are limited to avoid defects, such as deformation, damage, weakening of joining area. In this research, the measurement of the nugget size by the nondestructive inspection has been conducted. As a result, the right estimation of the nugget size and void defects, the detection of corona bond near joining area, the selection of the optimum ultrasonic mode, and set up for ultrasonic inspection are studied. From the trustworthy solutions of nugget size estimated by results of measurement, the optimum inspection conditions depending upon the width of welding parts are determined as well.

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볼록 거울 및 단일 카메라를 이용한 실내에서의 전 방향 위치 검출 방법 (The Indoor Position Detection Method using a Single Camera and a Parabolic Mirror)

  • 김지홍;김희선;이창구
    • 제어로봇시스템학회논문지
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    • 제14권2호
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    • pp.161-167
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    • 2008
  • This article describes the methods of a decision of the location which user points to move by an optical device like a laser pointer and a moving to that location. Using a conic mirror and CCD camera sensor, a robot observes a spot of user wanted point among an initiative, computes the location and azimuth and moves to that position. This system offers the brief data to a processor with simple devices. In these reason, we can reduce the time of a calculation to process of images and find the target by user point for carrying a robot. User points a laser spot on a point to be moved so that this sensor system in the robot, detecting the laser spot point with a conic mirror, laid on the robot, showing a camera. The camera is attached on the robot upper body and fixed parallel to the ground and the conic mirror.

Detection of Xanthomonas axonopodis pv. citri on Satsuma Mandarin Orange Fruits Using Phage Technique in Korea

  • Myung, Inn-Shik;Hyun, Jae-Wook;Cho, Weon-Dae
    • The Plant Pathology Journal
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    • 제22권4호
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    • pp.314-317
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    • 2006
  • A phage technique for detection of Xanthomonas axonopodis pv. citri, a causal bacterium of canker on Sastuma mandarin fruits was developed. Phage and ELISA techniques were compared for their sensitivity for detection of Xanthomonas axonopodis pv. citri on orange fruits. Both of techniques revealed a similar efficiency for the bacterial detection; the pathogenic bacteria were observed in pellet from the fruits with over one canker spot with below 2 mm in diameter. In field assays, the increase of phage population(120%) on surface of the fruits related to the disease development one month later indicated that the bacterial pathogens inhabit on the surface. The procedure will be effectively used for detection of only living bacterial pathogen on fruit surfaces of Satsuma mandarin and for the disease forecasting.

THE EFFICIENT METHOD TO DETECT DEFECTIVE DETECTOR OF THE SWIR BAND OF SPOT 4

  • Jung Hyung-sup;Kang Myung-Ho;Lee Yong-Woong;Won Joong-Sun
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 2005년도 Proceedings of ISRS 2005
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    • pp.130-133
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    • 2005
  • This paper presents the efficient method to detect the defective detectors of the SWIR band of SPOT 4. The key of this method are to flatten the baseline of the data using high pass band filter instead of differentiation. This method is made up six steps. First step is to apply image enhancement techniques to enhance the lines imaged by defective detector and improve the quality of an image. Second step is processed by summing the enhanced image in line direction. These summed data have the peaks that represent the defective detectors and the curved baseline characterized by the reflectivity of Earth surface. In order to exactly detect these peaks, third step is to flatten the curved baseline using high pass filtering in the frequency domain. In fourth step, the data with flat baseline is normalized to have zero mean and unity standard deviation. In fifth step, the defective detectors are detected using $99.9\%$ confidence interval. Finally, after removing the detected ones in summed data, the steps from third to five are iterated. Three SPOT 4 images, which have different reflectivity of Earth surface and different sensor, were used to validate this method. The overall accuracy of detection for three images was $97.9\%$. This result shows that this method can detect efficiently the lines made by defective detectors.

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스마트 기기를 이용한 화점 표출 시스템의 설계 및 구현 (The Design and Implementation of the Fire Spot Display System Using s Smart Device)

  • 김상기;김동현
    • 한국전자통신학회논문지
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    • 제13권6호
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    • pp.1287-1292
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    • 2018
  • 화재가 발생하면 효율적인 화재 진압을 위하여 소방관은 화점 정보를 포함한 화재 관련 정보가 필요하다. 그러나 기존의 화재 감지 시스템은 현장의 소방관에게 화재 정보를 시각적으로 전달하지 못하는 문제가 있다. 이 논문에서는 이를 해결하기 위하여 클라우드 서버를 이용한 화점 표출 시스템을 제안한다. 제안한 시스템에서 각 건물에 설치된 센서들은 온도 및 가스 정보를 수집하고 무선 네트워크를 통하여 클라우드 데이터베이스에 저장한다. 현장의 소방관을 위하여 클라우드 데이터베이스에 저장된 특정 건물의 도면과 함께 화점 데이터의 상세와 시간에 따른 이력을 스마트 기기에 표출한다.

White spot syndrome virus (WSSV)의 VP28에 대한 단클론 항체 생산 (Production of monoclonal antibodies against VP28 of white spot syndrome virus (WSSV))

  • 방지형;김위식;김춘섭;김종오;오명주
    • 한국어병학회지
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    • 제32권1호
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    • pp.45-48
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    • 2019
  • We developed and subsequently characterized mouse monoclonal antibodies (MAbs) against recombinant VP28 structural protein (rVP28) of white spot syndrome virus (WSSV). We established six hybridoma clones secreting MAbs against rVP28: 15A11, 20G6, 31H2, 34H6, 38D1 and 43A1. All six MAbs recognized the 25 kDa of protein in gill homogenates of WSSV-infected shrimp by western blot analysis, while no reactivity was observed in gill homogenates of normal shrimp. Moreover, high enzyme-linked immunosorbent assay (ELISA) optical density (OD) values (0.8-2.68) were observed in the hemolymphs from WSSV-infected shrimp, while low OD values (less than 0.24) were recorded in the hemolymphs from normal shrimp, by using these six MAbs produced in this study. These results suggest that these six MAbs are useful for the detection of WSSV.

DS-CDMA 셀룰러 시스템에서의 음성검출을 사용한 동적코드할당방식 (Dynamic code allocation using voice activeity detection in DS-CDMA cellular system)

  • 유명수;양영님;고종하;이정규
    • 한국통신학회논문지
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    • 제22권6호
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    • pp.1302-1310
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    • 1997
  • In this paper, we propose a dynamic code allocation strategy using voice activity detection and evaluate the performance of a dynamic code allocation strategy using voice activeity detection in DS-CDMA system. Proposed method allocates code to mobile terminal according to the residual capacity computed by SIR in the base station. In hot spot traffic loading cell, we find that the performance of proposed method is better than that of a fixed code assignment strategy using voice activity detection. Also, we find that the proposed method provide much improvement in blocking probability against the dynamic code assignment strategy withoug voice activity detection.

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아르곤 레이저 광감각법의 법랑질 우식증 조기탐지 효과에 관한 연구 (A STUDY ON THE EARLY DETECTION OF ENAMEL CARIES BY THE LUMINESCENCE EXCITED BY ARGON LASER)

  • 이난영;이창섭;이상호
    • 대한소아치과학회지
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    • 제24권1호
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    • pp.313-324
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    • 1997
  • The aim of the present study was to describe an safe and convenient method for the early detection of enamel caries using laser fluorescence. Fluorescence from natually carious lesion of human teeth illuminated by an argon laser(488nm) was observed and photographed using barrier filter. Intact enamel was found to fluorescence with a yellowish light. Whereas, incipient caries lesions in the enamel were dearly visible as dark areas in contrast to the fluorescence surroundings. For evaluation of accuracy of this method, lesion depth measured by the laser fluorescence in light microscope was compared with that polarizing microscope. The results from the present study can be summarized as follows : 1. Enamel caries of smooth surface was observed as pale white spot and undefined outline in ordinary light. Whereas, lesion was clearly visible as dark spot in laser fluorescence. 2. There was no difference between ordinary light view and laser fluorescence in occlusal surface and interproximal surface. 3. There was no significant difference between the lesion depth observed by laser fluorescence with light microscope and polarizing microscope. Apparent correlation exists between two groups.

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