The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Detection of Xanthomonas axonopodis pv. citri on Satsuma Mandarin Orange Fruits Using Phage Technique in Korea

  • Myung, Inn-Shik (Plant Pathology Division, National Institute of Agricultural Science and Technology) ;
  • Hyun, Jae-Wook (Subtropical Environmental Division, National Institute of Subtropical Agriculture) ;
  • Cho, Weon-Dae (Plant Pathology Division, National Institute of Agricultural Science and Technology)
  • Published : 2006.12.01
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Abstract

A phage technique for detection of Xanthomonas axonopodis pv. citri, a causal bacterium of canker on Sastuma mandarin fruits was developed. Phage and ELISA techniques were compared for their sensitivity for detection of Xanthomonas axonopodis pv. citri on orange fruits. Both of techniques revealed a similar efficiency for the bacterial detection; the pathogenic bacteria were observed in pellet from the fruits with over one canker spot with below 2 mm in diameter. In field assays, the increase of phage population(120%) on surface of the fruits related to the disease development one month later indicated that the bacterial pathogens inhabit on the surface. The procedure will be effectively used for detection of only living bacterial pathogen on fruit surfaces of Satsuma mandarin and for the disease forecasting.

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References

  1. Anderson, E. S. and Williams, R. E. O. 1956. Bacteriophage typing of enteric pathogens and staphylococci and its use in epidemiology. J. Clin. Pathol. 9:994-127
  2. Alverez, A. M., Benedict, A. A., Mizumoto, C. Y., Pollanrd, L. W. and Civerollo, E. L. 1992. Analysis of Xanthomonas campes-tris pv. citri and X. a. pv. citrumello with monoclonal antibodies. Phytopathology 81:857-867 https://doi.org/10.1094/Phyto-81-857
  3. Anonymous. 1990. Quarantine procedure: Xanthononas campestris pv. citri. Bull. Eur. Mediterr. Plant Prot. Org. 20:263-272
  4. Billing, E. 1963. The value of phage sensitivity tests for the identificaton of phytopathogenic Pseudomonas spp. J. Appl. Bact. 33:478-491
  5. Billing, E. 1970. Further studies on the phage sensitivity tests for the identification of phytopathogenic Pseudomonas spp. J Appl. Bact. 33:478-491 https://doi.org/10.1111/j.1365-2672.1970.tb02224.x
  6. Civerolo, E. L. 1984. Bacterial canker disease of citrus. J. Rio Grande Val. Hortic. Soc. 37:127-146
  7. Gross, D. C., Powelson, M. L., Regner, K. M. and Radamaker, G. K. 1991. A bacteriophage-typing system for surveying the diversity and distribution of strains of Erwinia carotovora subsp. carotovora in potato fields. Phytopathology 81:220-226 https://doi.org/10.1094/Phyto-81-220
  8. Hartung, J. S., Daniel, J. F. and Pruvost, O. P. 1993. Detection of Xanthomonas campestris pv. citri by the polymerase chain reaction method. Appl. Environ. Microbiol. 59: 1143-1148
  9. Koizumi, M. 1985. Citrus canker: the world situation. In: Citrus Canker. An International Perspective, ed. by L. W. Timmer, pp. 2-7. Proc. Symp. Intst. Food Agric. Sci., University of Florida
  10. Myung, I.-S., Nam, K. W. and Cho, Y. 2002. Cultural characteristics of Xanthomonas axonopodis pv. citri bacteriophages CP1 from Korea. Plant Pathol. J. 18:333-337 https://doi.org/10.5423/PPJ.2002.18.6.333
  11. Myung, I.-S., Cho, Y., Lee, Y.-H. and Kwon, H.-M. 2001. Phage typing and lysotype distribution of Xanthomonas axonopodis pv. citri, the causal agent of citrus bacterial canker in Korea. Plant Pathol. J. 17:336-341
  12. Myung, I.-S., Nam, K.-W. and Kwon, H.-M. 2003. Dispersal of citrus bacterial canker caused by Xanthomonas axonopodis pv. citri in nursery plots of Unshiu orange. Plant Pathol. J. 19:205-209 https://doi.org/10.5423/PPJ.2003.19.4.205
  13. Schoulties, C. L., Civerolo, E. L., Miller, J. W., Stall, R. E., Krass, C. J., Poe, S. R. and DuCharme, E. P. 1987. Citrus canker in Florida. Plant Dis. 71:388-395 https://doi.org/10.1094/PD-71-0388