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http://dx.doi.org/10.5423/PPJ.2006.22.4.314

Detection of Xanthomonas axonopodis pv. citri on Satsuma Mandarin Orange Fruits Using Phage Technique in Korea  

Myung, Inn-Shik (Plant Pathology Division, National Institute of Agricultural Science and Technology)
Hyun, Jae-Wook (Subtropical Environmental Division, National Institute of Subtropical Agriculture)
Cho, Weon-Dae (Plant Pathology Division, National Institute of Agricultural Science and Technology)
Publication Information
The Plant Pathology Journal / v.22, no.4, 2006 , pp. 314-317 More about this Journal
Abstract
A phage technique for detection of Xanthomonas axonopodis pv. citri, a causal bacterium of canker on Sastuma mandarin fruits was developed. Phage and ELISA techniques were compared for their sensitivity for detection of Xanthomonas axonopodis pv. citri on orange fruits. Both of techniques revealed a similar efficiency for the bacterial detection; the pathogenic bacteria were observed in pellet from the fruits with over one canker spot with below 2 mm in diameter. In field assays, the increase of phage population(120%) on surface of the fruits related to the disease development one month later indicated that the bacterial pathogens inhabit on the surface. The procedure will be effectively used for detection of only living bacterial pathogen on fruit surfaces of Satsuma mandarin and for the disease forecasting.
Keywords
phage detection; Satsuma mandarin; Xanthomonas axonopodis pv. citri;
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