• Journal of Microbiology and Biotechnology
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• v.22 no.3
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• pp.431-435
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• 2012

In this study, we describe a novel approach to achieve replicative selectivity of conditionally replicative adenovirus that is based upon trans-splicing ribozyme-mediated replacement of cancer-specific RNAs. We developed a specific ribozyme that can reprogram human telomerase reverse transcriptase (hTERT) RNA to induce adenoviral E1A gene expression selectively in cancer cells that express the RNA. Western blot analysis showed that the ribozyme highly selectively triggered E1A expression in hTERT-expressing cancer cells. RT-PCR and sequencing analysis indicated that the ribozyme-mediated E1A induction was caused via a high fidelity trans-splicing reaction with the targeted residue in the hTERT-expressing cells. Moreover, reporter activity under the control of an E1A-dependent E3 promoter was highly transactivated in hTERT-expressing cancer cells. Therefore, adenovirus containing the hTERT RNA-targeting trans-splicing ribozyme would be a promising anticancer agent through selective replication in cancer cells and thus specific destruction of the infected cells.

• Genomics & Informatics
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• v.3 no.4
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• pp.172-174
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• 2005

Recent anti-cancer approaches have been based to target tumor-specifically associated and/or causative molecules such as RNAs or proteins. As this specifically targeted anti-cancer modulator, we have previously described a novel human cancer gene therapeutic agent that is Tetrahymena group I intron-based trans-splicing ribozyme which can reprogram and replace human telomerase reverse transcriptase (hTERT) RNA to selectively induce tumor-specific cytotoxicity in cancer cells expressing the target RNA. Moreover, the specific ribozyme has been shown to efficiently retard tumor tissues in xenograft mice which had been inoculated with hTERT-expressing human cancer cells. In this study, we assessed specificity of trans-splicing reaction in cells to evaluate the therapeutic feasibility of the specific ribozyme. In order to analyze the trans-spliced products by the specific ribozyme in hTERT-positive cells, RT, 5'-end RACE-PCR, and sequencing reactions of the spliced RNAs were employed. Then, whole analyzed products resulted from reactions only with the hTERT RNA. This study suggested that the developed ribozyme perform highly specific RNA replacement of the target RNA in cells, hence trans-splicing ribozyme will be one of specific agents for genetic approach to revert cancer.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2001.09a
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• pp.179-186
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• 2001

It has been known that lap splicing in the longitudinal reinforcement of bridge columns is not desirable for seismic performance, but it is sometimes unavoidable. Lap splices were usually be located in the plastic hinge region of most bridge columns that were constructed before the adoption of the seismic design provision of Korea Bridge Design Specification on 1992. This research is to evacuate the seismic performance of reinforced concrete bridge piers with lap splicing of longitudinal reinforcement in the plastic hinge region, and to develop the enhancement scheme of their seismic capacity by retrofitting with glassfiber sheets and to develop appropriate limited ductility design concept in low or moderate seismicity region. Nine test specimens in the aspect ratio of 4.0 were made with three confinement ratios and three types of lap splicing. Quasi-static tests under three different axial load levees were conducted. It has been observed that displacement ductility ratios of test columns with lap splicing were significantly reduced.

• Genomics & Informatics
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• v.17 no.3
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• pp.23.1-23.6
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• 2019

The acquisition of somatic mutations is the most common event in cancer. Neoantigens expressed from genes with mutations acquired during carcinogenesis can be tumor-specific. Since the immune system recognizes tumor-specific peptides, they are potential targets for personalized neoantigen-based immunotherapy. However, the discovery of druggable neoantigens remains challenging, suggesting that a deeper understanding of the mechanism of neoantigen generation and better strategies to identify them will be required to realize the promise of neoantigen-based immunotherapy. Alternative splicing and RNA editing events are emerging mechanisms leading to neoantigen production. In this review, we outline recent work involving the large-scale screening of neoantigens produced by alternative splicing and RNA editing. We also describe strategies to predict and validate neoantigens from RNA sequencing data.

• Journal of the Korean Society of Safety
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• v.25 no.6
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• pp.70-74
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• 2010

For each strand of wirerope sling, the international standards, ISO-8794, EN-13414 specify that the splice shall have five series of load carrying tucks. At least three of the load carrying tucks shall be made with the whole strand. And, the breaking force of the splice shall not be less than 70% or 80% of that of rope. But, There are no prescriptions for splicing types against different efficiency of each splicing type being used many workplace. In this study, analysis the work limit load efficiency according to variation of number of tucks and splicing types by experimental method As a result, the number of tucks 3+2 had the highest breaking efficiency.

• Molecules and Cells
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• v.40 no.1
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• pp.1-9
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• 2017

Serine and arginine-rich (SR) proteins are RNA-binding proteins (RBPs) known as constitutive and alternative splicing regulators. As splicing is linked to transcriptional and post-transcriptional steps, SR proteins are implicated in the regulation of multiple aspects of the gene expression program. Recent global analyses of SR-RNA interaction maps have advanced our understanding of SR-regulated gene expression. Diverse SR proteins play partially overlapping but distinct roles in transcription-coupled splicing and mRNA processing in the nucleus. In addition, shuttling SR proteins act as adaptors for mRNA export and as regulators for translation in the cytoplasm. This mini-review will summarize the roles of SR proteins as RNA binders, regulators, and connectors from transcription in the nucleus to translation in the cytoplasm.

• Genomics & Informatics
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• v.3 no.3
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• pp.80-85
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• 2005

HExDB is a database for analyzing exon and splicing pattern information in Homo sapiens. HExDB is useful for specific purposes: 1) to design primers for exon amplification from cDNA and 2) to understand the change of ORFs by alternative splicing. HExDB was constructed by integrating data from AltExtron which is the computationally predicted exon database, Ensemble cDNA annotation, and Affymetrix genome tile published recently. Although it may contain false positive data, HExDB is good starting point due to its sensitivity. At present, there areas many as 2,046,519 exons stored in the HExDB. We found that $16.8\%$ of the exons in the database was constitutive exons and $83.1\%$ were novel gene exons.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.46 no.7
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• pp.15-21
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• 2009

We proposed a fusion splicing method for low splicing loss between a single-mode fiber(SMF) and two different photonic crystal fibers(PCFs) such as a photonic bandgap fiber(PBGF) and highly nonlinear photonic crystal fiber(NL-PCF). The splicing loss between the SMF and PBGF is affected by air-hole collapse. Therefore, we optimized fusion splicer and reduced a splicing loss below 1.22 dB. We also inserted a Intra High Numerical Aperture(UHNA) fiber between the SMF and NL-PCF to achieve a splicing loss of below 2.59 dB.

• Composites Research
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• v.16 no.2
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• pp.26-32
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• 2003

Optical fibers, for splice, are stripped of their plastic coatings with a plastic stripper and cut off at the end. Therefore, stripped fibers often receive accidental damages and sustain small flaws or cracks. As a result, the breaking strength of a fiber splice made under normal conditions is reduced to about 0.4∼1 ㎏ on the average, nearly one-tenth of the fiber's strength. This makes it necessary to reinforce the splice. One of the most practical and reliable methods for optical fiber splicing is fusion splicing, comprising the steps of tripping the plastic coatings from the two fiber ends to be splice, placing the two bare fiber ends in an end-to-end position, and of fusion splicing, such as are fusion. Generally, steel bar (SB) sleeve is used to reinforce this fusion-splicing region. However, this type of sleeve has a critical defect to keep optical lose after bent by a sudden load. New type of composite spring (CS) sleeve is developed to make up for the weak points in the SB sleeve. This sleeve has an effect on restoration to the original state after eliminating the bending load. The optical spectrum analyzes results show the availability of reinforcement for the fusion splicing optical fiber using small diameter composite springs under the various loading conditions.

• Molecules and Cells
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• v.28 no.2
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• pp.111-117
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• 2009

The CHRM3 gene is a member of the muscarinic acetylcholine receptor family that plays important roles in the regulation of fundamental physiological functions. The evolutionary mechanism of exon-acquisition and alternative splicing of the CHRM3 gene in relation to transposable elements (TEs) were analyzed using experimental approaches and in silico analysis. Five different transcript variants (T1, T2, T3, T3-1, and T4) derived from three distinct promoter regions (T1: L1HS, T2, T4: original, T3, T3-1: THE1C) were identified. A placenta (T1) and testis (T3 and T3-1)-dominated expression pattern appeared to be controlled by different TEs (L1HS and THE1C) that were integrated into the common ancestor genome during primate evolution. Remarkably, the T1 transcript was formed by the integration event of the human specific L1HS element. Among the 12 different brain regions, the brain stem, olfactory region, and cerebellum showed decreased expression patterns. Evolutionary analysis of splicing sites and alternative splicing suggested that the exon-acquisition event was determined by a selection and conservation mechanism. Furthermore, continuous integration events of transposable elements could produce lineage specific alternative transcripts by providing novel promoters and splicing sites. Taken together, exon-acquisition and alternative splicing events of CHRM3 genes were shown to have occurred through the continuous integration of transposable elements following conservation.