• Journal of Pharmaceutical Investigation
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• 제34권5호
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• pp.393-399
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• 2004

For the efficient determination of activity against solid tumors, an in vitro tumor model that resembles the condition of in vivo solid tumors, is required. The purpose of this study was to establish a rapid culture method and viability assay for an in vitro 3-dimensional tumor model, multicellular spheroid (MCS). Among 12 human cancer cell lines, a few cell lines including DLD-1 (human colorectal carcinoma cells) formed fully compact MCS which was adequate for in vitro viability assay. DLD-1 MCS showed steady growth reaching $700\;{\mu}m$ diameter after 11 day culture. DLD-1 cells grown as MCS showed significant increase in $G_0/G_1$ phase compared to the monolayer cells (73.9% vs 45.7%), but necrotic regions or apoptotic cells were not observed. The cells cultured as MCS showed resistance to 5-FU (10.3 fold higher $IC_{50}$) compared to monolayers, however, tirapazamine (a hypotoxin) showed similar activity in both culture systems. In summary, MCS may be a valid in vitro model for activity screening of anticancer agents against human solid tumors and also exploitable for studying molecular markers of drug resistance in human solid tumors.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2008년도 춘계학술대회논문집
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• pp.934-938
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• 2008

To customize individual characteristics of HRTF, a spherical model has been used for structural modeling technique. A pseudo-code of prolate spheroidal HRTF caused by incident acoustic point source is already developed, and it can be used a head shadow filter for structural modeling of HRTF. In this research, to see the necessity and efficiency of spheroidal head modeling, ITD optimization is performed on CIPIC HRTF database. From given cost function, ITD-optimized spheroidal head model, whose ITD information is the most matched version of measured ITD information, is found by varying head parameters subject by subject. By comparing results of ITD-optimized spheroids and ITD-optimized spheres, we concluded that a spherical head model is more efficient way of generating head shadow effect than a spheroidal head model does.

• 천문학논총
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• 제30권2호
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• pp.297-299
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• 2015

In this paper we have examined the linear stability of triangular equilibrium points in the photogravitational restricted three body problem when both primaries are triaxial rigid bodies, the bigger one an oblate spheroid and source of radiation. The orbits about the Lagrangian equilibrium points are important for scientific investigation. A number of space missions have been completed and some are being proposed by various space agencies. We analyze the periodic motion in the neighbourhood of the Lagrangian equilibrium points as a function of the value of the mass parameter. Periodic orbits of an infinitesimal mass in the vicinity of the equilibrium points are studied analytically and numerically. The linear stability of triangular equilibrium points in the photogravitational restricted three body problem with Poynting-Robertson drag when both primaries are oblate spheroids has been examined by A. Kumar (2007). We have found the equations of motion and triangular equilibrium points for our problem. With the help of the characteristic equation we have discussed stability conditions. Finally, triangular equilibrium points are stable in the linear sense. It is further seen that the triangular points have long or short periodic elliptical orbits in the same range of ${\mu}$.

• Biomolecules & Therapeutics
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• 제29권2호
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• pp.205-210
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• 2021

Over 30 million prescriptions of NSAIDs (non-steroidal anti-inflammatory drugs) are issued every year. Considering that these drugs are available without a prescription as over the counter (OTC) drugs, their use will be astronomical. With the increasing use of NSAIDs, their adverse effects are drawing attention. Especially, stomach bleeding, kidney toxicity, liver toxicity, and neurological toxicity are reported as common. Ibuprofen, one of the extensively used NSAIDs along with aspirin, can also induce liver toxicity, but few studies are addressing this point. Here we examined the liver toxicity of ibuprofen and investigated whether co-exposure to ethanol can manifest synergistic effects. We employed 2D and 3D cultured human hepatoma cells, HepG2 to examine the synergistic hepatotoxicity of ibuprofen and alcohol concerning cell viability, morphology, and histology of 3D spheroids. As a result, ibuprofen and alcohol provoked synergistic hepatotoxicity against hepatocytes, and their toxicity increased prominently in 3D culture upon extended exposure. Oxidative stress appeared to be the mechanisms underlying the synergistic toxicity of ibuprofen and alcohol as evidenced by increased production of ROS and expression of the endogenous antioxidant system. Collectively, this study has demonstrated that ibuprofen and EtOH can induce synergistic hepatotoxicity, providing a line of evidence for caution against the use of ibuprofen in combination with alcohol.

• Journal of Microbiology and Biotechnology
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• 제32권9호
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• pp.1209-1216
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• 2022

To better understand the effects of PEGylation and biotinylation on the delivery efficiency of proteins, the cationic protein lysozyme (LZ) and anionic protein bovine serum albumin (BSA) were chemically conjugated with poly(ethylene glycol) (PEG) and biotin-PEG to primary amine groups of proteins using N-hydroxysuccinimide reactions. Four types of protein conjugates were successfully prepared: PEGylated LZ (PEG-LZ), PEGylated BSA (PEG-BSA), biotin-PEG-conjugated LZ (Bio-PEG-LZ), and biotin-PEG-conjugated BSA (Bio-PEG-BSA). PEG-LZ and Bio-PEG-LZ exhibited a lower intracellular uptake than that of LZ in A549 human lung cancer cells (in a two-dimensional culture). However, Bio-PEG-BSA showed significantly improved intracellular delivery as compared to that of PEG-BSA and BSA, probably because of favorable interactions with cells via biotin receptors. For A549/fibroblast coculture spheroids, PEG-LZ and PEG-BSA exhibited significantly decreased tissue penetration as compared with that of unmodified proteins. However, Bio-PEG-BSA showed tissue penetration comparable to that of unmodified BSA. In addition, citraconlyated LZ (Cit-LZ) showed reduced spheroid penetration as compared to that of LZ, probably owing to a decrease in protein charge. Taken together, chemical conjugation of targeting ligands-PEG to anionic proteins could be a promising strategy to improve intracellular delivery and in vivo activity, whereas modifications of cationic proteins should be more delicately designed.

• 천문학회보
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• 제46권1호
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• pp.56.2-56.2
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• 2021

Constraining the structure and thus the fate of giant molecular clouds (GMCs), the primary sites of star formation in galaxies, is crucial to understand the evolution of galaxies themselves. Exploiting the unprecedented sensitivity and angular resolution of the Atacama Large Millimeter/sub-millimeter Array (ALMA), we have measured the spatially-resolved (~ 20 pc resolution) properties of the GMCs in two nearby late-type galaxies, NGC 5806 (SAB(s)b) and NGC 6753 ((R)SA(r)b), as part of the WISDOM project. Although these results are preliminary, we identified ~ 200 resolved GMCs in NGC 5806 within a radius of 500 pc, most within a nuclear ring structure, and ~ 400 resolved GMCs in NGC 6753 within a radius of 2 kpc, most within a flocculent spiral structure. The GMCs of NGC 5806 have similar sizes but slightly higher linewidths than clouds in the Milky Way disc. Because the GMCs also have higher surface densities, the calculated cloud Virial parameters are nevertheless about unity, suggesting that the GMCs of NGC 5806 are in gravitational equilibrium and thus long lived. This is contrary to other WISDOM results on earlier-type galaxies, where large cloud linewidths are likely due to shear associated with the local (circular) orbital motions (rather than the clouds' self-gravity), and the clouds are either marginally or not gravitationally bound. These results support the notion that spheroids alter the dynamical states of clouds (morphological quenching), that are otherwise (i.e. in galaxy discs) fairly homogenous and similar to those of the Milky Way.

• KSBB Journal
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• 제16권1호
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• pp.24-29
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• 2001

급성 간부전 환자나 간이식 대기 상태의 환자의 생명을 간이 재생되거나 간 이식때 까지 연장시키기 위하여 생인공간이 많이 연구되고 있다. 본 연구에서는 적당한 cell opening을 가지는 폴리우레탄 폼을 15% NCO-prepolymer를 이용하여 직접 제조하였으며 이를 직경 3~4 mm 크기의 정육면체로 절단하여 충진형 반응기를 제조하였다. 분리된 일차간세포를 원심분리 방법을 이용하여 PUF 1 $cm^3$ 당 5.5$\pm$1.1$\times$$10^6$개의 세포를 접종하였으며 일주일간 관류 배양하면서 생인공간으로서의 성능을 측정하였다. 접종된 간세포는 구상체를 형성하였고 안정된 암모니아 제거능력과 요소 분비율을 나타내었다. 알부민 분비율은 배양 4일 이후 감소하는 경향을 보였다. 이상의 결과를 볼 때 PUF 충진형 간세포 반응기는 생인공간으로 개발되기에 충분한 가능성을 가진 것으로 판단된다.

• BMB Reports
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• 제51권10호
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• pp.514-519
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• 2018

Ovarian cancer is the most fatal gynecological malignancy in women and identification of new therapeutic targets is essential for the continued development of therapy for ovarian cancer. TRRAP (transformation/transcription domain-associated protein) is an adaptor protein and a component of histone acetyltransferase complex. The present study was undertaken to investigate the roles played by TRRAP in the proliferation and tumorigenicity of ovarian cancer stem cells. TRRAP expression was found to be up-regulated in the sphere cultures of A2780 ovarian cancer cells. Knockdown of TRRAP significantly decreased cell proliferation and the number of A2780 spheroids. In addition, TRRAP knockdown induced cell cycle arrest and increased apoptotic percentages of A2780 sphere cells. Notably, the mRNA levels of stemness-associated markers, that is, OCT4, SOX2, and NANOG, were suppressed in TRRAP-silenced A2780 sphere cells. In addition, TRRAP overexpression increased the mRNA level of NANOG and the transcriptional activity of NANOG promoter in these cells. Furthermore, TRRAP knockdown significantly reduced tumor growth in a murine xenograft transplantation model. Taken together, the findings of the present study suggest that TRRAP plays an important role in the regulation of the proliferation and stemness of ovarian cancer stem cells.

• The Korean Journal of Physiology and Pharmacology
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• 제22권5호
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• pp.555-566
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• 2018

Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) are used in tissue repair and regeneration; however, the mechanisms involved are not well understood. We investigated the hair growth-promoting effects of hUCB-MSCs treatment to determine whether hUCB-MSCs enhance the promotion of hair growth. Furthermore, we attempted to identify the factors responsible for hair growth. The effects of hUCB-MSCs on hair growth were investigated in vivo, and hUCB-MSCs advanced anagen onset and hair follicle neogeneration. We found that hUCB-MSCs co-culture increased the viability and up-regulated hair induction-related proteins of human dermal papilla cells (hDPCs) in vitro. A growth factor antibody array revealed that secretory factors from hUCB-MSCs are related to hair growth. Insulin-like growth factor binding protein-1 (IGFBP-1) and vascular endothelial growth factor (VEGF) were increased in co-culture medium. Finally, we found that IGFBP-1, through the co-localization of an IGF-1 and IGFBP-1, had positive effects on cell viability; VEGF secretion; expression of alkaline phosphatase (ALP), CD133, and ${\beta}-catenin$; and formation of hDPCs 3D spheroids. Taken together, these data suggest that hUCB-MSCs promote hair growth via a paracrine mechanism.

• 한국측량학회지
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• 제16권2호
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• pp.149-158
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• 1998

본 연구에서는 GPS에 의한 2차원 실용좌표의 획득면에 중점을 두고 WGS84와 벳셀타원체간의 3차원 좌표변환에 필수요소인 지역지오이드고 모델없이 두 타원체간 투영좌표의 상호 변환모델식을 구성하여 점검점에 적용하고 실용성과와 비교 고찰하므로서 UTM투영좌표에 의한 타원체간 2차원 좌표변환의 산정법을 제시한 것이다. 인천시지역을 시험모델로 관내 11개 삼각점으로부터 상호변환계수를 도출하고 점검점인 15개 지적삼각점에 적용하여 양방향의 변환정밀도를 분석한 결과, 측지좌표의 경우 위도 및 경도성분에서 각각 $\pm0.006$초, $\pm0.013$초, 평면직교좌 표의 경우는 남북 및 동서방향에서 각각 $\pm{17cm}$ 및 $\pm{30.0cm}$의 표준편차로 변환할 수 있었다. 또한, 남한지역을 대상으로 한 광역망, 경인.충남지역의 지역망 및 인천지역의 국소망으로부터 3-매개변환, 7-매개변환 및 UTM좌표간 변환계수를 각각 산출하여 점검점에 적용하고 변환넓이에 따른 변환기법별 변환특성도 고찰하였다.