• 대한간호학회지
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• 제50권3호
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• pp.487-500
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• 2020

Purpose: This study aimed to evaluate the validity and reliability of the Korean version of Menopause-Specific Quality of Life (MENQOL). Methods: The MENQOL was translated into Korean according to algorithm of linguistic validation process. A total of 308 menopausal women were recruited and assessed using the Korean version of MENQOL (MENQOL-K), the World Health Organization Quality of Life Brief Version (WHOQOL-BREF), and Center for Epidemiological Studies Depression Scale (CES-D-K). In estimating reliability, internal consistency reliability coefficients were calculated. Validity was evaluated through criterion validity and construct validity with confirmatory factor analyses using SPSS 23.0 and AMOS 25.0 software. Results: In item analyses, the "increased facial hair" symptom was excluded because of the low contribution of MENQOL-K. The confirmatory factor analysis supported good fit and reliable scores for MENQOL-K model, and the four-factor structure was validated (χ²=553.28, p<.001, NC=1.84, RMSEA=.05, AGIF=.85, AIC=765.28). The MENQOL-K consists of 28 items in 4 domains, including vasomotor (3 items), psychosocial (7 items), physical (15 items), and sexual subscales (3 items). There was an acceptable criterion validity with moderately significant correlation between MENQOL-K and WHOQOL-BREF. The Cronbach's α for the 4 subsacles ranged from .80 to .93. Conclusion: The MENQOL-K is a valid and reliable scale to measure condition-specific quality of life for perimenopausal and postmenopausal women. It can be used to assess the impact of menopausal symptoms on the quality of life of Korean women in clinical trials.

• 한국조경학회지
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• 제22권2호
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• pp.39-52
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• 1994

Existing natural resources were analyzed in regional scale in order to suggest natural park guidelines as a previous step of conservation plan. Results of both existing natural resources and land potentiality in preceding paper were combined to suggest comprehensive and qualitative method for understanding of natural land. Regional characteristics of natural park were, then, analyzed by this method, and specific planning guidelines were discussed. The existing natural resources were analyzed by the degree of location quotient, which were found by comparison of the grade of vegetation naturalness and the bio-physical land unit in preceding paper. Then, allowance of existing natural resources was identified, and importance of protection was described based on this result. The bio-physical land unit on Tokyo Province was found to be composed by smaller land units, which had a variety of relationship with grade of vegetation naturalness: from the specific(1st) to many stages(5th). This meant a characteric of each land unit. By this combined approach, the characteristics and spatial distribution of natural land were discussed in the regional scale, and characteristic of nateral park were analyzed in terms of natural location. National park were located in the land unit where both land capability and importance of portection were the 6th. Quasi national park and 1 province natural park were located in the land unit where land capability was the 6th and importance of protection were the 5th. Seven province natural parks were located in the land unites were both criteria under the 5th were mixed. based on the results of this study, the followings were suggested for specific planning guidelines in the case of Takao quasi nation park: (a) conservative land use within the limit of carrying capacity of this area by the conservation of biotic and abiotic natural resources, (b) conservation of both natural and semi-natural vegetation, (c) utilization of this area as a urban forest.

• 한국분말재료학회지
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• 제16권3호
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• pp.185-190
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• 2009

Fe-Cr steels are the most promising candidate for interconnect in solid oxide fuel cells. In this study, an effective, dense and well adherent (LaSr)(CrCo)$O_3$ [LSCC] coating layer was produced onto 430 stainless steel (STS-430) by atmospheric plasma spraying and the oxidation behavior as well as electrical properties of the LSCC coated STS-430 were investigated. A significant oxidation of pristine STS-430 occurred at $800^{\circ}C$ in air environment, leading to the formation of $Cr_2O_3$ and $FeCr_2O_4$ scale layer up to ${\sim}7{\mu}m$ after 1200h, and consequently increased an area specific resistance of $330\;m{\Omega}{\cdot}cm^2$. Although the plasma sprayed LSCC coating contained the characteristic pore network, the coated samples presented apparent advantages in reducing oxidation growth of STS-430, resulting a decrease in oxide scale thickness of ${\sim}1{\mu}m$ at $800^{\circ}C$ after 1200h. The area specific resistance of the LSCC coated STS-430 was much reduced to ${\sim}7\;m{\Omega}{\cdot}cm^2$ after exposure at $800^{\circ}C$ for 1200h, compared to that of the pristine STS-403.

• Journal of Microbiology and Biotechnology
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• 제14권2호
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• pp.284-289
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• 2004

A PCR assay for the rapid detection of Vibrio vulnificus strains was developed using a virulence gene for elastase found in various Vibrio species. The DNA sequences in the elastase gene facilitated the identification of a species-specific probe for pathogenic V. vulnificus strains from both clinical and environmental sources. Using an elastase gene-based PCR reaction, a species-specific 507-bp PCR product was visualized by agarose gel electrophoresis. Three different DNA extraction methods were then compared to improve the simplicity and rapidity of detection. A PCR assay using the conventional DNA extraction or boiling method was able to detect as few as 25 V. vulnificus cells, making the detection limits at least 1-log-scale lower than that for the EDT A-treated DNA extraction method. In particular, the boiling method, which does not require purification of the chromosomal DNA, was very effective in terms of simple and rapid detection. Meanwhile, the detection limit in a mixed bacterial culture that included other bacteria, such as Escherichia coli or Bacillus subtilis, was two V. vulnificus cells, which was 1-log-scale lower than that for the control. Accordingly, when coupled with a new DNA extraction method, the elastase gene-based PCR can provide a rapid, specific, and sensitive method for identifying V. vulnificus in clinical and environmental samples.

• 한국물환경학회지
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• 제32권2호
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• pp.149-162
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• 2016

In recent years, there has been demand for precise estimations of pollutant loads on nationwide scale for the development of appropriate site specific (watershed specific) policies to reduce the negative impact of pollutant loads. River flow data and water quality data that were previously collected by various research institutes and universities for specific research purposes for a limited period was utilized in this study. However, only TMDL 8-day interval flow and water quality data were available in national scale. Three watersheds were selected and pollutant loads were calculated by two methods i.e., Numeric Integration (NI) method and Soil and Water Assessment Tool (SWAT). Subsequently, the results were compared to determine the appropriate method for monitoring nonpoint source networks nationwide. The SWAT model was calibrated and its estimated daily flow data were used in the NI method with estimated sediment data for 8-day monitoring data for three watersheds. The results indicated that the quantity of pollutant loads estimated with the NI and SWAT are different to some degrees especially during the summer season for all the three study watersheds. Thus, more frequent sampling of water quality is needed for nonpoint source pollutant estimation.

• 대한불안의학회지
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• 제15권2호
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• pp.122-126
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• 2019

Objective : Dynamic proteolysis, through the ubiquitin-proteasome system, is an important molecular mechanism for the constant regulation of synaptic plasticity and stress responses in humans. In this study, we examined whether genetic variants in the ubiquitin-specific peptidase (USP) genes were associated with psychological traits of resilience and susceptibility to neuropsychiatric disorders for each gender. Methods : A total of 344 Korean healthy youths (190 males, 154 females) were included in the study. A genotyping of rs2241646 of USP2 and rs346006 of USP46 was performed. The Connor-Davidson Resilience Scale and Brief Fear of Negative Evaluation Scale were administered for measuring trait resilience and social anxiety, respectively. The genetic associations of the USP variants were tested using multiple analyses of covariance with psychological traits as dependent variables after controlling for age in each gender. Results : For USP2 rs2241646, women with the TT genotype showed significantly higher resilience and lower social anxiety, as compared to those carrying the C allele. There were no associations between USP46 rs346005 and the psychological traits in both genders. Conclusions : The present study showed a possible genetic association between the USP2 rs2241646 and stress resilience and trait anxiety in women. The findings suggest that ubiquitin-proteasome system may be related to the resilience and susceptibility to stress-related neuropsychiatric disorders such as anxiety disorders, possibly through the regulation of dynamic proteolysis responses to stress.

• 국제물리치료학회지
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• 제10권2호
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• pp.1803-1809
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• 2019

Background: Cervical mobilization has been applied mainly for the improvement of arm and neck movements and pain reduction, and little research has been done to improve the executive function. Since this kind of so-called mechanical neck pain is one of most common symptoms, there are controversial issues about this with spine alignment. Posteroanterior (PA) mobilization from the Maitland concept is a process of examination, assessment, and treatment of neuromusculoskeletal disorder by manipulative physical therapy. Objective: To examine the short-term benefits of mobilization for patients with non-specific neck pain. Design: Dual-group Pretest-Posttest Design from the Quasi-Experimental research Methods: Fourteen participants (male 8, female 6; 20's of their age) with non-specific neck pains which are distributed all the unilateral or bilateral body side were recruited. Participants were categorized to Neck Pain with Movement Coordination Impairments (NPMCI) and Neck Pain with Mobility Deficits (NPMD) groups according to the results of physical examination. Professional physical therapist who has over 15-years-of clinical experience applicated manipulative therapy for the neck pain, an occupational therapist only conducted evaluations; K-NDI (Korean version of the Neck Disability Index), VAS (Visual Analog Scale), BDS-K (Korean version of Behavioral Dyscontrol Scale) for decreasing possible adverse effects; there were no person who reported other symptoms followed 4 weeks from the trial. Results: In the NPMCI group, data analysis indicated statistical differences between the PA mobilization interventions in NDI and BDS-K; even though, pain was reduced in VAS, this is not a significantly differ. In the NPMD group, data analysis represented statistical differences between the PA mobilization interventions in NDI, VAS and BDS-K; the scores were represented to be increased or the pain got relief. Conclusions: PA mobilization techniques according to Maitland concept have beneficial effects in patients with neck pain and other clinical positive effects which included neck disability, pain itself and motor function of upper extremity.

• Genomics & Informatics
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• 제21권2호
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• pp.19.1-19.13
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• 2023

Liver cancer, particularly hepatocellular carcinoma (HCC), poses a significant global threat to human lives. To advance the development of innovative diagnostic and treatment approaches, it is essential to examine the hidden features of HCC, particularly its 3D genome architecture, which is not well understood. In this study, we investigated the 3D genome organization of four HCC cell lines-Hep3B, Huh1, Huh7, and SNU449-using in situ Hi-C and assay for transposase-accessible chromatin sequencing. Our findings revealed that HCC cell lines had more long-range interactions, both intra-and interchromosomal, compared to human mammary epithelial cells (HMECs). Unexpectedly, HCC cell lines displayed cell line-specific compartmental modifications at the megabase (Mb) scale, which could potentially be leveraged in determining HCC subtypes. At the sub-Mb scale, we observed decreases in intra-TAD (topologically associated domain) interactions and chromatin loops in HCC cell lines compared to HMECs. Lastly, we discovered a correlation between gene expression and the 3D chromatin architecture of SLC8A1, which encodes a sodium-calcium antiporter whose modulation is known to induce apoptosis by comparison between HCC cell lines and HMECs. Our findings suggest that HCC cell lines have a distinct 3D genome organization that is different from those of normal and other cancer cells based on the analysis of compartments, TADs, and chromatin loops. Overall, we take this as evidence that genome organization plays a crucial role in cancer phenotype determination. Further exploration of epigenetics in HCC will help us to better understand specific gene regulation mechanisms and uncover novel targets for cancer treatment.

• 감성과학
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• 제21권3호
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• pp.103-114
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• 2018

SPANE(the Scale of Positive and Negative Experience) 척도는 기존 정서 측정도구의 한계를 보완하기 위해 Diener 등(2010)이 개발한 정서적 안녕감 척도이다. 이 척도는 긍정적 정서와 부정적 정서 경험을 측정하는 총 12문항으로 구성되어 있으며, 포괄적 정서(e.g. positive, negative)와 구체적 정서(e.g. joyful, sad) 문항이 포함되어 있다. 각성수준의 측면에서도 고(e.g. joy, angry), 중(e.g. happy, afraid), 저(e.g. contented, sad) 각성 정서들이 모두 반영되어 있다. 본 연구는 한국판 SPANE 척도의 요인구조 및 성별 측정 동일성을 검토하였다. 이를 위해서, 한국 대학생 총 551명(남자 276, 여자 275)의 자료를 분석했다. 그 결과, SPANE 척도는 양호한 수준의 신뢰도와 요인 타당도를 보였다. 또한 남녀 집단 간에 요인구조, 요인부하량, 절편이 동일한 것으로 나타나서, 성별 척도 동일성이 확인되었다. 이러한 결과는 SPANE 척도가 한국에서도 대학생들의 정서적 안녕감을 측정하기에 적합한 척도일 뿐 아니라 성별 차이를 비교하는 타당한 도구가 될 수 있음을 시사한다.

• 농촌계획
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• 제19권1호
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• pp.109-122
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• 2013

The rapid rural urban migration and aging has generated an over-depopulation problems in rural areas since the 1980s. The purpose of this study constructs the marginal size of rural over-depopulated village through the analysis of the residential disparities such as farmer's ratio, basic life service accessibility, and levels of social and economic factors for each village community. This marginal scale could support evaluating diverse rural policies, which have been planned to apply to the rural development programs at the village level. The major challenges for over-depopulated villages are the lack of basic facilities, production infrastructures and inactive communities in the village. Therefore, the quantitative analysis of rural residential disparities according to rural village scale can provide the criteria for rural over-depopulated villages. We utilized Korea Agricultural Survey Data(2010) including specific residential condition of village level. The present study adopt multinomial-logit model for quantitative analysis of different village scales and decomposition techniques to separate the direct effect by the village scale factor from the endowment effects by regional or area characteristics, and residual effect by unknown factors. The present study found that the minimum scale of a rural over-depopulated village was 40 and 60 houses for the respective conditions of farmer's ratios less than 50% and greater than 75%. It was concluded based on the study findings that threshold scale could support evaluating the diverse rural policies, which have been planned to apply to the rural development programs at the village level.