• Korean Journal of Environment and Ecology
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• v.34 no.4
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• pp.334-344
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• 2020

A total of 24 families, 44 species, and 658 benthic macroinvertebrates were identified, and Ecdyonurus dracon Kluge (13%) was the dominant species in forested streams within the Yeonyeopsan (Mt.). A total of four habit categories (i.e., clingers (56%), burrowers (19%), swimmers (14%), and sprawlers (56%)) were identified, and clingers were the dominant habit at all survey points except point one (UP1). Habitat characteristics were depended on the hydraulic factors (e.g., flow velocity, depth, and substrates), water quality (e.g., DO and water temperature), and the habitat characteristics were differed in the riffle, which has a faster the flow velocity, compared by in the stagnant pool. In other words, in riffles, the clingers dominated in high flow velocity with the large maximum and median grain size for substrates in the habitats regardless of depth, but the burrowers and sprawlers were dominant in low flow velocity with the small maximum and median grain size for substrates in the habitats. Moreover, DO and flow velocity were in positive correlation (y = 0.6666x - 0.659, R² = 0.0851), and the habitat for burrowers was wider than that for sprawlers or clingers. The water depth was negatively correlated with water temperature (y = -26.397x + 283.87, R² = 0.1802) since the water temperature is more sensitive to insolation in shallow depth. pH was positively correlated with water temperature. The investigation of the habitat characteristics by separating the relations between pH and DO in upstream and downstream showed the low pH and high DO in the upstream with a high crown density of 68%, regardless of community composition. On the other hand, high pH and low DO in the downstream with a relatively low crown density of 51%. It was considered that the riparian forest played a role in suppressing the growth of attached algae and the controlling water temperature in headwater streams. Our findings identified the habitat characteristics of benthic macroinvertebrates in a headwater stream. We expected that the finding can provide reference data for suggesting conservation and management plans in a headwater stream and increasing academic value.

• Journal of Plant Biotechnology
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• v.41 no.4
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• pp.194-200
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• 2014

Monodehydroascorbate reductase (MDHAR) is an important enzyme that plays a role in the detoxification of reactive oxygen species (ROS) by maintaining reduced pool of ascorbate through recycling the oxidized form of ascorbate. In this study, we isolated a PagMDHAR1 gene from Populus alba ${\times}$ P. glandulosa, and investigated its expression characteristics. The PagMDHAR1 cDNA encodes a putative 434 amino acids containing FAD- and NAD(P)H-binding domains. Southern blot analysis indicated that a single nuclear gene encodes this enzyme. Northern hybridization analysis revealed that PagMDHAR1 is highly expressed in both suspension cells and flower tissues, while its expression levels were enhanced by drought, salt, cold, wounding and ABA. Therefore, PagMDHAR1 might be expressed in response to abiotic stress through the ABA-mediated signaling pathway in this poplar species, suggesting that the PagMDHAR1 plays an important role in the defense mechanisms against oxidative stress.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.3
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• pp.336-343
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• 2019

In order to investigate the density of seasonal incidence of mosquitoes, a vector of infectious diseases in Jeju region, this study collected mosquitoes using a Black light trap (BL) and Biogents' Sentinel 2 Mosquito Trap (BG), dividing the region into cattle sheds, habitats for migratory birds, and the downtown area, twice a month for 9 months from March through November 2018. Then, this study conducted identification and classification and checked for the presence of Flavivirus using reverse transcription polymerase chain reaction (RT-PCR). As for the mosquito population, 1,847 mosquitoes (six genera, 12 species) were collected. The places where most mosquitoes were collected were copses near craft workshops in habitats for migratory birds and Jungang-dong in the Seogwipo downtown area. For the population, Culex pipiens pallens was the dominant species (76.9%), followed by Aedes albopictus (8.9%). Most of the population was collected in June, followed by August and October. This study conducted a RT-PCR test with 1,847 collected mosquitoes, which were divided into 50 pools if they had Flavivirus. All turned out to be negative. However, the results of the investigation show the presence of Culex tritaineniorhychus, Aedes albopictus, and Anopheles sinensis and can be used as a basis for the comprehensive prevention management of mosquitoes.

• Ecology and Resilient Infrastructure
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• v.4 no.3
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• pp.169-179
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• 2017

This study analyzed the aspect of bed change according to the stable channel design on the Wonju River to quantitatively evaluate habitat suitability (HS). According to the result of evaluating stable channel of object section in the Wonju River, 17 sections among total 20 sections were stable and 3 sections were unstable. Physical disturbance improvement evaluation (PDIE) was the range average showed good disturbance condition with a range average of 112.17 points. Habitat suitability index of Zacco Koreanus, the most dominant species of the Wonju River, was used for analysis of physical habitat for fish. According to the physical habitat analysis result, HS was 0.16 and weighted usable area (WUA) was $347.68m^2$. The methods of improving/introducing/removing structures and dredging stream channel were used for stable channel design of unstable channel, and analyzed PDIE according to the aspect of bed change and changes in habitat suitability. Stable channel design was possible in 19 sections in times of structures improvement/introduction/removal, and PDIE was 117.53 points, HS was 0.14 points, and WUA was $313.37m^2$. Stable channel design was possible in all 20 sections when dredging the stream channel. PDIE was 116.50 points, HS was 0.16, and WUA was $332.14m^2$. Therefore, this study obtained channel design measures that can improve physical soundness and stability of the Wonju River, and it was analyzed that it will have no impact on changes of physical disturbance and physical habitat. Furthermore, this study analyzed velocity and depth of each section and appearance frequency of riffle and pool to analyze correlation between physical disturbance and physical habitat. According to the analysis result, it was identified that the analysis of riffle and pool showed similar result as the evaluation result of physical habitat.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.101-110
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• 2015

Parameters of mating system and pollen flow of a Pinus densiflora population in Buan, South Korea, were estimated using seven nuclear microsatellite markers. The expected heterozygosity ($H_e$) was 0.614 in mother trees and 0.624 in seeds. Fixation index (F) was 0.018 and 0.087 in each generation. There was no significant genetic difference between the generations (P > 0.05). From MLTR, the outcrossing rate ($t_m$), the biparental inbreeding ($t_m-t_s$), and the correlation of paternity ($r_p$) were 0.967, 0.057, and 0.012, respectively. tm was larger but $t_m-t_s$ and $r_p$ were smaller than those of allozyme markers in Pinus densiflora. These values were similar to those of microsatellite markers in other pine species. The optimal pollen dispersal model from TwoGener was the normal dispersal model with the effective density of 220 trees/ha and its level of genetic differentiation in pollen pool structure (${\Phi}_{ft}$) was 0.021. The average radial distance of pollen flow (${\delta}$) was calculated as 11.42 m, but no correlation between the pairwise-${\Phi}_{ft}$ and the geographical distance among mother trees was at Mantel test (r = -0.141, P > 0.05). Although the effective pollen dispersal in the population seems to be restricted, the amount of genetic variation might be maintained in each generation without a loss of genetic diversity. It might be because the genetic diversity in pollen pool was high but the genetic difference between pollen donors was small under the complete random mating condition in the Pinus densiflora population in Buan.

• The Korean Journal of Malacology
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• v.31 no.4
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• pp.307-314
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• 2015

This study sampled endangered species, Lamprotula coreana, and surveyed its habitat at the Guem River with three times from June to August 2013. To assess the habitat, this study conducted field survey considering diverse physical conditions of stream, such as pool, run and riffle, and measured transect, water depth, water velocity, substrate structure, and habitat type. When L. coreana collected, length, weight and age were measured. Water velocity, water depth and substrate structure were recorded to develop HSI (habitat suitability index) and performed PHABSIM (physical habitat simulation) to estimate the optimum flow discharge. Water level, flow discharge and transect data were used for habitat assessment, and PHABSIM was applied to calculate WUA (weighed usable area). Shell length was $73.1{\pm}18.4mm$ (28.5-102.0 mm), shell weight was $131.6{\pm}72.3g$ (28.0-281.0 g) and age was two to seven years from L. coreana collected at the upstream of the Guem River. Developed HSI indicated that the optimal habitat for L. coreana was 0.4-0.5 m for water depth, 0.3-0.5 m/s for water velocity and sand to boulder for substrate structure. The optimum ecological flow discharge for L. coreana was 2.1 cms and WUA was $3,730m^2$/1000 m by the result of PHABSIM. Recently, river construction work and habitat disturbance have caused negative impact on the distribution of L. coreana. The result of this study would provide fundamental data for habitat restoration and management of L. coreana.

• Biomolecules & Therapeutics
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• v.1 no.2
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• pp.188-195
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• 1993

In order to understand the machanism of action and regulation of ${\beta}$-adrenergic receptor in terms of molecular level, the purification of receptor protein has a fundamental importance. Moreover, species differences among avian, amphibian and mammalian ${\beta}$-adrenergic receptors make it more important to purify mammalian ${\beta}$-adrenergic receptor. Because ${\beta}$-adrenergic receptor is an integral membrane protein, it must be solubilized from the membrane for the purification. The purpose of the present study was to solubilize and characterize the mammalian $\beta$-adrenergic receptor from guinea pig lung in quantities by more efficient and practical method eventually to purify receptor. Guinea pig lung membrane preparation was solubilized by sequential treatment of buffers containing low and high concentration of digitonin which are 0.2 and 1.2% respectively. About 50% of the total receptor pool was released by this double extraction procedure. The $\beta$-adrenoceptors in the digitonin extract were identified using the ${\beta}$-adrenergic antagonist, (-)-[$^3H$]-dihydroalprenolol ([$^3H$]DHA). The solubilized receptor retained all of the essential characteristics of membrane-bound receptor, namely saturability; stereoselectivity; high affinity to ${\beta}$-adrenergic drugs. For the measurement of soluble receptor activity, Sephadex G-50 chromatography method has been widely used. Inspite of its accuracy and wide acceptance, this technique employed troublesome column work which required long time to assay the activity of receptor. We employed another methods to measure receptor activity. When using 0.5% polyethylenimine pretreated GF/B glass fiber filter, filtration technique could be used to measure soluble receptor activity. This technique enabled us to reduce the total amount of time to assay by a factor of 4 as well as to detect soluble receptor. In the present study, we could establish more efficient and practical solubilization method of mammalian $\beta$-adrenergic receptor. The rapidity and high yield of this solubilization scheme, together with the favorable recovery of the receptor activity, are significant steps toward the ultimate purification of the mammalian $\beta$-adrenergic receptor. The result of this study together with more convenient purification method could provide large amount of purified receptor with ease for various research purposes.

• Journal of Ecology and Environment
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• v.43 no.2
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• pp.161-182
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• 2019

Quantifying the amount of carbon pools in forest ecosystems enables to understand about various carbon pools in the forest ecosystem. Therefore, this study was conducted in the Chilimo dry afromontane forest to estimate the amount of carbon stored. The natural forest was stratified into three forest patches based on species composition, diversity, and structure. A total of 50 permanent sample plots of 20 m × 20 m (400 ㎡ ) each were established, laid out on transects of altitudinal gradients with a distance of 100 m between plots. The plots were measured twice in 2012 and 2017. Tree, deadwood, mineral soil, forest floor, and stump data were collected in the main plots, while shrubs, saplings, herbaceous plants, and seedling data were sampled inside subplots. Soil organic carbon (SOC %) was analyzed following Walkely, while Black's procedure and bulk density were estimated following the procedure of Blake (Methods of soil analysis, 1965). Aboveground biomass was calculated using the equation of Chave et al. (Glob Chang Biol_20:3177-3190, 2014). Data analysis was made using RStudio software. To analyze equality of means, we used ANOVA for multiple comparisons among elevation classes at α = 0.05. The aboveground carbon of the natural forest ranged from 148.30 ± 115.02 for high altitude to 100.14 ± 39.93 for middle altitude, was highest at 151.35 ± 108.98 t C ha^-1 for gentle slope, and was lowest at 88.01 ± 49.72 t C ha^-1 for middle slope. The mean stump carbon density 2.33 ± 1.64 t C ha^-1 was the highest for the middle slope, and 1.68 ± 1.21 t C ha^-1 was the lowest for the steep slope range. The highest 1.44 ± 2.21 t C ha^-1 deadwood carbon density was found under the middle slope range, and the lowest 0.21 ± 0.20 t C ha^-1 was found under the lowest slope range. The SOCD up to 1 m depth was highest at 295.96 ± 80.45 t C ha^-1 under the middle altitudinal gradient; however, it was lowest at 206.40 ± 65.59 t C ha^-1 under the lower altitudinal gradient. The mean ecosystem carbon stock density of the sampled plots in natural forests ranged from 221.89 to 819.44 t C ha^-1. There was a temporal variation in carbon pools along environmental and social factors. The highest carbon pool was contributed by SOC. We recommend forest carbon-related awareness creation for local people, and promotion of the local knowledge can be regarded as a possible option for sustainable forest management.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.12
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• pp.3748-3754
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• 2009

An integral head loss test in a test apparatus was conducted to simulate chemical effects on a head loss across a strainer in a pressurized water reactor (PWR) containment water pool after a loss of coolant accident (LOCA). The test was conducted during 30 days in the condition of a short spray, a long spray, and no materials with chemical effects. The result exhibited that the head loss was affected on amounts of the exposed materials according to spray conditions. XRD analysis of the collected precipitates showed that the precipitates were phosphate compounds. Comparison of the head loss with dissolved species concentration showed that high increase rate of the head loss resulted from the corrosion of aluminum and zinc but slow increase rate of the head loss resulted from the precipitates induced by Si, Mg, and Ca from leaching reaction at NUKON and concrete after passivation of metal specimens.

• Journal of Korean Society of Forest Science
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• v.103 no.2
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• pp.264-269
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• 2014

The aim of this study was to determine the current distribution area of Robinia pseudoacacia habitat and to estimate its stand yield as well as its carbon stocks. In order to do so, the area of R. pseudoacacia distribution is obtained based on the large-scaled forest type map (1:5,000). Also, Weibull diameter distribution model is used to predict the yield of R. pseudoacacia stands. In addition, carbon emission factor is applied to calculate carbon stocks and removals. To obtain the stand yield of R. pseudoacacia, we developed estimation equation considering growth factors of the stand, e.g. mean diameter, the basal area, maximum and minimun diameter and etc. and tested it to ensure accuracy. Consequently, estimation equation derived from all growth factors have shown significance that could also be used for analysis. Site index was also established to determine the productivity of the forestland that later turned out to be ranging from 16 to 22. Based on these results, stand yield tables were drawn up. R. pseudoacacia is widely distributed in inland areas of Gyeongsang, Chungcheong and Gyeonggi provinces which covers total area of 26,770 ha. And when it is converted into carbon stocks, it amounts to 2,517,598tC with annual carbon uptake of 3.76tC/ha which is comparable to Querqus species that is known to storer large amounts of carbon. Therefore, R. pseudoacacia is also expected to serve as a viable carbon pool that would contribute to the mitigation of climate change. Furthermore, stand yield tables, an outcome of this survey would assist not only in proper management but also in sustainable management policy of R. pseudoacacia.