• 한국어류학회지
• /
• 제33권4호
• /
• pp.205-216
• /
• 2021

한 유전좌위 또는 소수의 유전좌위에 기반한 계통발생학적 재구성은 분자 계통수/종 계통수의 불일치로 인해 오해를 불러일으킬 수 있다. 종의 구분과 종내 연구에서도 적은 유전좌위를 사용할 때 통계력 부족으로 해상도가 낮은 경우가 많이 발생한다. 엑손 포획법은 게놈 규모의 데이터를 수집하는 가장 효율적인 방법 중 하나로, 종내 및 상위 수준에서 생물의 패턴과 역사를 구명하는 연구에 크게 이바지할 수 있다. 이 논문에서는 단일 유전자 방법에서 게놈 접근으로의 전환의 진보와 게놈 기술에 비해 엑손 포획법의 적용 이점을 설명하였다. 또한 엑손 포획법의 원리를 설명하고 이 방법의 적용을 위한 상세한 제언을 기술하였다. 최종적으로, 두 가지 적용을 활용한 엑손 포획법을 설명하고 이 기술에 대한 미래 전망을 논의하였다.

• Journal of Ecology and Environment
• /
• 제37권4호
• /
• pp.395-410
• /
• 2014

The genus Callophyllis is recorded as six separate species with imprecise species delimitation in Korea. To elucidate the species boundaries of Korean Callophyllis, we performed morphological observations and molecular analyses, and included three Japanese Callophyllis species from the type locality. From the results of molecular analyses using plastid rbcL and mitochondrial COI-5P genes, we confirmed ten Callophyllis species, including five cryptic ones: C. adhaerens, C. adnata, C. crispata, and C. japonica from Korea and Japan; C. hayamensis as an unrecorded species from Korea; C. cartilaginea, C. mollitia, C. repens, C. serratifolia, and C. undulata as new species from Korea. There were no Korean specimens that matched C. adnata or C. crispata from Japan, except Korean C. japonica, which formed a genetic group with the Japanese species. We obtained the interspecific divergences among the five cryptic species as 0.6-4.5% in rbcL and 2.8-8.4% in COI-5P. We recognized that the species diversity of Callophyllis has been underestimated from the northwestern Pacific region. The species boundary of Callophyllis from Korea and Japan will be a cornerstone to revealing the phylogenetic affinity of the genus distributed in both hemispheres of the western Pacific.

• ALGAE
• /
• 제27권4호
• /
• pp.249-258
• /
• 2012

Nongeniculate coralline red algae are a common element of the Brazilian coastal zone, especially associated to coral reefs. During the course of ecological studies at Parque Municipal Marinho do Recife de Fora, two species of non-geniculate Corallinales were the major organisms covering the reef. Analyses of the vegetative and reproductive features of the species were analyzed; indicating that one new species of the genus Lithothamnion is proposed here based on the combination of several features associated with anatomy of the tetrasporangial conceptacles in relation to other species of the genus for which modern accounts are available. This new proposal along with other new species, new combinations and range extension of some species of the genus based in similar features clearly suggest that stability in species delimitation is possible. The second species found Pneophyllum conicum represents a range extension of more than 6,000 km from the Pacific in to the Atlantic Ocean suggesting that some nongeniculate species are widely distributed. The occurrence and abundance of these species supports and emphasizes the need for an extensive taxonomic reassessment of coralline red algae in the context of Brazilian coral reef biodiversity.

• 식물분류학회지
• /
• 제50권1호
• /
• pp.22-26
• /
• 2020

Chrysosplenium aureobracteatum Y. I. Kim & Y. D. Kim (Saxifragaceae) is a recently described endemic species growing in the central part of the Korean peninsula. It requires constant monitoring for conservation due to its limited distributions. There is also a need for molecular markers for proper assessments of the genetic differentiation of C. aureobracteatum from species morphologically similar to it. In this study, we developed microsatellite markers that can be used to evaluate the genetic diversity of this species, representing fundamental data with which to conserve the natural populations of the species. A total of 17 expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed by the Illumina pair-end sequencing of the transcriptomes of C. aureobracteatum. These markers were successfully applied to populations of C. aureobracteatum and to its most closely related species, C. barbatum, revealing high polymorphism in both species. The EST-SSR markers developed in this study were proven to be useful not only to monitor the population genetic structure of C. aureobracteatum for conservation purposes but also to study the genetic delimitation of the species from species closely related to it.

• ALGAE
• /
• 제34권4호
• /
• pp.253-266
• /
• 2019

Several species classified to the genus Ulva are primarily responsible for causing green tides all over the world. For almost two decades, green tides have been resulted in numerous ecological problems along the eastern coast of Jeju Island, Korea. In order to characterize the species of Ulva responsible for causing the massive blooms on Jeju Island, we conducted DNA barcoding of tufA and rbcL sequences on 183 specimens of Ulva from eight sites on Jeju Island. The concatenated analysis identified five bloom-forming species: U. australis, U. lactuca, U. laetevirens, U. ohnoi and a novel species, U. pseudo-ohnoi sp. nov. Among them, U. australis, U. lactuca, and U. laetevirens caused to the blooms coming mainly from the substratum. U. ohnoi and U. pseudo-ohnoi sp. nov. were causative the free-floating blooms. Four species, except U. australis, are characterized by marginal teeth. A novel species, U. pseudo-ohnoi sp. nov., is clearly diverged from the U. lactuca, U. laetevirens, and U. ohnoi clade in the concatenated maximum likelihood analysis. Accurate species delimitation will contribute to a management of massive Ulva blooms based on this more comprehensive knowledge.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2020년도 춘계학술대회
• /
• pp.36-36
• /
• 2020

Symplocos Jacq. including about 350 species is the sole isolated genus of the family Symplocaceae. Despite poorly documented species delimitation and unresolved taxonomic nomenclature four species of Symplocos (S. coreana, S purnifolia, S sawafutagi, and S. tanakana) have been described in Korea. In this study, we carried the comparative wood anatomy of all the four species of Korean Symplocos to understand the wood anatomical variations within these four species. The result of this study indicated that Korean Symplocos are comparatively indistinguishable in terms of their qualitative wood features except for exclusively uniseriate rays present in S. purnifolia instead of uni- to- multiseriate in other three species. However, discrepancies are observed in quantitative wood variables such as vessel density, vessel size, and ray density. The vessel density of S. purnifolia (highest among the four species) is more than two times higher than the S. sawafutagi (lowest among the four species) and S. tanakana. On the other hand, vessel size is likewise reverse to the vessel number relationships i. e. vessel circumference and diameter in both planes of S. sawafutagi and S. tanakana is almost twice a larger than S. purnifolia. Interestingly, S. coreana remains in between of these two groups in terms of vessel features and closer to S. purnifolia in terms of ray density. The cluster analysis based on the paired group (UPGMA) algorithm using the Euclidean similarity index clearly differentiates S. purnifolia from the rest of the taxa representing the first isolated clade of the tree.

• Animal Systematics, Evolution and Diversity
• /
• 제28권4호
• /
• pp.269-278
• /
• 2012

Gobiids are hyperdiverse compared with other teleost groups, with about 2,000 species occurring in marine, freshwater, and blackish habitats, and they show a remarkable variety of morphologies and ecology. Testing the effectiveness of DNA barcodes on species that have emerged as a result of radiation remains a major challenge in evolutionary biology. Here, we used the cytochrome c oxidase subunit 1 (COI) sequences from 144 species of gobies and related species to evaluate the performance of distance-based DNA barcoding and to conduct a phylogenetic analysis. The average intra-genus genetic distance was considerably higher than that obtained in previous studies. Additionally, the interspecific divergence at higher taxonomic levels was not significantly different from that at the intragenus level, suggesting that congeneric gobies possess substantial interspecific sequence divergence in their COI gene. However, levels of intragenus divergence varied greatly among genera, and we do not provide sufficient evidence for using COI for cryptic species delimitation. Significantly more nucleotide changes were observed at the third codon position than that at the first and the second codons, revealing that extensive variation in COI reflects synonymous changes and little protein level variation. Despite clear signatures in several genera, the COI sequences did resolve genealogical relationships in the phylogenetic analysis well. Our results support the validity of COI barcoding for gobiid species identification, but the utilization of more gene regions will assist to offer a more robust gobiid species phylogeny.

• 식물분류학회지
• /
• 제45권4호
• /
• pp.306-317
• /
• 2015

본 연구에서는 간혹 흰바위취(Micranthes manchuriensis)와 동일종으로 취급되기도 하는 한국 고유종 구실바위취(Saxifraga octopetala)의 분류학적 지위를 알아보고자 수행되었다. 구실바위취의 계통학적 위치와 종의 경계를 확인하기 위해 두 종의 기준표본에 대한 형태적 검토와 핵 리보솜 DNA의 ITS 지역 염기서열에 대한 집중적인 계통분석을 시행하였다. 총 65개 구실바위취 개체는 ITS 계통수에서 하나의 무리를 이루면서 Micranthes 분기군(clade)에 포함되었고, 톱바위취 및 흰바위취와 가까운 계통 유연관계를 나타내었다. 중국과 러시아에서 채집된 다수의 흰바위취 개체 역시 독자적인 분기군을 형성하면서 M. nelsoniana var. pacifica 및 M. fusca와 자매군을 이루었다. 구실바위취의 실체가 모호했던 것은 흰바위취 개체와 톱바위취의 화서를 함께 포함하고 있는 복합표본인 Wilford의 채집품(흰바위취의 기준표본)을 Nakai가 잘못 관찰하였기 때문으로 생각되었다. 구실바위취와 흰바위취의 높은 형태적 유사성에도 불구하고 이들은 지하 포복경의 특징에 있어서 차이를 보였다. ITS 계통수에서 구실바위취가 Micranthes 내에 자리하는 것으로 나타나 구실바위취는 Micranthes 내에서 종의 지위를 유지하여야 할 것으로 판단되었고, 이에 따라 신조합명(Micranthes octopetala)을 발표하였다.

• Journal of Ecology and Environment
• /
• 제36권4호
• /
• pp.449-463
• /
• 2013

DNA barcoding is becoming a widely applied tool to accurately discriminate red algae. We tested the effectiveness of DNA barcoding for identification and discovery of Champia species in Korea and clarified the phylogenetic relationships using the plastid rbcL gene. As results, we described four species of Champia such as C. inkyua sp. nov., C. recta Noda, C. bifida Okamura, and C. expansa Yendo. A new species, C. inkyua, is characterized by entangled thallus, terete and irregular branches, hooked apices, and longitudinal filaments running throughout the frond periphery only. Longitudinal filaments were composed of a complete cell with two half cells between diaphragms in the cavity. C. recta and C. bifida were reinstated with previously used names of C. parvula and C. compressa, respectively. C. recta is the first recorded species from Korea and is characterized by an erect thallus, terete and irregular branches, and straight apices. C. bifida is characterized by compressed thallus, pinnate or alternate branches, and bifid apices. C. expansa is characterized by flabellate thallus and dichotomous branches. Molecular analyses of COI and rbcL genes revealed sufficient sequence divergence to warrant species recognition in the genus Champia.