Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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Development of EST-SSR markers for the Korean endemic species Chrysosplenium aureobracteatum (Saxifragaceae)

  • SHIN, Jae-Seo (Department of Life Science, Multidisciplinary Genome Institute, Hallym University) ;
  • KIM, Bo-Yun (National Institute of Biological Resources) ;
  • KIM, Yong-In (Multidisciplinary Genome Institute, Hallym University) ;
  • LEE, Jung-Hoon (Multidisciplinary Genome Institute, Hallym University) ;
  • KIM, Young-Dong (Department of Life Science, Multidisciplinary Genome Institute, Hallym University)
  • Received : 2020.01.17
  • Accepted : 2020.03.17
  • Published : 2020.03.31
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Abstract

Chrysosplenium aureobracteatum Y. I. Kim & Y. D. Kim (Saxifragaceae) is a recently described endemic species growing in the central part of the Korean peninsula. It requires constant monitoring for conservation due to its limited distributions. There is also a need for molecular markers for proper assessments of the genetic differentiation of C. aureobracteatum from species morphologically similar to it. In this study, we developed microsatellite markers that can be used to evaluate the genetic diversity of this species, representing fundamental data with which to conserve the natural populations of the species. A total of 17 expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed by the Illumina pair-end sequencing of the transcriptomes of C. aureobracteatum. These markers were successfully applied to populations of C. aureobracteatum and to its most closely related species, C. barbatum, revealing high polymorphism in both species. The EST-SSR markers developed in this study were proven to be useful not only to monitor the population genetic structure of C. aureobracteatum for conservation purposes but also to study the genetic delimitation of the species from species closely related to it.

Keywords

References

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