• Korean Journal of Environment and Ecology
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• v.33 no.5
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• pp.552-564
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• 2019

The purpose of this study was to investigate vegetation characteristics of evergreen broad-leaved forests in the area of Duryunsan Provincial Park, where the deciduous broad-leaved trees and evergreen broad-leaved trees are mixed and thus had a high botanical value. To investigate the vegetation characteristics, we installed 40 quadrats with an area of $100m^2$ each for survey and analysis. Haenam-gun, where the Duryunsan Provincial Park is located, is a warm-temperate forest region. The meteorological data for the past 40 years showed a coldness index of $-8^{\circ}C$, a monthly warmth index of $109.2^{\circ}C$, and annual mean precipitation of 1,310.5mm, indicating it is an ideal habitat for the distribution of evergreen broad-leaved forest. The results of community classification based on TWINSPAN showed three categories of vegetation communities in the surveyed region: Quercus acuta community-I, Q. acuta community-II, and Neolitsea sericea-Aphananthe aspera community. In the evergreen broad-leaved forest in the Duryunsan Provincial Park, Q. acuta dominant in the canopy were expanding their presence in the understory. In addition to the Q. acuta, N. sericea and Cinnamomum yabunikkei, which are evergreen broad-leaved trees of the canopy, were found in all layers. The deciduous broad-leaved trees such as Q. variabilis, Q. serrata, and Carpinus tschonoskii were culled, and the transition to Q. acuta evergreen broad-leaved trees was ongoing. N. sericea community appeared locally. The species diversity index of N. sericea-A. aspera community was lower at 1.0798 than that of Q. acuta Community-I at 1.3208 and Q. acuta Community-II at 1.4916.

• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.307-316
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• 2009

Creeping bentgrass (Agrostis palustrics Huds.) is cool season turfgrasse that is used for putting green in golf course. Creeping bentgrass cultivars are difficult to distinguish with the same species because of similar morphological characters and low level of genetic diversity. The SCAR markers using the specific DNA can be useful for differentiating between creeping bentgrass cultivars. Five RAPD primers were used for specific band detection among creeping bentgrass cultivars, penncross, penn A-4, crenshaw, L-93, CY-2, T-1. The pairs of SCAR primers for six cultivers were designed by the specific sequences of the bands that amplified by RAPD. Three of the six SCAR primers could not make the use as SCAR primers because the specific false bands were detected in all cultivars. The remaining pairs of SCAR primer, CY850F/R, T700F/R, L2900F/R, amplified the specific band at expected size for three cultivars, CY-2, T-1, L-93, respectively. The CY850F/R primer amplified a band of 850bp in CY-2 cultivar, the T700F/R primer amplified a band of 700bp in T-1 cultivar, and the L2900F/R primer amplified a band of 2.9kb in L-93 cultivar. In this study we developed the SCAR markers to identify and distinguish the inerseeded creeping bentgrass cultivars in a golf course green.

• Journal of Life Science
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• v.31 no.4
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• pp.377-384
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• 2021

Researching the soil fungal community is important to understand the interaction between fungi and living plants. However, too few studies have examined the soil fungal community and their interactions with plants. Rhododendron mucronulatum, commonly known as Korean rosebay, is an important forest resource that has aesthetic, ecological, and potential pharmacological values. We used a pyrosequencing method to analyze the characteristics of fungal communities from R. mucronulatum soil samples from Biseul mountain county park, which is one of the famous places for large R. mucronulatum colonies in South Korea. We collected soil core samples in February and August at three sites in the Biseul Mountain County Park, taking into consideration the regional and seasonal conditions. We obtained 454,157 validated reads after pyrosequencing all six samples. The fungal communities from the first observation spot in August had the richest species diversity among the samples. Basidiomycota, Ascomycota, and Mortierellomycota were major phyla in the samples. Agaricales_f, Mortierellaceae, and Clavariaceae were major families in the samples. The genus Mortierella was the most dominant in all six samples. Overall, 19 genera could be associated with R. mucronulatum. Sample 1 had 109 genera in sample 1, sample 2 had 111 genera, and sample 3 had 112 genera that were uniquely identified. The samples collected in August had 28 identified genera, that existed only in summer samples, indicating a weather effect. This study can be used as basic research to understand the relationship between soil fungi and plants.

• Korean Journal of Microbiology
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• v.55 no.1
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• pp.74-76
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• 2019

Gram-positive anaerobic bacilli Actinomyces spp. commonly reside on mucosal surfaces of the oropharynx, gastrointestinal tract, and urogenital tract. Here, we first report the draft genome sequence of Actinomyces georgiae KHUD_A1, isolated from dental plaque of a Korean elderly woman. The genome is 2,652,059 bp in length and has a GC content of 68.06%. The genome includes 2,242 protein-coding genes, 9 rRNAs, and 64 tRNA. We identified 157 KHUD_A1 strain-specific genes, including genes encoding CPBP family intramembrane metalloprotease, bile acid: sodium symporter family protein, Txe/YoeB family addiction module toxin and Phd/YefM family antitoxin. The sequence information of A. georgiae KHUD_A1 will help understand the general characteristics of the bacterial species and the genome diversity of the genus Actinomyces.

• Journal of agriculture & life science
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• v.52 no.6
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• pp.1-12
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• 2018

This study was conducted to analyze the openness of erosion control dams situated near mountain stream. The ecological functions of erosion control dams analyzed by such openness for adequately protect and manage the diversity of species and to prevent disasters. The obtained results were as follows. When structurally modifying or changing non-permeable, gravity type dams with a passing rate of less than 1% into open-type dams, the passing rate increased by about 77 times more from 0.72% to 55.8%. Except for closed, gravity type erosion control dams that are constructed with a special purpose such as creating sand deposits and reservoirs, there is a need to construct and improve the dams into permeable type dams that can relatively satisfactorily perform various functions such as carrying soil and sand to the downstream region and create a ecological corridor upstream and downstream for fish and amphibians. The openness based on the blocking height of the erosion control dam varies depending upon the height of the base part and the depth of the substructural part. It must be designed based on the on-site conditions and the purpose of the construction. The functional types of erosion control dams based on the open form of the cross-section as follows: the fish traffic type, flood control type, reservoir type for forest fire control), non-permeability type for soil and rock blocking, net type for blocking the rock flow and the particle screen type.

• Journal of Life Science
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• v.28 no.12
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• pp.1477-1488
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• 2018

This study investigated the muscles, intestines, and gonads of Sulculus diversicolor supertexta to examine the diversity of microbial communities within examples collected from the Jeju Coast. Using different media, initial pure isolation in MA, 1% BHIA, and 1% TSA indicated that the muscles, intestines, and gonads supported more communities, respectively. In analysis of relative similarity with 16s rRNA sequencing, 190 pure colonies were isolated, and further analysis with NBLAST identified 71 species, 39 genera, 25 families, and five phyla. Homogeny with the reference strain was 91-100%. Microbial communities in S. supertexta consisted of gamma and alpha Proteobacteria (48%), Actinobacteria (32.5%), Firmicutes (16.9%), Deinococcus-Thermus (1.3%), and Bacteroides (1.3%). In all tissue, Psychrobacter cibarius in Moraxellaceae was dominant. Alteromonadaceae, Enterobacteriaceae, Pasturellaceae, Moraxellaceae, Rhodobacteraceae, Geminicoccaceae, Dietziaceae, Intrasporangiaceae, Microbacteriaceae, Micrococcaceae, Micromonosporaceae, Streptomycetaceae, Aerococcaceae, Bacillaceae, Paenibacillaceae, Planococcaceae, and Staphylcoccaceae were commonly isolated across all tissues, and Flavobacteriaceae, Corynebacteriaceae, Yesiniaceae, Vibrionaceae, Hahellaceae, Pseudomonadaceae were also identified from the intestines. In microbial monitoring of four harmful bacteria, Streptomyces albus (96%) showed antibacterial activity against all four strains, and Agrococcus baldri (99%) and Psychrobacter nivimaris (99%) presented against E. Coli and E. aerogens. In addition, some strains with low homogeny were isolated and further experiments are therefore required, for example to refine the antimicrobial substances including new strain investigations. These additional experiments would aim to establish generic resources for the microbial communities in S. Supertexta and provide basic data for applied microbiological research.

• Journal of Korean Society of Forest Science
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• v.107 no.4
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• pp.378-384
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• 2018

Chinese jujube (Ziziphus jujuba Mill.), a member of family Rhamnaceae, is an economically important species. It has high nutritional value in fruits, and its medicinal properties have led to extensive use in traditional oriental medicine. In this study, we investigated the genetic characteristics of 270 jujube germplasms collected from the six major jujube fruit production areas in Korea including Boeun and Gyeongsan, in order to understand the current situation on cultivated varieties of domestic jujube and to obtain useful data for selection of superior germplasms and establishing their breeding plans. The results of ISSR marker analysis revealed that the Shannon's information index of the 270 germplasms was 0.107 and the genetic similarity was as high as 0.935 showing the dominance of a specific cultivar or germplasm, and almost 67% (180) of them were identified at the same genotype with 'Bokjo'. The domestic commercial jujube populations could be characterized by very low genetic diversity and thus may be vulnerable to external disturbances. In addition, it seems that most of the jujube fruits in domestic market were largely produced through parthenocarpy.

• Journal of Korean Society of Forest Science
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• v.108 no.1
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• pp.127-132
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• 2019

This study aimed to investigate the differences in habitat environment and characteristics of small rodent populations with time elapse after thinning in Japanese larch (Larix kaempferi) plantations, Mt. Janggun, Gyeongbuk Province, South Korea. We found that there were significant differences in habitat environment, number of tree, shrub stems, coverage of overstory, midstory, rock and number of coarse woody debris (CWD) between thinned and unthinned forest stands. We captured four small rodent species. Total number of captured small rodent were higher in thinned stands. Male-to-female ratio of Apodemus agrarius, and A. peninsulae were both the highest in forest stand 3 year after thinning. The differences in number of captured and sex ratio of small rodents might be related to habitat environment between thinned and unthinned stands. Thus, thinning can provide good habitat for small rodents by developing understory vegetation and increasing CWD in Japanese larch plantations. These results suggest that forest management through maintenance of CWD and induction of midstory and understory vegetation after thinning is necessary to maintain small rodents diversity.

• Journal of Life Science
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• v.31 no.1
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• pp.73-82
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• 2021

Throughout history, barley was the typical crop of the soils of Jeju Island due to its topographical features. People in Jeju eat Shindari or Dansul. Shindari or Dansul is a fermented drink of Jeju, made from the leftovers of cooked barely and nuruk of short fermentation periods. Although Makgeolli and Shindari share a similar fermentation period and materials, research on Shindari or Dansul is still in its early stages. In this study, we examined major bacterial species of Shindari or Dansul. In addition, we confirmed the antibacterial activities of an isolated strain against fish and human-harmful bacteria. Among the isolates, Firmicutes consisted of 73% and the Proteobacteria of 27%, indicating that the Firmicutes phylum was the dominant one. In addition, the Pediococcus genus and the Bacillus genus were the most prevalent consisting of 25%, followed by the Cronobacter genus (25%), the Enterococcus genus (16%), the Aneurinibacillus genus (5%), the Klebsiella genus (4%), and the Paenibacillus genus (2%). We conclude that the Lactobacillus genus predominated in Makgeolli, but the Pediococcus genus predominated in Shindari. In a study of the antibacterial activity, growth inhibition was observed for all bacteria, except for the fish disease bacterium Photobacterium damselae subsp. piscicida and the human-harmful bacterium Streptococcus mutans.

• Journal of the Korean Institute of Landscape Architecture
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• v.49 no.1
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• pp.42-53
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• 2021

Street trees improve the cityscape and air quality, reduce heat islands, and create wildlife habitats. Hence, they are essential parts of a city's green infrastructure. Therefore, several trees that are well adapted to the urban environment were planted. However, this caused the problem of simple trees being planted around the world. This study is to select more various street trees. To accomplish this, evaluation criteria and detailed indexes were created. The importance was indicated through the Analytic Hierarchy Process. For commercial roads, the priority of landscape characteristics is 0.2640, and among detailed indicators, the priority of shape is 0.1245. For work roads, the priority of landscape characteristics is 0.2496, and among detailed indicators, the priority of shape is 0.1177. For work roads, the priority of characteristics of civil service is 0.2250, and among detailed indicators, the priority of shape is 0.1177. For general roads, the priority of maintenance characteristics is 0.2479, and among detailed indicators, the priority of shape is 0.1062. For historical and cultural roads, the priority of regional characteristics is 0.3488, and among detailed indicators, the priority of regional characteristics is 0.1643. For ecological roads, the priority of ecosystem characteristics is 0.3488, and among detailed indicators, the priority of the diversity of species is 0.1643. For automotive-only roads, the priority of the ecosystem characteristics is 0.4639, and among detailed indicators, the priority of reducing emissions is 0.1643. This study will provide objective criteria for the selection of street trees.