• 제목/요약/키워드: soybean mosaic virus

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Seed Transmission Rates of Bean pod mottle virus and Soybean mosaic virus in Soybean May Be Affected by Mixed Infection or Expression of the Kunitz Trypsin Inhibitor

  • Nam, Moon;Bae, Hanhong;Hammond, John;Domier, Leslie L.;Youn, Young-Nam;Lee, Bong-Choon;Lim, Hyoun-Sub
    • Research in Plant Disease
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    • v.19 no.2
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    • pp.114-117
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    • 2013
  • To facilitate their spread, plant viruses have developed several methods for dispersal including insect and seed transmission. While insect transmission requires virus stability against insect digestion, seed-transmitted viruses have to overcome barriers to entry into embryos. Bean pod mottle virus (BPMV) is transmitted through seed at levels typically below 0.1%, but co-infection with Soybean mosaic virus (SMV) enhanced the seed transmission rate of BPMV in one experiment. In contrast, the rate of SMV seed transmission was not affected by BPMV co-infection. In a second preliminary study, the rate of SMV transmission was lower in an isoline of Williams 82 that contained a null mutation for the Kunitz trypsin inhibitor gene than in Williams 82. In this preliminary study, we observed that factors such as protease inhibitor expression and dual infection may affect the frequency of seed transmission of BPMV and SMV.

Identification of Alfalfa Mosaic Virus from Soybean (대두에서 발생한 알파파 모자이크 바이러스의 분류동정에 관한 연구)

  • Lee S. H.;Choi Y. M.;Kim J. S.;Chung B. J.
    • Korean Journal Plant Pathology
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    • v.1 no.1
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    • pp.33-37
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    • 1985
  • A yellow stripe and bud benting disease of soybean was commonly observed on the field at Suweon area. The causal agent was identified as alfalfa mosaic virus (AMV) by indicator plant reactions, physical properties, serological test and electron microscopy. AMV produced vein clearing, top necrosis, top bent and mottling on the parts of soybean plants. Local lesions were produced on the inoculated leaves of Vigna sesquipedialis, Vicia faba and Tetragonia expansa, while Chenopodium am, anticolor, C. quinoa, Pisum satvium, Petunia hybrida and Nicotiana tabacum 'Bright yellow' were systemically infected. The thermal inactivation point was $60^{\circ}C$, dilution end point was $10^{-3}$, and longevity in vitro was 2 days at room temperature. AMV from soybean was reacted with AMV - antiserum in agar gel diffusion test. Electron microscopy of AMV from soybean exhibited bacilliform particles of 60nm in length.

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Characterization of the in vitro Activities of the P1 and Helper Component Proteases of Soybean mosaic virus Strain G2 and Tobacco vein mottling virus

  • Lim, Hyoun-Sub;Jang, Chan-Yong;Nam, Ji-Ryun;Li, Meijia;Hong, Jin-Sung;Bae, Han-Hong;Ju, Ho-Jong;Kim, Hong-Gi;Ford, Richard E.;Domier, Leslie L.
    • The Plant Pathology Journal
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    • v.28 no.2
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    • pp.197-201
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    • 2012
  • Potyviruses express their RNA genomes through the production of polyproteins that are processed in host cells by three virus-encoded proteases. Soybean plants produce large amounts of protease inhibitors during seed development and in response to wounding that could affect the activities of these proteases. The in vitro activities of two of the proteases of Soybean mosaic virus (SMV) and Tobacco vein mottling virus (TVMV) were compared in the rabbit reticulocyte lysate in vitro translation system using synthetic RNA transcripts. Transcripts produced from SMV and TVMV cDNAs that included the P1 and helper component-protease (HC-Pro) coding regions directed synthesis of protein products that were only partially processed. Unprocessed poly-proteins were not detected from transcripts that included all of the P1, HC-Pro, P3 and portions of the cylindrical inclusion protein coding regions of either virus. Addition of soybean trypsin inhibitor to in vitro translation reactions increased the accumulation of the unprocessed polyprotein from TVMV transcripts, but did not alter the patterns of proteins produced from SMV. These experiments suggest that SMV-and TVMV-encoded proteases are differentially sensitive to protease inhibitors.

Interaction Study of Soybean mosaic virus Proteins with Soybean Proteins using the Yeast-Two Hybrid System

  • Seo, Jang-Kyun;Hwang, Sung-Hyun;Kang, Sung-Hwan;Choi, Hong-Soo;Lee, Su-Heon;Sohn, Seong-Han;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • v.23 no.4
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    • pp.281-286
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    • 2007
  • Interactions between viral proteins and host proteins are essential for virus replication. Especially, translation of viral genes completely depends on the host machinery. In potyviruses, interactions of genome-linked viral protein (VPg) with host translation factors including eIF4E, eIF(iso)4E, and poly(A)-binding protein (PABP) has previously been characterized. In this study, we investigated interactions between Soybean mosaic virus (SMV) viral proteins and host translation factors by yeast two-hybrid system. SMV VPg interacted with eIF4E, eIF(iso)4E, and PABP in yeast two-hybrid system, while SMV helper component proteinase (HC-pro) interacted with neither of those proteins. The interaction between SMV NIb and PABP was also detected. These results are consistent with those reported previously in other potyviruses. Interestingly, we found reproducible and specific interactions between SMV coat protein (CP) and PABP. Deletion analysis showed that the region of CP comprising amino acids 116 to 206 and the region of PABP comprising amino acids 520 to 580 are involved in CP/PABP interactions. Soybean library screening with SMV NIb by yeast two-hybrid assay also identified several soybean proteins including chlorophyll a/b binding preprotein, photo-system I-N subunit, ribulose 1,5-biphosphate carboxylase, ST-LSI protein, translation initiation factor 1, TIR-NBS type R protein, RNA binding protein, ubiquitin, and LRR protein kinase. Altogether, these results suggest that potyviral replicase may comprise a multi-protein complex with PABP, CP, and other host factors.

Effect of Planting Date on the Infection of Necrotic Soybean SMV (파종시기에 따른 콩 양저바이러스(SMV-IV)의 발생경향)

  • 신두철;김순권;황영현;박근용;김호일
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.24 no.3
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    • pp.59-65
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    • 1979
  • Severe outbreak of necrotic. soybean mosaic virus. (SMV -N) strain has been observed on soybean (Glycine max) in the central northern pans of Korea. Thirty soybean cultivars developed from Korea. Japan, and USA were tested for the estimation of infection percentages and ratings at 4 different planting dates. SMV-N infection was decreased by delaying the planting dates, but no differences were observed in conmon mosaic virus. Most of the soybean cultivars developed in Korea, and Japan were more susceptible to SMV- N than the US developed cultivars. Infection ratings of SMV-N showed highly significant negative correlations with common SMV regardless of planting dates.

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Affection of SMV -G5H Strain on Plant Growth and Seed Chemical Composition of Soybean Variety, Danyeobkong (콩 모자이크 바이러스 SMV-G5H 나병이 단엽콩 생육 및 종실 성분에 미치는 영향)

  • 김율호;노재환;김명기;임대준;이봉호;이상철
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.3
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    • pp.340-347
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    • 1996
  • Soybean [Glycjne max Merr. L.] variety 'Danyeobkong' was inoculated with soybean mosaic virus SMV-G5H strain in field and green house at Crop Experiment Station in 1995. The effects of this virus strain on plant characteristics and seed chemical composition of the soybean variety Danyeobkong were evaluated. Yield, the number of pod and 100 seed weight were significantly reduced by SMV-G5H strain infection. Inoculation of SMV-G5H strain of soybean mosaic virus caused the increment of seed protein content and the decrement of seed oil content in the soybean variety Danyeobkong. Changing pattern of fatty acid composition by SMV-G5H strain infection was varied according to the experimental place. In field trial, the percentage of linoleic and linolenic acids decreased and stearic and oleic acids increased in the seed oil from virus infected plants. In green house trial, however, linolenic and oleic acids were increased, whereas palmitic and linoleic acids were reduced.

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Growth Characteristics and Yield Potentials of Soybeans in Upland and Paddy Field (전과 답에서의 콩 생육특성과 수량성)

  • 황영현;박상구
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.4
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    • pp.336-342
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    • 1993
  • To obtain the basic information necessary for the development of soybean varieties well adaptable to upland-paddy field rotational croppings, the difference of growth characteristics between upland and paddy-field including yield potentials of current recommending soybean varieties were evaluated. The growth characteristics, both above and under-ground, which were measured at flowering stage were generally greater in paddy-field but the number of root nodules was much greater in upland, thus the artificial inoculation was practically recommended for soybean growing in paddy-fields. Mean seed yield was generally higher in paddy-fields than in upland. All soybean varieties showed higher seed yield in the early planting date, April 20, were somewhat susceptible to soybean mosaic virus(SMV), thus they could be escaped from the disasterous endemic necrotic soybean mosaic virus(SMV-N). Soybean varieties showed over 4.0 tons/ha seed yield in the paddy-field were Williams 79, Union, SS77053, and Namhaekong. At the same time, Jangyeobkong and Danyeobkong were the most stable soybean varieties among the tested soybean varieties with less than 10% of coefficient variation values in all planting dates in paddy fields. Compared with Hwangkeumkong which is most widely being cultivated on farmer's fields, soybean varieties showed high yields in paddy-field were higher in plant height, less in the number of branches, and more in the number of nodes on main stem. At the same time, they had medium seed size which would bring the good germination and stands. Disease resistance especially for necrotic soybean mosaic virus was also one of the most decisive factors in seed yields for the early planted soybeans.

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Complete nucleotide sequences of an Rsv-resistance overcoming isolate of soybean mosaic virus.

  • Park, Bong-Kum;Ahn, Hye-Jin;Yum, Hye-Jung;Lee, Jae-Hwa;Park, Chang-Won;Ryu, Ki-Hyun
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.76.2-77
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    • 2003
  • The complete nucleotide sequences of genomic RNA of an isolate of soybean mosaic virus (SMV-CN18), which has ability to overcome Rsv resistance of soybean, have been determined. A large open reading frame encodes a polyprotein of 3068 amino acids with a predicted Mr of 350 kDa. Based on comparison with the proposed cleavage site of other potyviral polyproteins, nine mature proteins are predicted as a following order, P1, HC-Pro, P3, CI, 6K, VPg, NIa, NIb and coat protein (CP). The mature proteins of the strain share various amino acid identity with known SMV-G2, -G7 and -N strain, with the greatest variability occurring in the P1 (91 %, 88 %, 96%)and the lowest variability in the CP (100 %, 99 %, 100 %). In addition, 5' untranslated region determined by 5' RACE is much more various than any coding regions. Difference in amino acid sequences throughout the genome is discussed in relation to resistance and susceptibility of soybean cultivars to SMV-CNl8.

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