Korean Journal Plant Pathology (한국식물병리학회지)

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification of Alfalfa Mosaic Virus from Soybean

대두에서 발생한 알파파 모자이크 바이러스의 분류동정에 관한 연구

  • Lee S. H. (Institute of Agricultural Sciences, Department of Plant Pathology, Office of Rural Development) ;
  • Choi Y. M. (Institute of Agricultural Sciences, Department of Plant Pathology, Office of Rural Development) ;
  • Kim J. S. (Institute of Agricultural Sciences, Department of Plant Pathology, Office of Rural Development) ;
  • Chung B. J. (Institute of Agricultural Sciences, Department of Plant Pathology, Office of Rural Development)
  • 이순형 (농촌진흥청 농업기술연구소 병리과) ;
  • 최용문 (농촌진흥청 농업기술연구소 병리과) ;
  • 김정수 (농촌진흥청 농업기술연구소 병리과) ;
  • 정봉조 (농촌진흥청 농업기술연구소 병리과)
  • Published : 1985.01.01
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Abstract

A yellow stripe and bud benting disease of soybean was commonly observed on the field at Suweon area. The causal agent was identified as alfalfa mosaic virus (AMV) by indicator plant reactions, physical properties, serological test and electron microscopy. AMV produced vein clearing, top necrosis, top bent and mottling on the parts of soybean plants. Local lesions were produced on the inoculated leaves of Vigna sesquipedialis, Vicia faba and Tetragonia expansa, while Chenopodium am, anticolor, C. quinoa, Pisum satvium, Petunia hybrida and Nicotiana tabacum 'Bright yellow' were systemically infected. The thermal inactivation point was $60^{\circ}C$, dilution end point was $10^{-3}$, and longevity in vitro was 2 days at room temperature. AMV from soybean was reacted with AMV - antiserum in agar gel diffusion test. Electron microscopy of AMV from soybean exhibited bacilliform particles of 60nm in length.

황록색줄무늬모자익과 줄기 정단부가 구부러지는 증상을 보이는 대두 이병주를 채집하여 지표식물검정, 충매전염, 물리적 성질 조사, 항혈청검정 및 전자현미경검경을 하였다. 지표식물검정결과 T. expansa와 Scotia에 국부병반반응, 동부, 완두, 명아주 등에 전신감염 증상을 일으켰다. 복숭아혹진딧물에 의한 전염율은 $90\%$였다. 물리적 성질은 내열성이 $60^{\circ}C$, 내희석성이 1,000배였고, 내보존성이 2일이었다. 바이러스를 순화하여 항혈청제조결과 1:1024의 역가를 보였으며 일본 바이러스연구소에서 분양 받고 AMV 항혈청과 양성반응을 보였다. 전자현미경검경 결과 대부분 길이가 60nm인 박테리아 모양의 바이러스 입자가 검경되었다.

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