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http://dx.doi.org/10.5423/PPJ.2012.28.2.197

Characterization of the in vitro Activities of the P1 and Helper Component Proteases of Soybean mosaic virus Strain G2 and Tobacco vein mottling virus  

Lim, Hyoun-Sub (Department of Applied Biology, Chungnam National University)
Jang, Chan-Yong (Department of Applied Biology, Chungnam National University)
Nam, Ji-Ryun (Department of Applied Biology, Chungnam National University)
Li, Meijia (Department of Applied Biology, Chungnam National University)
Hong, Jin-Sung (Institute of Natural Sciences, Seoul Woman's University)
Bae, Han-Hong (School of Biotechnology, Yeungnam University)
Ju, Ho-Jong (Department of Agricultural Biology, Chonbuk National University)
Kim, Hong-Gi (Department of Applied Biology, Chungnam National University)
Ford, Richard E. (Department of Crop Sciences, University of Illinois)
Domier, Leslie L. (Department of Crop Sciences, University of Illinois)
Publication Information
The Plant Pathology Journal / v.28, no.2, 2012 , pp. 197-201 More about this Journal
Abstract
Potyviruses express their RNA genomes through the production of polyproteins that are processed in host cells by three virus-encoded proteases. Soybean plants produce large amounts of protease inhibitors during seed development and in response to wounding that could affect the activities of these proteases. The in vitro activities of two of the proteases of Soybean mosaic virus (SMV) and Tobacco vein mottling virus (TVMV) were compared in the rabbit reticulocyte lysate in vitro translation system using synthetic RNA transcripts. Transcripts produced from SMV and TVMV cDNAs that included the P1 and helper component-protease (HC-Pro) coding regions directed synthesis of protein products that were only partially processed. Unprocessed poly-proteins were not detected from transcripts that included all of the P1, HC-Pro, P3 and portions of the cylindrical inclusion protein coding regions of either virus. Addition of soybean trypsin inhibitor to in vitro translation reactions increased the accumulation of the unprocessed polyprotein from TVMV transcripts, but did not alter the patterns of proteins produced from SMV. These experiments suggest that SMV-and TVMV-encoded proteases are differentially sensitive to protease inhibitors.
Keywords
Helper-component protease; in vitro translation; P1 protease; trypsin inhibitor;
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