• 한국식품과학회지
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• 제36권5호
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• pp.723-727
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• 2004

콩 가공식품인 두유, 두부, 비지에서 유전자변형 콩에 삽입된 epsps 유전자의 검출과 정량을 위해 정성과 정량 PCR 방법을 수행하였다. 두유, 두부, 비지에서 유전자변형 콩의 검출은 삽입된 epsps 유전자의 증폭이 크기가 121bp와 330bp가 생성될 수 있는 두 종류의 primer들을 이용하여 0.01%까지 확인하였다. 정량방법은 1, 3, 5%의 유전자변형 콩이 포함된 시료들을 실시간 PCR을 사용하여 유의성 있는 결과를 얻었다. 이러한 결과들은 실시간 PCR 방법을 사용하여 가공식품 내에서 정량적으로 유전자변형 콩을 정량하는데 적용될 수 있음을 보였다.

• 한국농촌생활과학회지
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• 제9권1호
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• pp.33-42
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• 1998

The purpose of this study was to find out the dietary attitudes and behaviors, intake frequencies of food groups, health-related opinions and practices, and sociodemographic characteristics of housewives in Wonju-Gun rural area. Interviewers asked 413 housewives about obove informations and then recorded. The results were as followes : Using the factor analysis processing, 17 questions about dietary attitudes and behaviors were categorized into 5 groups such as fidelity of diet, the healthful dietary attitudes, the eating practices concerning about items and amount, eating speed and the extent of coincidence in food preference within a family. The role of the housewives was a major factor affecting the dietary attitudes and behaviors. Those who work as housewives and farmers concurrently had good dietary practices concerning about items and amount, but they were interested about health-directed dietary attitudes at a significantly lower level. Food frequencies of 9 groups, animal protein food, fruits and vegetables, milk and milk products, small fishes such as anchovy, soybean curd and beans, seaweeds, vegetable oil, frying food, soybean paste were significantly associated with subjects' age, role, and education and income level. Whether or not intake of soybean paste was considered. protein food intake was altered, and the difference between two situations increased much more as age was higher. We noticed that intake of soybean paste improved the status of protein nutrition. Our subjects ate calcium sources and dishes using vegetable oil insufficiently. Those who had much interests for good health, revealed to be an active practitioner in the aspects of healthful dietary attitudes, but fidelity of diet, eating practices concerning about items and amount showed significant association with balanced life style.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.23-23
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• 2022

Recently, soybean production and market price are unstable, even if demand of soybean is maintained. Diverse conditions such as climate change, a decrease in rural population, and consuming affect food industry. In this situation, food security is soaring as important key-word again, and MAFRA is promoting policies for improving soybean self-sufficiency with the goal of 40% until 2030. The point of policy is to extend a production and stabilize a demand for soybean with supporting large-scale soybean paddy-field complex. According to the background, soybean breeding and production research in NICS are proceeded with three parts. First, production improvement with soybean cultivation land enlargement and high-yield cultivar development. Various growth period soybean cultivars for double cropping, irrigation management technologies in paddy field, and hyper-yield and specific-region adaptable cultivar development. Second, reduction of production expense with mechanized cultivation and digital-based field management technologies. Third, consumer-friendly and high quality soybeans with high protein cultivar for alternative protein usage and high food process-ability for soy milk, tofu, soybean sprouts, and grain usage. Each part need to be combined and advanced to improve soybean industry and soybean self-sufficiency.

• 한국식품조리과학회지
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• 제9권4호
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• pp.278-283
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• 1993

This study was attempted to investigate the effects of enzymatic modification of milk protein with protease on functional properties. The selected functional properties were solubility, emulsifying activity (EA), emulsion stability(ES), foam expansion(FE), and foam stability(FS). These properties were measu-red from pH 3.0 to pH 8.0. The proteases used in this study were iaolated from Meju(fermemted soybean) and had specific activity of 250 units/㎎ protein at pH 7.0, 1600 units of pretense was used for 1gr. of skim milk protein. Skim milk showed 30.5％ degree of hydrolysis for 1 hr. and 36.4% degree of hydrolysis for 3.5 hrs. of protease treatment at pH 7.0. Solubility of native skim milk, control, 1 hr. and 3.5 hrs. groups were 3.37, 3.64, 10.21, 14.34％o at pH 4.0 respcetively. The emulsifying activity of native skim milk, control, 1 hr. and 3.5 hrs. groups were 38.8,42.0,43.0,46.7ft at pH 4.0, respectively. Enzymatic modification resulted in the increase of solubility and emulsifying activity at pH 4.0. However at pH 5.0 emulsifying activity of 1 hr. and 3.5 hr. group were lower than native skim milk and control groups. 1 hr. protease treatment was found to be most effective way of increasing foam expansion at pH 4.0 to 6.0. It was supported that, protease treated skim milk can be used to improve solubility, emulsifying activity, foam expansion at acid pH. meju protease. skim milk, solubility, emulsion, foam.

• 한국식품과학회지
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• 제40권1호
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• pp.88-95
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• 2008

본 연구는 콩 아이스크림의 품질향상을 위하여 콩을 데치고 동결 건조 한 후 분말화하여 과즙을 첨가하고 혼합 유산균 종균을 배양시켜 대두분말 발효 베이스를 제조한 후 올리고당을 첨가하여 식감개선, 저칼로리 및 혈당 수준의 개선 특성을 가진 콩 아이스크림을 제조하여 그 품질 특성과 혈당개선능을 비교하였다. 대두분말을 $121^{\circ}C$에서 15분간 가압멸균한 후 $30^{\circ}C$로 냉각한 다음, L. acidophillus KTCT 3164와 L. bulgaricus KCTC 3635 현탁액을 $4.6}{\times}106$ CFU/mL의 농도를 1:1 비율로 혼합한 균주(1%)를 접종하고 $33^{\circ}C$에서 24시간 발효시킨 후 비교한 결과, 삶은 대두분말 발효베이스가 볶은 대두분말 발효 베이스에 비해 pH는높았고 점도는 낮았다. 생균수는 접종 후 24시간 배양 후 삶은 대두분말 발효 베이스의 유산균 생육이 우세하였다. 관능검사법으로 평가한 대두분말 발효베이스의 종합적인 기호도는 삶은 대두분말 발효베이스가 콩 특유의 비린 맛과 냄새가 약하면서 상큼한 풍미가 많고 입안의 질감이 부드러워서 관능적인 품질이 양호한 것으로 나타났다.볶은 대두분말 베이스 아이스크림이 overrun과 녹아내리는 정도가 가장 낮았고, 이에 비해 삶은 대두분말 발효베이스 아이스크림은 overrun과 녹아내리는 정도가 증가하여 품질 특성이 현저하게 향상되었다. 냉동에 의한 생균수의 변화는 삶은 대두분말 발효베이스 아이스크림군이 볶은 대두분말 발효베이스 아이스크림군보다 높게 나타났으나, 같은 아이스크림 군 간에는 냉동에의한 생균수의 유의적인 차이는 없었다. 관능검사에 의한 아이스크림의 텍스쳐는 우유아이스크림이 가장 높게 평가되었으나 삶은 대두분말 발효베이스 아이스크림과 유의한 차이는 없었다. 종합적인 기호도는, 우유 아이스크림과 유의한 차이는 보이지 않았음에도 불구하고, 삶은 대두분말 발효베이스 아이스크림이 가장 양호하게 평가되었다. 건강한 20대 여성들을 대상으로 아이스크림의 경구 혈당반응 검사 결과, 설탕 첨가 우유 아이스크림과 올리고당 첨가 삶은 대두분말 발효베이스 아이스크림 사이에 혈당 면적은 유의한 차이가 없었으나, 아이스크림 섭취 후 120분 혈당은 올리고당 첨가 삶은 대두분말 발효베이스 아이스크림을 먹은 경우가 설탕 첨가 우유 아이스크림을 먹은 경우에 비해 유의하게 낮았다. 또한 혈당 증가가 완만하여 식사 후 혈당관리가 중요한 당뇨환자에게는 일반 아이스크림보다 유익할 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권4호
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• pp.502-511
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• 2005

The effects of different levels of crude protein (CP) and cottonseed meal replacing for soybean meal in cassava chips and rice straw-based diets for mid-lactating cows (100-150 day in milk [DIM]), were studied using 32 multiparous Holstein Fresian crossbred dairy cows. Diets containing 10.5, 12.5, 13.7, 14.4% CP of the rations and 0, 12.1, 14.9, 17.8% cottonseed meal were fed to cows for 60 days. Thirty two cows were randomly divided into four dietary treatments using a Randomized complete block design. Four dietary treatments were offered in the form of total mixed ration (TMR) with concentrate to roughage (chopped rice straw) at 60:40 and offered ad libitum. Dry matter (DM) and neutral detergent fiber (NDF) intakes tended to linearly increase with increasing dietary CP levels. Intakes and digestibility of crude protein increased linearly with increasing dietary CP level (p<0.01). Crude protein digestibility of the 10.5% CP diet was lower (p<0.05) than that in diets with higher levels of CP, while there were no significant differences among the other three levels of CP (12.5, 13.7 and 14.4%). Daily milk yield tended to increase with increased CP from 10.5 to 14.4%. Income over feed in terms of US$/kg of milk increased with increased CP from 10.5 to 13.7% and decreased when the CP level was higher than 13.7% (quadratic effect p<0.09). Milk composition was not significantly affected by increasing level of CP, however there were relatively high contents of protein and fat among treatments. The proportion of milk-urea N (MUN), ammonia-N ($NH_3$-N) and bloodurea N (BUN) were closely correlated and increased linearly with increasing CP levels (p<0.01). Balanced diet was found in diet containing 12.5 and 13.7% CP of the rations when BUN and MUN were used as indicators of the protein to energy ratio in the diet. Conclusions can be made that increasing dietary CP levels from 10.5 to 13.7% using cottonseed meal as the main source to completely replace soybean meal was beneficial to cows consuming rice straw and cassava chips based-diets. Increasing the CP level above 13.7% of total ration did not additionally improve milk yield and composition or net income.

• 한국유기농업학회지
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• 제18권4호
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• pp.599-612
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• 2010

본 연구는 유기곡물사료와 농산가공부산물인 미강과 대두피를 이용한 유기배합사료를 제조하였으며, 관행사료와 비교하여 산양유의 생산성 및 경제성에 미치는 영향을 조사하기 위함이다. Saanen종 비유중기 4두를 유량, 착유일수 및 산차수를 고려하여 배치하였으며, 시험 설계는 2${\times}$3 crossover design으로 하였으며, 2주간 예비기간을 거쳐 본 시험을 실시하였다. 사료공급은 에너지 및 단백질 공급량에서 ME가 23 MJ/d, 그리고 CP는 382 g/d가 되도록 조정하였다. 전체 건물섭취량은 T1구(관행사료)와 T2구(유기사료)에서 각각 1,633g과 1,658g으로 비슷한 섭취량을 나타냈으며, 유량에서는 유의한 차이는 없었지만, T2구에서 1,816g/d로 T1구의 1,667g/d와 비교하여 약 150g/d 높은 유량 증가를 나타냈다. 유성분과 유지방 함량에서는 처리간 차이를 나타내지 않았으나, 지방산조성은 사료내 불포화지방산 함량이 풍부한 T2구에서 T1구보다 stearic acid(C18:0)와 linoleic acid(C18:2)가 유의하게 높았다. 또한 경제성 평가에서 1마리당 하루 150원의 소득증가를 나타냈으며 결과적으로 유기사료를 급여함으로써 농가소득에 기여할 것으로 판단된다.

• 한국식품영양학회지
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• 제13권2호
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• pp.118-124
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• 2000

Lipoxygenase(LOX) in soybeans is responsible for beany flavors which limit the wide utilization of soybeans to foods. This study was conducted to analyze beany flavor compounds of the normal Hwagkeumkong and LOX-deficient soybean cultivars, Jinpumkong which lacks L-2, L-3, and Jinpumkong 2 which lacks all L-1, L-2, L-3. Using the combination of dynamic headspace sampling and gas chromatography-mass selective detector(DHS-GC-MSD) for analyzing volatile compounds, hexanal and hexanol were identified in whole soy flour of all three soybena cultivars. Hwangkeumkong had more volatile compounds than Jinpumkong and Jinpumkong 2 in defatted soy flour. Hexanal and acetic acid were identified in soy milk of all three soybean cultivars but Hwangkeumkong had more volatile compounds than Jinpumkong 2. From the analysis with a static headspace sampling(SHS) and GC-MSD the major compounds were hexanal, acetic acid, 1-hexanol, and 1-octen-3-ol. The content of acetic acid was similar among three cultivars. But contents of hexanal and pentanal in Jinpumkong 2 were less than that of Jinpumkong and Hwangkeumkong. Using GC-FID, Jinpumkong 2 had less contents of hexanal and pentanol than Hwangkeumkong in whole soy flour and defatted soy flour. In this study, LOX-deficient soybean cultivars showed less hexanal, pentanol and other compounds than the normal Hwangkeumkong. However quite amount of beany flavor compounds were identified in Jinpumkong and Jinpumkong 2. So further studies are required to characterize LOX isozymes, to understand the mechanisms of beany flavors production, and to develop some other methods for removing beany flavor.

• Asian-Australasian Journal of Animal Sciences
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• 제13권3호
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• pp.329-333
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• 2000

Data of 15 multiparous Holstein cows kept at Ibaraki Prefectural Animal Experiment Station were collected from 10 weeks prepartum to 20 weeks postpartum. Cows were assigned randomly to a soybean meal (S8M) diet or a fish meal (FM) diet from 4 weeks before expected calving date to 20 weeks postpartum. Each diet was formulated to contain similar amounts of CP, ADF, and NDF. In the FM diet, 2.5 and 5% of fish meal were supplemented as total mixed rations in prepartum and postpartum periods, respectively. Compared to the SBM diet, undegraded intake protein (UIP) and Met were higher in the FM diet, but Lys was low. Body weight and dry matter intake were not affected by supplemental FM, and dry matter intake increased by 6 weeks postpartum and maintained constant after 7 weeks postpartum. Cows in the FM diet remained high milk production during the experimental period, but milk yield in the SBM diet decreased gradually after 6 weeks postpartum. Supplemental FM increased milk yield and protein yield from 10 to 20 weeks postpartum when FM intake was 1.19 kg/d, although milk protein was not improved. There were no significance differences in fat content and fat yield between FM and SBM diets. Supplemental FM had no effect on plasma glucose and urea-N at parturition and 7 weeks postpartum. Thus, the increased milk and protein yield may be due to the combination of carryover effect of supplemental UIP or Met in FM from 4 weeks prepartum to 10 weeks postpartum and direct effect of supplemental FM.

• Asian-Australasian Journal of Animal Sciences
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• 제33권11호
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• pp.1779-1786
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• 2020

Objective: To evaluate soy sauce oil (a by-product of making whole soybean soy sauce) as a new dietary lipid source, a large amount of soy sauce oil was administered into the rumen of dairy cows. Methods: Four Holstein dairy cows fitted with rumen cannulae were used in a 56-day experiment. Ruminal administration of soy sauce oil (1 kg/d) was carried out for 42 days from day 8 to day 49 to monitor nutritional, physiological and production responses. Results: Dry matter intake and milk yield were not affected by soy sauce oil administration, whereas 4% fat-corrected milk yield and the percentage of milk fat decreased. Although ruminal concentration of total volatile fatty acids (VFA) and the proportion of individual VFA were partially affected by administration of soy sauce oil, values were within normal ranges, showing no apparent inhibition in rumen fermentation. Administration of soy sauce oil decreased the proportions of milk fatty acids with a carbon chain length of less than 18, and increased the proportions of stearic, oleic, vaccenic and conjugated linoleic acids. Conjugated linoleic acid content in milk became 5.9 to 8.8 times higher with soy sauce oil administration. Blood serum concentrations of non-esterified fatty acid, 3-hydroxybutyric acid, total cholesterol, free cholesterol, esterified cholesterol, triglyceride and phospholipid increased with administration of soy sauce oil, suggesting a higher energy status of the experimental cows. Conclusion: The results suggest that soy sauce oil could be a useful supplement to potentially improve milk functionality without adverse effects on ruminal fermentation and animal health. More detailed analysis is necessary to optimize the supplementation level of this new lipid source in feeding trials.