• Title/Summary/Keyword: sources of resistance

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Evaluation of Septoria Brown Spot Disease and the Disease Resistance in Soybean Cultivars (대두(大豆)갈색무늬병의 병진전(病進展)과 품종간저항성(品種間抵抗性) 검정(檢定))

  • Oh, Jeung-Haing
    • Korean journal of applied entomology
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    • v.24 no.2 s.63
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    • pp.103-106
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    • 1985
  • The present study was carried out to determine appropriate growth stage for evaluating resistance to septoria brown spot in field and to search resistance sources from soybean germ plasm. Disease severity expressed by log $\frac{x}{1-x}$ was different with soybean genotypes and vertical progress of the disease was related to the diseased leaf area. Correlation between diseased leaf area and the area under septoria brown spot disease progress curve (AUBC) was highest at full blooming stage, indicating a reasonable stage for measuring the disease severity to evaluate resistance in field. There was no lines highly resistant to the disease among 1,428 native soybean lines tested.

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Simplex PCR Assay for Detection of blaTEM and gyrA Genes, Antimicrobial Susceptibility Pattern and Plasmid Profile of Salmonella spp. Isolated from Stool and Raw Meat Samples in Niger State, Nigeria

  • Musa, Dickson A.;Aremu, Kolawole H.;Ajayi, Abraham;Smith, Stella I.
    • Microbiology and Biotechnology Letters
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    • v.48 no.2
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    • pp.230-235
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    • 2020
  • The global evolution of antibiotic resistance has threatened the efficacy of available treatment options with ravaging impacts observed in developing countries. As a result, investigations into the prevalence of antibiotic resistance and the role of plasmids are crucial. In this study, we investigated the presence and distribution of blaTEM and gyrA genes, plasmid profiles, and the antimicrobial susceptibility pattern of Salmonella strains isolated from raw meat and stool sources across Niger State, Nigeria. Ninety-eight samples, comprising 72 raw meat and 26 stool samples, were screened for Salmonella spp. The antimicrobial susceptibility of Salmonella isolates to 10 commonly used antimicrobial agents was determined using the KirbyBauer disc diffusion method. Isolates were further analyzed for plasmids, in addition to PCR amplification of beta-lactamase (blaTEM) and gyrA genes. A total of 31 Salmonella spp. were isolated, with 22 from raw meat (70.97%) and 9 from stool (29.03%). Salmonella spp. with multiple resistance patterns to ceftazidime, cefuroxime, ceftriaxone, erythromycin, ampicillin, cloxacillin, and gentamicin were detected. Ofloxacin and ciprofloxacin were found to be the most effective among the antibiotics tested, with 67.7% and 93.5% susceptible isolates, respectively. Nine (29.03%) isolates harbored plasmids with molecular sizes ranging between 6557 bp and 23137 bp. PCR amplification of gyrA was detected in 1 (3.23%) of the 31 isolates while 28 isolates (90.32%) were positive for blaTEM. This study shows the incidence of antibiotic resistance in Salmonella isolates and the possible role of plasmids; it also highlights the prevalence of ampicillin resistance in this local population.

Food-borne outbreaks, distributions, virulence, and antibiotic resistance profiles of Vibrio parahaemolyticus in Korea from 2003 to 2016: a review

  • Park, Kunbawui;Mok, Jong Soo;Kwon, Ji Young;Ryu, A Ra;Kim, Song Hee;Lee, Hee Jung
    • Fisheries and Aquatic Sciences
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    • v.21 no.2
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    • pp.3.1-3.10
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    • 2018
  • Background: Vibrio parahaemolyticus is one of the most common causes of seafood-borne illnesses in Korea, either directly or indirectly, by consuming infected seafood. Many studies have demonstrated the antibiotic susceptibility profile of V. parahaemolyticus. This strain has developed multiple antibiotic resistance, which has raised serious public health and economic concerns. This article reviews the food-borne outbreaks, distributions, virulence, and antibiotic resistance profiles of V. parahaemolyticus in Korea during 2003-2016. Main body: V. parahaemolyticus infections appeared to be seasonally dependent, because 69.7% of patient infections occurred in both August and September during 2003-2016. In addition, the occurrence of V. parahaemolyticus in marine environments varies seasonally but is particularly high in July, August, and September. V. parahaemolyticus isolated from aquaculture sources on the Korean coast varied in association with virulence genes, some did not possess either the tdh (thermostable direct hemolysin) or trh (tdh-related hemolysin) genes, and a few were positive for only the trh gene or both genes. The high percentage of ampicillin resistance against V. parahaemolyticus in the aquatic environment suggests that ampicillin cannot be used to effectively treat infections caused by this organism. Short conclusion: This study shows that the observed high percentage of multiple antibiotic resistance to V. parahaemolyticus is due to conventionally used antibiotics. Therefore, monitoring the antimicrobial resistance patterns at a national level and other solutions are needed to control aquaculture infections, ensure seafood safety, and avoid threats to public health caused by massive misuse of antibiotics.

Serotypes and Sensitivity Distribution of Pseudomonas aeruginosa (임상재료에서 분리된 녹농균의 혈청형과 약제 감수성과의 관계)

  • Park, Kwang-Woong;Cho, Yang-Ja
    • The Journal of the Korean Society for Microbiology
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    • v.17 no.1
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    • pp.57-66
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    • 1982
  • The correlation between the serotypes and sensitivity distribution of Pseudomonas aeruginosa was studied with stock strains of department, strains isolated from various clinical sources and strains of haspital environment of the past two years. Two hundred and fifty seven strains were typable and 38 strains were untypable out of 295 clinical sorces. Serotype B was most provalent(27.6%) followed by serotype G(24.9%), serotype E(23.3%) and serotype A(7.8%) among 257 typable strains. However, no serotype J,L and M were observed. Serotype B,G,E and A were isolated from pus, sputum, wound, burn site and urine. Serotypes E were most frequently isolated from nasal discharge and serotypes K were isolated from pus. There were no apparent differences in sensitivity distribution of streptomycin, carbenicillin and ampicillin between the clinical and The environmental origin. However, the strains of environmental origin were found to be relatively more susceptable to tetracycline, gentamicin and fradiomycin than the strains of clinical sources in high concentration. The strains of clinical sources of serotype B,G,E and A showed different resistant patterns to all antimicrobial agents except for carbenicillin. Serotype E showed the highest percentage resistance followed by serotype G,B and A.

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Inoculum Sources to Generate High Mechanical Transmission of Barley yellow mosaic virus

  • Jonson, Gilda;Kim, Yang-Kil;Kim, Mi-Jung;Park, Jong-Chul;Hyun, Jong-Nae;Kim, Jung-Gon
    • The Plant Pathology Journal
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    • v.23 no.2
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    • pp.103-105
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    • 2007
  • Mechanical transmission of barley seedlings with barley yellow mosaic virus (BaYMV) is generally inefficient and is the major constraint for testing cultivar resistance to the virus. To explore mechanical transmission, BaYMV-infected barley plants were grown at different conditions and used as inoculum sources to seedlings of susceptible barley cultivar Baegdong. Extracts prepared from BaYMV-infected Baegdong plants at 47, 53, 74, and 90 days after symptom appearance (DASA) and grown at 10 and $12^{\circ}C$ gave 10, 30, 68 and 76% infection, respectively on inoculated susceptible barley cv. Baegdong seedlings. While Jinyangbori, another susceptible cultivar obtained 95% infection rate inoculated with extracts from 90 DASA disease source and grown at $10/12^{\circ}C$. However, low infection rates were obtained when the virus sources were grown in a greenhouse at $15-18^{\circ}C$. Our results indicate that longer incubation period and lower temperature are required for virus accumulation and stability.

Design and Verification of Improved Cascaded Multilevel Inverter Topology with Asymmetric DC Sources

  • Tarmizi, Tarmizi;Taib, Soib;Desa, M.K. Mat
    • Journal of Power Electronics
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    • v.19 no.5
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    • pp.1074-1086
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    • 2019
  • This paper presents the design and implementation of an improved cascaded multilevel inverter topology with asymmetric DC sources. This experimental inverter topology is a stand-alone system with simulations and experiments performed using resistance loads. The topology uses four asymmetric binary DC sources that are independent from each other and one H-bridge. The topology was simulated using PSIM software before an actual prototype circuit was tested. The proposed topology was shown to be very efficient. It was able to generate a smooth output waveform up to 31 levels with only eight switches. The obtained simulation and experimental results are almost identical. In a 1,200W ($48.3{\Omega}$) resistive load application, the THDv and efficiency of the topology were found to be 1.7% and 97%, respectively. In inductive load applications, the THDv values were 1.1% and 1.3% for an inductive load ($R=54{\Omega}$ dan L=146mH) and a 36W fluorescent lamp load with a capacitor connected at the dc bus.

Potato breeding via protoplast fusion (원형질체 융합을 이용한 감자 육종)

  • Cho, Kwang-Soo;Park, Tae-Ho
    • Journal of Plant Biotechnology
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    • v.41 no.2
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    • pp.65-72
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    • 2014
  • Plant cells from which the cell walls have been enzymatically or mechanically removed are called protoplasts. The protoplasts are theoretically totipotent and can be used as sources of somatic cell fusion in practical breeding programs. Wild Solanum species have often been used as sources of important agricultural traits including diverse disease resistance. However, they cannot often be directly applied to breeding programs due to their sexual incompatibility with S. tuberosum. Somatic hybridization via protoplast fusion is one of the ideal methods to overcome this limitation and to introgress certain traits into S. tuberosum. This technique has still widely been used in potato since the first fusion was reported in 1970s. Therefore, this review highlights general perspectives of protoplast fusion and discusses the application of protoplast fusion in potato breeding.

Bondability of Different Electronic Materials by Micro Heat source (마이크로 열원에 의한 이종전자재료의 접합성)

  • 이철인;서용진;신영의;장의구
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1994.11a
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    • pp.206-209
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    • 1994
  • This paper has been researched bondability of electronic devices, such as lead frame and thick film of Ag/Pd on an alumina substrate by different heat sources. To obtain the bonds with high quality, it is very important to control both the thermal distribution of the bonds and it stability, because electronics components is consist of different materials. Therefore, this paper clarifies not only heat mechanism of micro parallel gap resistance bonding method and pulse heat tip bonding method but also investigates selection of heat sources with micro-electronic materials for bonding. Finally, it is realzed fluxless bonding process with filler metal such as plating layers.

Equivalent Noise Charge Measurements in Hydrogenated Amorphous Silicon Radiation Detectors

  • Kim, Ho-Kyung;Hur, Woo-Sung;Gyuseong Cho
    • Proceedings of the Korean Nuclear Society Conference
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    • 1995.05a
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    • pp.973-979
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    • 1995
  • The input equivalent noise charge (ENC) of hydrogenated amorphous silicon radiation detector diodes was measured and analyzed. The noise sources of amorphous silicon diodes were analyzed into three sources; shot noise, flicker noise and thermal noise from the contact resistance. By comparing the measured ENC with the calculated signal charge in uniform generation case, the signal-to-noise ratio (S/N) for the sample diodes is estimated as a function of the detector bias and the shaping time of Gaussian pulse shaper. The maximum S/N occurred at the bias level just above the full depletion voltage for shaping time of 2∼3 ${\mu}$sec. The developed method is useful in optimum design or amorphous silicon p-i-n diodes for charged particulate radiation spectroscopy.

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Source Identification and Reduction of Noise for the Outdoor Unit of Room Air Conditioner (실외기 소음원 규명 및 소음저감)

  • 서상호;이근동;이내영;진심원
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 1998.04a
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    • pp.575-581
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    • 1998
  • The noise sources in the outdoor unit of RAC(Room Air Conditioner) are identified by the sound intensity method. The main noise sources are compressor noise and fluid noise which is caused by the fan, heat exchanger and shroud. First, the fluid noise is reduced through the design ol new fan and shroud with high flow rate and low noise, reduction of the system resistance by rearrangement of heat exchanger, and optimization of the complex parameter between the fan and shroud. Next, in order to reduce the compressor noise, the new shape of compressor mount and sound-proof material was applied. As a result, the overall noise was reduced by 4-5dB(A).

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