• Tuberculosis and Respiratory Diseases
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• v.73 no.6
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• pp.312-319
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• 2012

Background: Muscle wasting in sepsis is associated with increased proteolysis. Interleukin-15 (IL-15) has been characterized as an anabolic factor for skeletal muscles. Our study aims to investigate the role of IL-15 in sepsis-induced muscle atrophy and proteolysis. Methods: Mice were rendered septic either by cecal ligation and puncture or by intraperitoneal injection of lipopolysaccharide (LPS, 10 mg/kg i.p.). Expression of IL-15 mRNA and protein was determined by reverse transcriptase polymerase chain reaction and Western blot analysis in the control and septic limb muscles. C2C12 skeletal muscle cells were stimulated in vitro with either LPS or dexamethasone in the presence and absence of IL-15 and sampled at different time intervals (24, 48, or 72 hours). IL-15 ($10{\mu}g/kg$) was intraperitoneally administered 6 hours before sepsis induction and limb muscles were sampled after 24 hours of sepsis. Cathepsin L activity was determined to measure muscle proteolysis. Atrogin-1 and muscle-specific ring finger protein 1 (MuRF1) expressions in limb muscle protein lysates was analyzed. Results: IL-15 mRNA expression was significantly lower in the limb muscles of septic mice compared to that of controls. Cathepsin L activity in C2C12 cells was significantly lower in presence of IL-15, when compared to that observed with individual treatments of LPS or dexamethasone or tumor necrosis factor ${\alpha}$. Further, the limb muscles of mice pre-treated with IL-15 prior to sepsis induction showed a lower expression of atrogin-1 and MuRF1 than those not pre-treated. Conclusion: IL-15 may play a role in protection against sepsis-induced muscle wasting; thereby, serving as a potential therapeutic target for sepsis-induced skeletal muscle wasting and proteolysis.

• Korean Journal of Microbiology
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• v.50 no.4
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• pp.334-344
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• 2014

The objective of this study was to evaluate the microbiological and physicochemical characteristics, and the antagonistic activity against Helicobacter pylori ATCC 43504, of yogurt fermented with the lactic acid bacteria from Baikkimchi kept under cold storage. The viable cell counts, titratable acidity, viscosity, and total solid content of the yogurt were different according to the bacterial strains used for fermentation. There was no significant change (P>0.05) in the various properties of refrigerated yogurt. Among the tested strains, the strongest resistance against artificial gastric juice and bile salt was found for Lactobacillus brevis BK11 and Lactobacillus paracasei BK57. Due to high lactic acid levels obtained from these two lactic acid bacteria, yogurt may show good anti-Helicobacter effects according to the time-kill assay. In particular, yogurt fermented with L. brevis BK11 significantly reduced the number of H. pylori adhering to gastric epithelial AGS cells and the urease activity of this pathogen (P<0.05).

• Preventive Nutrition and Food Science
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• v.11 no.1
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• pp.31-35
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• 2006

In order to improve the antiobesity effect of Kochujang, 1% of sea tangle powder, alginic acid extract, and fucoidan extract were added to Kochujang. Sea tangle powder-added Kochujang decreased leptin secretion by only 12% compared to Kochujang, whereas alginic acid or fucoidan-added Kochujang significantly decreased leptin secretion by more than 60% in 3T3-L1 adipocytes. Fucoidan, one of the active components of sea tangle, decreased leptin secretion by 56%, 60%, and 60% compared to the control in the concentrations of $1{\mu}M,\;2.5{\mu}M,\;and\;5{\mu}M$, respectively. To see the effect of fucoidan on TG formation during adipocyte differentiation, 3T3-L1 cells were treated with $1{\mu}M\;and\;5{\mu}M$ concentrations of fucoidan during adipocyte differentiation (from 'day 0' to 'day 6'). Oil red O staining showed fucoidan decreased the amount of TG droplets and $5{\mu}M$ fucoidan potently inhibited TG formation. To see the effect of fucoidan on lipolysis, differentiated 3T3-L1 adipocytes were treated with fucoidan. The secretion of glycerol, which is used to measure lipolytic activity, was increased by 21%, 37%, and 53% compared to the control in the concentrations of $1{\mu}M,\;2.5{\mu}M,\;and\;5{\mu}M$, respectively. Oil red O staining showed fucoidan decreased TG amount at $1{\mu}M\;and\;5{\mu}M$ concentrations. These results suggest that fucoidan decreases leptin secretion and TG accumulation by inhibition of adipocyte differentiation and induction of lipolysis. Since fucoidan is reported to have various biological activities in addition to an anti-adipogenic effect, it seems valuable to develop fucoidan-added Kochujang as a multi-functional Kochujang.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.9
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• pp.4274-4282
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• 2011

In this study, Fenton reaction was studied for the possibility of applying as advanced treatment and its optimal condition for the removal of refractory organics from the dye wastewater. Fenton reaction was applied to remove refractory organics after the bio-treatment (secondary treatment) inside test laboratory and on-site pilot plant. Wastewater from the secondary treatment was used and its $COD_{Mn}$ was measured as 30~50mg/L. After the Fenton reaction, the optimal condition was found as pH 3~3.5, reaction time 2~2.5hr, chemical input ratio of ($FeCl_2$(33%)/$H_2O_2$(35%)) was 3 : 1. When chemical input ratio of ($FeCl_2$(33%)/$H_2O_2$(35%)) was at its optimal, amount of sludge volume ($SV_{2hr}$) was 21~28%. With pilot plant test, removal rate was heavily influenced by the hydraulic retention time(HRT), and optimum value of HRT was 2.0 hr. When pilot plant($2m^3/d$) was placed on-site and operated continuously, it showed steady and fairly good treatment of COD where COD removal rate was 60~70%, treated water showed below 20mg/L.

• Journal of Digital Convergence
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• v.13 no.6
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• pp.165-175
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• 2015

Methods to design programs which implement IT systems have been developed in various forms from flowchart to activity diagram of UML. However, program design tools and methods developed so far have not been efficient comparing to program coding tools and methods. In addition, Program design methods and tools developed until now have been difficult to support the bidirectional conversion between program design and coding, and the improvement of development productivity and maintainability. Therefore, in this study, we propose Convergence Development Method to enable working with wide bandwidth through fusing the program design and coding phase by using SOC and supporting tool named SETL which automatizes the convergence of design and coding. Thus, by using SETL, it is expected that the efficiency gap between the program design and coding phase is reduced, and development productivity and maintainability is increased. key words.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.10 no.4
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• pp.259-265
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• 1977

After a great mortality owing to abnormal oceanographical condition and fungal disease in 1973 hanging cultch lines per raft has been reduced to 450 from 558, and oyster production per raft has also been decreased. It seems to be result of dense culture of oysters and its resulting accumulation of waste materials on the sea bottom in the farm area. The present study was carried out to investigate the effect of rearing density and the degree of eutrophication in Geoje Bay for 6 months from June through November in 1977. Total area of this bay is about $48.9\;km^2$, and the area of registered oyster farms as of November in 1977 is around $10.9\;km^2$, which is about $22.3\%$ of this bay. Water Quality during summer season was shown as transparency 5.5 m, COD 1.5ppm, degree of oxygen saturation $90.6\%$ in upper and middle layers and $82.2\%$ near bottom, chlorophyll-a 3.0 mg/m^3, and phytoplankton $8.7\times10^4\;cells/l$. In superficial mud in August COD was 35.4 mg/g, total sulphide 0.24 mg/g, and phaeophytin $43.7\;{\mu}g/g$dry mud. These values indicate that water qualify in this bay is so far excellent but quantities of chemical oxygen demend and sulphide in bottom mud show maximum level or a little over eutrophication standard.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.11 no.4
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• pp.243-247
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• 1978

COD and sulphide contents in the superficial mud in three oyster farms, one near Gajo-do in Chinhae Bay, the second near Eup-do in Koseong Bay and the third near Eogu in Keoje Bay, around the Chungmu area have been monthly determined for seven months from May through November in 1978. Bottom water was also checked for chlorophyll-a, dissolved oxygen, COD and sulphide. In general, large amounts of COD and sulphide in the superficial mud were found in the summer season, COD was 38.1 mg/g dry mud in the farm near Gajo-do, 32.3 mg/g near Eup-do and 25.1 mg/g in Eogu farm and sulphide was 0.313 mg/g dry mud, 0.517 mg/g and 0.132 mg/g respectively. COD and sulphide contents in the farms near Gajo-do and Eup-do were a little over the upper level of the eutrophic range, that is, 30 mg/g dry mud in COD and 0.3 mg/g dry mud in sulphide. It shows that the above two oyster farms were already eutrophicated. However, among three oyster farms no clear difference in bottom water was found.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.37 no.12
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• pp.18-23
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• 2000

In this paper, we propose the organic light-emitting devices with vacuum evaporated ultrathin CsF layer between the AI electrode and conjugated polymer MEH-PPV which was spin coated. In this structure, the CsF layer will be well transferred the electron injection from the electrode to the emission layer MEH-PPV. Finally this structure enhances the emission efficiency of the organic light-emitting device. And we measured the I-V-L properties with the split of CsF thickness into the $2{\AA},\;4{\AA},\;8{AA},\;10{\AA},\;20{\AA},\;50{\AA},\;75{AA}$ respectively. And also we evaporated CsF/Al, CsF/Au Cs/Au electrode respectively for the comparison. As the results, we obtained the maximum quantum efficiency 0.6% at $4{\AA}$ CsF thickness and then at $8{\AA}$, it decreased a little but it's still better than pure Al electrode which has 0.01%.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.36 no.5
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• pp.341-345
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• 2010

Introduction: Skeletal homeostasis is normally maintained by the stability between bone formation by osteoblasts and bone resorption by osteoclasts. However, the correlation between the inflammatory reaction and osteoblastic differentiation of cultured osteoprogenitor cells has not been fully investigated. This study examined the effects of inflammatory cytokines on the osteoblastic differentiation of cultured human periosteal-derived cells. Materials and Methods: Periosteal-derived cells were obtained from the mandibular periosteum and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured in an osteogenic induction Dulbecco's modified Eagle's medium (DMEM) medium containing dexamethasone, ascorbic acid, and $\beta$-glycerophosphate. In this culture medium, tumor necrosis factor (TNF)-$\alpha$ with different concentrations (0.1, 1, and 10 ng/mL) or interleukin (IL)-$1{\beta}$ with different concentrations (0.01, 0.1, and 1 ng/mL) were added. Results: Both TNF-$\alpha$ and IL-$1{\beta}$ stimulated alkaline phosphatase (ALP) expression in the periosteal-derived cells. TNF-$\alpha$ and IL-$1{\beta}$ increased the level of ALP expression in a dose-dependent manner. Both TNF-$\alpha$ and IL-$1{\beta}$ also increased the level of alizarin red S staining in a dose-dependent manner during osteoblastic differentiation of cultured human periosteal-derived cells. Conclusion: These results suggest that inflammatory cytokines TNF-$\alpha$ and IL-$1{\beta}$ can stimulate the osteoblastic activity of cultured human periosteal-derived cells.

• The KIPS Transactions:PartB
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• v.14B no.6
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• pp.431-436
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• 2007

Magnetic Resonance Imaging(MRI) has chemical shift phenomenon between fat and water, and the phenomenon has influence on structure enclosed by fat. Strong signals emitted from fat often generate false artefact, which reflects the importance of fat suppression techniques. There have been a number of researches on fat suppression techniques, but using fat suppression method alone in MRI can cause difficultproblems in diagnosis. This paper aims to study a fat suppression method by Chemical Shift Selective saturation(CHESS). This research describes the theoretical background and the experiment on water and fat phantom with MR instruments. In the experiment, CHESS pulse was designed by utilising Matlap program, and the pulse diagram was generated for the Pre-saturation process. The experiment using water and fat phantom was applied to C-spine, L-spine and Breast, and produced successful fat suppression results. This experiment has proved that the CHESSpulse fat suppression is a very helpful technique in diagnosing medical imaging. This method is a robust and useful technique for both clinical and basic investigators..(Experiment with Chungnam national university hospital G.E 1.5T MR)