Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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Evaluation of osteogenic activity of periosteal-derived cells treated with inflammatory cytokines

골막기원세포의 조골세포로의 분화과정에서 염증성 사이토카인의 효과

  • Park, Bong-Wook (Department of Oral and Maxillofacial Surgery, School of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Choi, Mun-Jeoung (Department of Oral and Maxillofacial Surgery, School of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Hah, Young-Sool (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Cho, Hee-Young (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Kim, Deok-Ryong (Biomedical center, Brain Korea 21 (BK21), Gyeongsang National University) ;
  • Kim, Uk-Kyu (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kang, Hee-Jea (Maxillofacial Center, Onhospital) ;
  • Kim, Jong-Ryoul (Maxillofacial Center, Onhospital) ;
  • Byun, June-Ho (Department of Oral and Maxillofacial Surgery, School of Medicine and Institute of Health Sciences, Gyeongsang National University)
  • 박봉욱 (경상대학교 의학전문대학원 구강악안면외과학교실) ;
  • 최문정 (경상대학교 의학전문대학원 구강악안면외과학교실) ;
  • 하영술 (경상대학교병원 임상의학연구소) ;
  • 조희영 (경상대학교병원 임상의학연구소) ;
  • 김덕룡 (경상대학교 건강과학연구원, 의생명과학사업단, Brain Korea 21 (BK21)) ;
  • 김욱규 (부산대학교 치의학전문대학원 구강악안면외과) ;
  • 강희제 (온종합병원 턱얼굴외과) ;
  • 김종렬 (온종합병원 턱얼굴외과) ;
  • 변준호 (경상대학교 의학전문대학원 구강악안면외과학교실)
  • Received : 2010.05.31
  • Accepted : 2010.10.13
  • Published : 2010.10.29
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Abstract

Introduction: Skeletal homeostasis is normally maintained by the stability between bone formation by osteoblasts and bone resorption by osteoclasts. However, the correlation between the inflammatory reaction and osteoblastic differentiation of cultured osteoprogenitor cells has not been fully investigated. This study examined the effects of inflammatory cytokines on the osteoblastic differentiation of cultured human periosteal-derived cells. Materials and Methods: Periosteal-derived cells were obtained from the mandibular periosteum and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured in an osteogenic induction Dulbecco's modified Eagle's medium (DMEM) medium containing dexamethasone, ascorbic acid, and $\beta$-glycerophosphate. In this culture medium, tumor necrosis factor (TNF)-$\alpha$ with different concentrations (0.1, 1, and 10 ng/mL) or interleukin (IL)-$1{\beta}$ with different concentrations (0.01, 0.1, and 1 ng/mL) were added. Results: Both TNF-$\alpha$ and IL-$1{\beta}$ stimulated alkaline phosphatase (ALP) expression in the periosteal-derived cells. TNF-$\alpha$ and IL-$1{\beta}$ increased the level of ALP expression in a dose-dependent manner. Both TNF-$\alpha$ and IL-$1{\beta}$ also increased the level of alizarin red S staining in a dose-dependent manner during osteoblastic differentiation of cultured human periosteal-derived cells. Conclusion: These results suggest that inflammatory cytokines TNF-$\alpha$ and IL-$1{\beta}$ can stimulate the osteoblastic activity of cultured human periosteal-derived cells.

Keywords

References

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