• 한국식품과학회지
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• 제37권4호
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• pp.656-659
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• 2005

신선초의 기능성 해명과 유용 기능성성분 탐색연구의 일환으로 신선초 지상부로부터 항산화활성물질을 분리하여 구조 해석하였다. 신선초 지상부의 열수추출물을 용매분획하여 ethyl acetate 가용 중성획분을 얻었다. 이를 Sephadex LH-20 column chromatography와 HPLC로 정제하여 항산화활성물질 2종을 단리하고, 그들 각각의 화합물을 대상으로 FAB-MS와 NMR 분석을 실시한 결과, pseudoisopsoralen과 8-methoxypsoralen(xanthotoxin)으로 동정하였다.

• Food Science and Biotechnology
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• 제18권3호
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• pp.766-770
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• 2009

Hot water extracts of Camellia japonica flower buds were found to have the higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than the other solvent extracts. Five phenolic compounds were isolated and purified from the ethyl acetate soluble-neutral fraction of hot water extracts by Sephadex LH-20 column chromatography and octadecyl silane-high performance liquid chromatography using the guided assay of DPPH radical scavenging. Based on mass spectrometer and nuclear magnetic resonance, the isolated compounds were identified as p-hydroxybenzaldehyde (1), vanillin (2), dehydroxysynapyl alcohol (3), 7S,7'S,8R,8'R-icariol $A_2$ (4), and (-)-epicatechin (5). Four compounds (1-4) except for 5 were newly identified in this plant. Their DPPH radical scavenging activities as 50% scavenging concentration decreased in order to 5 $(20\;{\mu}M)>{\alpha}-tocopherol$ $(29\;{\mu}M)>4$ $(67\;{\mu}M)>3$ $(72\;{\mu}M)>1=2$ ($>250\;{\mu}M$). These results indicate that the antioxidant effect of the hot water extract of C. japonica flower buds may partially due to 5 isolated phenolic compounds.

• 한국식품과학회지
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• 제42권6호
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• pp.755-761
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• 2010

본 연구에서는 녹두 및 대두의 용매추출물을 제조하고 hepatoma 1c1c7 세포 및 대식세포인 RAW264.7 배양을 이용하여 항암 및 항염증 효과를 비교분석하였다. 녹두추출물은 대두추출물 보다 ethylacetate 및 ethanol 추출물에서 모두 유의적으로 높은 QR 유도활성과 NO 및 $PGE_2$의 생성 억제 효과를 보였으며 농도 의존적 효과를 나타냈다. C18 silica flash column chromatography를 이용하여 회수된 녹두에탄올 추출물의 7개 분획 중 소수성이 가장 강한 분획은 24 ${\mu}g$/mL의 CD value와 783 ${\mu}g$/mL의 $IC_{50}$를 보였다. 현재 다수의 염증 억제물질의 작용기전이 prostaglandin 합성 억제이며 이는 COX-2의 생성 및 활성저해에 의한 것임을 근거로 할 때 녹두의 ethanol 추출물은 항염증 및 암 발생 예방 성분을 분리하기 위한 중요한 식품 자원으로 생각된다.

• 한국약용작물학회지
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• 제28권4호
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• pp.245-253
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• 2020

Background: Research on hot water extracts of medicinal plants that are easily applicable in the clinical setting is essential. To confirm the anti-inflammatory-related active compounds present in the hot water extract of Eriobotrya japonica leaves, ability to inhibit nitric oxide (NO) production was measured and active compounds isolated from the extract were analyzed. Methods and Results: Sovent fractionation by solvent was performed to identify the active compounds present in the hot water extract, and the ability of the extract and the fractions obtained to inhibit NO production was measured. Subsequently, based on the results of liquid chromatography (LC) profile analysis of the n-butanol fraction that had a relatively high inhibitory ability of NO production, six subfractions were separated around the main peak. Among the separated subfractions spectra from mass spectroscopy (MS) were analyzed and standard comparisons were performed on the compounds of the three main peaks on the chromatogram. NO production inhibitory activity of subfraction 2 identified as neochlorogenic acid was the highest with an IC₅₀ of 18.49 ㎍/㎖ followed by that of subfraction 5 identified as cryptochlorogenic acid with IC₅₀ of 25.82 ㎍/㎖. Conclusions: Our result, it was confirmed that several caffeoylquinic acids, including neochlorogenic acid and cryptochlorogenic acid present in the hot water extract of E. japonica leaves have an important role as compounds exhibiting anti-inflammatory activity.

• 한국자원식물학회지
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• 제31권6호
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• pp.695-703
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• 2018

Previously, we have reported a plant extract isolated from Lysimachia foenum gracum Herba as a new environment friendly biopesticide that has the mycelial growth inhibition effect on Magnaporthe oryzae, the pathogenic fungus of the rice blast disease. For the finding of additional biopesticide candidate, we tested the mycelial growth inhibitory effects about 700 species of plant extracts on PDA media. Among them, the extract of Anemarrhena asphodeloides showed prominent inhibitory effect of which $IC_{50}$ was $139.7{\mu}g/ml$. Mycelial radii of M. oryzae were measured on PDA medium containing the four organic solvent fractions isolated from total extract from A. asphodeloides. Ethyl acetate fraction showed the impressive inhibitory effect of $IC_{50}$, $54.12{\mu}g/ml$. In the subsequent rice field test for the total extract of A. asphodeloides, we obtained encouraging 62.0% control rate of rice blast disease without any phytotoxicity. It is almost equivalent to that of chemical pesticides implying the applicability of the extract as a new biopesticide. In further study, the analysis of active ingredients of the extract would be necessary for the development of a new biopesticide and for the verification of cellular mechanism by which the mycelial growth of M. oryzae inhibited.

• Journal of the Korean Wood Science and Technology
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• 제30권4호
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• pp.66-73
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• 2002

느릅나무 근피와 점액질의 화학 조성을 분석하였고, 근피의 MeOH추출물을 각종 유기용제 및 수층으로 분획하고 각 분획물의 항균성을 조사하였다. 원소조성은 수종간의 차이는 없었지만, 목부의 질소 및 리그닌 함량은 사부보다 높았으며 회분 및 추출물양은 목부보다 사부가 높게 나타났다. 근피의 점액질은 냉수에 의해 매우 느리게 용출 되었으며, 높은 산성당 함량을 갖는 pectin 유사 물질이었다. 느릅나무 근피로부터 약 7% 정도의 메탄올 추출물이 얻어졌고 이 중 78%가 수용성 물질이었으며 Staphylococcus aureus와 Salmonella typhimurium에 대하여 항균성을 나타내었다.

• 한국식생활문화학회지
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• 제24권6호
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• pp.749-755
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• 2009

We investigated the chemical components of red onion powder dried using the low temperature vacuum method and the inhibitory effects of solvent extracts of the dried red onion powder on the growth of HT-1080 human fibrosarcoma and HT-29 human colon cancer cells and $H_2O_2$-induced oxidative stress. The moisture content of the dried red onion powder was 17.95%, while the vitamin C content was 96 mg/100 g and the total phenols content was 39.1 mg/mL. The inhibitory effects of acetone with methylene chloride (A+M) and methanol (MeOH) extracts of the red onion powder on the growth of HT-1080 and HT-29 cancer cells increased in a dose dependent manner (p<0.05). The inhibitory effect was greater on the growth of HT-29 cells, while the A+M extracts had a higher inhibitory effect than the MeOH extracts. Treatment with the hexane, 85% aq. methanol, butanol and water fractions of the extract led to significant inhibition of the growth of both cancer cell lines (p<0.05). Among the fractions, the hexane and 85% aq. methanol fractions showed a greater inhibitory effect. To determine the protective effect on $H_2O_2$-induced oxidative stress, a DCFH-DA (dichlorodihydrofluorescin diacetate) assay was conducted. All fractions, including the crude extracts of dried red onion, appeared to lead to a significant reduction in the levels of intracellular reactive oxygen species (ROS), and these reductions occurred in a dose dependent fashion (p<0.05). Among the fractions, the 85% methanol fraction showed the greatest protective effect on the production of lipid peroxides.

• Parasites, Hosts and Diseases
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• 제61권4호
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• pp.405-417
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• 2023

Chagas disease, caused by Trypanosoma cruzi parasite, is a significant but neglected tropical public health issue in Latin America due to the diversity of its genotypes and pathogenic profiles. This complexity is compounded by the adverse effects of current treatments, underscoring the need for new therapeutic options that employ medicinal plant extracts without negative side effects. Our research aimed to evaluate the trypanocidal activity of Bidens pilosa fractions against epimastigote and trypomastigote stages of T. cruzi, specifically targeting the Brener and Nuevo León strains-the latter isolated from Triatoma gerstaeckeri in General Terán, Nuevo León, México. We processed the plant's aerial parts (stems, leaves, and flowers) to obtain a methanolic extract (Bp-mOH) and fractions with varying solvent polarities. These preparations inhibited more than 90% of growth at concentrations as low as 800 ㎍/ml for both parasite stages. The median lethal concentration (LC₅₀) values for the Bp-mOH extract and its fractions were below 500 ㎍/ml. Tests for cytotoxicity using Artemia salina and Vero cells and hemolytic activity assays for the extract and its fractions yielded negative results. The methanol fraction (BPFC3MOH1) exhibited superior inhibitory activity. Its functional groups, identified as phenols, enols, alkaloids, carbohydrates, and proteins, include compounds such as 2-hydroxy-3-methylbenzaldehyde (50.9%), pentadecyl prop-2-enoate (22.1%), and linalool (15.4%). Eight compounds were identified, with a match confirmed by the National Institute of Standards and Technology (NIST-MS) software through mass spectrometry analysis.

• Weed & Turfgrass Science
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• 제2권3호
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• pp.242-247
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• 2013

현재 유기재배에서 잡초 방제를 위한 방법은 관행방법에 비해 효과적이지 못하다. 본 연구는 하늘타리의 잎, 줄기, 열매, 뿌리 추출물 중 어느 부위에서 제초 효과가 가장 높은지를 알아보기 위해서 각 부위별로 물, 열수 그리고 에탄올로 추출하였다. 또한 위의 추출방법 중 제초활성능력이 뛰어난 것을 hexane, chloroform, ethyl acetate, BuOH, $H_2O$로 용매분획하여 잠재적 제초 성분에 대한 특성을 조사하였다. 전반적으로 오이와 보리의 발아율, 초장 및 근장 억제는 추출방법 중 물 추출법이 가장 효과적이었고, 식물체 부위별로는 열매에서 가장 효과적이었다. Ethyl acetate, butanol, chloroform, hexane, water의 용매분획에서 오이와 보리의 발아율, 초장 및 근장 저해는 물 층에서 가장 좋았고 그 밖의 분획에서는 차이가 없었다. 5% 물 층 추출물 발아전 처리에 의해 바랭이와 까마중의 생장량은 80~100% 억제되었으나, 이 추출물에 의한 오이와 보리뿐만 아니라 잡초종(바랭이, 까마중 등) 경엽처리에 의한 생육저해 효과는 없었다. 본 연구결과로 볼 때 하늘타리 추출물을 친환경 또는 유기농 재배에 잡초방제를 위해 사용할 수 있을 것으로 본다.

• 한국식품과학회지
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• 제10권2호
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• pp.263-268
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• 1978

원료삼(原料蔘) 종류(種類)에 따른 각(各) 인삼제제품(人蔘製劑品)의 성분(成分) 함량(含量)과 품질관리(品質管理)에 기초자료(基礎資料)를 얻고자 인삼(人蔘) 부위별(部位別) 및 연근(年根)에 따른 기초성분(基礎成分)과 배당체(配糖體) 성분(成分) 함량(含量) 및 무기성분(無機成分)을 분석(分析) 비교(比較)한바 그 결과(結果)는 다음과 같다. 1. 인삼(人蔘) 부위별(部位別) 일반성분(一般成分) 비교(比較) 결과(結果) 회분(灰分), 조섬유(粗纖維)는 뇌두(腦頭), 표피(表皮), 세미(細尾)에 많았고 조단백(粗蛋白)과 전당(全糖)은 동체(胴體)(중심부(中心部))와 지근(枝根)에 많았다. 2. 연근별(年根別) 인삼(人蔘)의 일반성분(一般成分)에 있어서 회분(灰分)은 저연근(低年根)인 2,3연근(年根)에 많은 편(便)이고 조섬유(粗纖維), 전당(全糖)은 고연근(高年根)에 많은 경향(傾向)이었으며 조지방(粗脂肪), 조단백(粗蛋白)은 연근별(年根別) 뚜렷한 경향(傾向)이 없었다. 3. 인삼부위별(人蔘部位別) alcohol extract 및 saponin 함량(含量)은 표피(表皮), 세미(細尾) 뇌두부위(腦頭部位)가 동체(胴體)(주근부(主根部))와 지근(支根)에 비(比)하여 많았다. 부위별(部位別) 인삼(人蔘) saponin fraction 비교결과(比較結果) 각(各) 부위별(部位別)로 saponin 개개(箇箇) 함유비율(含有比率)이 차이(差異)가 있었는데 동체(胴體)와 지근(支根)은 뇌두(腦頭), 표피(表皮), 세미(細尾)에 비(比)하여Paxacatriol/Panaxadiol의 비율(比率)이 상대적(相對的)으로 높았다. 4. 연근별(年根別) alcohol extract 수율(收率)은 3,4연근(年根)에 saponin 5,6은 연근(年根)에 다소(多少) 많은 경향(傾向)이었다. 5. 추출(抽出) 용매(溶媒)에 따른 extract 수율(收率)은 물 추출구(抽出區)가 높고 alcohol의 농도(濃度)가 높을수록 낮아지는 경향(傾向)이었다. alcohol추출(抽出) 농도(濃度)에 따른 extract는 성분함량(成分含量)과 물리성(物理性)도 차이(差異)가 많았다. 6. 무기성분중(無記性分中) T-N, $P_2O_5,\;K_2O$는 저연근(低年根)인 $2{\sim}3$연근(年根)에 $SiO_2,\;CaO$는 고연근(高年根)에 많은 경향(傾向)이었다. 기타(其他) MgO, Fe, Zn, Na등(等)은 연근별(年根別) 뚜렷한 경향(傾向)이 없었다.