Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Nitric Oxide Production Inhibitory Effects of Three Caffeoylquinic Acids Isolated from Hot Water Extract of Eriobotrya japonica L. Leaves

비파엽 열수 추출물에서 분리한 Caffeoylquinic Acid 3 종의 Nitric Oxide 생성 억제 효과

  • 김선민 (동신대학교 한약재산업학과) ;
  • 김아영 (동신대학교 바이오센터) ;
  • 이경인 (동신대학교 바이오센터)
  • Received : 2020.06.02
  • Accepted : 2020.08.25
  • Published : 2020.08.30
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Abstract

Background: Research on hot water extracts of medicinal plants that are easily applicable in the clinical setting is essential. To confirm the anti-inflammatory-related active compounds present in the hot water extract of Eriobotrya japonica leaves, ability to inhibit nitric oxide (NO) production was measured and active compounds isolated from the extract were analyzed. Methods and Results: Sovent fractionation by solvent was performed to identify the active compounds present in the hot water extract, and the ability of the extract and the fractions obtained to inhibit NO production was measured. Subsequently, based on the results of liquid chromatography (LC) profile analysis of the n-butanol fraction that had a relatively high inhibitory ability of NO production, six subfractions were separated around the main peak. Among the separated subfractions spectra from mass spectroscopy (MS) were analyzed and standard comparisons were performed on the compounds of the three main peaks on the chromatogram. NO production inhibitory activity of subfraction 2 identified as neochlorogenic acid was the highest with an IC50 of 18.49 ㎍/㎖ followed by that of subfraction 5 identified as cryptochlorogenic acid with IC50 of 25.82 ㎍/㎖. Conclusions: Our result, it was confirmed that several caffeoylquinic acids, including neochlorogenic acid and cryptochlorogenic acid present in the hot water extract of E. japonica leaves have an important role as compounds exhibiting anti-inflammatory activity.

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References

  1. Bae YI, Jeong CH and Shim KH. (2002). Nitrite-scavenging and antimutagenic effects of various solvent extract from different parts of loquat(Eriobotrya japonica Lindl.). Korean Journal of Food Preservation. 9:92-96.
  2. Bae YI, Seo KI, Park SK and Shim KH. (1998). Loquat (Eriobotrya japonica Lindl.) leaf tea processing and its physicochemical properties. Korean Journal of Postharvest Science and Technology. 5:262-269.
  3. Bajko E, Kalinowska M, Borowski P, Siergiejczyk L and Lewandowski W. (2016). 5-O-Caffeoylquinic acid: A spectroscopic study and biological screening for antimicrobial activity. LWT-Food Science and Technology. 65:471-479. https://doi.org/10.1016/j.lwt.2015.08.024
  4. Cha DS, Eun JS and Jeon H. (2011). Anti-inflammatory and antinociceptive properties of the leaves of Eriobotrya japonica. Journal of Ethnopharmacology. 134:305-312. https://doi.org/10.1016/j.jep.2010.12.017
  5. Cho EK and Choi YJ. (2011). Antioxidant and anti-inflammatory activities of extracts of traditional medicinal plants mixtures 1 and 2. Journal of Life Science. 21:596-603. https://doi.org/10.5352/JLS.2011.21.4.596
  6. Ding A, Nathan CF and Stuehr DJ. (1988). Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages: Comparison of activating cytokines and evidence for independent production. The Journal of Immunology. 141:2407-2412.
  7. Gao X, Zhang S, Wang L, Yu L, Zhao X, Ni H, Wang Y, Wang J, Shan C and Fu Y. (2020). Anti-inflammatory effects of neochlorogenic acid extract from mulberry leaf(Morus alba L.) against LPS-stimulated inflammatory response through mediating the AMPK/Nrf2 signaling pathway in A549 cells. Molecules. 25:1385. https://doi.org/10.3390/molecules25061385 (cited by 2020 March 18).
  8. Huang Y, Li J, Wang R, Wu Q, Li Y, Yu S, Cheng W and Wang Y. (2007). Effect of triterpene acids of Eriobotrya japonica(Thunb.) Lindl. leaf on inflammatory cytokine and mediator induction from alveolar macrophages of chronic bronchitic rats. Inflammation Research. 56:76-82. https://doi.org/10.1007/s00011-006-5185-0
  9. Hwang YG, Lee JJ, Kim AR and Lee MY. (2010). Chemical components and antioxidative effects of Eriobotrya japonica Lindl. leaf. Journal of Life Science. 20:1625-1633. https://doi.org/10.5352/JLS.2010.20.11.1625
  10. Kang CH, Kwak DY and So JS. (2013). Inhibition of nitric oxide production and hyaluronidase activities from the combined extracts of Platycodon grandiflorum, Astragalus membranaceus, and Schisandra chinensis. Journal of the Korean Society of Food Science and Nutrition. 42:844-850. https://doi.org/10.3746/jkfn.2013.42.6.844
  11. Kim IG and Kim KM. (2013). The optimal addition level of loquat leaf extract in the rice beverages. Korean Journal of Food and Nutrition. 26:226-233. https://doi.org/10.9799/ksfan.2013.26.2.226
  12. Kim MN, Choi SY, Lee PJ and Hur JY. (2015). Neochlorogenic acid inhibits lipopolysaccharide-induced activation and proinflammatory responses in BV2 microglial cells. Neurochemical Research. 40:1792-1798. https://doi.org/10.1007/s11064-015-1659-1
  13. Kim SH, Park SY, Park YL, Myung DS, Rew JS and Joo YE. (2017). Chlorogenic acid suppresses lipopolysaccharide-induced nitric oxide and interleukin-$1{\beta}$ expression by inhibiting JAK2/ STAT3 activation in RAW264.7 cells. Molecular Medicine Reports. 16:9224-9232. https://doi.org/10.3892/mmr.2017.7686
  14. Kim TM, Paudel KR and Kim DW. (2020). Eriobotrya japonica leaf extract attenuates airway inflammation in ovalbumininduced mice model of asthma. Journal of Ethnopharmacology. 253:112082. https://doi.org/10.1016/j.jep.2019.112082 (cited by 2020 May 10).
  15. Lee H, Park YJ and Lee JJ. (2017a). Protective effects of loquat(Eriobotrya japonica Lindl.) leaf extract on ethanolinduced liver damage in rats. The Korean Journal of Community Living Science. 28:537-546. https://doi.org/10.7856/kjcls.2017.28.4.537
  16. Lee JY, Yoo DH, Joo DH, Kim SR, Jo HS, Joo SH and Chae JW. (2017b). Anti-inflammatory effects of Amelanchier asiatica fruits ethanol extract. Journal of the Society of Cosmetic Scientists of Korea. 43:19-26. https://doi.org/10.15230/SCSK.2017.43.1.19
  17. Lee KH, Jang JH, Woo KW, Nho JH, Jung HK, Cho HW, Yong JH and An BK. (2019). Anti-inflammatory effects of aurantio-obtusin isolated from Cassia tora L. in RAW264.7 cells. Korean Journal of Pharmacognosy. 50:11-17.
  18. Miyataka M, Rich KA, Ingram M, Yamamoto T and Bing RJ. (2002). Nitric oxide, anti-inflammatory drugs on renal prostaglandins and cyclooxygenase-2. Hypertension. 39:785-789. https://doi.org/10.1161/hy0302.105689
  19. Park JO, Park JO and Joo CG. (2015). A study on whitening and anti-inflammatory effects of Eriobotrya japonica leaf extracts with different extraction methods. Journal of the Society of Cosmetic Scientists of Korea. 41:151-157. https://doi.org/10.15230/SCSK.2015.41.2.151
  20. Rang MJ. (2013). Anti-inflammatory and anti-allergic effects of herbal extracts on atopic dermatitis(part II). Journal of the Korean Applied Science and Technology. 30:173-182.
  21. Shin KM, Park YM, Kim IT, Hong SP, Hong JP and Lee KT. (2003). In vitro antiinflammatory activity of amygdalin in murine macrophage Raw264.7 cells. Korean Journal of Pharmacognosy. 34:223-227.
  22. Whang TE, Lim HO and Lee JW. (1996). Anticancer effect of Eriobotrya japonica Lindl. by specificity test with several cancer cell lines. Korean Journal of Medicinal Crop Science. 4:314-320.
  23. Yoon CS, Kim DC, Ko WM, Kim KS, Lee DS, Kim DS, Cho HK, Seo JW, Kim SY, Oh HC and Kim YC. (2014). Anti-neuroinflammatory effects of quercetin-3-O-glucuronide isolated from the leaf of Vitis labruscana on LPS-induced neuroinflammation in BV2 cells. Korean Journal of Pharmacognosy. 45:17-22.
  24. Zhang W, Zhao X, Sun C, Li X and Chen K. (2015). Phenolic composition from different loquat(Eriobotrya japonica Lindl.) cultivars grown in China and their antioxidant properties. Molecules. 20:542-555. https://doi.org/10.3390/molecules20010542