• Journal of Animal Science and Technology
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• v.51 no.6
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• pp.527-536
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• 2009

A potent protein degrading bacterium was isolated from soil samples of different environments. Polyphasic taxonomic studies and phylogenetic 16S rRNA sequence analyses led to identify the isolate IB No. 11 as a strain of Bacillus subtilis. The isolated strain was recognized to produce protease constitutively, and the maximum production (1.64 units/ml) was attained in a shake flask culture when the isolate was grown at $40^{\circ}C$, for 32 h in basal medium supplemented with starch (0.25%) and gelatin (1.25%) as sole carbon and nitrogen source, respectively. The optimum pH and temperature for the protease activity were determined to be pH 7.0 and $50^{\circ}C$, respectively. $Ca^{2+}$ and $Mn^{2+}$ enhanced remarkably the protease activity but neither showed positive effect on the protease's thermal stability. In addition, it was observed that the protease was fairly stable in the pH range of 6.5-8.0 and at temperatures below $50^{\circ}C$, and it could be a good candidate for an animal feed additive. The inhibition profile of the protease by various inhibitors indicated that the enzyme is a member of serine-proteases. A combination of UV irradiation and NTG mutagenesis allowed to develop a protease hyper-producing mutant strain coded as IB No. 11-4. This mutant strain produced approximately 3.23-fold higher protease activity (6.74 units/mg) than the parent strain IB No. 11 when grown at $40^{\circ}C$ for 32h in the production medium. The protease production profile of the selected mutants was also confirmed by the zymography analysis.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.2
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• pp.16-25
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• 2007

The purpose of the study is to analyse and classify the characteristics of Gongsanseong forest through phytosociological and quadrat method with 20 plot samples. In result, the forest was classified as Quercus acutissima community. The subcommunity was composed of Quercus aliena and Zelkova serrata. The average vegetation coverage of Quercus aliena subcommunity was upper tree layer (83%), lower tree layer (48%), shrub layer (39%), and herb layer (30%), while the average vegetation coverage of Zelkova serrata subcommunity was upper tree layer (76%), lower tree layer (52%), shrub layer (40%), and herb layer (45%). The order of importance value of the forest community with DBH 2cm above plants was Quercus acutissima (72.62), Quercus aliena (40.52), Prunus sargentii (19.81), Styrax japonica (19.39), Zelkova serrata (15.78), Robonia pseudoacacia (14.76), Quercus variabilis (13.83), Sorbus alnifolia (13.71), Platycarya strobilacea (10.74), Pinus densiflora (10.08), and Quercus serrata (9.31). According to breast diameter analysis results, it is expected that the importance value of Prunus sargentii and Zelkova serrata will be continuously increased while Quercus sp. is dominating the forest. With the result of ordination analysis, the relationship between the forest community and environmental factors could be affected by soil nutrition, moisture, and elevation etc.

• Journal of Korean Society for Atmospheric Environment
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• v.16 no.1
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• pp.11-22
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• 2000

Polycyclic aromatic hydrocarbons(PAHs) have known as potentially hazardous air pollutants(HAPs0 to human health because of its carcinogenic and mutagenic behaviors. The purpose of this study was to determine the level of 6 PAHs(Fluoranthene, Pyrene, Benzo[a]anthracene, Chrysene, Benzo[b]fluoranthene, and Benzo[a]pyrene) as well as 10 inorganic elements(Cr, Na, K, Zn, Pb, Fe, Cu, Ti, Al and Cd) in the ambient PM-10. The total of 115 samples had been collected from February, 1996 to June, 1996 on quartz fiber by a PM-10 high volume air sampler near the Yong-Tong Apartment complexes. A statistical analysis was performed for the PAHs and inorganic elements data set using a principal component analysis in order to identify qualitatively the potential sources of PM-10. A total of 6 principal components were separated by intensive data pretrement and transformation processes, such as soil, refuse incineration, oil burning, coal burning, field burning, vehicle emission sources. The results showed that PAHs were associated with various burning activities like refuse and field burning, coal burning, and oil burning emissions in the study area. These derived sources were well matched with the previously known source profiles in terms of compositonal order and level of measured species. The combination data set consisted of both organic and inorganic species might provide more powerful source signature and might increase the number of potentially derived sources than PAHs or inorganic data alone.

• Journal of Life Science
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• v.14 no.2
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• pp.320-324
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• 2004

The objective of this study was investigate the characteristic of biosurfactant produced from the isolated strain. The strain was isolated from soil samples and identified as Bacillus sp. TBM 911-5 by physiological characteristics and the partial nucleotide sequence analysis of 16S rDNA. We measured the surface tension every 6 hours for 80 hours. The surface tension of the culture filtrate of Bacillus sp. TBM 911-5 was decreased to 29 mN/m. Biosurfactant concentration was determined by diluting the culture filtrate until the critical micelle concentration (CMC). The biosurfactant emulsified hydrocarbons, vegetable oil and crude oil. Using soybean oil as substrate, the maximum emulsification activity and stability was obtained from the biosurfactant. The biosurfactant produced from Bacillus sp. TBM 911-5 had strong properties as an emulsifying agent and an emulsion-stabilizing agent.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.03a
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• pp.278-287
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• 2009

This thesis studied effect of zoning on seepage flow in concrete faced gravel-fill dam (CFGD) designed to have selected main rockfill or gravel-fill zone, Zone 3Bs with higher permeability to enhance the safety against accidental water infiltration into the dam. For this purpose, centrifuge model tests with two cases, with and without Zone 3Bs, were performed in order to investigate the necessity and the function of Zone 3Bs. Model dams were made by soil samples with modified coefficients of permeability and concrete faced slab was simulated with aluminum alloy. Water infiltration was simulated by rising water table over cracks on the facing. Behaviors of model dams were measured by LVDTs, strain gages, pore water pressures and cameras. Form the results of centrifuge tests, it was found out that the Zone 3Bs acts as a protection of main gravel-fill zone by inducing flow paths for infiltrated water into it as well as by draining off the infiltrated water out of the dam in a short time.

• KSBB Journal
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• v.18 no.6
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• pp.490-494
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• 2003

For the production of antifungal compound, strain B-1 was used as a strong producing strain among bacteria isolated from various soil samples. The optimum medium for the production of antifungal compound was PDB (potato starch 0.4%, dextrose 2％, pH5.1). The optimum conditions for the production of antifungal compound didn't affect on the carbon and nitrogen sources. The produced compound showed broad antimicrobial activity to the tested strains such as five fungi and four bacteria. The optimum pH and temperature of the production antifungal compound were pH 5.0 and 28$^{\circ}C$, respectively. Ether extrct (1$\mu\textrm{g}$/${\mu}\ell$) of culture broth was confirmed inhibitory zone by the thin layer chromatography and plate assay. The antimicrobial compound was unstabled after heat (121$^{\circ}C$) trsatment. Strain B-1 was mass cultured in a 5-liter tormentor, containing 3 liters of PDB medium at 28$^{\circ}C$, pH 5.0, 120 (pm with aeration (1L/min).

• Journal of the Korean GEO-environmental Society
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• v.14 no.1
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• pp.61-68
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• 2013

This paper presents an experimental results on the environmental characteristics of waste tire. Experimental program includes a set of laboratory leaching tests and field pilot test for leachate analysis. Laboratory tests were conducted to illustrate how properties such as TOC, pH, turbidity and Zn change with tire sizes and drain conditions. In field pilot test, water samples were collected form a drainage system installed below the tire-reinforced retaining wall and analyzed for chemical quality. Laboratory leaching tests performed on various particle sizes of waste tire indicated that as tire size is increased, the concentration of leachate is decreased. In continuous flow column tests, the concentration of leachate decreased with the number of exposure periods or pore volumes flushed through the waste tire. However, during pause flow column tests, the concentration of leachates were increased with time. Field monitoring of effluent indicated that no significant adverse effects on ground water quality had occurred over a period of 12 months.

• Journal of the Korean Geotechnical Society
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• v.20 no.2
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• pp.97-105
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• 2004

The purpose of this study is to provide the ways of recycling of by-product limes as lightweight fill, backfill materials, and lightweight blocks by performing experimental study. New lightweight fill materials and blocks were devised by mixing by-product lime, weathered granite soil, small pieces of waste EPS, and Portland cement. Physical, geotechnical, and environmental properties of the lightweight mixed soils and blocks were analysed by laboratory experiments for mixed samples manufactured with various mixing ratios. KMS tests were also performed to evaluate the concentration variation of the chemical components of the light weight blocks leachates. It is expected that this study will contribute to resolving the problem of by-product lime disposal as well as to recycling the by-product limes as fill materials and blocks.

• Journal of Species Research
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• v.5 no.2
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• pp.223-234
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• 2016

As a subset work for the collection of indigenous prokaryotic species in Korea, 38 actinobacterial strains were isolated from various environmental samples obtained from plant root, ginseng cultivating soil, mud flat, freshwater and seawater. Each strain showed higher 16S rRNA gene sequence similarity (>99.1%) and formed a robust phylogenetic clade with closest actinobacterial species which were defined and validated with nomenclature, already. There is no official description on these 38 actinobacterial species in Korea. Consequently, unrecorded 37 species of 24 genera in the 12 families belonging to the order Actinomycetales of the phylum Actinobacteria were found in Korea. Morphological properties, basic biochemical characteristics, isolation source and strain IDs are described in the species descriptions.

• 한국지구물리탐사학회:학술대회논문집
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• 2008.10a
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• pp.37-40
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• 2008

We have measured resistivities for undisturbed soil samples collected from dredged bank with sea sand, and analyzed with one-dimensional inversion results from small-loop electromagnetic survey data. From the relationship between the two resistivities, it appeared that calculated resistivities were remarkably consistent with measured resistivities. Correlation relationships between resistivity values and cone resistance were analyzed after comparing inversion results with cone resistance. It turns out that the region with below 1 ohm-m is correspondent to that of with less than $50\;kgf/cm^2$ in dredged bank with sea sand. From the study result, resistivity monitoring of small-loop EM periodically is proved to be more effective to maintain the stability of embankment dike.