• Title/Summary/Keyword: soil nitrate

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Environmental Effect on the Biodegradation of Toluene by Pseudomonas fluorescence KNU417 (원유오염 토양으로부터 분리한 Pseudomonas fluorescence KNU417의 톨루엔 분해에서 환경 인자의 영향)

  • Kwon, Hyeok-Man;Yeom, Sung-Ho
    • Journal of the Korea Organic Resources Recycling Association
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    • v.14 no.3
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    • pp.117-125
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    • 2006
  • A microorganism capable of degrading toluene was isolated from crude oil contaminated soil and identified as Pseudomonas fluorescence. The effects of environmental factors on the degradation of toluene were investigated. The optimum temperature for toluene degradation was $30^{\circ}C$ and the maximum specific cell growth and toluene degradation rates were $0.76hr^{-1}$ and $0.36hr^{-1}$, respectively. Although the wild cells were not able to degrade toluene at $10^{\circ}C$ and $40^{\circ}C$, the cells adapted to toluene at $30^{\circ}C$ degraded 100mg/L of toluene completely at $10^{\circ}C$ and 80% of the toluene at $40^{\circ}C$. The wild cells were not able to degrade more than 200mg/L of toluene but the toluene-adapted cells degraded up to 300mg/L of toluene. Although the optimum pH was 7.0, the degradation rates were not much different in the range of 5.5 to 9.0. When nitrate was used as a nitrogen source instead of ammonium, the adaptation period became longer by 2~10 hours and the cell growth yield became lower by 45%. The toluene degradation rates after adaptation period, however, were almost same in both cases. The observations in this study will be used in the future biofilter design and operation.

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Characteristics of Chemical Species in Gaseous and Aerosol Phase Measured at Gosan, Korea During ABC-EAREX2005 (ABC-EAREX2005 기간 동안 제주도 고산지역 대기 중 가스상 및 입자상 물질의 분포특성)

  • Moon K.J.;Han J.S.;Kong B.J.;Lee M.D.;Jung I.R.
    • Journal of Korean Society for Atmospheric Environment
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    • v.21 no.6
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    • pp.675-687
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    • 2005
  • In this study, the cyclone/denuder/filter pack sampling system was used to measure the daily concentrations of water soluble inorganic compounds of fine ($D_{p}< 2.5\;{\mu}m$) and coarse ($D_{p}<10{\mu}m$m) size fractions of aerosol and related gases at Gosan super site during ABC-EAREX 2005. The mean concentrations for $HNO_{3},\;HNO_{2},\;NH_{3}$, were 0.39, 0.08, and $0.29\;{\mu}g/m^3$. respectively. Average concentrations of sulfate, nitrate, and ammonium in $PM_{2.5}$ were 3.39, 1.06, and $1.04\;{\mu}g/m^3$, which occupied about $26\%$ of total $PM_{2.5}$ mass. In particular, more than half of these ionic species were found in size of less than $2.5\;{\mu}m$. Gas phase nitric acid concentrations have shown high correlation coefficient with $HNO_{2}$(R=0.80) and $O_{3}$(R=0.78), implying the active photochemical reactions from its precursors. Equivalent molar ratios between major ion components, $NH_{4}\;^{+}/nss\;SO_4\;^{2-},(0.83\;for\;PM_{2.5},\;0.86\;for\;PM_{10})$, revealed that the existing forms of the secondary aerosols were probably $(NH_{4})_{2}SO_{4}\;and\;(NH_{4})_{3}H\;(SO_{4})_{2}$. Especially, $(NH_{4}\;^{+}+K^{+}+Ca^{2+}+Mg^{2+})/(NO_{3}\;^{-}+nss\;SO_{4}\;^{2-}) (0.99\;for\;PM_{2.5},\;1.05\;for\;PM_{10})$ indicated that some of them existed not only as $NH_{4}NO_{3}$ but also as $CaSO_{4}\;or\;KNO_{3}$, which pointed out the probability of influences from the abundant soil components during Asian dust (AD) periods. These neutralized types of secondary aerosols showed that pollutants could be aged and transported from a distance.

Identification of Atmospheric PM10 Sources and Estimating Their Contributions to the Yongin-Suwon Bordering Area by Using PMF (PMF모델을 이용한 용인.수원 경계지역에서 PM10 오염원의 확인과 상대적 기여도의 추정)

  • Lee, Hyung-Woo;Lee, Tae-Jung;Yang, Sung-Su;Kim, Dong-Sool
    • Journal of Korean Society for Atmospheric Environment
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    • v.24 no.4
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    • pp.439-454
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    • 2008
  • The purpose of this study was to extensively identify $PM_{10}$ sources and to estimate their contributions to the study area, based on the analysis of the $PM_{10}$ mass concentration and the associated inorganic elements, ions, and total carbon. The contribution of $PM_{10}$ sources was estimated by applying a receptor method because identifying air emission sources were effective way to control the ambient air quality. $PM_{10}$ particles were collected from May to November 2007 in the Yongin-Suwon bordering area. $PM_{10}$ samples were collected on quartz filters by a $PM_{10}$ high-volume air sampler. The inorganic elements (Al, Mn, V, Cr, Fe, Ni, Cu, Zn, Cd, Pb, Si, Ba, Ti and Ag) were analyzed by an ICP-AES after proper pre-treatments of each sample. The ionic components of these $PM_{10}$ samples ($Cl^_$, $NO_3^-$, $SO_4^{2-}$, $Na^+$, $NH_4^+$, $K^+$, $Ca^{2+}$, and $Mg^{2+}$) were analyzed by an IC. The carbon components (OC1, OC2, OC3, OC4, OP, EC1, EC2 and EC3) were also analyzed by DRI/OGC analyzer. Source apportionment of $PM_{10}$ was performed using a positive matrix factorization (PMF) model. After performing PMF modeling, a total of 8 sources were identified and their contribution were estimated. Contributions from each emission source were as follows: 13.8% from oil combustion and industrial related source, 25.4% from soil source, 22.1% from secondary sulfate, 12.3% from secondary nitrate, 17.7% from auto emission including diesel (12.1%) and gasoline (5.6%), 3.1% from waste incineration and 5.6% from Na-rich source. This study provides information on the major sources affecting air quality in the receptor site, and therefore it will help us maintain and manage the ambient air quality in the Yongin-Suwon bordering area by establishing reliable control strategies for the related sources.

Characteristics of PM10 in Gwangju Using Factor Analysis (인자분석을 이용한 광주지역 미세먼지(PM10)의 특성 연구)

  • Lee, Se-Haeng;Seo, Gwang-yeop;Yoon, Sang-Hoon;Yang, Yoon-Cheol;Kim, Sun-Jung;Cho, Young-gwan;Bae, Seok-Jin
    • Journal of Environmental Science International
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    • v.27 no.4
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    • pp.241-250
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    • 2018
  • The objective of this study was to estimate air quality trends in the study area by surveying monthly and seasonal concentration trends. To do this, the mass concentration of $PM_{10}$ samples and the metals, ions, and total carbon in the $PM_{10}$ were analyzed. The mean concentration of $PM_{10}$ was $33.9{\mu}g/m^3$. The composition of $PM_{10}$ was 39.2% ionic species, 5.1% metallic species, and 26.6% carbonic species (EC and OC). Ionic species, especially sulfate, ammonium, and nitrate, were the most abundant in the $PM_{10}$ and had a high correlation coefficient with $PM_{10}$. Seasonal variation of $PM_{10}$ showed a similar pattern to those of ionic and metallic species. with high concentration during the winter and spring seasons. $PM_{10}$ showed high correlation with the ionic species $NO_3{^-}$ and $NH_4{^+}$. In addition, $NH_4{^+}$ was highly correlated with $SO{_4}^{2-}$ and $NO_3{^-}$. We obtained four factors through factor analysis and determined the pollution sources using the United States Environmental Protection Agency(U.S. EPA) pollution profile. The first factor accounted for 51.1% of $PM_{10}$ from complex sources, that is, soil, motor vehicles, and secondary particles: the second factor indicated marine sources; the third factor, industry-related sources; and the last factor, heating-related sources. However, the pollution profile used in this study may be somewhat different from the actual situation in Korea because it was from US EPA. Therefore, to more accurately estimate the pollutants present, it is necessary to create a pollution profile for Korea.

Tillage practices and fertilization effects on growth and nitrogen efficiency in soybean

  • Roy, Swapan Kumar;Jung, Hyun-Jin;Yoo, Jang-Hwan;Kwon, Soo Jeong;Yang, Jong-Ho;Kim, Sook-Jin;Chung, Keun-Yook;Kim, Hong-Sig;Woo, Sun-Hee
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.356-356
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    • 2017
  • A field experiment was performed to evaluate the effects of tillage systems and fertilizer management on yield and nutrient uptake in Soybean. The plant height, fresh weight and dry weight of conventional tillage were much higher those observed for no-tillage. Significant differences in plant height were observed under tillage practices combined with fertilizer treatment. However, the greatest plant height (128.47 cm) was observed in conventional tillage with chemical fertilizer, and the lowest (45.4 cm) was observed in the no-tillage with green manure treatment. The highest fresh weight (172.4 g) and dry weight (44.1 g) were observed from the no-tillage chemical treatment in the late flowering stage of soybean. The plant concentration of nitrate was higher (2.29%) in no-tillage with green manure than it was with chemical fertilization. However, nitrogen increased steadily in all treatments, and the highest quantity of total nitrogen (476.7 Kg/ha) was observed in no-tillage with green manure. The N content in the soil decreased gradually just after the vegetative stage. Tillage practices and additional fertilizer application had an adverse effect on the uptake of N, P and K in soybean seeds. The nitrogen concentration in seeds was found to be increased in the no-tillage with green manure treatment. The uptake of more nitrogen induced a better yield. Thus, the no-tillage with green manure treatment had the greatest yield, although no significant difference was observed among foliar-applications and additional fertilization. Additionally, the phosphorus and potassium concentrations in seeds remained the same between the conventional tillage and no-tillage treatments. The results obtained in this study indicate that no-tillage strategies with fertilizers may influence the growth characteristics and mineral uptake in soybean.

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Effect of Wood vinegar on Tomato Seedling Growth and Nutrient Uptake (토마토 유묘생육 및 양분흡수에 관한 목초액의 영향)

  • 김승환;최두희;이상민;남재작;김한명;손석용;송범헌
    • Korean Journal of Organic Agriculture
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    • v.11 no.2
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    • pp.103-113
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    • 2003
  • The chemical properties of oak tree wood vinegar and the effect of wood vinegar on the tomato seedling were investigated to apply wood vinegar efficiently to the organic - and natural farming system. On the basis of the results from chemical properties of the oak tree wood vinegar, mineral nutrient contents of wood vinegar was low. Therefore, wood vinegar could not be a suitable nutrient source for the plant growth at 500∼1000 times dilution level. which commonly used in the farming, if only wood vinegar is supplied for the nutrient source for the plant growth. The application of wood vinegar increased root growth up the 500 times dilution level while decreased shoot growth. Furthermore. the anion concentrations such as nitrate and phosphate of the plant were decreased by the application of wood vinegar while cation concentrations such as K. Ca. and Mg were increased. Phenolic compounds of wood vinegar such as chlorogenic acid and ferulic acid enhanced the root growth. Interestingly the application of ferulic acid increased both root and shoot growth at the level of 10$^{-4}$ M concentration. It indicated that the effect of wood vinegar on the production of healthy plant seedling may be due to the beneficial root growth by phenolic compounds such as chlorogenic acid and/or ferulic acid of the wood vinegar. However. the effect of the wood vinegar on the plant growth could be influenced by synergism or antagonism of different phenolic compounds in wood vinegar used. In addition. drench in the soil of wood vinegar may be more beneficial compared to foliar application for the improvement of root activity and plant growth.

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Isolation of Nematophagous Fungi against Root-knot Nematode and Their Growth in Vitro (뿌리혹 선충에 대한 기생 천적 진균 분리 및 이들의 생장에 미치는 환경조사)

  • 정미정;김희규
    • Korean journal of applied entomology
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    • v.27 no.3
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    • pp.149-158
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    • 1988
  • Nematophagous fungi were successfully isolated by baited plating, centrifugation technique of soil, and direct isolation from naturally ingested nematodes. Predominant seven fungi isolated were identified as Artheobotrys arthroboteyides, A.conoides, A. oligospora, Dactylella lobata, Fusatium oxysporum, Monacrosporium ellopsoporum and Harposporium anguillu-lae. Of these, six fungi were tested for cultural characteristics except. H, anguillulae, extre-mely fastidious fungus in artificial media. Among 14 media tested in this experiment, Corn-meal Agar (CMA) and Oatmeal Agar (OMA) were the most suitable media for growing all six nematophagous fungi. Weakly saprophytic M. ellipsospoyum also grew vigoroualy on these two media. The radial growth, dry weight and sporulation of the fungi tested were quite diverse depending on the culture media. D. lobata revealed good growth and abundantly sporulated on Glucoes Peptone Agar (GPA). Although over-all growth of F, oxysporum was not satisfactory on Sucrose Nitrate Agar (SNA), the sporulation was best on this medium. Optimum conditious for mycelial growth and sporulation of nematophagous fungi ranged pH 5-8 and 20-$30^{\circ}C$ on SNA. D. lobata and F, oxysporum grew vigorously and most profusely sporulated on all media tested. They turned out an most promising biocontrol agents for their aggressive growth and sporulation over the ranges of temperature and pH ranges.

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Radon Measurement by Alpha Particle Track Method (Alpha 입자비적법(粒子飛跡法)에 의(依)한 Radon 측정(測定))

  • Kang, Yung-Ho;Park, Jang-Sick;Kim, Do-Sung
    • Journal of Radiation Protection and Research
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    • v.7 no.1
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    • pp.17-22
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    • 1982
  • Measurement of the radon concentration close to the ground surface can be used in search of environmental radiation for human safety, exploration for uranium, premonitory signals from earthquakes. We can detect radons in soil gas by alpha particle track method using the plastic track detectors, cellulose nitrate (LR115-Type 2 and CA80-15, Kodak $Path\'{e}$) and CR-39. For present works, radon cups having these detectors were made in our laboratory and their conversion factor was determined. A typical conversion factor was $1tr/cm^2{\cdot}30days=1.2{\times}10^{-2}pCi/l$. In the radon cups, some of $CaSO_4$ were used as desiccant for reducing the moisture effects on plastic track detectors. With these radon cups, underground radon concentrations of Kyungpook area were measured. Average radon concentration in Daegu from Jan. 1981 to Feb. 1982 was 39.7pCi/l. From Aug. 1981 to Feb. 1982, average radon concentrations of Daegu, Angang, Kyungju, Pohang, Chungha, and Andong were 31.8pCi/l, 124.5pCi/l, 127.0pCi/l, 79.1pCi/l, 144.4pCi/l, and 70.9pCi/l, respectively. The results were compared with the environmental radiation measured by TLD method.

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Characteristics of PM2.5 in Gwangju Evaluated by Factor Analysis (인자분석을 이용한 광주지역 초미세먼지(PM2.5)의 특성 연구)

  • Lee, Se-Haeng;Lee, Kyung-Seog;Yoon, Sang-Hoon;Yang, Yoon-Cheol;Park, Ji-Young;Bae, Seok-Jin;Lee, Dae-Haeng
    • Journal of Environmental Science International
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    • v.28 no.4
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    • pp.413-422
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    • 2019
  • The objective of this study was to estimate the trends of air quality in the study area by analyzing monthly and seasonal concentration trends obtained from sampled data. To this aim, the mass concentrations of $PM_{2.5}$ in the air were analyzed, as well as those of metals, ions, and total carbon within the $PM_{2.5}$. The mean concentration of $PM_{2.5}$ was $22.7{\mu}g/m^3$. The mass composition of $PM_{2.5}$ was as follows: 31.1% of ionic species, 2.2% of metallic species, and 26.7% of carbonic species (EC and OC). Ionic species, especially sulfate, ammonium, and nitrate, were the most abundant in the $PM_{2.5}$ and exhibited a high correlation coefficient with the mass concentration of $PM_{2.5}$. Seasonal variations of $PM_{2.5}$ showed a similar pattern to those of ionic and metallic species, with high concentrations during winter and spring. $PM_{2.5}$ also had a high correlation with the ionic species $NO_3{^-}$ and $NH_4{^+}$. In addition, $NH_4{^+}$ was highly correlated with $NO_3{^-}$. Through factor analysis, we identified four controlling factors, and determined the pollution sources using the United States Environmental Protection Agency(U.S. EPA) pollution profile. The first factor, accounting for 19.1% of $PM_{2.5}$ was attributed to motor vehicles and heating-related sources: the second factor indicated industry-related sources and secondary particles, and the other factors indicated soil, industry-related and marine sources. However, the pollution profile used in this study may be somewhat different from the actual situation in Korea, since it was obtained from US EPA. Therefore, to more accurately estimate the pollutants present in the air, a pollution profile for Korea should be produced.

Introduction, Development, and Characterization of Supernodulating Soybean Mutant. 1. Mutagenesis of Soybean and Selection of Supernodulating Soybean Mutant (다량 뿌리혹 형성 콩 계통의 도입 개발 및 생육특성구명 1. 돌연변이유기에 의한 콩 초다뿌리혹형성 계통선발)

  • Lee, Hong-Suk;Chae, Young-Am;Park, Eui-Ho;Kim, Yong-Wook;Yun, Kwang-Il;Lee, Suk-Ha
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.2
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    • pp.247-253
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    • 1997
  • Development of soybean cultivars with great nodulation and high nitrogen fixation activity, derived mostly from mutagenesis, may decrease inputs of chemical fertilizer nitrogen into the soil-plant system. Soybean seeds (cv. Jangyupkong, Hwanggeumkong, and Geomjungkong 1) were treated with three different levels of EMS (ethyl methanesulfonate) concentration(30, 50, and 70mM). Increasing the doses of EMS resulted in decreased field emergence rate of seeds, whereas it did not increase M$_2$ mutation frequencies. This indicated that the most efficient concentration of EMS was 30mM for generating mutants. Extensive mutagenesis of Sinpaldalkong 2 with 30mM EMS was undertaken to isolate soybean mutants with greater nodulation. Approximately 8, 200 M$_2$ families were screened for greater nodulation on 5 mM nitrate after inoculation with Bradyrhizobium japonicum strain YCK213-KFCC-10728. Mutant SS-2 nodulated more than the wild type. Comparison of supernodulation between SS-2 and two nts mutants(nts 1007 and nts 1116) revealed that SS-2 showed the supernodulation character at an earlier growth stage than the two nts mutants. Further studies should be needed to characterize the difference in timing of nodulation between SS-2 and nts mutants.

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