• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2005년도 춘계 학술발표회
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• pp.166-166
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• 2005

• Molecular & Cellular Toxicology
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• 제2권1호
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• pp.11-14
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• 2006

In this study we attempted to develop a novel genomic method to selectively isolate target functional microbial genomes from environmental samples. For this purpose, stable isotope probing (SIP) was applied in selectively isolating organic pollutant-assimilating populations. When soil microbes were fed with $^{13}C-labeled $ biphenyl, biphenyl-utilizing cells were incorporated with the heavy carbon isotope. The heavy DNA portion was successfully separated by CsCl equilibrium density gradient. And the diversity in the heavy DNA was sufficiently reduced, being suitable for the current DNA microarray techniques to detect biphenyl-utilizing populations in the soil. In addition, we proposed a new way to get more genetic information by combining this SIP method with selective metagenomic approach. The increased selective power of these new DNA isolation methods will be expected to provide a good quality of new genetic information, which, in turn, will result in development of a variety of biomarkers that may be used in assessing ecotoxicology issues including the impacts of organic hazards, and antibiotic-resistant pathogens on human and ecological systems.

• Environmental Analysis Health and Toxicology
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• 제25권1호
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• pp.1-10
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• 2010

Monitoring toxicity levels in specific biological compartments is necessary to evaluate the ecotoxicological risk associated with soil environmental pollution. Gene expression, as potential biomarker, is increasingly used as rapid early warning systems in environmental monitoring and ecological risk assessment procedures. Various representative species are currently used for the purpose of assessing soil toxicity, however, investigations on toxicological assessments using endpoint based on gene-level have been limited. In this review, we will present the current trends in organisms and endpoints used in soil toxicity study and report gene expression related to toxicity using soil organism, and C. elegans as promising organisms for this approach.

• Environmental Analysis Health and Toxicology
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• 제23권2호
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• pp.87-91
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• 2008

In this study, three different sizes of cerium oxide ($CeO_2$) nanoparticles were synthesized and exposed to Caenorhabditis elegans to investigate the potential harmful effect of $CeO_2$ nanoparticles on the environment. The effects of the $CeO_2$ nanoparticles on C. elegans were assessed at multiple levels, such as with respect to stress response gene expression, growth, reproduction and mortality. Moreover, to test the ecotoxicological relevance of $CeO_2$-induced gene expression. The overall results suggest that $CeO_2$ nanoparticles may provoke ecotoxicity in C. elegans especially with respect to gene expression, reproduction and survival, which can comprise an important contribution to knowledge on the ecotoxicity of $CeO_2$ nanoparticles, about which little data are available. This is particularly valuable in the biomarker research on ecotoxicology, as ecological relevance is a crucial criterion for the applicability of the biomarker in field biomonitoring and ecological risk assessment.

• 농약과학회지
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• 제5권4호
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• pp.1-10
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• 2001

곤충류를 이용하여 외부로부터 유입된 물질로 인하여 변화된 환경에 적응하는 현상들을 쉽고 간단하게 평가 할 수 있다. 이와 같은 관점에서 환경변화의 영향을 측정하는 생물학적 기준으로 곤충을 이용한 환경연구의 지침들이 제안되었다. 곤충은 전 세계 동물 종 중 75%이상을 차지하고 있으며, 이러한 생물적 다양성으로 인해 외부물질에 의하여 발생한 환경변화에 독특하게 대응하는 많은 유사종들이 존재하게 되었다. 곤충은 외부물질 중 특히 농약에 의하여 생긴 환경변화의 부차적인 영향들을 조사하는데 사용되어지고 있다. 그 중에서도 톡톡이는 토양환경오염에 대한 농약의 부차적인 영향을 조사하는데 생리학적 행동학적 생물기준으로 널리 사용되어지고 있다. 본 총설에서는 농작물보호를 위해 사용된 농약들의 토양오염에 대한 부차적인 영향을 조사하기 위하여 한국에 서식하는 톡톡이를 이용하는 방안을 검토하는데 그 의의를 두었다.