• Title/Summary/Keyword: soil disinfection

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Investigation of Synthesis and Antibacterial Properties of a Magnetically Reusable Fe3O4-ACCS-Ag Nanocomposite (재사용이 가능한 나노복합재료 Fe3O4-ACCS-Ag의 제조 및 항균 특성 평가)

  • Shim, Jaehong;Kim, Hea-Won;Kim, Jin-Won;Seo, Young-Seok;Oh, Sae-Gang;Cho, Min;Park, Junghee;Oh, Byung-Taek
    • Journal of Soil and Groundwater Environment
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    • v.20 no.3
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    • pp.25-33
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    • 2015
  • In this study, Fe3O4-ACCS-Ag nanoparticles (NPs) were successfully synthesized using silica extracted from corn cob ash. The synthesized Fe3O4-ACCS-Ag NPs were characterized using X-ray diffraction (XRD), scanning electron microscopyenergy dispersive X-ray spectroscopy (SEM-EDX), transmission electron microscopy (TEM) and fourier transform infrared spectroscopy (FTIR). In addition, the potential application of Fe3O4-ACCS-Ag NPs as an antibacterial material in water disinfection was investigated using Escherichia coli ATCC 8739 as model bacteria. The antibacterial activity of synthesized composite material showed 99.9% antibacterial effect within 20 min for the tested bacteria. From this experiment, the synthesized Fe3O4-ACCS-Ag nanocomposites also hold magnetic properties and could be easily recovered from the water solution for its reuse. The reused nanocomposites presented the decreasing antibacterial efficiencies with the reuse cycle but the composite used three times still killed 90% of bacteria in 20 min.

Studies on the appearance, distribution varietal resistance and disinfection of white tip Aphelenchoides besseyi Christie of rice in Korea (청도군이서면에 발생한 수도심고병선충의 전염경로와 온탕처리방제시험)

  • Jeon-Woo Bang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.10
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    • pp.51-56
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    • 1971
  • To make certain of the white tip infection on rice, nematodes isolated from the rice seeds grown at Chongdo-goon Isu-myun and Kimje-goon Chooksan-myun were artificially inoculated and their pathway was studied. Also, studies were made for the hot water seed treatment as a measure of white tip control at different levels of water temperature and their germinablity was checked. The results are summarized as follows; 1. Causal nematodes survive in the paddy soil and cause white tip year after year. 2. Causal nematodes can survive in the rice seeds as long as the seeds loose their germinability. 3. Causal nematodes can disseminate throught the irrigation water. 4. Causal nematodes can disseminate through the soil move from a place to other place. 5. Causal nematodes can infect all of the parts of the rice and they can disseminate through any of the parts of rice. 6. The hot water treatment at 5$0^{\circ}C$ for 60 minutes were not effective controlling causal nematodes. But, either treatment of at 55$^{\circ}C$ for 15-20 minutes or at 6$0^{\circ}C$ for 10-15 minutes were effective and germination of seeds was not affected.

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An Experimental Investigation for Efficient Operation of Septic Tank (정화조의 효율적인 운영을 위한 실험적 고찰)

  • Lee, Jang-Hown;Lee, Kyeong-Soo;Kho, Soo-Hoon;Song, Min-Hee;Lee, Soo-Hyun;Lee, Yong-Hoon;Kang, Seon-Hong
    • Journal of Korean Society of Water and Wastewater
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    • v.26 no.1
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    • pp.123-129
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    • 2012
  • A septic tank is a purification treatment system where night soil and other waste matter is converted into harmless material by the activities of bacteria. Effluent from the septic tank flows into the sewer pipe, and then this effluent affects the quality of water environment and makes foul smell. In this study, through the proper maintenance of septic tank it was tried to minimize the impact of sewer pipe on water quality and fouling smell. BOD removal rate from the septic tank's effluent which exceeded legal cleaning period was investigated for the proper maintenance. BOD Removal rate of the twelve septic tank's effluent is -62.5% to 43.9%. According to the result of BOD removal rate, septic tank cleaning should be done at least once a year. And the pathogenic coliform bacillus in the twelve septic tank's effluent is average 768,000 (MPN/$100m{\ell}$). The chlorine disinfection is needed to remove the pathogenic coliform bacillus in septic tank effluent.

Diagnosis of the growth status and actual condition of the remaining old trees in the village - Focused on Hyeongok-myeon in Gyeongju-si - (마을 내 잔존 노거수의 생육현황 및 실태진단 - 경주시 현곡면을 중심으로 -)

  • Kim, Young-Hun;Deng, Bei-Jia;Chen, Geng;You, Ju-Han
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.23 no.6
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    • pp.109-123
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    • 2020
  • The purpose of this study is to provide basic data for the establishment of future countermeasures by diagnosing the growth status and actual condition of undesignated old trees distributed in Hyeongok-myeon, Gyeongju, Gyeongsangbuk-do. The number of old trees surveyed was 2 weeks in Gajeong-ri, 2 weeks in Haguri, 3 weeks in Nae-Tae-ri, 1 week in Nawon-ri, 6 weeks in Oryu-ri, 3 weeks in Sangguri, and 2 weeks in Sohyeon-ri, The trees species composition was 6 trees Celtis sinensis Pers., 1 Diospyros lotus L. trees, 4 trees Salix chaenomeloides Kimura trees, 2 Styphnolobium japonicum L. trees, and 7 Zelkova serrata (Thunb.) Makino trees. Growth status is 7.1~22.0m in height, 14.6~25.1m in long axis, 10.2~19.2m in short axis, root diameter is 76.0~236.4cm, diamter at breast height is 67.0~220.0cm, soil acidity is pH4.9~7.0, soil The hardness was measured to be 4.0-27.0mm. The result grade of the scoring data of health information is represented by monitoring generally, monitoring critically, and absolute monitoring, and it was confirmed that out of the 20 trees population in Hyeongok-myeon, the general monitoring grade was 7 weeks, the major monitoring grade was 13 weeks, and there was no absolute monitoring grade. Accordingly, the number of old trees of the general surveillance level was maintained at the current level, and ecological surgical operations were introduced for the major surveillance level, but the case of village forests should be different, and sequential treatments were the old tree urgently needed. The level and bark of the target tree, the state of the crown, the root exposure, the presence of pests and pests, the vitality and the ground condition, the degree of pollution are normal, poor, or very poor, operation and protection management, soil improvement, removal of cover, and disinfection were urgently needed for the old trees with the surveyed data. In order to compensate for these matters, continuous monitoring and management measures for the old number should be sought.

Bacteriocidal Effects of Ultraviolet Irradiation for Reducing Bovine Mastitis Derived from Environmental Contamination (우분뇨 유래 젖소 유방염 저감을 위한 자외선 조사 살균의 효과 규명)

  • Kim, Dong-Hyeok;Lim, Jung-Ju;Lee, Jin-Ju;Jang, Hong-Hee;Jang, Dong-Il;Lee, Seung-Joo;Lee, Hu-Jang;Min, Won-Gi;Kwon, Sun-Hong;Kim, Sang-Hun;Oh, Kwon-Young;Kim, Suk
    • Korean Journal of Environmental Agriculture
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    • v.27 no.4
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    • pp.435-440
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    • 2008
  • Bovine mastitis is an important disease causing serious economic loss in dairy production and food poison in public health. The major causative agents of bovine mastitis include Escherichia coli (E. coli), Streptococcus agalactiae (S. agalactiae), Staphylococcus aureus (S. aureus). These bacteria were found in milk and environmental condition such as feces, water, soil and so on. Recently, many cases of mastitis are derived from environmental contamination of micro-organisms, which important factors for the spread of this disease in farm. Ultraviolet irradiation (UV) has been used as disinfection for waste and water in clinical and industrial facilities. Moreover the UV irradiation has been used as useful bactericidal agents to remove bacterial biofilms in environmental condition. In this study, we determined the bacterial replication in different percentage of water content (PWC) in sterilized saw dust and feces complexes from farm, and results showed that slightly decreased growth pattern of E. coli and S. agalactiae but increased growth pattern of S. aureus in various PWC (200, 400 and 600%) until 144 h incubation. In the bacteriocidal effect of UV irradiation to bacteria in saw dust and feces complex, the results showed that bacteriocidal effect was depended on the UV irradiation time, irradiation distance and PWC. Especially the antibacterial activity of UV irratiation is stronger in low PWC (50%), long time irradiation (50 sec), and short distance (5 cm) than other condition of this study. Furthermore UV irradiation with stirring showed increased the bactericidal effect compared without stirring. These results suggested that bovine mastitis causing agents may survive long time in environmental condition especially saw dust and feces complexes in farm and can cause a various disease including mastitis. Moreover, these data can be used as basis for application and development of UV disinfection to control of bovine mastitis from environmental contaminated bacteria in dairy farm.

Study on the characterization of dissolved organic matters in Nakdong River (낙동강의 용존 유기물질 특성에 관한 연구)

  • Heo, Seong-Nam;Park, Jeong-Min;Im, Tae-Hyo;Shin, Chan-Ki
    • Journal of environmental and Sanitary engineering
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    • v.22 no.3
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    • pp.13-26
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    • 2007
  • Changes in the characteristics of dissolved organic matter was studied at selected stations in the Nakdong river basin using physical and chemical methods. Characteristics of dissolved organic matters were analysed and assessed. Production of disinfection byproducts were also investigated. 1. Result of relatedness study among each items of analyses showed that relatedness on BOD values were highest with cattle excrement treatment facilities where there is no synthetic organic materials and relatedness on $COD_{Mn}$ were highest at the mainstream Nakdong river. In case of $COD_{Cr}$ (which has more oxidative power than $COD_{Mn}$), the values were higher in the sidestreams indicating the higher content of recalcitrant compounds. The relatedness values for the $UV_{254}$ also showed higher values in the sidestreams and treatment facilities than mainstream indicating the presence of organic aromatic compounds. 2. Ratio of DOC on total organic carbon were higher in the mainstream which is attributable to the influent particulate organic materials produced by agricultural activities. The values were 10-15% higher in the mainstream compared with sidestreams. 3. Result of biodegradability test indicate that concentrations of recalcitrant DOC were higher in the sidestreams than in the mainstream. The values of recalcitrant DOC were higher with the forest stream indicating the effect of soil oriented humic substances. 4. Result of THM production test carried out at 10 stations in the Nakdong river show that $CHCl_3$ was detected with the highest value and the value was highest at Waekwan station.

A Study on the Pollution Sources of Simple water Supply Piped System using Statistical Analysis (통계적 분석을 이용한 간이급수시설의 오염원에 관한 연구)

  • 이홍근;김현용;백도현;김지영;이태호
    • Journal of environmental and Sanitary engineering
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    • v.14 no.2
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    • pp.56-67
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    • 1999
  • This study was performed to suggest the basic data and plans for the establishment of safe water supply plans in simple water supply piped system in the rural areas. In 4 different places, 24 points of water sources 36 points of taps from water sources were sampled. Of the whole 60 points, 55 points were ground water and 5 points were surface water. 14 items were measured for the analysis of water quality on each samples. The measured items were analyzed again by statistical method ; cluster analysis and principle components analysis. The results of this study are as followed. 1) In water quality analysis on water sources, 4 items, bacteria, E.coli, NH3-N and Fe exceed the standard. Of 24 points, 20 points(83%) on bacteria, 1 point(4%) on NH3-N and Fe exceed the standard. 2) In water quality analysis on near and remote taps, 4 items, bacteria, E.coli, NH3-N and Fe , exceed the standard. Of 36 points, 20 points (81%) on bactria, 1 pint(3%) on NH3-N and Fe exceed the standard. 3)Cluster analysis on water quality shows the differences by the kinds of water sources, geographical characteristics and distance from water sources. 4) Principle components analysis on ground water shows that Factor 1 and Factor 3 are natural fluctuation by the content of soil. Also, Factor 2 and Factor 4 are penetration of pollutants to underground. Therefore, it is needed to take deeper ground water in order to prevent from pollution in the areas which have ground water as water source . 5) Principle components analysis on surface water shows that Factor 1 is penetration of vacteria from surface to water source when rainfalls. Also, Factor 2 is fluctuation of water quality by the geographical characteristics. Therefore, the counterplans against non-point pollution source must be taken. Filtration and disinfection facilities are needed in the areas which have surface water as water source.

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Disinfection of Seed Borne Black Leg Disease(Phoma wasabiae) in Wasabi(Wasabia japonica Matsum.) (고추냉이 먹들이병(Phoma wasabiae)의 종자소독 효과)

  • Moon, Jung-Seob;Kim, Hyung-Moo;Choi, Dong-Chil;Hong, Yoon-Ki;Sung, Moon-Ho;Jang, Young-Jik;Go, Bok-Rae;Oh, Nam-Ki;Choi, Yeong-Geun
    • Journal of Bio-Environment Control
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    • v.12 no.4
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    • pp.180-183
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    • 2003
  • P. wasabiae was isolated from discolored seeds of wasabi(Wasabia japonical Mtsum.) and inoculated to fresh seeds, then the effect of fungicides on suppression of diseases were determined. Emergence rate of wasabi seeds where suppressed to 52.5% by the inoculation and it reached up to 92.7% by dipping treatment of inoculated seeds ito benomyl solution. The incidence rate of black leg disease and damping off were 32.0 and 22.0%, respectively, in control treatment that sown in the soil inoculated with P. wasabiae. But dipping treatment of inoculated seeds into benomyl solution resulted in 12.0% and 10.7% in incidence rate of those two diseases, respectively.

Current Studies on Bakanae Disease in Rice: Host Range, Molecular Identification, and Disease Management

  • Yu Na An;Chandrasekaran Murugesan;Hyowon Choi;Ki Deok Kim;Se-Chul Chun
    • Mycobiology
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    • v.51 no.4
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    • pp.195-209
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    • 2023
  • The seed borne disease such as bakanae is difficult to control. Crop yield loss caused by bakanae depending on the regions and varieties grown, ranging from 3.0% to 95.4%. Bakanae is an important disease of rice worldwide and the pathogen was identified as Fusarium fujikuroi Nirenberg (teleomorph: Gibberella fujikuroi Sawada). Currently, four Fusaria (F. fujikuroi, F. proliferatum, F. verticillioides and F. andiyazi) belonging to F. fujikuroi species complex are generally known as the pathogens of bakanae. The infection occurs through both seed and soil-borne transmission. When infection occurs during the heading stage, rice seeds become contaminated. Molecular detection of pathogens of bakanae is important because identification based on morphological and biological characters could lead to incorrect species designation and time-consuming. Seed disinfection has been studied for a long time in Korea for the management of the bakanae disease of rice. As seed disinfectants have been studied to control bakanae, resistance studies to chemicals have been also conducted. Presently biological control and resistant varieties are not widely used. The detection of this pathogen is critical for seed certification and for preventing field infections. In South Korea, bakanae is designated as a regulated pathogen. To provide highly qualified rice seeds to farms, Korea Seed & Variety Service (KSVS) has been producing and distributing certified rice seeds for producing healthy rice in fields. Therefore, the objective of the study is to summarize the recent progress in molecular identification, fungicide resistance, and the management strategy of bakanae.

Production of Organic Rice (Oryza sativa L.) using Organic Cultivation Manual (유기재배매뉴얼을 활용한 유기쌀 생산)

  • Cha, Kwang-Hong;Oh, Hwan-Jung;Seo, Dong-Jun;Song, Yong-Su;Ahn, Joon-Seob;An, Kyu-Nam;Jung, Woo-Jin
    • Korean Journal of Organic Agriculture
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    • v.22 no.1
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    • pp.97-113
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    • 2014
  • An actual proof experiment was carried out to establish the production of high quality organic rice at farm household in Gwangjin, Junnam, Korea 2011. Experimental plot was designed with conventional organic cultivation and manual organic cultivation in both Hopeongbyeo and Onnuri varities. Experimental field was consisted with suitable drainage loam soil, anryong series, and normal paddy field. Chemical characteristics of the soil were investigated from initial soil and soil after harvest in experimental sites. Seed disinfection was treated with hot water dipping method at $60^{\circ}C$ for 10 min. Rice transplanting was carried out at 17 June with 25 days seedling using machine transplanting. Golden apple snail was used to prevent weed growth as middle size (1.5~1.7 kg/10a) at one week after rice transplanting. Amount of applied fertilizer in experimental plot was supplied with organic fertilizer for the shortage after harvest of hairy vetch (1,100 kg/10a). Bordeaux mixture was used to control of sheath blight and neck blast disease in all experimental plot at 19 Aug. Application of chitin culture solution was treated in manual organic cultivation at 2 and 15 Aug. At late growth stage, incidence rate of neck blast was the highest level at Hopyeongbyeo in conventional organic cultivation. Incidence rate of sheath blight in both Hopyeongbyeo and Onnuri was higher in conventional organic cultivation than in manual organic cultivation. Incidence of rice leaf folder was the highest level at Onnuri in conventional organic cultivation. Yield index in manual organic cultivation was increased by 13% and 38% at Onnuri and Hopyeongbyeo, respectively, compared with conventional organic cultivation. Head rice percentage in both Hopyeongbyeo and Onnuri was slightly increased in manual organic cultivation. These results indicate that high quality production of rice organic cultivation could be depending on management of safe fertilization with nutrient content in soil.