• 한국유기농업학회지
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• 제26권4호
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• pp.775-787
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• 2018

본 연구는 반추동물의 메탄 감소에 효과가 있는 합성첨가제를 대체 할 수 있는 천연첨가제를 찾고자 연구를 실시하였다. 첨가제인 MRA-1(파), MRA-2 (SLS), MRA-3 (SDS), MRA-4(파+탄닌산)를 만들어 발효시간대별(3, 6, 9, 12, 24 및 48 h) in vitro 시험을 실시하였다. pH는 발효시간동안 적정범위였으며, 건물소화율은 모든 발효시간대별 모든 처리구에서 대조구와 유의적(P>0.05)인 차이를 보이지 않았고, 미생물성장량에도 부정적인 영향을 미치지 않았다. 메탄 발생량은 발효 24시간대 MRA-1구를 제외한 나머지 처리구에서 대조구에 비해 유의적(P<0.05)으로 낮게 측정되었으며, 특히 이산화탄소 발생량은 MRA-4 첨가구에서 발효 9, 24, 48시간대에서 유의적(P<0.05)으로 낮았다. 메탄생성량 감소에 영향이 있는 프로피온산 함량은 발효 24시간대 MRA-4구에서 대조구에 비해 유의적(P<0.05)으로 높았다. Ciliate-associated methanogens은 발효 24시간대 MRA-1, MRA-3 및 MRA-4구에서 대조구에 비해 유의적(P<0.05)으로 낮게 측정되었다. 따라서 파와 탄닌산은 반추위 내 메탄 저감 효과가 있는 합성첨가제를 대체 할 수 있어 반추동물용 천연 메탄 저감제로서의 활용 가능성이 있을 것으로 사료된다.

• 대한한방소아과학회지
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• 제32권3호
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• pp.90-99
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• 2018

Objectives Hwangnyeonhaedok-tang is a Korean herbal medical treatment that removes toxic heat, fever and inflammation. The purpose of this study was to investigate the effect of Hwangnyeonhaedok-tang treatment on the relief of atopic dermatitis (AD) through regeneration of skin lipid barrier. Methods Male NC/Nga mice (20 g, 6 week age) were used. Each 10 mice were allocated to the control group (Ctrl), the AD-induced with no treatment group (AE), and the group which induced AD after administering Hwangnyeonhaedok-tang extract (HT). To induce AD-like skin lesions, sodium dodecyl sulfate (SDS) (Sigma-Aldrich, USA) was rubbed on the back of each mouse to remove the lipid lamella of the stratum corneum, and Dermatophagoides (D.) farinae crude extract was applied. HT group was orally administered Hwangnyeonhaedok-tang after induction of AD. IL-4 IL-13, $p-I{\kappa}B$, iNOS, Sudan Black B (SB), loricrin, and filaggrin were observed to confirm the effect. Results In HT group, AD skin score was decreased by 46%. The cytokine IL-4 and IL-13, which can identify Th2 differentiation, was reduced by 73% and 58% each. Anti-inflammatory effects were observed in $p-I{\kappa}B$ and iNOS by 69% and 54%, respectively. Finally, SB showed that the regeneration of the lipid layer and the increase of the regeneration power of loricrin and filaggrin were increased by 437% and 464%, respectively. Conclusions From the study result, we observed that Hwangnyeonhaedok-tang treatment alleviates AD by decreasing skin score, reducing Th2 differentiation, inducing anti-inflammatory, and increasing skin lipid barrier regeneration. Thus, Hwangnyeonhaedok-tang treatment would be considered as an effective AD relieving treatment.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2007년도 춘계학술대회
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• pp.485-488
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• 2007

$SF_6$(sulfur hexafluoride)는 뛰어난 단열 및 아크방지능력(arc-extinguishingproperty)으로 인해, 전력용 변압기의 절연가스와 반도체${\cdot}$액정용 플라즈마 CVD로의 cleaning gas, 주물공장 covering gas 등으로 사용되고 있다. 하지만, $SF_6$의 지구온난화지수(global warming potential)는 $CO_2$대비 23,900배가 높아 기후변화에 미치는 영향이 $CO_2$보다 훨씬 크고, 대기 중 분해되지 않고 잔존하는 기간이 $CH_4$ 10년, $CO_2$ 및 CFCs는 100년으로 추정되는데 반해, $SF_6$는 3,200년으로 연간방출양이 작더라도 오랜 기간 누적되면 그 파장이 클 것으로 사료된다. 대부분의 가스 하이드레이트(고상결정상태)는 고압, 저온에서 형성가능 하지만, 불화가스에 대해서는 쉽게 결정화가 일어난다. $SF_6$는 3$^{\circ}C$, 2기압에서 고밀도 고상화가 되기 때문에 여러 기체와 흔합되어 있는 경우 $SF_6$만을 압축된 고상 결정상태를 형성, $SF_6$를 회수, 정제할 수 있으므로 불화가스 분리${\cdot}$회수에 기술적, 경제적 효과를 기대할 수 있다. 본 연구에서는 하이드레이트 촉진제로서 계면활성제(promoter) 첨가에 따른 $SF_6$ 하이드레이트 형성 및 해리과정 실험을 통해 효율적인 $SF_6$ 저감에 관한 적용기술을 연구해 보았다.

• Journal of Animal Science and Technology
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• 제49권1호
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• pp.129-136
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• 2007

본 실험은 한우 육단백질의 가수분해물로부터 항고혈압 활성을 측정하기 위하여 실시한 것으로서 한우 등심과 우둔으로부터 추출한 myosin B를 pepsin으로 가수분해하여 가수분해물들의 전기영동 결과, 가열처리와 가수분해 시간의 증가에 따라 단백질의 소실이 증가하였다. 항 고혈압 활성을 측정한 결과 10 ug/ml의 희석된 가수분해물의 ACE 억제효과는 1시간 이상 가수분해 시키면 약 40%의 억제율을 가졌다. 가수분해물 원액으로 ACE 억제효과를 살펴본 결과에서는 등심이 우둔보다 높았으며 (p<0.05), 비가열 가수분해물이 가열한 가수분해물 보다 억제율이 높게 나타났다 (p<0.05). 또한, 가수분해 시간별 처리구에서는 1시간 이상 가수분해 시키면 약 70% 이상의 억제율을 갖는 것으로 나타나 한우의 myosin B를 1시간 이상 가수분해하면 ACE 억제율이 증진되는 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제11권2호
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• pp.317-325
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• 2001

For heuristic purposes, the relative ratio of urease contents inside and outside cells was surveyed using nine ureB+ strains of Helicobacter pylori. the ratio of the enzyme specific activity appeared to vary greatly between the various H. pylori strains, ranging from 0.5 to 2.5. Besides the above compartment, urease was also richly found in the membrane fraction, especially in either peripheral or integral form. The urease distribution across the H. pylori membrane was significantly influenced by the ambient pH; the specific activity of external urease was highest at pH 5.5 with a narrow plateau, whereas the internal specific activity was highest within a pH range of 4.5 to 6.5 with a broad plateau. These finding strongly suggest that H. pylori urease is secretory and responded to the external pH. However, at pH 4.0 or below, no urease activity was detected in either the internal or external compartment, although an increase in the color development with 2,4,6-trinitrobenzene sulfonate (TNBS) was observed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that these phenomena may be related to a specific proteolysis in certain proteins, including urease or ${\gamma}$-glutamyl transpeptidase. Interestingly, the effect of ammonium ions n alleviating the enzyme inactivation inside the H. pylori cells was remarkably similar to that of D-glucose. In addition, it would appear that the cation acted as a surrogate of not only $Na^+$ but also $K^+$ thereby increasing the H. pylori P-type ATPase activity. This is of great interest, as it implies that the urease action in H. pylori is indispensible at any locus.

• Applied Biological Chemistry
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• 제36권3호
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• pp.158-162
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• 1993

토양에서 분리한 bilirubin oxidase를 생산하는 Penicillium sp. LAM 91-89의 배양액으로부터 효소를 정제하여 여러가지 성질을 조사하였다. LAM 91-89 billirubin oxidase는 $ethanol(40{\sim}80%)$ 추출 및 2회의 Sephadex G-200 column chromatography로 약 70배 정제하였으며 회수율은 12%였다. 이 효소는 분자량이 약 53,000 dalton이었으며 $40^{\circ}C$, pH 8.5에서 최대의 활성을 보였고 pH 6에서 10까지의 넓은 범위와 $40^{\circ}C$ 이하에서 안정하였다. 또 효소의 활성이 $Mg^{2+}$에 의해서 증가하였으며 $Ag^+,\;Hg^{2+},\;Mn^{2+},\;Pb^{2+}$, p-CMB, iodoacetate 및 SDS에 의해서 저해되었다. 그리고 이 효소는 bilirubin에 대한 특이성이 컸으며 bilirubin에 대한 $K_m$값은 $6.67{\mu}M$이였다.

• Current Research on Agriculture and Life Sciences
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• 제8권
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• pp.9-18
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• 1990

1. 잡초종 54종을 수집 보관중이며 이들을 대상으로 발아력을 검정한 결과 치상후 15일째에 31종이 발아력을 보이고 그 가운데 50% 이상의 발아력을 보인것은 14종에 불과하여 대부분의 잡초종은 휴면의 정도에는 차이가 있지만 어떤 형태로든지 휴면성을 갖고 있는 것으로 구명되었다. 2. 우리나라에 발생하는 피속 잡초종은 외형적으로 여러종이 발생하는것 같았으나 2종만이 존재하는데 한 종은 강피이고 다른 종은 돌피와 변종으로 물피가 존재하는 것으로 밝혀졌다. 3. 종자의 단백질 패턴 검정으로 종자 형태학적으로 분류한 종들을 재점검해 본 결과 강피는 돌피와 물피와는 달리 B zone에 주요 band가 있어서 쉽게 구분되었으나 돌피와 물피의 단백질 패턴이 유사하여 구분이 쉽지 않았다.

• Journal of Microbiology and Biotechnology
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• 제12권1호
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• pp.39-45
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• 2002

Activity staining on the native polyacrylamide gel electrophoresis (PAGE) of a cell-free extract of Rhodococcus sp. TK6, grown in media containing alcohols as the carbon source, revealed at least seven isozyme bands, which were identified as alcohol dehydrogenases that oxidize cyclohexanol to cyclohexanone. Among the alcohol dehydrogenases, cyclohexanol dehydrogenase II (CDH II), which is the major enzyme involved in the oxidation of cyclohexanol, was purified to homogeneity. The molecular mass of the CDH II was determined to be 60 kDa by gel filtration, while the molecular mass of each subunit was estimated to be 28 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The CDH II was unstable in acidic and basic pHs, and rapidly inactivated at temperatures above $40^{\circ}C$ . The CDH II activity was enhanced by the addition of divalent metal ions, like $Ba^2+\;and\;Mg^{2+}$. The purified enzyme catalyzed the oxidation of a broad range of alcohols, including cyclohexanol, trans-cyclohexane-1,2-diol, trans-cyclopentane-l,2-diol, cyclopentanol, and hexane-1,2-diol. The $K_m$ values of the CDH II for cyclohexanol, trans-cyclohexane-l,2-diol, cyclopentanol, trans-cyclopentane-l,2-diol, and hexane-l,2-diol were 1.7, 2.8, 14.2, 13.7, and 13.5 mM, respectively. The CDH II would appear to be a major alcohol dehydrogenase for the oxidation of cyclohexanol. The N-terminal sequence of the CDH II was determined to be TVAHVTGAARGIGRA. Furthermore, based on a comparison of the determined sequence with other short chain alcohol dehydrogenases, the purified CDH II was suggested to be a new enzyme.

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1557-1567
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• 2012

Glutathione reductase (GR, E.C. 1.6.4.2) is an important enzyme that reduces glutathione disulfide (GSSG) to a sulfydryl form (GSH) in the presence of an NADPH-dependent system. This is a critical antioxidant mechanism. Owing to the significance of GR, this enzyme has been examined in a number of animals, plants, and microbes. We performed a study to evaluate the molecular properties of GR (OsGR) from rice (Oryza sativa). To determine whether heterologous expression of OsGR can reduce the deleterious effects of unfavorable abiotic conditions, we constructed a transgenic Saccharomyces cerevisiae strain expressing the GR gene cloned into the yeast expression vector p426GPD. OsGR expression was confirmed by a semiquantitative reverse transcriptase polymerase chain reaction (semiquantitative RT-PCR) assay, Western-blotting, and a test for enzyme activity. OsGR expression increased the ability of the yeast cells to adapt and recover from $H_2O_2$-induced oxidative stress and various stimuli including heat shock and exposure to menadione, heavy metals (iron, zinc, copper, and cadmium), sodium dodecyl sulfate (SDS), ethanol, and sulfuric acid. However, augmented OsGR expression did not affect the yeast fermentation capacity owing to reduction of OsGR by multiple factors produced during the fermentation process. These results suggest that ectopic OsGR expression conferred acquired tolerance by improving cellular homeostasis and resistance against different stresses in the genetically modified yeast strain, but did not affect fermentation ability.

• Preventive Nutrition and Food Science
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• 제7권4호
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• pp.373-377
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• 2002

Allergens in processed foods may place persons with food allergies at significant risk when the labels do not Provide sufficient warnings or identification of high-risk ingredients. Because egg proteins are common food allergens, this study was carried out to identify hen's egg albumin (ovalbumin, OVA) in five commercially processed foods containing egg (custayd, cookie and pasta), and chicken meat (sausage and meatball) by immunological methods using commercially produced murine monoclonal immunoglobulin G (M-IgG), immunoblotting and enzyme linked immunosorbent assay (ELISA). Sample buffer with chelating and reducing agents was prepared and used for the preparation of the protein fractions from the foods. Most bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) profile (5~15% gradient gel) presented at 75 kDa below. OVA (43 kDa) in the sample lanes could not be visually observed on the gel. However, OVA in solutions prepared from custard and cookie could be detected by M-IgG, but were not detected in sausage and pasta. OVA in all samples could be quantitatively determined by the equation obtained from the standard curve by ELISA. Cookie and custard containing egg white and egg, respectively, contained very high concentrations of OVA. OVA in the other products were present in relatively low concentrations, but sufficiently high to pose possible risk of allergy, ELISA is a very sensitive and precise method for the identification and quantification of allergens in food products including allergy-inducible materials.