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http://dx.doi.org/10.5187/JAST.2007.49.1.129

Evaluation of Angiotensin -I- Converting Enzyme Inhibitory Activity and Protein Changes of Enzymatic Hydrolysate Extracted from Hanwoo Loin and Round Myosin B  

Kim, Y.J. (Dept. of Animal Science and Institute of Agricultural Science and Technology, Chonnam National University)
Chin, Koo-Bok (Dept. of Animal Science and Institute of Agricultural Science and Technology, Chonnam National University)
Publication Information
Journal of Animal Science and Technology / v.49, no.1, 2007 , pp. 129-136 More about this Journal
Abstract
This study was performed to determine the protein profiles using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Angiotensin-I-converting enzyme(ACE) inhibitory activity (IC50) as affected by the various meat cuts, digestion times with pepsin. Hydrolysates having the protein concentration of 10 ug/mL had approximately 36∼39% ACE inhibitory activities, regardless of meat cut and digestion time. Protein concentration and ACE inhibitory activity of the diluted hydrolysate increased after 1-hr digestion. In original hydrolysates, ACE inhibitory activities of loin had higher than those of round (P<0.05). In addition, non-heated hydrolysates had higher ACE inhibitory activities than heated counterparts. When myosin B was digested by pepsin more than 1 hr, improved ACE inhibitory activities were observed as compared to the non-digested control.
Keywords
Angiotensin converting enzyme(ACE) inhibitory activity; Myosin B; Hanwoo loin and round;
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Times Cited By KSCI : 3  (Citation Analysis)
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