• Parasites, Hosts and Diseases
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• v.51 no.5
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• pp.537-544
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• 2013

The present study was performed to observe histopathological changes in tissues of Bithynia siamensis goniomphalos (Gastropoda, Bithyniidae) incubated in crude extract solutions of camellia (Camellia oleifera) seed and mangosteen (Garcinia mangostana) pericarp, and furthermore to estimate the molluscicidal effects of 2 plant substances. Substantial numbers of bithyniid snails were incubated in various concentrations of 2 plant solution for 24 hr. As the positive control, snails incubated in various concentrations of niclosamide, a chemical molluscicide, were used. The histopathological findings were observed in sectioned snail specimens of each experimental and control groups. The results showed that both camellia and mangosteen extracts had molluscicidal effects at 24 hr with 50% lethal concentration ($LC_{50}$) at concentrations of 0.003 and 0.002 g/ml, respectively, while niclosamide had $LC_{50}$ at concentrations 0.599 ppm. B. siamensis goniomphalos snail tissues (foot, gill, and digestive system) showed disruption of columnar muscle fibers of the foot, reduction of the length and number of gill cilia, numerous mucous vacuoles, and irregularly shaped of epithelial cells. Irregular apical and calciferous cells, dilatation of the digestive gland tubule, and large hemolymphatic spaces, and irregular apical surfaces, detachment of cilia, and enlargement of lysosomal vacuoles of epidermis were also shown in all groups. By the present study, it is confirmed that 2 plants, camellia and mangosteen, are keeping some substance having molluscicidal effects, and histopathological findings obtained in this study will provide some clues in further studies on their action mechanisms to use them as natural molluscicides.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.1
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• pp.45-49
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• 2011

As a first step toward identifying a new method for testing sea snail tissue for toxins, and thus prevent food poisoning due to the ingestion of contaminated snails, we measured the tetramine [$(CH_3)_4N^+$] contents of sea snails from the Korean coast using both liquid chromatography-tandem mass spectrometry (LC-MS/MS) and ion chromatography. For tetramine tested, good linearity ($r^2$ = 0.9996) was observed between the amounts in the injected samples and the peak areas of standard toxins, which ranged from 0.1 to 100 ng. The recovery (%) of tetramine from spiked tissue and mid-gut gland samples ranged from 84.0 to 95.3%. The quantitative results for tetramine using this method were in good agreement with the theoretical values. LC-MS/MS has both high sensitivity and selectivity, which makes it possible to measure trace quantities of tetramine in samples.

• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2009.12a
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• pp.284-284
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• 2009

EFA production systems have through necessity resulted in the development of innovative practices for weed, pest and diseases control, for example, using ducks and snails for weed control in paddy fields. These practices began to be introduced in the early 1990's and the techniques have become more popular and have been adapted to suit regional conditions. In this study, the production practices, productivity and economic performances of organic and non-chemical rice farming adopting ducks and snails for weed control were compared. In the production practices, Korean organic and non-chemical farming seem to have several concerns in terms of sustainability. It comprises lack of resistant variety use and rotational cropping system as well as high dependency upon external inputs such as organic fertilizer and farming materials for pest control. The production level of organic farming is approximately similar level but 20% higher income than non-chemical farming, while, when it was compared with conventional farming organic farming showed 20% lower productivity but 20% higher income. Organic farming shows 15% to 18% higher profits than non-chemical farming as the snail-using organic farming tends to have higher income and lower input costs than duck-using organic farming. This may encourage more farmers to convert to organic production using these techniques than simply non-chemical farming in the future. This organic conversion could be more promoted by policy intervention. However, it may result in increased supply and therefore decreased prices for organic rice in the long term unless further market demand occurs. Balanced policy measures considering production as well as marketing and consumption are urgently required for the sustainable development of organic farming.

• Parasites, Hosts and Diseases
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• v.26 no.3
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• pp.189-198
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• 1988

The fresh water snail, Hippeutis (Helicorbis) cantori, caught from the rice paddies in Namyangju-gun, Gyeonggi.do, was found to be a new second inter- mediate host of 5. cinetorchis in Korea. The metacercariae were found from 89 (76.7%) out of 116 snails examined and the average metacercarial burden was 4.7 per infected snail. Some developmental characteristics of 5. cinetorchis were observed after experimental infection of the metacercariae to rats and mice.

• Parasites, Hosts and Diseases
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• v.62 no.1
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• pp.85-97
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• 2024

This study aimed to describe the morphological and molecular characteristics of Paralecithodendrium longiforme (Digenea: Lecithodendriidae) adults and cercariae isolated in Thailand. Adult flukes were isolated from the Chinese pipistrelle bat (Hypsugo sp.), and cercariae were detected in the viviparid snail (Filopaludina martensi martensi) from Chiang Mai province. The morphological characteristics were observed and described using conventional methods, and the molecular characteristics with internal transcribed spacer 2 (ITS2) and 28S rDNA gene sequences. The adult flukes were fusiform, 0.84-0.98 mm in length, and 0.37-0.49 mm in width, and were distinguishable from other species by the presence of longitudinal uterine coils. The cercariae were nonvirgulate xiphidiocercariae, with the oral sucker bigger than the acetabulum, the tail without fin fold, a body size of 117.5-138.3×48.3-52.2 ㎛, and a tail size of 100.7-103.7×15.0-18.9 ㎛. Molecular studies revealed that the adults and cercariae shared 99.3% (ITS2) and 99.6% (28S rDNA) homology with each other. They were phylogenetically close to P. longiforme with an identity of 94.5% for ITS2 and 98.7% for 28S rDNA. This study provides new information on the natural definitive host and first intermediate host of P. longiforme in Thailand. The discovery of its cercarial stage in Filopaludina snails highlights the importance of monitoring the associated second intermediate host and prevention and control of this potentially zoonotic trematode.

• Journal of the Korean Applied Science and Technology
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• v.33 no.1
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• pp.143-154
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• 2016

This study is related to develop a snail extract through a snail secondary fermentation process, getting anti-aging activity with healthy and beauty skin care scientific applications. In order to obtain a primary fermentation was incubated with Hericium erinaceus mycelium. Through the secondary fermentation process using Leuconostoc mesenteroides, was deeply described a total process of obtaining second fermented extract using snail body. Mycelium is applied in this study was extracted using Hericium erinaceus mycelium and Leuconostoc mesenteroides. The final yield of the extract was 62 wt%. Experimental results of secondary fermentation snail extract were contained with 32 wt% water, 31.5 wt% total amino acid protein, 15.7 wt% polysaccharide, 12.3 wt% fatty acid and others 8.5 wt%. In addition, in order to study about skin beauty care and anti-aging activity, we evaluated antioxidant activity with DPPH, elastin enzyme (elastase) inhibitory activity, tyrosinase inhibition rate, collagen synthetic function, fibroblast synthetic activity. First; anti-oxidative activity of secondary fermentation snail extract (IC50%) was spent with 7.27 mg/mL, control samples were spent with green tea extract was 11.8 mg/mL, common snails extract was 15.7 mg/mL, DL-a-tocopherol was 9.25 mg/mL respectively. Second; elastin enzyme inhibitory activity of secondary fermentation snail extract (IC50%) was spent with 32.5 mg/mL, control samples were also spent with green tea extract was 45.9 mg/mL, general snail extract was 67.7 mg/mL. Third; tyrosinase inhibitory activity of secondary fermentation snail extract (IC50%) was spent with 140.3 mg/mL, control samples were also spent with green tea extract was 250.7 mg/mL, general snails extract was 389.5 mg/mL, niacineamide was 125.9 mg/mL. Forth; fibroblast synthetic activity of secondary fermentation snail extract was increased with 125.6%, control samples were also spent with green tea extract was 98.9%, general snails extract was 109.5%, niacineamide was 125.9 mg/mL, DL-a-tocopherol was 96.2%. Fifth; collagen synthetic activity of secondary fermentation snail extract was increased with 118%, control samples were also spent with green tea extract was 87.3%, general snails extract was 93.2%, adenosine was 127.9%. In conclusion, on the basis of this study, in the future it is expected to be applied to the skin beauty care application and development of Korean style cosmetic products.

• Parasites, Hosts and Diseases
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• v.21 no.1
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• pp.118-124
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• 1983

The distribution, external morphology, radula, chromosome numbers of Planorbidae snails were studied. 1. The specimens were collected at four stations in Nonsangun, Kongjugun, and Daedukgun which are located around Geum river. Three genera and three species of Planorbidae, Hippeutis cantorir Segmentina hemisphaerula and Gyraulus cenvexiusculus, were collected. H. cantori was the most abundant species among the three species. G. convexiusculus was the least abundant one. 2. Each species could be identified on the basis of its external characteristic, since the periphery of each species has a peculiar shape. H. cantori was the largest one among the three species. 3. The radula formula of each species was very similar to other species. The size of radula was proportional to the size of shell. The radula formulae of H. cantori, S. hemisphaerula, and G. convexiusculus were 29 : 1 : 29, 23 : 1 : 23, and 16 : 1 : 16 respectively. The difference of radula formula could be found in the total numbers of laternal and marginal teeth. 4. The haploid chromosome number of H. cantori was eighteen (n=18), S. hemisphaerula and G. convesiusculus were assumed to be same in their chromosome numbers (n: 18).

• The Korean Journal of Malacology
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• v.10 no.2
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• pp.1-9
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• 1994

Molluscs from the Neolithic shell middens (Sugari, Pudjeong, Tongsamdong, Sangnodaedo, Sandeung, Yondaedo, Songdo and Kupyongri) in the southern coast, Korea, consisted of marine, fresh-water and land molluscs. Among these, intertidal species were exploited most abundantly as food resources, indicating a high dependence of intertidal shellfish collection activities. Especially oysters were the most abundant in these sites except Tongsamdong where mussels were predominant, indicating oysters were the most important food resources. Land snails from the sites consisted of woodland and open land species. Woodland species were more abundant than openland species, suggesting that there was a woodland environmint around the sites. Marine and land mollusc species from the sites are common in these rigions today, indicating no large-scale emvironmental changes have occurred since these middens formation period.

• Journal of Species Research
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• v.5 no.3
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• pp.530-532
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• 2016

A single specimen in the subfamily Coralliophilinae in Family Muricidae was collected from Jeju-do by SCUBA diving survey. It was identified as Hirtomurex nakamurai Kosuge, 1985 based on external morphological characteristics. The Coralliophilinae species, commonly known as coral snails, are broadly distributed in tropical and temperate oceans from shallow subtidal zones up to 1,000 m in depth. To date, only four Coralliophila species in the subfamily Coralliophilinae have been reported from Korean waters. Prior to this report, Hirtomurex species has not been reported from Korean waters yet. As a result of this study, a total of five species in two genera of subfamily Coralliophilinae are recorded as Korean mollusk fauna.

• The Korean Journal of Malacology
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• v.17 no.1
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• pp.57-61
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• 2001

The present study examines the potential involvement of Biomphalaria glabrata, a known molluscan intermediate host of Schistosoma mansoni, in the life cycle of Echinostoma cinetorchis, one of the echinostomes that are ubiquitous parasites of vertebrates and are of importance in human and veterinary medicine and wildlife diseases. Echinostomes can be maintained easily and inexpensively in the laboratory and provide good models for biological research ranging from the molecular to the organismal. In the present study, no echinostome cercariae were released from the B. glabrata experimentally infected with E. cinetorchis miracidia, whereas all the Biomphalaria snails infected with E. cinetorchis cercariae were found to be infected with the metacercariae. This is the first report that B. glabrata can experimentally serve as the second intermediate host of E. cinetorchis, and that it might be employed as one of the target molluscs for establishing a biological research model with E. cinetorchis in the laboratory.