Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Tetramine Analysis using Liquid Chromatography-Tandem Mass Spectrometry and Ion Chromatography

LC-MS/MS와 Ion Chromatography를 이용한 테트라민 분석

  • Song, Ki-Cheol (Food Safety Research Division, National Fisheries Research and Development Institute) ;
  • Lee, Ka-Jeong (Food Safety Research Division, National Fisheries Research and Development Institute) ;
  • Kim, Ji-Hoe (Food Safety Research Division, National Fisheries Research and Development Institute) ;
  • Yoon, Ho-Dong (Food Safety Research Division, National Fisheries Research and Development Institute) ;
  • Yu, Hong-Sik (Food Safety Research Division, National Fisheries Research and Development Institute) ;
  • Mok, Jong-Soo (Food Safety Research Division, National Fisheries Research and Development Institute)
  • 송기철 (국립수산과학원 식품안전과) ;
  • 이가정 (국립수산과학원 식품안전과) ;
  • 김지회 (국립수산과학원 식품안전과) ;
  • 윤호동 (국립수산과학원 식품안전과) ;
  • 유홍식 (국립수산과학원 식품안전과) ;
  • 목종수 (국립수산과학원 식품안전과)
  • Received : 2011.01.11
  • Accepted : 2011.02.10
  • Published : 2011.02.28
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Abstract

As a first step toward identifying a new method for testing sea snail tissue for toxins, and thus prevent food poisoning due to the ingestion of contaminated snails, we measured the tetramine [$(CH_3)_4N^+$] contents of sea snails from the Korean coast using both liquid chromatography-tandem mass spectrometry (LC-MS/MS) and ion chromatography. For tetramine tested, good linearity ($r^2$ = 0.9996) was observed between the amounts in the injected samples and the peak areas of standard toxins, which ranged from 0.1 to 100 ng. The recovery (%) of tetramine from spiked tissue and mid-gut gland samples ranged from 84.0 to 95.3%. The quantitative results for tetramine using this method were in good agreement with the theoretical values. LC-MS/MS has both high sensitivity and selectivity, which makes it possible to measure trace quantities of tetramine in samples.

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References

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