• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제30권3호
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• pp.186-192
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• 2004

Purpose: Human tooth proteins are highly heterogeneous, comprising diverse proteins derived from a number of genes. The attempts to identify protein for activity of tooth matrix proteins have been defied by several factors. First, the amount of proteins within teeth is very small relative to many extracellular matrix proteins of other tissues. Second, the bioassay system is tedious and needed for long time. Therefore we tried to find easy techniques, which increase the product rate, and an assay of small proteins, with which amino acid sequence is possible without additional procedures. Materials and Methods: Total protein were extracted from 300 g enamel removed teeth and 600 g teeth with 4 mol/L guanidine HCl and purified by gel chromatography. Aliquot of proteins was implanted into muscle pouches in Sprague-Dawley rats for bioassay. By SDS-PAGE and membrane blotting, molecular weight of each protein was estimated and a partial amino acid sequence was obtained. Each fraction blotted on the membrane was cut out and inserted in rat ectopic model. Results: In dissociative method, total tooth proteins were obtained 1mg/ml from enamel removed teeth and 3.5 mg/ml from teeth. In SDS-PAGE, four clear bands at the sites corresponding to 66, 40, 20 and 18 kD. Especially The 66 kD band was clearly exhibited. Amino acid sequencing from tooth could be possible using PVDF membrane blotting technique. In amino acid sequencing, 66 kD protein was identified as albumin. Conclusion: Compared with conventional method for extraction of teeth protein and bioassay of proteins, the methods in this study were easy, time-saving and more productive technique. The matured tooth proteins omitting additional procedure of mechanical removal of enamel were simply analyzed using blotted PVDF membrane. This method seems to make a contribution as a technique for bioassay and amino acid sequencing of protein.

• 한국임상수의학회지
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• 제34권6호
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• pp.429-433
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• 2017

The purpose of this study was to find anatomical variation of each small breed dog's dentition for designing more effective dental chews. Small breed dogs were volunteered for dental impressions with dental stone and alginate under tiletamine-zolazepam, tramadol, and medetomidine intravenous anesthesia. Twenty-two criteria were measured to compare dental impressions. Twenty-five dogs (9 Malteses, 8 Miniature Poodles, and 8 Shih-Tzus) were recruited. Statistically, Shih-Tzus had smaller teeth and shorter interdental spaces than those of Maltese and Miniature Poodles. Grossly, the distance between upper teeth and lower teeth was wider in Shih-Tzus. Shih-Tzu had different dental anatomy in comparative to Miniature Poodle and Maltese. It was recommended to design the dental chew considering these differences.

• 대한수의학회지
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• 제36권2호
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• pp.483-494
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• 1996

The present study was undertaken to identify the species of nematodes and determine infection state of small intestinal nematodes from dogs in Taegu area. One hundred and eleven healthy dogs which were 8 months old or more were examined by means of autopsy from September to November, 1993. All of the worms in small intestine were collected and preserved in 10% formalin solution. The worms were cleared in the lactophenol solution and examined by stereomicroscope and lightmicroscope. The result obtained were summarized as follows : Fifty three dogs(47.7%) out of the 111 experimental dogs were infected with small intestinal nematodes. Hookworm infection was recognized in 30(27.7%) dogs. All of the hookworms were identified morphologically as Ancylostoma caninum which had 3 pairs of ventral teeth, a pair of triangular dorsal teeth and a pair of centrolateral teeth. No Uncinaria stenocephala infection was detectable. Roundworm infection was recognized in 37(33.3%) dogs. Twenty one(18.9%) dogs were infected with Toxascaris leonina which had characteristic dorsal lips, paired spicules and a simple tail without process. The individual worm burden of the infected dogs ranged from one to 361 with mean of 25.5%. Among them 0~358(mean $17.8{\pm}30.66$) were Ancylostoma caninum, 0~18(mean $1.6{\pm}3.44$) were Toxocara canis and 0~60(mean $6.0{\pm}12.05$) were Toxascaris leonina. Single infection with Ancylostoma caninum, Toxocara canis and Toxascaris leonina were observed in 16(14.4%) dogs, in 9(8.1%) dogs, and in 11(9.9%) dogs respectively. Double infection with Ancylostoma caninum plus Toxascaris leonina and Toxocara canis plus Toxascaris leonina occurred in 5(0.5%) and in 3(2.7%) dogs respectively. Triple infection with Ancylostoma caninum plus Toxocara canis plus Toxascaris leonina occurred in 7(6.3%) dogs.

• 한국CDE학회논문집
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• 제18권4호
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• pp.275-282
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• 2013

This paper proposes a method for human identification using teeth contours extracted from dental images that are captured from the frontal views of subjects each of who opens his or her mouth slightly. Each dental image has a black-colored region containing the subject's teeth contours which are usually different from subject to subject. This means that this black-colored region has bio-mimetic information useful for human identification. The basic idea of the method is to extract the upper and lower teeth contours from the dental image of each subject and to encode their geometric patterns using a back-propagation neural network model. After acquiring 400 teeth images form 10 university students, we used 300 images for the training data of the neural network model and 100 images for its verification. Experimental results have shown that the proposed neural network-based method can be used as an alternative solution for identification among a small group of humans with a low cost and simple setup.

• 대한치과보철학회지
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• 제57권1호
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• pp.42-48
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• 2019

하악 편측에 소수의 잔존치만 있는 증례에서 통상적인 국소의치로 수복하는 경우에 충분한 유지 및 저항을 얻기 어렵고, 무치악 치조제의 흡수에 따른 의치의 안정성 저하로 인해 잔존지대치에 유해한 힘이 가해질 것으로 예상된다. 이러한 경우에 다수의 임플란트를 이용한 고정성 보철로 수복하게 되면 여러 가지 장점이 있을 수 있으나, 해부학적, 경제적 요인에 의해 치료 방법으로 선택이 제한될 수 있다. 이에, 적은 수의 임플란트를 전략적 위치에 식립하여 임플란트 보조 국소의치(Implant-assisted removable partial denture, IARPD)의 제작을 대안으로 고려해 볼 수 있다. 본 증례보고에서는 전통적인 가철성 국소의치만으로는 적절한 지지, 유지, 안정이 어려운 두 임상증례에서 소수의 임플란트를 전략적 위치에 식립하고 로케이터(Locator) 부착장치를 적용하여 3년간 경과관찰을 통해 좋은 임상 결과를 확인하였기에 이를 보고하고자 한다.

• 대한소아치과학회지
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• 제34권3호
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• pp.490-497
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• 2007

치아종은 느린 성장을 보이는 치성기원 양성종양으로 법랑질, 상아질, 백악질 그리고 치수조직으로 구성되어 있으며 치성종양의 22%를 차지한다. 치아종은 일반적으로 무증상이기 때문에 치과 치료를 위한 일상적인 방사선 검사에서 발견되는 경우가 많고, 영구치의 맹출지연이나 유치의 만기잔존 및 인접치아의 위치이상 등을 유발한다. 치아종의 방사선학적 소견은 일반적으로 방사선 투과성대로 둘러싸인 방사선 불투과성 물질이 관찰된다. 그러나 방사선 불투과상이 분명하지 않거나 매우 작은 치아종의 경우 치아의 상과 중첩될 수 있으며 발생 초기에 발육하는 힘에 의해 치아 맹출이 방해받을 수 있다. 따라서 맹출지연을 보이는 치아를 발견하면 즉시 치근단 방사선 사진을 촬영하여 방사선 불투과성 물질이 관찰되는지 확인한다. 또한 치아종은 치아의 발육과 같은 단계를 거친다. 따라서 맹출지연을 보이는 경우 첫 방사선 검사시에는 치아종이 관찰되지 않았으나 시간이 지나면서 관찰될 수 있으므로 치근단 방사선 사진에서 불투과성 물질이 확인되지 않더라도 지속적인 방사선 검사를 시행해야 한다. 본 증례는 영구치의 맹출지연 및 회전을 주소로 원광대학교 치과병원 소아치과에 내원한 환아에 있어서 치아의 정상적인 맹출 및 배열을 방해하는 작은 치아종을 발견하여 외과적으로 적출하였기에 이를 보고하는 바이다.

• 대한치과보철학회지
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• 제33권2호
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• pp.210-230
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• 1995

To compare the wear resistance of four kinds of commercial acrylic resin teeth [SR-Orthosit PosterioresR(Ivoclar Co., Liechtenstein), Endura PosteriorR(Shofu Inc. Japan), trubyte IPN teethR(Dentsply International Inc., York,), Trubyte BiotoneR(dentsply Inermational Inc. Brazil) by means of the toothbrush abrasion method, the artificial resin teeth were embedded in epoxy resin with the occlusal surfaces aligned in one plane for a total of 40 blocks. There after, each lock was mounted in the arm of the toothbrush abrasion machine(K 236, Japan). Wear measurements were made on the three preconditioned states. Those were as follows : no treatment specimens, thermocycled specimens, and thermocycled specimens which were immeresed applied load of 400g during the buring the brushing cycle. At the end of the 30,000-stroke cycle, each specimen was removed, and weighed. The microhardness of four kinds of commercial resin teeth were determined by means of microhardness tester. Microhardness tests were performed on te no treatment specimens, thermocycled specimens, and thermocycled specimens with immersion in the denture cleansing solution. Finally, the comparison of thermal properties were perfomed using differential scanning calorimeter(DSC-1500). The following results were obtained : 1. In the case of no treatment teeth, the wear amount of endura Posterior(EN) was the greatest among the others(p<0.01), and the wear amount of three kinds of artificial resin teeth was increased in the order of Trubyte IPN teeth(IN), Trubyte Biotone(BN), SR-Orthosit Posteriores(SN) but there was no statistic significance(p>0.01). 2. The wear amount of Trubyte IPN teeth(IT) and Trubyte Biotone(BT), was increased due to thermocycling effect, but that of Endura Posteriores(ET) was decreased conversely(p<0.01). 3. Except for the SR-Orthosit Posteriores(STC), the wear amount of three kinds of artificial resing teeth(that is, ETC, ITC, BTC) was increased due to denture cleansing solution$(Cledent^R)$, but there was no statistic significance(p>0.01). But the wear amount of the SR-Orthosit Posteriores(STC) was the greatest among the others(p<0.01). 4. The wear amount of toothbrush was the greatest in case of contact with occlusal surface of SROrthosit Posteriores resin teeth(p<0.01). 5. the microhardness values(KHN) of the SR-Orthosit Posteriores was the highest among the experimental artificial resin teeth(p<0.01). 6. There was no relationship between microhardness valuse(KHN) and wear amount of four kinds of experimental artificial resin teeth(p<0.01). 7. The differential canning calorimetric property of four kinds of artificial resin teeth did not show endothermal or exothermal peak in the range of $100^{\circ}C$

• Applied Microscopy
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• 제46권2호
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• pp.93-99
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• 2016

The thickness of specimen is an important factor in microscopic researches. Thicker specimen contains more information, but it is difficult to obtain well focused image with precise details due to optical limit of conventional microscope. Recently, a microscope unit that combines improved illumination system, which allows real time three-dimensional (3D) image and automatic z-stack merging software. In this research, we evaluated the usefulness of this unit in observing thick samples; Golgi stained nervous tissue and ground prepared bone, tooth, and non-transparent small sample; zebra fish teeth. Well focused image in thick samples was obtained by processing z-stack images with Panfocal software. A clear feature of neuronal dendrite branching pattern could be taken. 3D features were clearly observed by oblique illumination. Furthermore, 3D array and shape of zebra fish teeth was clearly distinguished. A novel combination of two channel oblique illumination and z-stack imaging process increased depth of field and optimized contrast, which has a potential to be further applied in the field of neuroscience, hard tissue biology, and analysis of small organic structures such as ear ossicles and zebra fish teeth.

• 대한치과보철학회지
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• 제57권1호
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• pp.81-87
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• 2019

다운증후군은 영구치의 법랑질과 상아질 층이 얇아서 치아가 작고, 부정교합의 발생가능성이 높다. 특히 치주염의 빈도가 높으며 작고 불규칙한 치아 및 좁은 구개로 인한 부정교합의 발생가능성이 높고, 발치 지연이나 유치잔존의 가능성이 있다. 이런 다운증후군 환자에게 있어서 상악 이중관을 이용한 의치는 한가지 좋은 치료선택이 될 수 있다. 상악 이중관을 이용하면 교차교합을 해결해 줄 수 있을 뿐만 아니라 의치의 지지 및 유지에도 도움이 된다. 또한 향후 지대치 발치시에 수리가 쉬운 장점이 있다. 본 증례에서 다운증후군과 치과공포증을 가진 26세 여자 환자에서 상악에 잔존유치가 존재하고, 치아 갯수가 작으며, 잔존치아 대부분의 경우 법랑질저형성증(Enamel hypoplasia)을 보여주고 있었다. 또한 좁은 구개로 인한 전후 및 측방으로 교차교합 상태였다. 상악에는 전후 및 좌우 반대교합(Cross bite)을 극복하기 위해서 이중관을 이용한 가철성의치로 수복을 하였고, 하악의 경우는 마취없이 가능한 법랑질에 변연을 형성하여 전치부는 라이네이트로 구치부는 지르코니아로 수복을 시행하였다. 그 결과 발치와 마취없이 반대교합을 해결하였으며, 심미성과 기능성의 개선 및 환자의 삶의 질을 개선할 수 있었다.

• 대한소아치과학회지
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• 제24권1호
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• pp.19-26
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• 1997

Odontodysplasia is a rare developmental anomaly of tooth formation in which hard tissue are affected. Odontodyplasia affects the primary and permanent dentitions in the maxilla, mandible, or both jaws. The maxilla is involved twice as often as the mandible. The condition is more common in female than in male patients and in the anterior than in the posterior regions. The clinical manifestation of odontodysplasia are hypoplasia and hypocalcification of the enamel and dentin of affected teeth. Teeth tend to be small and discolored, with short roots and widely open pulp canal. Delayed eruption of affected teeth with abscess formation is common. Radiographically teeth assume a faint radiolucent image ("ghost teeth"). enamel and dentin appear thin and are similar in radiodensity. The pulp chambers are often larger than normal, calcifications(pulp stone and denticle) are found within them. The etiology of regional odontodysplasia is unknown. However, several causes have been discussed, including somatic mutation, local circulatory disorders, local trauma, failure of migration and differentiation of neural crest cells, local infection.