• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.148-149
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• 2001

Protein inhibitors are proteins or peptides capable of inhibiting catalytic activities of proteolytic enzymes. They are grouped primarily as either serine, cysteine, aspartic or metallto-proteinase inhibitors. Pretense inhibitors have been hewn since the end of the last century in nematodes and human blood serum, and their ubiquitous distribution in microorganisms, animals and plants has been widely documented. (omitted)

• 정보보호학회논문지
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• 제8권4호
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• pp.33-42
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• 1998

본 논문은 그동안 알려지지 않았던 SKIPJSCK알고리즘의 암호화 방식을 소개하고 입출력 변화 공격 관점에서의 공격가능성을 검토한다. 기본적인 특징으로 키 설정시간이 거의 없고, 적은 양의 메모리를 이용하여 라운드 함수의 비선형 논리를 설계하였으며, 전체적으로 8비트 프로세서로 구현하는 것이 용이하다. 라운드 함수 G의 DC특성으로부터 16라운드 SKIPJSCK 2$^{24}$ 개의 선택 평문이 있으면 2$^{32}$의 계산량으로 키를 찾을 수 있다.

• 수산해양기술연구
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• 제51권1호
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• pp.79-85
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• 2015

Three specimens of larvae (5.2~7.8 mm in standard length (SL)), of the family Scombridae, were collected from the southeastern waters off Jeju Island in August, 2014. These specimens were identified to Katsuwonus pelamis have melanophores distributed on the 1st dorsal-fin spines and post ventral margin on caudal peduncle. An analysis of the cytochrome oxidase submit I (COI) sequences (652 base pairs) of mitochondrial DNA showed that our specimens must be K. pelamis, because their sequences were concordant with those of the adult K. pelamis (genetic distance= 0.000~0.002), followed by Auxis rochei (genetic distance= 0.061~0.063) and Euthynnus affinis (genetic distance= 0.077~0.079). During the larval stage, K. pelamis differed from Thunnus spp. species in melanophores distribution period of 1st dorsal-fin spines, lower jaws and ventral margin on caudal peduncle. In conclusion, occurrence of K. pelamis larvae means Korean waters are potential spawning ground of K. pelamis.

• 한국수산과학회지
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• 제53권2호
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• pp.165-173
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• 2020

Fish roe is among the most valuable food resources produced by fisheries. Raw fish roe requires processing for conversion into hygienic, marketable, and consumer-acceptable forms. In this study, to investigate the food compositional characteristics of various types of fish roe, we applied vacuum freeze-drying to prepare fish roe concentrates (FRCs) from roe of Alaska pollack Theragra chlcogramma, bastard halibut Paralichythys olivaceus, and skipjack tuna Katsuwonus pelamis. The FRC yield ranged from 22.7 to 26.7 g/100 g roe. The major constituents of FRCs were protein (65.4-79.6%), moisture (2.8-6.2%), lipids (8.5-18.3%), and ash (4.8-7.2%). Potassium, sulfur, sodium, and phosphorus were the major mineral elements of FRCs, and the major amino acids were aspartic acid (9.0-10.4 g/100 g protein), glutamic acid (13.2-14.5 g/100 g protein), lysine (8.4-8.6 g/100 g protein), and leucine (8.3-9.7 g/100 g protein). Vacuum freeze-dried FRCs differed among fish species in terms of amino acid composition and electrophoresis protein band distribution. Therefore, FRCs are an excellent source of protein nutrition and an appropriate protein fortification material in human foods or animal feed.

• 한국수산과학회지
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• 제21권2호
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• pp.85-96
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• 1988

Purification and some properties of alkaline proteinases in the pyloric caeca of skipjack, Katsuwonus vagans, were investigated. Four alkaline proteinases, temporarily designated proteinases I, II, III and IV, were identified from the tissue extract of the pyloric caeca by ammonium sulfate fractionation, DEAE-Sephadex A-50 chromatography, and Sephadex G-100 and G-200 gel filtration. Result of disc-polyacrylamide gel electrophoretic analysis showed that the purified proteinases II and III were homogenous with the yields of $1.5\%\;and\;1.2\%$, and those specific activities were increased to 33 to 37 fold over that of the crude enzyme solution, respectively. Molecular weight of the proteinases II and III determined by sephadex G-100 gel filtration were 28,500 and 24,200, respectively. The optimum conditions for the caseinolytic activity of the two enzymes were pH 9.6 and $48^{\circ}C$. The reaction rates of the two alkaline proteinases were constant to the reaction time to 80 min in the reaction mixture of $3.4{\mu}g/ml$ of enzyme concentration and $2\%$ casein solution. The Km values against casein substrate determined by the method of Lineweaver-Burk were $0.56\%$ for proteinase II and $0.30\%$ for proteinase II. The proteinases II and III were inactivated under the presence of $Ag^+,\;Hg^{2+},\;Ni{2+},\;Fe^{2+},\;and\;Cu^{2+}$, and but activated by $Mn^{2+}\;and\;Ca^{2+}$ and markedly inhibited by the soybean trypsin inhibitor and N-p-toluenesulfonyl-L-lysine chloromethyl ketone. Therefore, the proteinases II and III were found to be a group of serine proteases and assured to be trypsin-like proteinases.

• 수산해양교육연구
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• 제9권2호
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• pp.236-245
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• 1997

암화전(岩和田) 어업협동조합에 속하는 어선어업의 조업형태는 1인승과 2인승으로 구분 할 수 있으며, 2인승 어선에 의한 어획이 1인승어선의 어획보다 높다는 것을 알 수 있었다. 2인승어선이 어획한 7어종으로부터 어획금액이 높은 어종별로 어선을 분류하여, 다시 이것들을 판별 함수로서 분류한 결과, 4 그룹으로 분류되었다. 그 그룹은, 방어류 및 오징어류를 주로 어획하는 Gl그룹, 방어류 및 가다랭이 참치류를 주로 어획하는 G2그룹, 오징어 및 가다링이 참치류를 주로 어획하는 G3그룹, 그리고 기타어류 및 문어를 주로 어획하는 G4그룹이다. 또, 7어종의 어획금액에 대해서 주성분분석을 하여, 제1주성분에서 제3주성분까지의 주성분득점으로 부터 그룹을 고찰한 결과, G1, G2 및 G3그룹은 동일한 어업형태로 판단되었고, G4그룹만이 다른 어업형태인 것을 알 수 있었다.

• 수산해양기술연구
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• 제51권3호
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• pp.414-423
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• 2015

Fishing trend and characteristics of Korean tuna purse seine fishery in the Pacific Ocean were investigated using logbook data compiled from captain onboard and the statistical data from 1980 to 2013. The historical catch of this fishery had sharply increased since mid-1980s, and it has shown fluctuations with about 2-3 hundred thousands, whereas the catch per number of vessel has steadily increased with fluctuations since commencing this fishery. As for the proportion of catch by set type, unassociated type had increased from the mid-1980s to the end of 1990s, and then has decreased up to 2010s. Associated type had decreased continually to the end of 1990s, however, it started to increase since the beginning of 2000s. As for the catch proportion of set type by main species, those of skipjack tuna and bigeye tuna showed higher in the associated type, whereas that of yellowfin tuna has the highest proportion in the unassociated type. Fishing distribution of Korean tuna purse seine fishery was concentrated on the area of $5^{\circ}N{\sim}10^{\circ}S$ and $140^{\circ}E{\sim}180^{\circ}$ through the decades. The monthly catch distribution by longitudinal zone of Korean tuna purse seine fishery expanded the most further to the eastward in September to October.

• 수산해양기술연구
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• 제41권4호
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• pp.263-270
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• 2005

This paper summarizes characteristics of the Korean tuna purse seine fishery in the Western and Central Pacific Ocean (WCPO). Major fishing area for the Korean tuna purse seine fleet consisted of the waters of Micronesia, Papua New Guinea and Solomon Islands and adjacent high seas between $130^{\circ}E-180^{\circ}$ The center of the Korean tuna fishing ground was in the vicinity of $180^{\circ}$ during the El - $Ni{\tilde{n}}$o event but was formed in $150^{\circ}-160^{\circ}$E during the La - $Ni{\tilde{n}}o$ event. As compared with other major purse seine fleets in WCPO, the Korean fleet set more on unassociated schools of tuna, while Japan, USA and Chinese - Taipei fleets accounted for more than 50% of sets on log - associated schools. In general, CPUES for log - associated schools were higher than those for unassociated schools and catch composition showed that yellowfin ratio was higher in unassociated shools than log - associated schools. Length frequency represented higher percentage of mid - sized yellowfin than Japanese and USA fleets. Daily set numbers and CPUE were high before 7 am and remained almost constant from 7 am to 3 pm but decreased rapidly after 3 pm and 5 pm, respectively. Catch composition per set indicated that pure skipjack school was caught most frequently, followed by mixed school and pure yellowfin school as the least, but CPUE was highest for the mixed school.

• 한국식품조리과학회지
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• 제24권3호
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• pp.349-357
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• 2008

In an effort to augment extractability of carnosine and anserine at the levels of pro-oxidants such as iron and protein in Tuna boiled extracts(Skipjack, Yellowfin and Bigeye), we assessed the effects of heated and ion exchange chromatography(IEC) and ultrafiltration(UF) using a MW 500 cut-off(500 MWCO). We also evaluated the antioxidant activity of these extracts processed as free radical scavengers and reducing agents. Tuna boiled extracts of dark and ordinary muscle protein and total iron were reduced, whereas carnosine and anserine concentrations and antioxidant activity were increased. The carnosine and anserine concentrations of the ion exchange and permeate UF(IEC-UF) extracts were higher than those observed in the heated and permeate UF(heat-UF), whereas the protein and total iron contents were lower than that observed in the heat-UF. The quantity of carnosine and anserine in ordinary muscle was higher than that detected in dark muscle. HPLC analysis and SDS-PAGE were shown to removes the effect of UF on high molecular weight impurities in the tuna boiled extracts. The major free amino acids(FFAs) from Skipjack, Yellowfin and Bigeye tuna IEC-UF extracts were anserine, histidine and carnosine. These three peptides constituted more than 80～85%. of the detected amino acid. The IEC-UF treated ordinary muscle extracts evidenced the highest levels of DPPH radical scavenging activity and the highest levels of reducing power among the various extracts. The IEC-UF extracts evidenced a DPPH radical scavenging effect equal to that of 1mM ascorbic acid.

• 한국식품과학회지
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• 제9권1호
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• pp.47-60
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• 1977

85% 나트륨 tripolyphosphate와 15% 나트륨 hexametaphosphate로 조성된 $7{\sim}10%$ 인산수용액을 미리 조리하기 전에 어체의 무게가 약 $4{\sim}10%$ 증가될 때까지 다량어육질에 주입하였다. 이실험은 $45{\sim}68kg$과 $7.3{\sim}0.5kg$ 크기의 황다랭이(Thunnus albacares)와 $4.5{\sim}5.0kg$ 크기의 가다랭이(Euthynnus pelamis)를 이용하여 산업규모의 다랑어 가공공장에서 처리하였는데 이 실험(實驗)에서 나타나는 인산염처리(燐酸鹽處理) 결과는 다음과 같았다. 1. 큰 황다랭이는 수율이 약 $5{\sim}8%$ 증가하였고 다소 수분함량이 높았다. 2. 조그만 황다랭이는 수율이 약 $3{\sim}8%$ 증가하였다. 3. 가다랭이는 수율이 $1{\sim}4%$ 증가하였다. 4. 생산품의 색과 맛은 극히 조금 좋아졌다.