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Effects of Extraction Method on the Histidine Containing Low Molecular Weight Peptide and Pro-oxidants Contents of Tuna Boiled Extracts  

Kang, Ok-Ju (Department of Food and Nutritional Sciences Kyungnam University)
Publication Information
Korean journal of food and cookery science / v.24, no.3, 2008 , pp. 349-357 More about this Journal
Abstract
In an effort to augment extractability of carnosine and anserine at the levels of pro-oxidants such as iron and protein in Tuna boiled extracts(Skipjack, Yellowfin and Bigeye), we assessed the effects of heated and ion exchange chromatography(IEC) and ultrafiltration(UF) using a MW 500 cut-off(500 MWCO). We also evaluated the antioxidant activity of these extracts processed as free radical scavengers and reducing agents. Tuna boiled extracts of dark and ordinary muscle protein and total iron were reduced, whereas carnosine and anserine concentrations and antioxidant activity were increased. The carnosine and anserine concentrations of the ion exchange and permeate UF(IEC-UF) extracts were higher than those observed in the heated and permeate UF(heat-UF), whereas the protein and total iron contents were lower than that observed in the heat-UF. The quantity of carnosine and anserine in ordinary muscle was higher than that detected in dark muscle. HPLC analysis and SDS-PAGE were shown to removes the effect of UF on high molecular weight impurities in the tuna boiled extracts. The major free amino acids(FFAs) from Skipjack, Yellowfin and Bigeye tuna IEC-UF extracts were anserine, histidine and carnosine. These three peptides constituted more than 80～85%. of the detected amino acid. The IEC-UF treated ordinary muscle extracts evidenced the highest levels of DPPH radical scavenging activity and the highest levels of reducing power among the various extracts. The IEC-UF extracts evidenced a DPPH radical scavenging effect equal to that of 1mM ascorbic acid.
Keywords
anserine; carnosine; ion exchange chromatography; tuna boiled extracts; ultrafiltration;
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  • Reference
1 Oyaizu M. 1988. Antioxidative activities if browning products of glucosamine fractionated by organic solvent and thin-layer chromatography. Nippon Shokuhin Kogyo Gakkaishi 35(1):771-775   DOI
2 William HS, Stanford M. 1954. The free amino acid of human blood plasma. J Biological Chemistry 206(1):915-926
3 Yeargans GS, Seidler NW. 2003. Carnosine promotes the heat denaturation of glycated protein. Biochem. and Biophysic. Research Communica 300(1):75-80   DOI   ScienceOn
4 Decker EA, Crum AD. 1993. Antioxidant activity of carnosine in cooked ground pork. Meat Science 34(7):245-253   DOI   ScienceOn
5 Kanner J, Harel S, Jaffe R. 1991. Lipid peroxidation of muscle food as affected by NaCl. J Agric Food Chem 39(4):1017-1024   DOI
6 Maikhunthod B, Intarapichet KO. 2005. Heat and ultrafiltration extraction of broiler meat carnosine and its antioxidant activity. Meat Sci 71(2):364-374   DOI   ScienceOn
7 Decker EA, Faraji H. 1990. Inhibition of lipid oxidation by carnosine. J Am. Oil. Chem Soc 6(10):650-652
8 Shimada K, Fujikawa K, Yahara K, Nakamura T. 1992. Antioxidative properties of xanthan on the antioxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chemistry 40(1):945-948   DOI
9 Choi YJ, Kim IS, Lee KW, Kim GB, Lee NG, Cho YJ. 1996. Available components of cooking drips, dark muscle, head and raw vicera from skipjack. J Korean Fish. Soc. 29(5):701-708
10 Parker JR. 1966, Spectrophotometric Determination of Carnosine and Anserine in Muscle. Anal Chem 38(10):1359-1362   DOI   ScienceOn
11 McManus IR. 1957. Some metabolic precursors of the N-1-methyl group of anserine in the rat. J Biological Chemistry 225(1):325-334
12 Oh KS, Lee EH, Kim MC, Lee KH. 1987. Antioxidative activities of skipjack meat extract. Bull Korean Fish Soc 20(5):441-446
13 Stookey LL. 1970. Ferrozine: a new spectrophotometric reagent for iron. Anal Chem 42(7):779-781   DOI
14 Spackman DH, Stein WH, Moore S. 1958, Automatic recoding apparatus for use in the chromatography of amino acids. Anal Chem 30(1):1190-1206   DOI
15 Chan KM, Decker EA, Means WJ. 1993. Extraction and activity of carnosine, a naturally occuring antioxidant in beef muscle. J Food Sci 58(1) : 1-7   DOI   ScienceOn
16 Hipkiss AR, Brownson C, Carrier MJ. 2001. Carnosine, the antiageing, anti-oxidant dipeptide, may react with protein carbonyl groups. Mech Age Develop 122(1):1431-1445   DOI   ScienceOn
17 Intarapichet OK, Maikhunthod B. 2005. Genotype and gender difference in carnosine extracts and antioxidant activities of chicken breast and thigh meats. Meat Science 71(4):634-642   DOI   ScienceOn
18 Lowry OH, Rosebrough NJ, Farr AL. Randall RJ. 1951. Protein measurement with folin phenol reagent. J Biological Chemistry 193(2):265-275
19 Laemmli UK. 1970. Cleavage of structure proteins during the assembly of the head of bacteriophage T4. Nature 227 (5259):680-686   DOI   ScienceOn
20 Wu HC, Shiau CY, Chen HM, Chiou TK. 2002. Antioxidant activity of carnosine anserine some free amino acids and their combination. J Food Drug analysis 11(2):148-153
21 Hector G, Nelson B, Gladys PR. 1995. Improved rapid method for the isolation, purification and identification of collagen glycosides. J chromatography 732(2):385-389   DOI
22 Kim WS. 2003. Antioxidant activity and safety evalution of carnosine, anserine and homocarnosine isolated from spent hen. Doctorate thesis. The Duksung women's university of Korea. pp 16-22
23 Gopalakrishnan J, Decker EA, Means WJ. 1999. Antioxidant activity of mechanically separated pork extracts. Meat Science 52(1):101-110   DOI   ScienceOn