• Korean Journal of Acupuncture
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• v.38 no.2
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• pp.62-74
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• 2021

Objectives : The aim of this study is to analyze the effect of acupuncture and the details of acupuncture treatment methods on skin wrinkles. Methods : Search was conducted in Pubmed, KISS, and NDSL databases for acupuncture studies on skin wrinkles. The detailed therapeutic techniques of acupuncture used for skin wrinkles and the effect of acupuncture on skin wrinkle improvement were analyzed. Then, the study results using the same indicator were compared through meta-analysis in order to compare the effects of acupuncture with the control group. The quality of randomized controlled trials (RCTs) was assessed using the risk of bias (ROB) assessment tool (Ver. 1.0, Cochrane Collaboration). Results : A total of 10 RCTs and 19 case series were included in this study. The most frequently used therapeutic technique for skin wrinkle improvement was Microneedle therapy system (MTS, n=19), and they were mostly used with aesthetic solutions (13 out of 19 studies). Skin wrinkle related indexes were most commonly used to evaluate skin wrinkle improvement (n=14). Panax Ginseng pharmacopuncture showed the most remarkable effect in improving the range of skin wrinkles and the depth of skin wrinkles. MTS+epidermal growth factor improved the levels of skin hydration, skin elasticity, and variation of skin pigment the most. MTS+Endothelial precursor cell improved the number of skin melanin the most. Three RCTs included in the meta-analysis showed moderate ROB on average. Conclusions : Acupuncture treatment was effective in improving skin wrinkles. However, a precise study design using unified indicators and appropriate controls needs to be performed in further studies in order to establish more convincing evidence of the effectiveness of acupuncture for improving skin wrinkles.

• Toxicological Research
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• v.35 no.4
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• pp.353-359
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• 2019

The purpose of this study was to investigate the effect of $7-MEGA^{TM}$ 500 on the improvement of skin aging in an UVB-induced photo-aging model of hairless mice. The dorsal skin of hairless mice was exposed to UVB three times a week for 12 weeks to induce skin wrinkle. After inducing the wrinkle, $7-MEGA^{TM}$ 500 was orally administered once a day for 4 weeks. Skin thickness, skin barrier function, and wrinkle indicators were improved by treatment with $7-MEGA^{TM}$ 500. Both gene and protein expression levels of MMP-3 and c-Jun in skin were significantly decreased by $7-MEGA^{TM}$ 500. Therefore, the intake of $7-MEGA^{TM}$ 500 is thought to have a positive effect on the improvement of skin aging, although further studies are needed.

• Journal of Physiology & Pathology in Korean Medicine
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• v.32 no.5
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• pp.321-327
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• 2018

The purpose of this study was to investigate the effects of Cuscuta chinensis Lamark ethanol extract (CL) on wrinkle improvement. Cuscuta chinensis Lamark is known to contain dried saccharide, alkaloids, flavonoids, lignans and rein glycoside as major components of dried mature seeds of Cuscuta japonica Choisy. In this study, we evaluated the anti-wrinkle effects of CL and investigated bio-markers (e.g ; MMP-1, MMP-3, MMP-9, TIMP-1, type I procollagen) associated with skin wrinkle improvement. We tested the anti-wrinkle effect of CL using human fibroblast called CCD-986Sk cell. We observed an increase in MMPs, TIMP-1, and type 1 pro-collagen CL in CCD-986Sk cells irradiated with UVB at an intensity of $2mJ/cm^2$ for 60 seconds. As a result, CL decreased UVB-induced MMPs levels and mRNA expressions in CCD-986Sk cell. The levels and mRNA expressions of type I procollagen and TIMP-1 were increased by CL. These results suggest that CL has activities on improvement of skin wrinkle, which is induced by UVB radiation. Taken together, this study proposed the possibility of developing herbal medicine and functional herbal cosmetic materials with wrinkle-improving effects of Cuscuta chinensis Lamark.

• KSBB Journal
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• v.26 no.3
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• pp.255-259
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• 2011

This study was to suggest the method for wrinkle improvement using Mugil cephalus extract. In order to evaluate the cosmetic product made of M. cephalus extract, the experiments were conducted with subjects and skin states for 4 weeks. Effect of cosmetics containing M. cephalus extract on the water holding capacity and evaporation of the women skin were investigated. The water holding capacity was increased after 1 h of application. But after 2 h, it was decreased. In the case of the water evaporation, it was increased with the increase of time. On the clinical trial, it was also found that water, oil, and rough level on the women skin were increased and wrinkles were improved. Side effects were also not detected during the application and treatment of cosmetics. Based on this result, the application of M. cephalus extract had positive effects on the improvement of wrinkles and skin in women. Therefore, it will be used to develop the wrinkle improvement therapy for women.

• BMB Reports
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• v.46 no.9
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• pp.465-470
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• 2013

Bone morphogenetic proteins (BMPs) have diverse and important roles in the proliferation and differentiation of adult stem cells in our tissues. Especially, BMPs are well known to be the main inducers of bone formation, by facilitating both proliferation and differentiation of bone stem cells. Interestingly, in skin stem cells, BMPs repress their proliferation but are indispensable for the proper differentiation into several lineages of skin cells. Here, we tested whether BMP antagonists have an effect on the prevention of wrinkle formation. For this study we used an in vivo wrinkle-induced mouse model. As a positive control, retinoic acid, one of the top anti-wrinkle effectors, showed a 44% improvement compared to the non-treated control. Surprisingly, bone morphogenetic protein receptor 1a extracellular domain (BMPR1a-ECD) exhibited an anti-wrinkle effect which was 6-fold greater than that of retinoic acid. Our results indicate that BMP antagonists will be good targets for skin or hair diseases.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.12 no.4
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• pp.336-342
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• 2019

With the entry of an aging society, the average life span of accreditation has been extended. Therefore, interest in the appearance of men and women in modern society has increased. It is the wrinkles of the face that can judge the most outwardly. People tend to have various kinds of treatments to have a clean, wrinkle-free and resilient healthy skin regardless of sex. There is a lot of practice of lifting procedures in one of the procedures. A suture using a melting thread is a method of lifting by squeezing it into the skin as a non-incision type centering on a region where the thread can be fixed in the skin by injecting it into the subcutaneous fat layer. To evaluate the lifting efficacy of Cog - type suture for the improvement of skin wrinkles, preclinical experiments were conducted. We developed a wrinkle evaluation program using Labview. Data from preclinical experiments were used at 8 weeks after suturing. The average wrinkle depth was 415.6 mm in the control group. At 8 weeks, the depth of wrinkles was deepened to 888.3mm due to the aging process of the control group. On the other hand, the depth of the wrinkles before surgery was 640.3 mm in the suture group. It was confirmed that the depth of wrinkles decreased to 566.5mm at 8th week after the suture operation.

• Journal of Acupuncture Research
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• v.27 no.1
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• pp.101-107
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• 2010

Objectives : The purpose of this study was to estimate the wrinkle treatment effect of Miso Facial Acupuncture with the facial skin photographing system. Methods : Six middle-aged women with no diseases were recruited. Miso Facial Acupuncture, a total ten times was performed three times a week. The facial skin photograph of each participant was checked two times-pretreatment, posttreatment-using the facial skin photographing system. Both squares and counts of wrinkles in the facial 3 parts were measured for each one. Results : Both squares and counts of wrinkles were decreased on average. The improvement of lower eyelid were the best of all. And the wrinkles of outside of lip were reduced a little. Conclusions : in this study, we could evaluate the wrinkle treatment effects of Miso Facial Acupuncture by an objective and scientific method. Miso Facial Acupuncture has notable effect in wrinkle treatment. As though we had not wide experiences in this treatment, more researches are needed.

• Proceedings of the SCSK Conference
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• 2003.09b
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• pp.253-267
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• 2003

Inositol and phytic acic extracted from rice bran were investigated for applying cosmetics. Skin lotions containing 0∼3.0wt% inositol and 0∼1.5wt% phytic acid were applied respectively, to the arm skins of 45 Asian women 20'-40's for 7 weeks. Improvement on moisture was evaluated. In addition, improvements on sebum, elasticity, and wrinkle were examined after applying placebo, Inositol and phytic acid-containing skin lotions for face, respectively. For 1.0 wt% inositol resulted in 19% increase of moisture. The wrinkle reduction and elasticity improved 12.4% and 17.0% on average, respectively. Applying 0.5wt% phytic acid resulted in 71.6% increase the moisture. Improvements on wrinkle and elasticity were 15.9% and 21.9% respectively. Applying inositol or phytic acid regardless of dry or oily, resulted in sebum value recovery to that of the normal skin after 2- 4 weeks. Inositol is inferior to phytic acid in improvenients of the skin, and phytic acid is not suitable to sensitive skin. So 0-0.5wt% of phytic acid were added to 1.0wt% inositol and similar experiments were carried out. In case of added 0.1wt% phytic acid, moisture increased 63.8% approximately. Improvements on elasticity and reduction on wrinkle were 17.2% and 17.4%, respectively. Both skin types were turned to normal skin type after 2 weeks. It could improve the skin condition when used inositol added phytic acid. The optimized concentration of phytic acid was 0.10wt% with 1.0wt% of inositol for synergic effect.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.448-456
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• 2003

The prime objective of many facial wrinkle-reduction treatments is to achieve visible improvement. Thus the visibility of before/after treatment differences is often part of an efficacy assessment. This paper investigates whether the background knowledge of the people acting as observers in such assessments is of impact on the results: e.g. the subjects themselves are familiar with their faces, skin professionals have much experience in judging skin quality, and thus both might be more sensitive to small changes. In a clinical study 44 Female subjects were regularly treated during a period of 12 weeks with one of three wrinkle-reduction methods: K, Land M (placebo). Photographs were taken before treatment and at 6 and 12 weeks. The photographs were judged by 3 types of observers:ㆍ24 Lay observers were given the 0＆6-week and the 0＆12-week photo pairs of all subjects to indicate the one with the least wrinkles in a two-alternative forced-choice (TAFC) procedure.ㆍThe subjects themselves were given the 0＆6-weel and the 0＆12-week pair of their own photos (8 replications) to indicate the photo with the least wrinkles (TAFC).ㆍA trained panel of skin professionals (N=3) each gave 9-point Fitzpatrick wrinkle-severity scores for all individual 0-week and 12-week photos. We found that the lay observers perceived the same differences as the subjects themselves: significant improvements after 12 weeks for treatment K (p<0.0005 and p=0.005, respectively), no visible effects for treatments Land M, and, most importantly, a significant difference between treatments K and M/placebo (p=0.02 and p=0.04, respectively). Also the trained panel found this difference between K and M (p=0.013), but here it was due to a significant deterioration over time of the 'placebo-treated' wrinkles (M, p=0.03). Thus in conclusion we have found no indications that extra knowledge - in the form of familiarity with the own face or in the form of professional training - results in the identification of more treatments that show significantly visible wrinkle-reduction.

• Journal of the Korean Applied Science and Technology
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• v.36 no.3
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• pp.853-865
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• 2019

In this study, the skin permeability was measured by adding skin penetrating peptides, arginine oligomers R4(tetra-D-arginine), R6(hexa-D-arginine) to little skin-permeable wrinkle improvement peptides GHK, GHK-Cu, and Pal-GHK liposomes, and the results were analyzed by the following six cases. (1) In cases where only wrinkle improvement peptides GHK, GHK-Cu, and Pal-GHK were contained liposomes; the final cumulative permeations in 24 hours were 6.05%, 7.4%, and 8.83% respectively. (2) In cases where arginine oligomers R4, R6 were added to GHK liposomes; the final cumulative permeations in 24 hours were 13.63% and 7.68%. (3) In cases where R4, R6 were added to GHK-Cu liposomes; the final cumulative permeations in 24 hours were 15.46% and 8.64%. (4) In cases where R4, R6 were added to Pal-GHK liposomes; the final cumulative permeations in 24 hours were 16.9% and 10.67%. (5) In cases where R4 were added to GHK, GHK-Cu, and Pal-GHK liposomes; the final cumulative permeations in 24 hours were 13.63%, 15.46%, and 16.9% respectively. (6) In cases where R6 were added to GHK, GHK-Cu, and Pal-GHK liposomes; the final cumulative permeations in 24 hours were 7.68%, 8.64%, and 10.67% respectively. This experiment showed that skin absorption of GHK was increased by copper ion (Cu2+) and palmitic acid and skin absorption of wrinkle improvement peptides was enhanced by cell penetrating peptides, and R4 showed higher effect than R6 in GHK, GHK-Cu and Pal-GHK. Through this process, we propose broad use and application in wrinkle improvement functional cosmetics by presenting the optimal conditions for increasing skin absorption of GHK, GHK-Cu, thus maximizing its efficacy.