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http://dx.doi.org/10.12925/jkocs.2019.36.3.853

Enhancement of Skin Permeation of Wrinkle Improvement Peptides GHKs Using Liposomes Containing Skin Penetrating Peptides  

Park, Su In (Department of Senior Healthcare, majored in Cosmetic Pharmacology, Eulji University)
An, Gyu Min (Department of Senior Healthcare, majored in Cosmetic Pharmacology, Eulji University)
Kim, Min Gi (Department of Senior Healthcare, majored in Cosmetic Pharmacology, Eulji University)
Heo, Soo Hyeon (Department of Senior Healthcare, majored in Cosmetic Pharmacology, Eulji University)
Shin, Moon Sam (Department of Senior Healthcare, majored in Cosmetic Pharmacology, Eulji University)
Publication Information
Journal of the Korean Applied Science and Technology / v.36, no.3, 2019 , pp. 853-865 More about this Journal
Abstract
In this study, the skin permeability was measured by adding skin penetrating peptides, arginine oligomers R4(tetra-D-arginine), R6(hexa-D-arginine) to little skin-permeable wrinkle improvement peptides GHK, GHK-Cu, and Pal-GHK liposomes, and the results were analyzed by the following six cases. (1) In cases where only wrinkle improvement peptides GHK, GHK-Cu, and Pal-GHK were contained liposomes; the final cumulative permeations in 24 hours were 6.05%, 7.4%, and 8.83% respectively. (2) In cases where arginine oligomers R4, R6 were added to GHK liposomes; the final cumulative permeations in 24 hours were 13.63% and 7.68%. (3) In cases where R4, R6 were added to GHK-Cu liposomes; the final cumulative permeations in 24 hours were 15.46% and 8.64%. (4) In cases where R4, R6 were added to Pal-GHK liposomes; the final cumulative permeations in 24 hours were 16.9% and 10.67%. (5) In cases where R4 were added to GHK, GHK-Cu, and Pal-GHK liposomes; the final cumulative permeations in 24 hours were 13.63%, 15.46%, and 16.9% respectively. (6) In cases where R6 were added to GHK, GHK-Cu, and Pal-GHK liposomes; the final cumulative permeations in 24 hours were 7.68%, 8.64%, and 10.67% respectively. This experiment showed that skin absorption of GHK was increased by copper ion (Cu2+) and palmitic acid and skin absorption of wrinkle improvement peptides was enhanced by cell penetrating peptides, and R4 showed higher effect than R6 in GHK, GHK-Cu and Pal-GHK. Through this process, we propose broad use and application in wrinkle improvement functional cosmetics by presenting the optimal conditions for increasing skin absorption of GHK, GHK-Cu, thus maximizing its efficacy.
Keywords
Wrinkle improvement peptide; Skin penetrating peptide; Liposome; Enhancement of skin absorption; GHKs;
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Times Cited By KSCI : 3  (Citation Analysis)
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