• 대한화장품학회지
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• 제44권2호
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• pp.111-116
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• 2018

최근 화장품 시장에서 안티폴루션 효능이 있는 화장품은 새로운 피부 건강을 위한 해결책으로 나타나고 있다. 외부(대기) 오염물질에 의해 피부의 산화 기전을 매개하는 주요 원인 인자들로 오존, UV, 미세먼지 및 담배연기 등을 꼽을 수 있다. 담배 연기의 노출은 직 간접적으로 피부 표피 내 지질의 산화를 야기한다. 피부의 지질 산화에 의해 squalene과 squalene monohydroperoxide의 비율 변화가 발생하고, malondialdehyde (MDA)가 지질산화 산물로 생성된다. 따라서, 본 연구에서는 담배연기 노출 시 발생하는 피부 산화에 대하여 레드비트를 함유하는 화장품이 방지할 수 있는 효능이 있는지에 대하여 MDA의 생성량으로 관찰하였다. 시험대상자 전박부를 세 영역(음성대조, 양성대조, 시험제품)으로 나누어 각 지름 3.3 cm의 원으로 구획하고, 15분간 담배연기에 노출 후 피부 표면으로부터 지질막을 걷어낸 후 TBARS assay를 통하여 MDA를 정량 하였다. 음성대조(무도포, 미노출)에 비해 양성대조(무도포, 담배연기 노출)의 MDA 생성량이 3.7배 증가된 결과로, 오염물질인 담배연기에 의한 피부의 산화적 손상을 확인하였다. 반면에 레드비트를 함유하는 화장품을 미리 도포한 영역은 양성대조에 비해 MDA 생성량이 25% 감소하는 결과를 나타내었다. 결론적으로, 담배연기 노출은 피부표피 내 지질 산화를 야기하며, 레드비트를 함유하는 화장품이 이러한 대기환경오염으로부터 방어적 효과(안티폴루션 효과)를 보이는 것을 확인하였다.

• 한방안이비인후피부과학회지
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• 제25권1호
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• pp.1-11
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• 2012

Objective : Paeoniae radix alba(PRA) can enrich the blood and regulate menstruation, astringe yin and arrest sweating, calm the liver and arrest pain. This study was designed to investigate effects of PRA on skin whitening and elasticity using melanoma cells. Methods : In this experiment, effect of PRA on cell viability, inhibition of melanin synthesis and inhibitory effect on tyrosinase and elastase. Results : 1. More than $1,000\;{\mu}g/ml$ of PRA treated group showed lowered proliferation rates significantly compared to non-treated control group. 2. All of treated groups were lower levels of melanin synthesis respectively. 3. PRA did not show inhibitory effect on tyrosinase activities in vitro. But, PRA suppressed tyrosinase activities in B16F10 cells significantly. 4. PRA suppressed elastse type 1 activities in dose-dependent manner in vitro. But, PRA slightly suppressed elastase type 4 activities in vitro, and PRA also slightly suppressed elastase activities in vivo. Conclusion : These results suggest that PRA can inhibit melanin synthesis through ihhibitory action on tyrosinase activity and inhibt elastase activity, and also suggest that these results can be used for the study on maintaining skin whitening or elasticity.

• Journal of Pharmaceutical Investigation
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• 제25권1호
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• pp.47-54
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• 1995

Transdermal patch formulations of estradiol to treat post-menopausal symptoms and prevent osteoporosis in women were developed and evaluated for the permeation characteristics through the excised hairless mouse abdorminal skin and the uterotropic effect on the ovariectomized rabbits. The design of patch formulations was optimized by varying several formulation parameters, such as type of enhancers, amount of enhancers, amount of drug loading and coating thickness. Compared to a commercially available transdermal product, several patch formulations showed the similar skin permeation profiles (following zero-order kinetics), but their skin permeation rates were lasted for the longer period (a week). In one-week uterotropic efficacy test in the ovariectimized rabbits, the selected patch formulations showed the positive effect in atrophy of the urogenital epithelium. The mean values of uterus weight in rabbits after application of patches containing estradiol were much higher than those in control group (containing no drug).

• 한국식품영양과학회지
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• 제32권3호
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• pp.444-449
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• 2003

To investigate the long term effect of buchu (Chinese chives) diet on ROS formation in the liver and stin tissue of ICR mice, one of control, 2% or 5% buchu-added diet was fed to ICR mice for 12 months. Superoxide anion (O2ㆍ), hydrogen peroxide($H_2O$$_2$) and hydroxyl radical (ㆍOH) contents were measured in cytosol, microsome, mitochondria of liver and skin of mice, respectively. Behu diet showed a significant decrease of superoxide anion, hydrogen peroxide and hydroxyl radical contents in liver and skin tissues compared to control diet, and this effect is especially higer at 5% than at 2% buchu diet level. ICR mice showed an age-dependent increase in ROS contents, while the group fed buchu diet decreased its ROS contents significantly and ROS contents of liver appeared to be 2 fold higher than skin. The results of the present study suggest that antioxidative components and sulfur-compounds in buchu diet appear to be responsible for the inhibition of ROS formation in ICR mice.

• 한국축산식품학회지
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• 제32권3호
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• pp.308-315
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• 2012

This study was conducted to examine the effects of egg shell membrane hydrolysates (ESMH) on skin whitening, wound healing, and UV-protection. ESMH was divided into three groups by molecular weight (Fraction I: above 10 kDa of ESMH, Fraction II: 3 kDa-10 kDa of ESMH, Fraction III: below 3 kDa of ESMH). As a result, all of ESMHs showed over 90% of protein contents. The wound healing experiment using HaCaT cells showed that the fraction I was slightly superior to other fractions depending on the concentration though it was not significantly different. In the experiments of inhibition of tyrosinase and L-3,4-dihydroxyphenylalanine (L-DOPA) oxidation to verify the L-DOPA whitening effect, the whole ESMH (before fractioning) showed a similar amount of inhibition effect with arbutin (control). In the inhibition of melanin formation in B16-F1 melanoma cells, the fraction I showed a high inhibitory effect. In the experiment for protecting the skin from ultraviolet rays using HaCaT cells, all the fractions showed a higher rate of cell viability than the control. In conclusion, this study confirmed that the cosmetic effects of ESMHs such as skin whitening, wound healing, and UV-protection, which were divided depending on the molecule weight. We could confirm that the possibility of ESMHs as a material for functional cosmetics.

• 대한본초학회지
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• 제37권6호
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• pp.53-59
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• 2022

Objectives : Cleanser is the most basic tools used in daily skin care. I made a cold process soap using Castanea crenata inner shell (CCIS). I'd like to study the effect of face wash using CCIS cold process (CP) soap on the skin. Methods : The same water and towel were used at the same place in order to examine the skin change state immediately before and after washing face. The skin test was performed before and after 10 minutes washing the face in order to remove these external environmental factors, such as lifestyle, alcohol, tobacco, and stress etc. As a control group, the cases of washing with water and foam cleanser (F/C), which is a popular commercial product, were set. Three cases were compared and analyzed. Results : In the case of water washing, there was a significant decrease in oil (U-zone) but that was not good effect. In the case of F/C and CCIS washing, there were significant decrease in oil (T-zone), pores and pigmentation, but the significance was bigger in CCIS washing. In the case of pores, there was a significant decrease in all three cases. Conclusion : In the case of F/C and CCIS washing, it showed the effect of reducing T-zone oil, pores and pigmentation. But the significance was greater in CCIS case than in F/C case. CCIS cp soap is considered as a form of face wash that is suitable for skin care.

• 대한본초학회지
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• 제38권2호
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• pp.9-16
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• 2023

Objectives : Many people use natural soap instead of commercially available bar type general soap for skin care. They tend to prefer cp (cold process) type soaps that go through a sufficient maturation process in formulation. The purpose of this study was to investigate the efficacy of PCPS (placenta cp soap). Methods : The researcher selected 36 subjects, 28 as the experimental group and 8 as the control group. I asked participants to wash their face twice a day in the morning and evening, and the period was set for 6 weeks. The researcher had the people who were selected as the control group use a commercially available foam cleanser, meanwhile the experimental group use PCPS. They performed a skin test before the start of the test, and the change status was continuously investigated after 2 weeks, 4 weeks, and 6 weeks after using the soap. Results : In the case of U zone oil and pores, there was a significant decrease and in the case of skin tone, there was a significant increase in the group of using PCPS. In T zone oil change, there was no significant effect in both the experimental group and the control group. Conclusion : In the case of PCPS, it is recommended to use it in the effect of reducing the size of pores and increasing skin tone, but it can reduce u-zone oil content.

• 대한한의학방제학회지
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• 제31권3호
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• pp.183-191
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• 2023

Objective : UVB damages skin health by causing skin redness and intense inflammation, sunburn, and skin cancer. Paeoniae Radix Alba has been used to relieve gynecological symptoms, muscle spasms, and skin ailments. This study was conducted to confirm whether it has a protective effect against UVB photodamage. Methods : Ethanol extract of Paeoniae Radix Alba (PRA) was prepared by extracting 100 g Paeoniae Radix Alba in 1 L of ethanol for 48 h. Apoptosis was monitored by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and expression levels of apoptosis indicator proteins, and tyrosinase activity was measured with a colorimetric commercial kit. Results : In human keratinocyte HaCaT cells, PRA reduced UVB-induced cell death through apoptosis by inhibiting PARP cleavage and caspase-3 and -9. UVB-induced increase in cellular reactive oxygen species (ROS) was suppressed by PRA pretreatment. PRA also showed dose-dependent ABTS and DPPH radical scavenging activities. Furthermore, the inhibitory effect of tyrosinase activity by PRA was confirmed. Conclusion : These results demonstrated the protective role of PRA in UVB photodamage of human keratinocytes, mainly due to its antioxidant and antiapoptotic properties. We also suggest that PRA can be considered as an effective natural agent to prevent skin photodamage.

• 대한화장품학회지
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• 제42권4호
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• pp.413-420
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• 2016

하이드록시 산(hydroxy acid, HA)은 피부 각질층에 대한 박리효과로 노화방지와 피부 보습을 높이는데 효과를 보여, 피부 외용제와 화장품으로서 많은 활용을 하고 있다. 그 중에서 가장 효과적으로 빠른 시간 내에 나타나는 각질 박리는 화장품 제형 pH에 의해 효과를 보이는 것으로 많은 보고가 있다. 그러나 pH에 의한 자극, 부작용에 의한 염려로 인해 사용에 많은 어려움이 있다. 본 연구의 목적은 피부 각질층에 (1) 하이드록시 산의 농도와 (2) 종류, (3) pH를 변화시킨 화장품을 인체 피부에 도포하여 각질 박리 효과에 대한 영향을 측정하는 데에 있다. 건강한 성인 22명을 대상으로 하박 내측에 DHA (dihydroxyacetone), DC (dansyl chloride)로 피부 표면 각질을 염색하여, 시험제품을 도포한 뒤 각질박리 효과를 측정하였다. (1) GA (glycolic acid)의 농도에 따라 각질 박리 효과는 농도 의존적으로 증가하는 것으로 나타났다. (2 )하이드록시 산의 종류에 따라 pH를 산성과 중성으로 제조한 제품을 대상으로 각질 박리 효과를 측정한 결과, 중성 pH의 GA는 각질 박리 효과가 나타나지 않았다. 이에 반해 SA (salicylic acid)는 산성 pH와 중성 pH에서 모두 통계적으로 유의한 각질 박리 효과가 나타났다. (3) 중성 pH의 SA는 DHA와 DC로 염색한 피부 표면에서 모두 우수한 각질 박리 효과를 나타내었다. 이러한 결과는 pH에 민감한 사람들에게 각질 박리 효과를 기대하는 화장품을 사용할 수 있는 기회를 제공하며, 피부 장벽의 손상 없이 안전한 화장품을 제조할 수 있을 것이라 사료된다.

• 대한한의정보학회지
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• 제15권1호
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• pp.85-91
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• 2009

Objectives: We investigated the influence of the neck acupressure massage and the neck cream including korean herbal extract on the neck wrinkles and blood circulation. Methods: Korean female($37.47{\pm}3.66$ years, n=18) aged 30-39 years were participated in this study. We measured the neck wrinkles with PRIMOS(GFMesstechnik GmbH, Germany) and blood circulation of neck and facial skin with Laser Doppler Imaging-Periscan PIM II(Perimed AB, Sweden). Results: The neck wrinkles were significantly decreased after applying neck cream. But when with the neck acupressure massage, the neck wrinkles were decreased 2-fold than applying only the neck cream. The skin blood circulation was significantly increased on the neck and facial skin immediately after the neck acupressure massage. But this effect was not significantly different after 8 weeks Conclusions: This result suggests that the neck acupressure massage can improve the anti-wrinkles effect of the neck cream including Korean herbal extract.