• Toxicological Research
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• v.21 no.2
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• pp.99-105
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• 2005

Photo-mutagenic compounds have been known to alter skin cancer rates by acting as initiators or by affecting subsequent steps in carcinogenesis. The objectives of this study are to investigate the utility of photo-chromosomal aberration (photo-CA) assay for detecting photo-clastogens, and to evaluate its ability to predict rodent photocarcinogenicity. Photo-CA assay was performed with five test substances that demonstrated positive results in photo-carcinogenicity tests: 8-Methoxypsoralen (photoactive substance that forms DNA adducts in the presence of ultraviolet A irradiation), chlorpromazine (an aliphatic phenothiazine an alpha-adrenergic blocking agent), lomefloxacin (an antibiotic in a class of drugs called fluoroquinolones), anthracene (a tricyclic aromatic hydrocarbon a basic substance for production of anthraquinone, dyes, pigments, insecticides, wood preservatives and coating materials) and Retinoic acid (a retinoid compound closely related to vitamin A). For the best discrimination between the test substance-mediated genotoxicity and the undesirable genotoxicity caused by direct DNA absorption, a UV dose-response of the cells in the absence of the test substances was firstly analyzed. All 5 test substances showed a positive outcome in photo-CA assay, indicating that the photo-CA test is very sensitive to the photo-genotoxic effect of UV irradiation. With this limited data-set, an investigation into the predictive value of this photo-CA test for determining the photo-carcinogenicity showed that photo-CA assay has the high ability of a test to predict carcinogenicity. Therefore, the photo-CA test using mammalian cells seems to be a sensitive method to evaluate the photo-carcinogenic potential of new compounds.

• Journal of the Korean Society of Radiology
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• v.11 no.5
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• pp.353-359
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• 2017

The sonophoresis, a representative low-intensity ultrasonic therapy, is a technique for delivering the drugs into the epidermis, dermis and skin appendages by using physical vibration and heat effects of the ultrasonic waves. Sonophoresis could increases the delivering and absorption efficiency of the drugs usually consisting of hydrophilic molecules and macromolecules. In addition, it has the advantage of being effective in delivering drugs with relatively large molecular sizes such as insulin or lipid. In this study, we proposed a multi-structure ultrasonic transducer assembly with a large-size single piezoelectric element and a drug delivery function at the treatment site for efficient sonophoresis treatment. Futhermore, a transducer assembly structure capable of raising and maintaining the temperature of the treatment site was proposed and evaluated for effectiveness. The transducer assembly proposed in this study is expected to improve the efficiency of sonophoresis by providing a constant amount of drug, and assisting drug delivery through heating the treatment site.

• Journal of Microbiology and Biotechnology
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• v.28 no.1
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• pp.109-114
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• 2018

Coxsackievirus Type B3 (CVB3) is an enterovirus that belongs to the Picornaviridae and causes various diseases such as myocarditis and hand-foot-mouth disease. However, an effective antiviral drug is still not developed. In this study, we looked for potential inhibitors of CVB3 replication by examining the survival of CVB3-infected HeLa cells. We detected an antiviral effect by cholic acid and identified it as a candidate inhibitor of CVB3 replication. Cholic acid circulates in the liver and intestines, and it helps the digestion and absorption of lipids in the small intestine. HeLa cells were cultured in 12-well plates and treated with cholic acid (1 and $10{\mu}g/ml$) and $10^6PFU/ml$ of CVB3. After 16 h post-infection, the cells were lysed and subjected to western blot analysis and RT-PCR. The production of the viral capsid protein VP1 was dramatically decreased, and translation initiation factor eIF4G1 cleavage was significantly inhibited by treatment with $10{\mu}g/ml$ cholic acid. Moreover, cholic acid inhibited ERK signaling in CVB3-infected HeLa cells. RT-PCR showed that the amounts of the CVB3 RNA genome and mRNA for the ER stress-related transcription factor ATF4 were significantly reduced. These results showed that cholic acid strongly reduced ER stress and CVB3 proliferation. This compound can be developed as a safe natural therapeutic agent for enterovirus infections.

• Archives of Plastic Surgery
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• v.41 no.6
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• pp.740-747
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• 2014

Background Autologous fat graft has become a useful technique for correction of acquired contour deformity in reconstructed breasts. However, there remains controversial regarding the efficacy and safety of the practice for reconstructive breast surgery. Methods A retrospective review was performed on 102 patients who had secondary fat grafting after breast reconstruction. Fat harvest, refinement and injection were done by Coleman's technique. All patients were followed up postoperatively within 1 month and after 6 months including physical examination and ultrasonography. In 38 patients, the reabsorption rate was calculated by serial changes of thickness between skin and pectoral fascia in the ultrasonic finding. Locoregional recurrence rate was compared with control group of 449 patients who had breast reconstruction without fat graft in the same time period. Results Average 49.3 mL fat was injected into each breast. The most common location of fat graft was upper pole, followed by axilla, lower and medial breasts. During 28.7 months of average follow-up period, 2.9% of total patients had symptoms of palpable mass on fat graft side and ultrasonography identified fat necrosis and cyst formation in 17.6% of the patients. Calculated fat reabsorption rate was 32.9%. Locoregional recurrence was occurred in 1 patient (0.9%) and the rate was not different significantly with control group (2%). Conclusions Although further studies are required to provide surgeons with definitive guidelines for the implementation of fat grafting, we propose autologous fat graft is an efficient and safe technique for secondary breast reconstruction.

• Atmosphere
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• v.27 no.3
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• pp.317-329
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• 2017

Ocean mixed layer (OML) depth affects diurnal cycle of sea surface temperature (SST) induced by change of solar radiation absorption and heat budget in ocean. The diurnal SST variation can lead to convection over the ocean, which can impact on localized precipitation both over coastal and inland. In this study, we investigate the OML characteristics affecting the diurnal cycle of SST for the Korean Peninsula and surrounding areas. To analyze OML characteristics, HYCOM oceanic mixed layer depth (MLD) and wind field at 10 m from ERA-interim during 2008~2016 are used. In the winter, MLD is deeply formed when the strong wind field is located on perpendicular to continental slope over deep seafloor areas. Besides, cooling SST-induced vertical mixing in OML is reinforced by dry cold air originated from Siberia. The OML in summer is shallowly distributed about 20 m. In order to estimate the impact of OML model in high resolution NWP model, four experimental simulations are performed. At this time, the prognostic scheme of skin SST is applied in NWP to simulate diurnal SST. The simulation results show that CNTL (off-OML) overestimates diurnal cycle of SST, while EXPs (on-OML) indicate similar results to observations. The prediction performance for precipitation of EXPs shows improvement compared with CNTL over coastal as well as inland. This results suggest that the application of the OML model in summer season can contribute to improving the prediction for performance of SST and precipitation over coastal area and inland.

• Archives of Plastic Surgery
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• v.45 no.6
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• pp.564-571
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• 2018

Background Chronic wounds occur due to failure of the normal healing process, associated with a lack of deposition of cellular components and a suitable microenvironment such as the extracellular matrix (ECM). Acellular dermal matrix (ADM) is viewed as an ECM substitute, and a paste-type ADM has recently been introduced. We hypothesized that CGPaste, an injectable paste-type ADM, could serve as a scaffold and promote wound healing. Methods We retrospectively studied seven patients in whom CGPaste was applied between 2017 and 2018, who had pressure ulcers, necrotizing fasciitis, diabetic foot ulcers, traumatic defects, and osteomyelitis. The goal of applying CGPaste was to achieve complete wound healing with re-epithelialization or growth of granulation tissue, depending upon the wound bed status. CGPaste was injected based on the wound size along with the application of a dressing. Results Four of the seven patients showed granulation tissue on their wound bed, while the other three patients had a bony wound bed. The mean wound area was $453.57mm^2$ and the depth was 10.71 mm. Wound healing occurred in five of the seven patients (71.43%). The mean duration of complete healing was 2.4 weeks. Two patients showed failure due to paste absorption (29.57%); these patients had wound beds comprising bone with relatively large and deep wounds ($40{\times}30$ and $30{\times}20mm^2$ in area and 15 and 10 mm in depth). Conclusions CGPaste is an effective option for coverage of small and deep chronic wounds for which a flap operation or skin grafting is unfeasible.

• The Journal of Korean Physical Therapy
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• v.15 no.3
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• pp.153-165
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• 2003

This study was carried out to evaluate the body fat breakdown during topical seaweed extract treatment and in combination with the iontophoresis method to enhance skin absorption of lipolytic compounds. Of 14 female obesity subjects, 7 treated a gel types of seaweed extract only(control group) and 7 treated in combination with the iontophoresis(experimental group). Topical treatment of each sample was designed for 2 weeks, treated with 2 times a day about $10m{\ell}$ on the abdomen, triceps and thigh, respectively. Experimental group treat with the iontophoresis during 5 minutes immediately after topical treatment. After one and two weeks of topical treatment, measured body fat, skinfolds thickness, body circumference respectively. Then venous blood samples were taken and analyzed serum lipids. The results were as follows; 1) There were decrement of body weight, $\%$ of body fat, abdomen, triceps and thigh circumference and abdomen, triceps and thigh thickness between pre and post treatment in both group. These decrement were predominant in the iontophoresis treated group. Especially, there were $16\%$ of body fat decrement in the experimental group after two weeks iontophoresis treatment(control group VS experimental group = $33.54{\pm}2.70$ VS $28.37{\pm}3.38$, p<0.01). 2) There were decrement in total CHOL, LDL, TRIG and increment in HDL in both group. These phenomena were predominant in the iontophoresis treated group. CHOL decrement in the experimental group were significant after two weeks iontophoresis treatment(control group VS experimental group = $190.24{\pm}34.36$ VS $157.76{\pm}24.45$, p<0.001). These results suggest that the topical treatment of seaweed extract effective for fat breakdown and maximized in the combination with the topical seaweed extracts treatment and the iontophoresis.

• Journal of radiological science and technology
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• v.15 no.2
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• pp.25-30
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• 1992

With transmitted dose through chest which has the problem of wide variations in absorption, simple film/screen combination method makes it diffucult to image lung field, mediastinum and retrocardiac areas. In order to overcome this, it is very common to use the high kilovoltage technique in diminishing the differences between high and low contrast. We have been adopting this method at department of diagnostic radiology, Seoul National University Hospital. To compare the image of it with that of low kilovoltage technique, we did radiographic tests using beans on the skin. We marked off into three anatomical categories such as lungs, mediastinum and near diaphragm, then attached a bean on a marked area at random. In order to compare with high and low, we took a radiography of high($120{\sim}140\;kVp$) and low($70{\sim}90\;kVp$) kilovoltage tehchniques, respectively at the same time. We have done experiments 320 cases. We evaluated the results of test in response to sensitivity(true positive) and specificity(true negative). In evaluating, we gave them points from 1 to 5 according to true or false. With given points by a radiologist having much experiences, we could acquire the percentage of sensitivity and specificity. The percentage made us to get the schematic table of ROC curve of those two methods. Consequently, high kilovoltage technique appeared 18% better than low kilovoltage technique for detecting beans with our apparatus.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.41 no.7
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• pp.463-468
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• 2017

For transdermal drug delivery, needless injection system is composed of laser and microjet injector. Main mechanism of microjet injector is the laser-induced bubble. Nd:YAG and Er:YAG laser are used as a power source. Laser parameters such as pulse duration and wavelength are considered, which are core parameters to control the bubble motion. The Nd:YAG laser, pulse duration is short than bubble life time making cavitation like bubble while in Er:YAG laser, long pulse duration and high absorption in water drive bubble as a boiling bubble. Detailed motion of bubble and microjet is captured by the high speed camera. So it is observed that microjet characteristics are determined by the bubble behavior. The performance of drug delivery system is evaluated by fluorescent staining of guinea pig skin.

• Smart Structures and Systems
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• v.17 no.5
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• pp.819-835
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• 2016

In this paper, the activities carried out within the EU funded Clean Sky Joint Technology Initiative (JTI GRA) Project and aimed at developing a morphing flap, are illustrated. The reference device is a regional aircraft single slotted flap, enhanced with deforming capabilities to obtain improved hyper-lift performance. The design started with the identification of the internal architecture, intended to allow camber variations. A concentrated-hinge architecture was selected, for its ability to fit different curvatures and for the possibility of easily realizing an "armadillo-like" configuration, then avoiding the use of a complicate deformable skin. The flap layout is made of segmented ribs, elastically hinged each other and span-wise connected by conventional spars. Relative rotations of the rib elements are forced by SMA structural actuators, i.e., cooperating in the external loads absorption. Super-elastic SMA are used to make up recovery elastic elements, necessary to regain the original shape after activation. These further elements in turn contribute to the overall flap rigidity. After assessing the hinge number and the size of the SMA active and passive elements, the advanced design phase was dealt with. It was aimed at solving manufacturing issues and producing the executive drawings. The realized demonstrator was finally tested in lab conditions to prove its functionality in terms of whether target shape actuation or attained shape preservation under loads. On the basis of the numerical results and the experimental outcomes, precious hints were obtained for further developments of the concept.