• BMB Reports
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• v.33 no.1
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• pp.69-74
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• 2000

The Arabidopsis thaliana S-Adenosylmethionine decarboxylase (AdoMetDC) cDNA ($GenBank^{TM}$ U63633) was cloned, then the AdoMetDC protein was expressed and purified. The purified AdoMetDC was inactivated by salicylaldehyde in a pseudo first- order kinetics. The secondorder rate constant for inactivation was 126 $M^{-1}min^{-1}$ with the slope of n=0.73, suggesting that inactivation is the result of the reaction of one lysine residue in the active site of AdoMetDC. Site-specific mutagenesis was performed on the AdoMetDC to introduce mutations in conserved $lysine^{81}$ residues. These were chosen by examination of the conserved sequence and proved to be involved in enzymatic activity by chemical modification. Changing $Lys^{81}$ to alanine showed an altered optimal pH. The substrate also provided protection against inactivation by salicylaldehyde. Considering these results, we suggest that the $lysine^{81}$ residue may be involved in catalytic activity.

• YAKHAK HOEJI
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• v.28 no.3
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• pp.129-138
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• 1984

Specific [$^{3}H$] ouabain binding and $Ca^{2+}$ -uptake were measured to elucidate the role of high affinity [$^{3}H$] ouabain binding site in rat cardiac myocytes which contain 65% of rod cells. High affinity [$^{3}$H] ouabain binding site, which is about 3% of total pump sites, with apparent dissociation constant ($K_{D}$) of $1.1{\times}10^{-7}M$ and maximum binding site concentration (Bmax) of 1.2 pmol/mg protein ($1.754{\times}10^{5}cells$) were identified. At the concentration of $10^{-7}M$ to $10^{-4}M$, ouabain produced concentration dependent increase in $Ca^{2+}$-uptake of myocytes. The effect of ouabain on $Ca^{2+}$-uptake was not effected by membrane depolarization (elevated K+ in incubation medium) or verapamil. These results suggest that in rat ventricular myocytes the ouabain receptor complex to high affinity site may increase Na+ - $Ca^{2+}$ exchange across the sarcolemmal membrane by inhibition of Na+, K+ - ATPase.

• Earthquakes and Structures
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• v.13 no.1
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• pp.17-27
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• 2017

In this study, the characteristics of site amplification at seismic observation stations in Japan were estimated using the attenuation relationship of each station's response spectrum. Ground motion records observed after 32 earthquakes were employed to construct the attenuation relationship. The station correction factor at each KiK-net station was compared to the transfer functions between the base rock and the surface. For each station, the plot of the station correction factor versus the period was similar in shape to the graphs of the transfer function (amplitude ratio versus period). Therefore, the station correction factors are effective for evaluating site amplifications considering the period of ground shaking. In addition, the station correction factors were evaluated with respect to the average shear wave velocities using a geographic information system (GIS) dataset. Lastly, the site amplifications for specific periods were estimated throughout Japan.

• Journal of the Earthquake Engineering Society of Korea
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• v.9 no.4 s.44
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• pp.29-42
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• 2005

Total 350 borehole profiles were selected from the database of borehole logs in Seoul, for the site-specific seismic evaluation at two 4km${\times}$4km plain areas. Equivalent-linear site response analyses for the selected 350 sites were conducted based on shear wave velocity (Vs) Profiles, which were determined from the N-Vs correlation established using borehole seismic testing results in the inland areas of Korea. Most sites were categorized as site classes C and D based on the mean Vs to 30 m in depth (Vs30) ranging from 250 to 550 m/s. The she periods of the plains in Seoul ranging between 0.1 and 0.4 sec were significantly lower than those of the western US, from which the site coefficients in Korea were derived. For plains in Seoul, the site coefficients, Fa's and Fv's specified in the Korean seismic design guide, underestimate the ground motion in short-period (0.1-0.5 sec) band and overestimate the ground motion in mid-period (0.4-2.0 sec) band, respectively, because ol the differences in the geotechnical conditions between Seoul and the western US, although the Fa's in several sites overestimate the motion due to the base Isolation effect resulted from the soft layer in soil deposit.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.8 no.4
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• pp.25-35
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• 2013

The main objective of this study is to investigate the structural relation between antecedents and consequences of web sites' trust of venture company. It examines consumer perceptions trust in a web site and address following research questions; what factors influence consumer trust in a web site and what specific web site cues are associated with trust, satisfaction, and intention? We test our hypothesis in a empirical data from 123 consumers across 3 web sites of venture companies. By factor analysis, the results show that web site characteristics are such as design, business function, interface, technology, community, and contents of each web site. We also find that web site characteristics such as design, interface, technology, and community can influence significantly satisfaction of web site, and design and contents of web site can influence consumer's trust. And the results show that trust influence behavioral intention through the satisfaction. The results offer important implication for web site strategies of venture company that include the manipulation of factors influencing web site trust. And the future directions of the present research are discussed.

• Journal of the Korean Society of Radiology
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• v.9 no.5
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• pp.269-277
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• 2015

In recent years, concern about the decommissioning of nuclear power plants has been growing according to the nuclear power plants aging. Although site reuse is one of the most important issues in doing decommissioning of nuclear power plants in view of the internal affairs, there is currently no specific standard of the site release criteria for site reuse in the Republic of Korea. In this study, we analyzed the international safety standards for the site release(IAEA's Safety Guide No. WS-G-5.1) and the present domestic condition. Also, we studied the site release criteria and real life examples about advanced countries such as United states and Europe, which already have experience of decommissioning or site release. As a results of the study, we suggested proper standards and future consideration to establish site release criteria. This will be used for preliminary data for establishing the domestic site release criteria after the decommissioning of nuclear power plants.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.22 no.3
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• pp.31-54
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• 2019

The purpose of this study is to present the basic data for establishing the management plan of forest ecosystem in Honam-Jeongmeak and Geumnamhonam-Jeongmaek by surveying and analysing the vascular plants distributed in Jangansan(A-site), Palgongsan(B-site), Mandeoksan(C-site), Chuwolsan (D-site), Cheonunsan(E-site) and Jeamsan(F-site). The numbers of flora were 813 taxa including 115 families, 406 genera, 700 species, 4 subspecies, 94 varieties and 15 forms. There were 500 taxa in A-site, 427 taxa in B-site, 402 taxa in C-site, 449 taxa in D-site, 345 taxa in E-site and 524 taxa in F-site. The rare plants were 12 taxa including Paeonia japonica, Iris minutoaurea, Chloranthus fortunei and so forth. In IUCN Red List categories, there were 1 taxon of EN, 2 taxa of VU, 8 taxa of LC and 1 taxon of DD. The Korean endemic plants were 20 taxa including Salix koriyanagi, Thalictrum actaefolium var. brevistylum, Saussurea pseudogracilis and so forth. The specific plants by floristic region were 108 taxa including 58 taxa of grade I, 30 taxa of grade II, 16 taxa of grade III, 2 taxa of grade IV and grade V, respectively. The plants adaptable to climate change were 18 taxa including 11 taxa of southern plant and 7 taxa of northern plants. The naturalized plants were 49 taxa including Rumex nipponicus, Brassica juncea, Trifolium repens and so forth. The invasive alien plants were 4 taxa including Rumex acetocella, Solanum carolinense, Ambrosia artemisiifolia and Aster pilosus.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2001.09a
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• pp.28-35
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• 2001

Liquefaction potential on the specific site of nuclear power plant is analyzed and reviewed. The layered site fur this study consists of silt and sand. Based on the limited available soil data, maximum shear strength at critical locations using Seed & Idriss method and computer program SHAKE is calculated, and liquefaction potential is reviewed. Seismic input motion used fur the assessment of liquefaction is the artificial time history compatible with the US NRC regulatory Guider .60. Assessment results of the liquefaction are validated by analyzing to the other typical soil fecundations which can show the effects of foundation depth and soil data.

• Proceedings of the Korean Institute of Interior Design Conference
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• 1999.04a
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• pp.145-148
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• 1999

The contemporary society needs complex space for multiple function. Interior space plan should suggest the solution of the narrow site and process for multiple function through showing the examples of other functional spaces. The purpose of this study is choosing an express station which has wide site and cultural exchange function, while it is not used for specific function and then the waiting room of station adapted for cultural and multiple functional space.

• Journal of Microbiology
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• v.41 no.2
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• pp.169-173
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• 2003

A treatability investigation was conducted to determine if landfarming would be effective for the remediation of unsanitary landfill waste soils. Calculations based on biodegradable organic carbon contents and initial CO$_2$ evolution rates revealed that landfarming has a high potential for landfill site remediation and that the optimum strategy for bioremediation is site-specific.