• Journal of Korea Water Resources Association
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• v.45 no.8
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• pp.783-794
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• 2012

The analysis for unsteady flow is necessary to design the hydraulic structures affected by water level and discharge changes through time. The numerical model has been generally used for unsteady flow analysis, however it is difficult to acquire field data to calibrate and validate the numerical model. Even though it is possible to collect field data for some case, high cost and labor are required and sometimes it is considered that the confidence of measured data is very low. In this case, the experimental data for unsteady flow can be used to calibrate and validate the numerical model as an alternative. Therefore, the discharge-supply system which could generate various type of unsteady flow hydrograph was developed in this study. Also, the accuracy of the unsteady flow hydrograph generated by developed dischargesupply system in the experiment was evaluated by comparing with target hydrograph. Accuracy errors and Root Mean Square Error (RMSE) were analyzed for the rectangular-type hydrograph with sudden changes of flow, triangular-type hydrograph with short peak time, and bell-type flood hydrograph. As a result, the generating error of the discharge-supply system for the rectangular-type hydrograph was about 59% which was maximum error among various types. Also, it was represented that RMSE for the triangular-type hydrographs with single and double peaks were approximately corresponding to 10%. However, RMSE for the bell-type flood hydrograph was lower than 2%.

• The Korean Journal of Physiology and Pharmacology
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• v.14 no.1
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• pp.45-49
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• 2010

R-type $Ca_v2.3$ high voltage-activated $Ca^{2+}$ channels in peripheral sensory neurons contribute to pain transmission. Recently we have demonstrated that, among the six $Ca_v2.3$ isoforms ($Ca_v2.3a{\sim}Ca_v2.3e$), the $Ca_v2.3e$ isoform is primarily expressed in trigeminal ganglion (TG) nociceptive neurons. In the present study, we further investigated expression patterns of $Ca_v2.3$ isoforms in the dorsal root ganglion (DRG) neurons. As in TG neurons, whole tissue RT-PCR analyses revealed the presence of two isoforms, $Ca_v2.3a$ and $Ca_v2.3e$, in DRG neurons. Single-cell RT-PCR detected the expression of $Ca_v2.3e$ mRNA in 20% (n=14/70) of DRG neurons, relative to $Ca_v2.3a$ expression in 2.8% (n=2/70) of DRG neurons. $Ca_v2.3e$ mRNA was mainly detected in small-sized neurons (n=12/14), but in only a few medium-sized neurons (n=2/14) and not in large-sized neurons, indicating the prominence of $Ca_v2.3e$ in nociceptive DRG neurons. Moreover, $Ca_v2.3e$ was preferentially expressed in tyrosine-kinase A (trkA)-positive, isolectin B4 (IB4)-negative and transient receptor potential vanilloid 1 (TRPV1)-positive neurons. These results suggest that $Ca_v2.3e$ may be the main R-type $Ca^{2+}$ channel isoform in nociceptive DRG neurons and thereby a potential target for pain treatment, not only in the trigeminal system but also in the spinal system.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.30 no.4
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• pp.657-667
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• 2020

The Android framework allows apps to take full advantage of personal information through granting single permission, and does not determine whether the data being leaked is actual personal information. To solve these problems, we propose a tool with static/dynamic analysis. The tool analyzes the Source and Sink used by the target app, to provide users with information on what personal information it used. To achieve this, we extracted the Source and Sink through Control Flow Graph and make sure that it leaks the user's privacy when there is a Source-to-Sink flow. We also used the sensitive permission information provided by Google to obtain information from the sensitive API corresponding to Source and Sink. Finally, our dynamic analysis tool runs the app and hooks information from each sensitive API. In the hooked data, we got information about whether user's personal information is leaked through this app, and delivered to user. In this process, an automated Source/Sink classification model was applied to collect latest Source/Sink information, and the we categorized latest release version of Android(9.0) with 88.5% accuracy. We evaluated our tool on 2,802 APKs, and found 850 APKs that leak personal information.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2014.06a
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• pp.107-108
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• 2014

As the demand for precise navigation has increased, more focus is put on the precise positioning, RTK(Real Time Kinematics) which has been used in the surveying field. The Position of Single Reference Station RTK or two-way network RTK such as VRS (Virtual Reference Station) is accurate enough to be used as a main technology in land surveying, however its service area and number of users is limited and the users are assumed static. This characteristic is not suitable to the navigation, whose service target is infinite number of users moving over a wide area. One-way network RTK has recently been suggested as a solution for the precise navigation technique for the mobile user. This paper shows the performance prediction of the one-way network RTK such as MAC(Master-Auxiliary Concept), or FKP (Flachenkorrekturparameter). To show the performance variation by the rover position, we constructed a simulation data of users on the grid with 0.1 degree spacing between 36.5 and 37 degree latitude and between 127 and 127.5 degree longitude.

• Korean Journal of Pharmacognosy
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• v.48 no.3
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• pp.179-186
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• 2017

Pyrrosia lingua which belongs to Polypodiaceae has been used as a traditional medicine for the treatment of urinary system inflammation, urination disorder, and bronchitis. However, there are not enough phytochemical and pharmacological studies of P. lingua up to now. Here in this study, the protective effect of MeOH extract of whole plant of Pyrrosia lingua (MPL) against 2% glucose-induced toxicity was investigated using Caenorhabditis elegans (C. elegans) model system. We found that MPL significantly extended the lifespan of wild-type nematode under normal culture condition. MPL also effectively recovered the decreased lifespan caused by 2% glucose-toxicity. In addition, MPL efficiently attenuated the increased glucose concentration inside of nematode. Further studies evaluating diabetes-related factors revealed that MPL reduced both intracellular ROS and lipid accumulation which were up-regulated under 2% glucose supplement condition. Our data also showed that MPL improved the 2% glucose-induced shortened body movement of nematode. Lastly, we carried out genetic studies using several single gene knockout mutants to establish the possible target of MPL. Our results demonstrated that genes such as daf-2 and daf-16 were responsible for the protective activity of MPL against 2% glucose-induced toxicity. These results indicate that MPL exerts protective action against 2% glucose via regulation of insulin/IGF-1 sinaling pathway and FOXO activation.

• Journal of KIISE:Computing Practices and Letters
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• v.14 no.2
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• pp.246-250
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• 2008

This paper presents the design of network vulnerability analysis system which can integrate various vulnerability assessment tools to improve the preciseness of the vulnerability scan result. Manual checking method performed by a security expert is the most precise and safe way. But this is not appropriate for the large-scale network which has a lot of systems and network devices. Therefore automatic scanning tool is recommended for fast and convenient use. The scanning targets may be different according to the kind of vulnerability scanners, or otherwise even for the same scanning target, the scanning items and the scanning results may be different by each vulnerability scanner, Accordingly, there are the cases in which various scanners, instead of a single scanner, are simultaneously utilized with the purpose of complementing each other. However, in the case of simultaneously utilizing various scanners on the large-scale network, the integrative analysis and relevance analysis on vulnerability information by a security manager becomes time-consumable or impossible. The network vulnerability analysis system suggested in this paper provides interface which allows various vulnerability assessment tools to easily be integrated, common policy which can be applied for various tools at the same time, and automated integrative process.

• Herbal Formula Science
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• v.25 no.2
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• pp.135-143
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• 2017

Objectives : This trial aimed to determine if Wasong (Orostachys japonicus) extract can enhance immune system and is safe enough to be approved as a health functional food. Methods : Total 62 people, aged 45 and older, will be recruited to participate in a randomized, double-blind, placebo-controlled clinical trial. This study will compare Wasong extract and placebo. Wasong group will take 1g of Wasong extract, once a day, for 8 weeks. Placebo group will take 1g of crystalline cellulose as placebo, once a day, for 8 weeks. Outcomes will be measured at the baseline, the end of 4th week, and 8th week. Primary outcomes are the ratio of NK cells/total lymphocytes and the ratio of T-helper cells/T-suppressor cells. Secondary outcomes are total white blood cell count, the ratio of neutrophils, lymphocytes, and monocytes in total leukocytes, the ratio of total T cells, T-helper cells, T-suppressor cells, and B cells to lymphocytes, the amount of blood IgM, IgG, IgA, and cytomegalovirus (CMV) IgG, and blood metabolite target &global analysis. Results : This trial was approved by institutional review board of Pusan National University Korean Medicine Hospital (registry number: 2016006), and registered in Clinical Research information Service, one of WHO International Clinical Trials Registry Platform (registry number: PRE20161006-002). Recruitment opened in February 2017 and is supposed to be completed by August 2017. The result is expected to be published by June 2018. Conclusion : This trial will provide clinical information to determine the efficacy and safety of Wasong in enhancing immune system of middle-aged and older people.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1670-1674
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• 2007

Duffy binding protein (DBP) plays a critical role in Plasmodium vivax invasion of human red blood cells. We previously reported a single-chain antibody fragment (scFv) that was specific to P. vivax DBP (PvDBP). However, the stabilization and the half-life of scFvs have not been studied. Here, we investigated the effect of PEGylated scFvs on their biological activity and stability in vitro. SDS-PAGE analysis showed that three clones (SFDBII-12, -58, and -92) were formed as monomers (about 70 kDa) with PEGylation. Clone SFDBII-58 gave the highest yield of PEGylated scFv. Binding analysis using BIAcore between DBP and scFv showed that both SFDBII-12 and -58 were decreased approximately by two folds at the level of binding affinity to DBP after PEGylation. However, the SFDBII-92 clone still showed a relatively high level of binding affinity ($K_D=1.02{\times}10^{-7}\;M$). Binding inhibition assay showed that PEGylated scFv was still able to competitively bind the PvDBP and playa critical role in inhibiting the interactions between PvDBP protein expressed on the surface of Cos-7 cells and Duffy receptor on the surface of erythrocytes. When both scFvs and their PEGylated counterparts were exposed to trypsin, scFv was completely degraded only after 24 h, whereas 35% of PEGylated scFvs remained intact, maintaining their stability against the proteolytic attack of trypsin until 72 h. Taken together, these results suggest that the PEGylated scFvs retain their stability against proteolytic enzymes in vivo, with no significant loss in their binding affinity to target antigen, DBP.

• Journal of Microbiology and Biotechnology
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• v.19 no.3
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• pp.323-330
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• 2009

Nontuberculous mycobacteria (NTM) are a major cause of opportunistic infections in immunocompromised patients, making the reliable and rapid identification of NTM to the species level very important for the treatment of such patients. Therefore, this study evaluated the usefulness of the novel target genes tuf and tmRNA for the identification of NTM to the species level, using a PCRrestriction fragment length polymorphism analysis (PRA). A total of 44 reference strains and 17 clinical isolates of the genus Mycobacterium were used. The 741 bp or 744 bp tuf genes were amplified, restricted with two restriction enzymes (HaeIII/MboI), and sequenced. The tuf gene-PRA patterns were compared with those for the tmRNA (AvaII), hsp65 (HaeIII/HphI), rpoB (MspI/HaeIII), and 16S rRNA (HaeIII) genes. For the reference strains, the tuf gene-PRA yielded 43 HaeIII patterns, of which 35 (81.4%) showed unique patterns on the species level, whereas the tmRNA, hsp65, rpoB, and 16S rRNA-PRAs only showed 10 (23.3%), 32 (74.4%), 19 (44.2%), and 3 (7%) unique patterns after single digestion, respectively. The tuf gene-PRA produced a clear distinction between closely related NTM species, such as M. abscessus (557-84-58) and M. chelonae (477-84-80-58), and M. kansasii (141-136-80-63-58-54-51) and M. gastri (141-136-117-80-58-51). No difference was observed between the tuf-PRA patterns for the reference strains and clinical isolates. Thus, a diagnostic algorithm using a tuf gene-targeting PRA is a promising tool with more advantages than the previously used hsp65, rpoB, and 16S rRNA genes for the identification of NTM to the species level.

• Environmental and Resource Economics Review
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• v.20 no.3
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• pp.595-628
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• 2011

This study investigates the issues and suggests reform measures in applying CV methods to the Korea Development Institute's (KDI's) Preliminary Feasibility Test (PFT) of public projects. Most public projects on culture, science and environment evaluated under the PFT system belong to the category of "nonstandard" projects whose outputs are non-marketed, and CV is currently the main tool used for their benefit estimation. A careful discussion and investigation is recommended for the selection of target population, payment vehicle, and number of payment times. Operating expert reviews, focus group interviews, and pre-tests is highly recommended to reduce the potential bias involved in the CV studies. A single or double bounded dichotomous choice format is the most popular design of questionnaire, but we identify several undissolved issues in designing and implementing the format. Some other forms of inducing WTPs may still deserve our consideration. Various specifications of the WTP function need to be tried and tested based on their stability, in particular. Employing a nonparametric approach is also recommended. Treatments of 0 or negative WTPs and protest bids are shown to be the most serious issues that affect the estimation results significantly. We review diverse measures of handling those issues and summarize their advantages and shortcomings.